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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
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Re: Cultivation General Discussion [Re: Inocuole] 1
#27924397 - 08/29/22 04:33 PM (1 year, 4 months ago) |
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So I was taking a look at the well known Muda Bottle Writeup and started thinking about starting some. I have a few going right now that I just spawned to bulk in to get a feel for how full they should be and what they’ll look like, but that’s not really the point. The point of the Muda bottle is to have everything ready to go out of the PC.
I’ve got a test bottle in the PC right now combining that with Crack’s No Prep Millet Tek to see if it’ll work. A cup of millet, a half cup of water, and eyeballed coir until there was enough room left for the millet to expand.
No idea if this is gonna work or if the millet will come out a pasty mess. We’ll see in a couple of hours.
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Guerrilla
Bumbaclart


Registered: 01/30/21 
Posts: 3,170
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: Smellyhobbit] 2
#27924476 - 08/29/22 05:28 PM (1 year, 4 months ago) |
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So 3/4 weeks ago I discovered that I'd left a brew bag of boiled rye grain that didn't get used in the corner of my room.
It was full of aspergillus, absolutely fucked amount.
Lifting it into the bin released a thick green cloud of spores across the room.
I've started seeing aspergillus in some jars that received G2G a week ago, despite using a LFH, and never having issues. I certainly hadn't seen aspergillus in jars before.
And I've just found it on a substrate that was finished up.
Now that's a problem.
Was supposed to spend tomorrow doing a shit ton of useful stuff, but now I'm deep cleaning the room and running a fucking ozone generator.
Not bothering switching the flow hood on until that's done. Unfortunate times.
I think the ozone generator alone would do the trick but I'm not feeling better about it until that whole room is emptied, and every single inch and every single thing that goes back in that room is cleaned.
Stupid laziness. I'd already stopped leaving grain in brew bags since then, but now it's definitely a lesson learned.
-------------------- Being pissed on does not make you a real man.
Edited by Guerrilla (08/29/22 05:31 PM)
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
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Re: Cultivation General Discussion [Re: Guerrilla]
#27924478 - 08/29/22 05:30 PM (1 year, 4 months ago) |
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Bro that’s extremely unfortunate. My condolences. So I take it this wasn’t PC’d grain? Man that’s like bedbugs levels of action required.
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Guerrilla
Bumbaclart


Registered: 01/30/21 
Posts: 3,170
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#27924485 - 08/29/22 05:38 PM (1 year, 4 months ago) |
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Honestly man been sitting here with my head in my hand. Super stressed but it should be fixable. I lost 6 out of 36 jars but I'm just glad I managed to recognize the mold and put two and two together.
Yup just boiled and not sterilized, I put the bag somewhere semi hidden and totally forgot about it so I didn't notice it for some time.
-------------------- Being pissed on does not make you a real man.
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
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Re: Cultivation General Discussion [Re: Inocuole] 1
#27924489 - 08/29/22 05:42 PM (1 year, 4 months ago) |
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Well at least it happened to someone smart enough to handle it, huh. It sucks real bad, but you’ll get it taken care of.
Is this going to impact the efficacy of the LFH later? Or since it’s basically made to handle that will it be ok?
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Guerrilla
Bumbaclart


Registered: 01/30/21 
Posts: 3,170
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#27924497 - 08/29/22 05:48 PM (1 year, 4 months ago) |
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It should be fine, I'll run it on high for a good while and the ozone generator should take care of the rest.
I'm just hoping the generator is as efficient as I need it to be. 20,000mg/HR. I'm gonna run it a few times at least. Might as well run it in every single room, it's pretty neat what they're capable of.
-------------------- Being pissed on does not make you a real man.
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Egon_Spengler
Ever lurkin never workin



Registered: 04/02/22
Posts: 2,294
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Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#27924701 - 08/29/22 07:56 PM (1 year, 4 months ago) |
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Quote:
Smellyhobbit said: So I was taking a look at the well known Muda Bottle Writeup and started thinking about starting some. I have a few going right now that I just spawned to bulk in to get a feel for how full they should be and what they’ll look like, but that’s not really the point. The point of the Muda bottle is to have everything ready to go out of the PC.
I’ve got a test bottle in the PC right now combining that with Crack’s No Prep Millet Tek to see if it’ll work. A cup of millet, a half cup of water, and eyeballed coir until there was enough room left for the millet to expand.
No idea if this is gonna work or if the millet will come out a pasty mess. We’ll see in a couple of hours.
Any updates on how the grains hydrated? I too have been playing with a couple bottle grows as bulk spawn vessels, but only because I had the colonized grain around in jars already, and am trying to keep individual test batches smaller than shoeboxes. I’d like to hear your thoughts on this first run. I’ve some MS natalensis cultures on agar that I’ll be ready to put to grain in a couple weeks, and I’m really leaning towards a full Muda method.
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
Last seen: 1 hour, 33 minutes
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Re: Cultivation General Discussion [Re: Inocuole] 1
#27924748 - 08/29/22 08:18 PM (1 year, 4 months ago) |
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Today I used some clean Avery’s culture on agar to make some LC to test my next round of Muda bottles. Here’s the preliminary results of this test bottle, which obviously won’t be used.


