|
Grind365
AlwaysGrinding

Registered: 08/07/22
Posts: 448
Last seen: 1 year, 6 days
|
Re: Cultivation General Discussion [Re: cooleko]
#27953883 - 09/17/22 01:02 AM (1 year, 4 months ago) |
|
|
Quote:
SirPsycho said:
Quote:
Auron said:
Quote:
Smellyhobbit said:
Quote:
Auron said: I had some condensation on my plates so I placed a warm pot in them per the tek.
Now my transfers are sitting in moisture π‘ Did it fall down to the agar?
Sure did. Best thing to do is handle the condensation before you use them.
Will that ruin my growth?
No, the water in the plates is sterile.
The water might be but the contams it can move around aren't.Quote:
Nichrome said: The trick is making it look easy while you're doing it. Pretend important people are watching and they think you are a pro and paid you accordingly. Fake it till you make it.
Fake it till you make it is. Idk if this is a great tip but my mentor told me the same thing and I took it to heart.Quote:
cooleko said: Is this really a debate? You add water and powder into a media bottle, pressure cook it, and pour it into clean plates when it hits your favorite temperature. Making them is easy!
Then you open the plate in a clean environment and drop things into it to watch them grow. Using them is easy!
I have used cardboard boxes, insulated grocery bags, and cling wrap to make SABs and never had contamination in my agar unless I cloned mushrooms using their external bits, which was a conscious choice. It really is that easy!
Even buying them online I have had good success with with low (<5%) rates of contamination, so that is a gamble (but still easy)!
By your favorite temperature do you meant 120f any hotter and it's condensation heaven any cooler and I get chunks for days.
|
fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
|
Re: Cultivation General Discussion [Re: Wildcat1883] 1
#27953887 - 09/17/22 01:05 AM (1 year, 4 months ago) |
|
|
Quote:
Wildcat1883 said: These look like PEU to yβall?

They do to me
|
ChildOfTheMoon
Stranger


Registered: 01/07/22
Posts: 143
Last seen: 10 months, 16 days
|
Re: Cultivation General Discussion [Re: Grind365] 3
#27953892 - 09/17/22 01:12 AM (1 year, 4 months ago) |
|
|
Personally I donβt measure the temp of the agar before cooling. I just leave the jars in the pot until theyβre comfortable to touch, then transfer them to the SAB to pour.
|
Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
Last seen: 10 hours, 48 minutes
|
|
Quote:
ChildOfTheMoon said: Personally I donβt measure the temp of the agar before cooling. I just leave the jars in the pot until theyβre comfortable to touch, then transfer them to the SAB to pour.
Yeah same here. No measuring. I'll admit that for pouring (in sab) with 0 condensation there's a very narrow window of opportunity to get it just right. Which is pour right before the agar mix wants to solidify.
That's why I will usually end up with some condensation on the top plates of each stack. Putting a glass of warm water on top of your stacks right after the pour helps a little bit in terms of more even cooldown and less condensation on the lids.
|
SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,907
Loc: Rent free in your head
Last seen: 4 hours, 27 minutes
|
Re: Cultivation General Discussion [Re: Grind365] 3
#27953988 - 09/17/22 04:56 AM (1 year, 4 months ago) |
|
|
If you have proper sterile technique there aren't any contams for the water to move around.
Don't blame the water, you introduced the contams
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
π
π
π
£π
£π
π
π
π
π
π
    "Mist your balls and fan your asshole" - Pandaskis, 2023
|
Brian Jones
Club 27



