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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
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Re: Cultivation General Discussion [Re: Inocuole]
#27942014 - 09/09/22 01:47 PM (1 year, 4 months ago) |
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Quote:
Smellyhobbit said:
Quote:
Nerex said: I'm gonna try my 3rd cultivation ever and I've choose the following way: LC + popcorn grains with no PC in shoeboxes. My doubt is: it's enough to spawn the grain in just plain coco coir (as substrate) in the shoebox? Will that be enough or do I need also vermiculite and gypsum? I'd gladly avoid also having to find these last 2, are they necessary?
You’re not going to sterilize your grains? I’m not sure you’ll see much success that way, to be honest.
Yeah straight coir is a fine performer. Vermiculite is good to have to adjust moisture content, but if you get field capacity right in your coir then you don’t need verm. Gypsum isn’t really used much anymore, so don’t worry about that.
I think popcorn can be done without a PC.. you do a steam sterilization similar to PF Tek without a PC
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smalltalk_canceled
Babnik



Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 11 hours
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Re: Cultivation General Discussion [Re: Nerex] 1
#27942085 - 09/09/22 02:41 PM (1 year, 4 months ago) |
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Quote:
Nerex said: I'm gonna try my 3rd cultivation ever and I've choose the following way: LC + popcorn grains with no PC in shoeboxes. My doubt is: it's enough to spawn the grain in just plain coco coir (as substrate) in the shoebox? Will that be enough or do I need also vermiculite and gypsum? I'd gladly avoid also having to find these last 2, are they necessary?
I feel so guilty having trained so many guys in corn. Oats may contam weirdly but it's better nutrition.
Anyways, focus on good grain prep (dry exterior) and good coir field capacity (no drops of water when coir is squeezed)
use the vermiculite to make the mix airy. Coarse verm is best imo.
No visible grains on top.
You can drop the lime/gypsum for cubes, i dont think anyone can verify that it matters
Good luck cornist, I'm oats now
Also, buy a pressure cooker. You need one of those
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (09/09/22 02:44 PM)
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station
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I use calcium sulfate & calcium carbonate at different times and I do know the way I use them seems to help. Those are things that go into my mixes and I will continue to use them.
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Nerex
Stranger

Registered: 02/06/22
Posts: 176
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Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#27942268 - 09/09/22 04:43 PM (1 year, 4 months ago) |
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Quote:
Smellyhobbit said:
Quote:
Nerex said: I'm gonna try my 3rd cultivation ever and I've choose the following way: LC + popcorn grains with no PC in shoeboxes. My doubt is: it's enough to spawn the grain in just plain coco coir (as substrate) in the shoebox? Will that be enough or do I need also vermiculite and gypsum? I'd gladly avoid also having to find these last 2, are they necessary?
You’re not going to sterilize your grains? I’m not sure you’ll see much success that way, to be honest.
Yeah straight coir is a fine performer. Vermiculite is good to have to adjust moisture content, but if you get field capacity right in your coir then you don’t need verm. Gypsum isn’t really used much anymore, so don’t worry about that.
Oh yes of course I will, with the pot method, like I did with the BRF cakes. I discovered just few days ago that I'll be able to sterilize that grain without the PC which I don't have. My doubt was about the nutrients (some say that popcorn doesn't have many, but I don't know if that's true or not). I want to keep it simple: LC for fast colonization and coco coir as fruiting substrate with a layer on top. : )
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smalltalk_canceled
Babnik



Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 11 hours
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Re: Cultivation General Discussion [Re: Hobbit GDF]
#27942289 - 09/09/22 04:53 PM (1 year, 4 months ago) |
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Quote:
Hobbit GDF said: I use calcium sulfate & calcium carbonate at different times and I do know the way I use them seems to help. Those are things that go into my mixes and I will continue to use them.
Okay, im open to this, just been influenced by the hard hate for this in the woodlover thread.(?)
I don't see much active threads lately discussing lime and pH but I'm sure it's part of a lot of teks and the knowledge of some. Haven't seen it much
In my experience growing a few lil psilocybes and taking them to coir, they only tolerate slight amounts otherwise HARD dislike
Compare to CV, CVG is kinda dead
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (09/09/22 04:53 PM)
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station
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Here are 4 malmac bags. Starting to pin.
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Rabbit84
Ate Shroomz


Registered: 08/04/22
Posts: 160
Last seen: 9 months, 3 days
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Re: Cultivation General Discussion [Re: Hobbit GDF] 1
#27942585 - 09/09/22 08:03 PM (1 year, 4 months ago) |
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Quote:
Hobbit GDF said: Here are 4 malmac bags. Starting to pin.