Sorry it’s so dirty. Had to shake it to mix ingredients before PCing. Which I may not do next time.
Anyway, if you can’t tell, the grains only expanded up to the 3 cup line, which isn’t bad but I think it could do with a bit more coir next time. I made them a little over halfway full. The coir doesn’t expand, which seems obvious now, but I wanted to make good and sure nothing went awry.
As for hydration, they’re still quite wet. Fully hydrated, but wet. Almost mushy, but still held together. I’m letting them sit overnight like I would with regular no prep millet to see if the moisture content reaches more of an equilibrium.
I’m thinking the moisture from the coir might be having an impact on the millet. I could do another tomorrow with less water to see how it performs. Muda bottles may just need soaked grains instead of dry, but I’m going to keep doing tests for a little while.
Also, not that it matters, but pressure cooked millet and coir smells an awful lot like barbecue.
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Rabbit84
Ate Shroomz


Registered: 08/04/22
Posts: 160
Last seen: 9 months, 3 days
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Re: Cultivation General Discussion [Re: Inocuole] 2
#27924749 - 08/29/22 08:19 PM (1 year, 4 months ago) |
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Do you guys think this lid upside down on this tub will work similar to Bods unmodified tub? I'd rather not drill holes. Not because I'm lazy, just thinks there's more room for error with the holes. Also, this will be my first tub so any advice you want to give will be appreciated.
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san pedro guy
Captain



Registered: 10/22/17
Posts: 1,806
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: Rabbit84] 1
#27924760 - 08/29/22 08:25 PM (1 year, 4 months ago) |
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Quote:
Rabbit84 said: Do you guys think this lid upside down on this tub will work similar to Bods unmodified tub? I'd rather not drill holes. Not because I'm lazy, just thinks there's more room for error with the holes. Also, this will be my first tub so any advice you want to give will be appreciated.

looks fine to me home skillet
-------------------- Noob Grow Along 2022
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,209
Loc: Castles made of sand
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Smellyhobbit, I've had better luck putting all the grains at the bottom of a jar when doing AIO. Maybe give it a try with one next round? I'll have to read Bod's TEK but for me millet is 2:1 by volume. If you put 2Tbsp millet and 1Tbsp water in the bottom then your COIR. I think COIR is about 80g:1C water.
Good luck!
I forgot, add a pinch of gypsum for the millet so you don't have to shake so hard.
Edited by YoshiTrainer (08/29/22 10:38 PM)
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Egon_Spengler
Ever lurkin never workin



Registered: 04/02/22
Posts: 2,294
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Re: Cultivation General Discussion [Re: YoshiTrainer] 2
#27925089 - 08/30/22 12:56 AM (1 year, 4 months ago) |
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Quote:
Smellyhobbit said: Here’s the preliminary results of this test bottle… .
Thanks for the report, Smelly. I hope letting it sit overnight will help redistribution of moisture for you. Appreciate the update.
Quote:
YoshiTrainer said: Smellyhobbit, I've had better luck putting all the grains at the bottom of a jar when doing AIO. Maybe give it a try with one next round? I'll have to read Bod's TEK but for me millet is 2:1 by volume. If you put 2Tbsp millet and 1Tbsp water in the bottom then your COIR. I think COIR is about 80g:1C water.
Yoshi, Thanks for sharing the tips. I know they were directed at Smelly, but I’m taking notes too and will test out this ratio next week.
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Rabbit84
Ate Shroomz


Registered: 08/04/22
Posts: 160
Last seen: 9 months, 3 days
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Quote:
san pedro guy said:
Quote:
Rabbit84 said: Do you guys think this lid upside down on this tub will work similar to Bods unmodified tub? I'd rather not drill holes. Not because I'm lazy, just thinks there's more room for error with the holes. Also, this will be my first tub so any advice you want to give will be appreciated.

looks fine to me home skillet
Is the unmodified tub what everyone is doing these days?
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,289
Loc: South Africa
Last seen: 27 seconds
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Re: Cultivation General Discussion [Re: Rabbit84] 1
#27925342 - 08/30/22 07:18 AM (1 year, 4 months ago) |
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Nope, we do all kinds I currently run shoeboxes, pasty easy dial tubs, un modified and old school monotubs They all work.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: Cultivation General Discussion [Re: DERRAYLD]
#27925364 - 08/30/22 07:38 AM (1 year, 4 months ago) |
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Anything will work. It’s all about dialing things in for your environment.
--------------------
LAGM2020     
Edited by A.k.a (08/30/22 07:39 AM)
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wild_aussie
Punk rocker til I die