Registered: 12/18/12
Posts: 12,342
Loc: attending Snake Church
Last seen: 16 hours, 59 minutes
|
Re: Cultivation General Discussion [Re: Hobbit GDF] 1
#27954056 - 09/17/22 06:58 AM (1 year, 4 months ago) |
|
|
Quote:
Hobbit GDF said: Gotta have a pressure cooker. My must haves are pressure cooker & flowhood.
It changes the game completely.
Gotta have a pressure cooker. It's not hard to work up to 95% success with a SAB.
-------------------- "The Rolling Stones will break up over Brian Jones' dead body" John Lennon I don't want no commies in my car. No Christians either. The worst thing about corruption is that it works so well,
|
nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 9 days, 21 hours
|
|
Quote:
ChildOfTheMoon said: Personally I donβt measure the temp of the agar before cooling. I just leave the jars in the pot until theyβre comfortable to touch, then transfer them to the SAB to pour.
I don't measure it either.
Instead of using one big jar for it I use a bunch of smaller jars, 250 ml media jars and sometimes a wide mouth half-quart mason jar.
For PCing media jars I have the lid a little lose, not tightened all the way. For the pint mason jars, I put a lid on finger tight, but it's a grow lid with a synthetic filter, that allows pressure to equalize and not blow up.
I let the PC shoot steam 10 min, then put on the stopcock with tongs, let it get to between 15 and 20 PSI for 22 minutes.
Then I let it cool on the stove to zero PSI reading, and bring the whole PC into the room with my flowhood, set the PC on some cardboard so it doesn't melt anything.
I open it and take one jar of agar out, and put the lid back on the PC, not tight, just sitting on top.
I pick up the agar jars wearing gloves and using some folded over toilet paper or paper towel to pick it up by the neck.
I pour that into plates in front of the flowhood until that bottle or mason jar is empty done. Then I put the bottle or jar back in the PC and grab another one.
Keeping them in the PC keeps them very warm to pour. Putting them back in the PC after pouring keeps the agar from solidifying and makes them easier to clean when done.
When done with all, I leave the plates covered cooling in stacks in front of the flow hood. I bring the PC back into the kitchen. I rinse out each jar or media bottle while still warm so the agar doesn't solidify and become hard to clean.
Then I put each jar in the dishwasher.
-------------------- -NEW? Start here.
|
Grind365
AlwaysGrinding

Registered: 08/07/22
Posts: 448
Last seen: 1 year, 6 days
|
Re: Cultivation General Discussion [Re: SirPsycho]
#27954532 - 09/17/22 12:33 PM (1 year, 4 months ago) |
|
|
Quote:
SirPsycho said: If you have proper sterile technique there aren't any contams for the water to move around.
Don't blame the water, you introduced the contams
Agreed. No one will argue this but my point being that it's ok to have contams on agar because we just transfer away from them taking only clean mycelium but water on the plate will slosh the contams around on the plate making it very hard to transfer away only clean mycelium. This can be very problematic if you are having contamination issues or starting from dirty spores. I didn't mean to try and invalidate your point at all.
|
YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,209
Loc: Castles made of sand
Last seen: 1 hour, 30 minutes
|
Re: Cultivation General Discussion [Re: Grind365] 4
#27954576 - 09/17/22 01:12 PM (1 year, 4 months ago) |
|
|
I no prep in jars and just dump any liquid right before making transfers. I also flip my jars the day after taking transfers. I'll often keep the liquid if I'm starting spores. Once, I even dumped some of the liquid into a mini zip top bag that had smooshed agar. It made a mini LI that in turn, nocced a few jars of agar.
|
SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
|
Re: Cultivation General Discussion [Re: YoshiTrainer] 1
#27954685 - 09/17/22 02:24 PM (1 year, 4 months ago) |
|
|
Yeah the water is very useful for germ plates. Saving the water for other shit is pretty resourceful though, nice idea!
|
cozmyc
gentle modern ape



Registered: 06/20/21
Posts: 2,131
Last seen: 19 hours, 9 minutes
|
Re: Cultivation General Discussion [Re: SwabMarley]
#27954841 - 09/17/22 03:52 PM (1 year, 4 months ago) |
|
|
Quote:
Myc Hunt said: Yeah the water is very useful for germ plates. Saving the water for other shit is pretty resourceful though, nice idea!
Yup, Just fed my plants some grain water!
-------------------- You're conscious population 2 stardust ---------------------- and that's valuable
|
Jaksavage
Mycodelic