They're beautiful ❤️
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Rabbit84
Ate Shroomz


Registered: 08/04/22
Posts: 160
Last seen: 9 months, 3 days
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Re: Cultivation General Discussion [Re: Nerex]
#27942601 - 09/09/22 08:14 PM (1 year, 4 months ago) |
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Quote:
Nerex said:
Quote:
Smellyhobbit said:
Quote:
Nerex said: I'm gonna try my 3rd cultivation ever and I've choose the following way: LC + popcorn grains with no PC in shoeboxes. My doubt is: it's enough to spawn the grain in just plain coco coir (as substrate) in the shoebox? Will that be enough or do I need also vermiculite and gypsum? I'd gladly avoid also having to find these last 2, are they necessary?
You’re not going to sterilize your grains? I’m not sure you’ll see much success that way, to be honest.
Yeah straight coir is a fine performer. Vermiculite is good to have to adjust moisture content, but if you get field capacity right in your coir then you don’t need verm. Gypsum isn’t really used much anymore, so don’t worry about that.
Oh yes of course I will, with the pot method, like I did with the BRF cakes. I discovered just few days ago that I'll be able to sterilize that grain without the PC which I don't have. My doubt was about the nutrients (some say that popcorn doesn't have many, but I don't know if that's true or not). I want to keep it simple: LC for fast colonization and coco coir as fruiting substrate with a layer on top. : )
I don't know much, haven't even gotten my first batch of rye berries fully colonated yet, but I see how easily things can get contaminated and how important water content on grains is. I don't think I would want to risk waisting all the time that I put into this by cutting corners on these extremely important parts of the process. But again, I don't know really know anything
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: Rabbit84] 1
#27943037 - 09/10/22 05:10 AM (1 year, 4 months ago) |
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Well you’re right even though you’re doubting yourself. Homie needs to pc his grains or he’ll be looking at jars of green. Lc is fast but mold is faster
-------------------- Life’s shit, but I’m loving it
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Rabbit84
Ate Shroomz


Registered: 08/04/22
Posts: 160
Last seen: 9 months, 3 days
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Re: Cultivation General Discussion [Re: Inocuole]
#27943398 - 09/10/22 09:03 AM (1 year, 4 months ago) |
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Quote:
Wall.E said: Well you’re right even though you’re doubting yourself. Homie needs to pc his grains or he’ll be looking at jars of green. Lc is fast but mold is faster
Yeah, I had 2 out of my first batch of 20 agar plate get contaminated. Not bad for my first time. But then I used 2 plates that had absolutely no growth to attempt T1 transfers. And I reused the parafilm... So I ended up stretching it a little too much and tiny holes ripped between the top plate and bottom plate that I didn't notice. 3 days later, the whole plate is contaminated. I learned my lesson and how easily contamination can happen. They were even stored in another air tight container.
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Nerex
Stranger

Registered: 02/06/22
Posts: 176
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Re: Cultivation General Discussion [Re: Wall.E]
#27943416 - 09/10/22 09:20 AM (1 year, 4 months ago) |
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I was also thinking of start doing agar, so I many be using that (also) to be sure to have a clean inoculant to work with and also experiment a bit in the future. I'm still not willing to get a PC though, considering that I wouldn't cultivating really that much at all.
That said, with agar and "sterilizing" popcorn with the usual 90 minutes in the pot, do you still see THAT much of a risk of contamination compared to PCing the popcorns?
I was looking for other grain relatively safe to use without the PC, but it seems that there's only the brown rice other than the porcorns.
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station
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Re: Cultivation General Discussion [Re: Nerex]
#27943433 - 09/10/22 09:30 AM (1 year, 4 months ago) |
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Gotta have a pressure cooker. My must haves are pressure cooker & flowhood.
It changes the game completely.
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
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Re: Cultivation General Discussion [Re: Hobbit GDF]
#27943442 - 09/10/22 09:35 AM (1 year, 4 months ago) |
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Quote:
Hobbit GDF said: Gotta have a pressure cooker. My must haves are pressure cooker & flowhood.
It changes the game completely.
As much as I’d love the space and funds for a flow hood, I can’t have one right now. One day. Sure will make agar a more pleasant experience.
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new2u
Stranger