Registered: 01/05/22
Posts: 1,000
Loc: Skatepark grinding hard
Last seen: 6 days, 2 hours
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Re: Cultivation General Discussion [Re: Rabbit84]
#27925438 - 08/30/22 08:47 AM (1 year, 4 months ago) |
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Quote:
Rabbit84 said:
Quote:
san pedro guy said:
Quote:
Rabbit84 said: Do you guys think this lid upside down on this tub will work similar to Bods unmodified tub? I'd rather not drill holes. Not because I'm lazy, just thinks there's more room for error with the holes. Also, this will be my first tub so any advice you want to give will be appreciated.

looks fine to me home skillet
Is the unmodified tub what everyone is doing these days?
No the one bod uses for unmod has four blue clamps on them but they all will work if you don't want to drill holes you can set the lid at a slight angle or use it as a water tub for brf cakes or a fruiting chamber for shoeboxes with cracked lid sir pyscho is growing in an AA book in a ziplock baggy(which also work as fruiting chambers)
-------------------- BEWARE OF PICK POCKETS AND LOOSE WOMEN!! "Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!" Hunter S. Thompson "I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse " Sticking feathers up your ass does not make you a chicken!" Tyler Durden "He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson The noob forum Crack Plates Fastfreds media cookbook 60 agar/lc recipes Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide Trusted teks and linksSEED "PETRI" TEK Vent ur PC
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station
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Re: Cultivation General Discussion [Re: wild_aussie] 2
#27925483 - 08/30/22 09:11 AM (1 year, 4 months ago) |
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Opened my melmac bags today. Here is the surface moisture

The other bags are the same. I opened the vent on 5 bags. 4 bags are about 5-10 lbs 1 bags is 10-20 lbs
They are mixed 1:1 - 1:2
2 more 10lb bags are a week behind the other 5.
All look good, no signs of baddies. Very clean.
Should I wait until there are pins before I open the vents? That's what I wonder. One bag has slight pooling of water so I might try and syringe it out. If I tip the bag it will mess up the almost perfect surface moisture. So I can't pour it out.
Edited by Hobbit GDF (08/30/22 10:02 AM)
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
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Last seen: 1 hour, 33 minutes
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Re: Cultivation General Discussion [Re: Inocuole] 2
#27926073 - 08/30/22 03:51 PM (1 year, 4 months ago) |
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So around 20-22 hours later, the moisture situation in the Muda/Crack bottle has settled and it’s pretty much exactly what we’re looking for. I’m going to do another tomorrow with all the millet at the bottom, give it a shake, and let it sit for 24 hours. I’ll get results for that one too this week, but I’ll say shaking it all up before cooking it actually wound up working great. It looked a little wet, but then again so does millet before it settles.
I’m gonna say once the LC is prepared it’s going to work great.
Edit: I dumped the grain/coir mixture into the trash and it all slid out in one clean go. Grains and coir moved loosely and didn’t stick together. Actually a really fantastic end result. I do wonder if the ratio of grain:sub is too high. Usually when we spawn, it’s 1:2, but this winds up being about 2:1. Should that be adjusted for?
Edited by Smellyhobbit (08/30/22 04:32 PM)
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Egon_Spengler
Ever lurkin never workin



Registered: 04/02/22
Posts: 2,294
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Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#27926228 - 08/30/22 05:06 PM (1 year, 4 months ago) |
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Quote:
Smellyhobbit said: So around 20-22 hours later, the moisture situation in the Muda/Crack bottle has settled and it’s pretty much exactly what we’re looking for. I’m going to do another tomorrow with all the millet at the bottom, give it a shake, and let it sit for 24 hours. I’ll get results for that one too this week, but I’ll say shaking it all up before cooking it actually wound up working great. It looked a little wet, but then again so does millet before it settles.
I’m gonna say once the LC is prepared it’s going to work great.
Edit: I dumped the grain/coir mixture into the trash and it all slid out in one clean go. Grains and coir moved loosely and didn’t stick together. Actually a really fantastic end result. I do wonder if the ratio of grain:sub is too high. Usually when we spawn, it’s 1:2, but this winds up being about 2:1. Should that be adjusted for?
Great update, happy to hear you found it successful.
2:1 certainly does seem high. I’d imagine it would be pretty easy to adjust for since the coir is already hydrated. Maybe start by cutting the grain/water by half, then maybe 2/3rds, and up the coir by anticipated volume…
…there’s always some new variable to test…good thing that’s the fun part
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
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Re: Cultivation General Discussion [Re: Inocuole] 2
#27927350 - 08/31/22 11:12 AM (1 year, 4 months ago) |
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Did you guys know that tomorrow starts "National Mushroom Month" for those of us in the US?
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