Registered: 11/19/21
Posts: 548
Loc: Left coast
Last seen: 6 months, 4 hours
|
Re: Cultivation General Discussion [Re: wild_aussie] 2
#27954950 - 09/17/22 04:56 PM (1 year, 4 months ago) |
|
|
I was just thinking of making a poll to gather data on germination failure %. Basically what is the waste rate per each style of germination. MSS, LI, LC, myc, g2a, cloning.
Then I got distracted...
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
Last seen: 3 hours, 7 minutes
|
Re: Cultivation General Discussion [Re: Inocuole] 2
#27954961 - 09/17/22 05:01 PM (1 year, 4 months ago) |
|
|
If anyone is interested, Nichromeβs hosting a little contest
https://www.shroomery.org/forums/showflat.php/Number/27954943
|
nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 9 days, 21 hours
|
Re: Cultivation General Discussion [Re: Jaksavage]
#27955287 - 09/17/22 08:48 PM (1 year, 4 months ago) |
|
|
Quote:
Jaksavage said: I was just thinking of making a poll to gather data on germination failure %. Basically what is the waste rate per each style of germination. MSS, LI, LC, myc, g2a, cloning.
I mostly do clones, usually the same clone, and they're pretty close to zero failure.
-------------------- -NEW? Start here.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
Last seen: 3 hours, 7 minutes
|
Re: Cultivation General Discussion [Re: Inocuole]
#27955292 - 09/17/22 08:50 PM (1 year, 4 months ago) |
|
|
Once I figure out best practices for taking tissue samples cleanly, Iβm going to lean hard into cloning. Spore streaks are extremely reliable for me as well.
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 1 day, 19 hours
|
Re: Cultivation General Discussion [Re: Smellyhobbit] 3
#27955309 - 09/17/22 08:59 PM (1 year, 4 months ago) |
|
|
split the stipe down the center & take tissue from the inner part of the mushroom for cloning. nothing fancy. the inner tissue is inherently sterile, baring any fuck ups in your techniques. pretty much the gold standard way to clone anything
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
Last seen: 3 hours, 7 minutes
|
Re: Cultivation General Discussion [Re: Inocuole]
#27955312 - 09/17/22 09:01 PM (1 year, 4 months ago) |
|
|
Thatβs what Iβve tried to do, but I definitely had bad technique. I mean a few attempts ago I was peeling the tissue out with a gloved hand which.. I mean obviously that was bacterial. I actually think I got it figured out with this last clone.
Split the stipe, then carved out a piece with a scalpel, only touching the inside of the fruit body. Tomorrow weβll see how it grows out. Itβs about time to see growth. No bacteria visible so far, so thatβs good.
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 1 day, 19 hours
|
Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#27955331 - 09/17/22 09:16 PM (1 year, 4 months ago) |
|
|
lmao yeah you canβt just grab it out with your hands, gotta use the scalpel in an SAB & shit. i usually tear the mushroom with my bare hands but any tissue intended for use in cloning needs to be handled like you would a wedge of agar for transfer. sounds like you did it right this time, good luck!
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
|
YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,209
Loc: Castles made of sand
Last seen: 1 hour, 30 minutes
|
Re: Cultivation General Discussion [Re: hazyhorse] 2
#27955422 - 09/17/22 10:17 PM (1 year, 4 months ago) |
|
|
Needle nose tweezers work really well!
|
Wildcat1883
Learning



Registered: 11/14/18
Posts: 86
Last seen: 1 year, 1 month
|
Re: Cultivation General Discussion [Re: YoshiTrainer] 1
#27955472 - 09/17/22 11:53 PM (1 year, 4 months ago) |
|
|
So I should prbly harvest some of these right? First time w PEU. Also should I take the spores from the clusters? And what is the best way to get spores from PEU?
--------------------
|
|