Registered: 07/09/22
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Last seen: 7 months, 10 days
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Re: Shoebox, to progress? [Re: Inocuole]
#27943462 - 09/10/22 09:49 AM (1 year, 4 months ago) |
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Hiya,
So, I saw some stuff on on here about shoebox/lunchbox, so decided to give it a go. This is were I'm at - got here with a lid on the tub, offset not clamped shut. I was wondering how best to meddle - or not. Should I put lid back on till they touching it? Should I start weeding some out?
Any advice appreciated.
XX
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station
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Re: Shoebox, to progress? [Re: new2u]
#27943502 - 09/10/22 10:27 AM (1 year, 4 months ago) |
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Leave lid on until they touching it or get another shoebox and put on top like a dome.
Don't need to "weed" any out.
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new2u
Stranger

Registered: 07/09/22
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Last seen: 7 months, 10 days
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Re: Shoebox, to progress? [Re: Hobbit GDF]
#27943542 - 09/10/22 10:59 AM (1 year, 4 months ago) |
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tyvm
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station
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Re: Shoebox, to progress? [Re: new2u] 3
#27943706 - 09/10/22 12:49 PM (1 year, 4 months ago) |
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These tp growing fast.

That's the biggest one in all 4 bags
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
Last seen: 2 hours, 37 minutes
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Re: Shoebox, to progress? [Re: Inocuole]
#27943992 - 09/10/22 04:17 PM (1 year, 4 months ago) |
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Learned I don’t have experience enough to make and use LC yet. Had a plate that looked clean, chopped it up, threw it in some broth. Growth looked great. Posted it here, got good feedback. Instead of testing it on a Petri, since I lost all my plates to contam, I just made a few quarts of millet and noc’d it up. Figure that’s as good as a plate if you’re just seeing if it’s contaminated.
Boy was it contaminated. All I grew was some sweet smelling green mold. This stuff looks identical to pictures of healthy LC here on shroomery, but it was totally worthless. I suppose my plate wasn’t as clean as I thought, but if it wasn’t then it really fooled me.
Anyway, I haven’t had back luck inoculating with agar to grain, so I’m just going to keep doing that. LC might save a little time, but if you do new grain every week it hardly matters.
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monkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 24 days
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Quote:
Smellyhobbit said: Learned I don’t have experience enough to make and use LC yet. Had a plate that looked clean, chopped it up, threw it in some broth. Growth looked great. Posted it here, got good feedback. Instead of testing it on a Petri, since I lost all my plates to contam, I just made a few quarts of millet and noc’d it up. Figure that’s as good as a plate if you’re just seeing if it’s contaminated.
Boy was it contaminated. All I grew was some sweet smelling green mold. This stuff looks identical to pictures of healthy LC here on shroomery, but it was totally worthless. I suppose my plate wasn’t as clean as I thought, but if it wasn’t then it really fooled me.
Anyway, I haven’t had back luck inoculating with agar to grain, so I’m just going to keep doing that. LC might save a little time, but if you do new grain every week it hardly matters.
iirc you don't use a FH? i didn't consider doing LC without one, josex poke could help you out tho sorry for the loss
Edited by monkey_accident (09/10/22 04:29 PM)
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
Last seen: 2 hours, 37 minutes
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Quote:
monkey_accident said:
Quote:
Smellyhobbit said: Learned I don’t have experience enough to make and use LC yet. Had a plate that looked clean, chopped it up, threw it in some broth. Growth looked great. Posted it here, got good feedback. Instead of testing it on a Petri, since I lost all my plates to contam, I just made a few quarts of millet and noc’d it up. Figure that’s as good as a plate if you’re just seeing if it’s contaminated.
Boy was it contaminated. All I grew was some sweet smelling green mold. This stuff looks identical to pictures of healthy LC here on shroomery, but it was totally worthless. I suppose my plate wasn’t as clean as I thought, but if it wasn’t then it really fooled me.
Anyway, I haven’t had back luck inoculating with agar to grain, so I’m just going to keep doing that. LC might save a little time, but if you do new grain every week it hardly matters.
iirc you don't use a FH? honestly not recommended doing LC without one, josex poke could help you out tho sorry for the loss 
It’s ok. Maybe that’s really the answer, waiting until I have a solid lab. I find it, so far, pretty easy to clean agar under a SAB as long as you know you have good plates. I’ve cleaned up a handful in short order since getting my agar working.
I love the idea of liquid culture. But for now I’m very comfortable with not having to introduce several more vectors for contamination into an already clean workflow. A2G is perfectly serviceable.
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