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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Josex]
#24195821 - 03/26/17 09:03 PM (6 years, 10 months ago) |
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Quote:
Josex said: We need to make a thread recording our sab sessions, I think it would be awesome and everybody could learn a few tricks here and there.
I'm a hands on person. I like to see it.
If I recorded myself, I'm sure you guys would be like, wtf is that shit nigger
-------------------- KSSS And PE WBS.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Josex]
#24195824 - 03/26/17 09:03 PM (6 years, 10 months ago) |
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Moab if you have incense lit one and put it in ur sab. Watch the smoke it should barely move from a straight line as you move around your sab commit those movements to memory. You may just be working too fast if I feel myself getting sloppy I do it to remind myself how much so little can do.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: van hatton]
#24195828 - 03/26/17 09:04 PM (6 years, 10 months ago) |
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Record one post it. It wouldn't hurt to see.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: van hatton]
#24195845 - 03/26/17 09:13 PM (6 years, 10 months ago) |
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What I'm about to say may sound stupid and maybe it would not work for everybody, but I really started to improve my SAB work after consciously paying attention and being aware of the sensations in my fingers, arms, shoulders and back, like really feeling all the muscle contractions, the tensions, etc... That in turn will relax you and you'll become aware of your movements and whether or not they are smooth or stiff.
Well, that was my experience at least, now my SAB sessions really feel like a meditation on steroids, the 'monkey mind' just shuts the fuck up.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Cultivation General Discussion [Re: Moabfighter]
#24195866 - 03/26/17 09:21 PM (6 years, 10 months ago) |
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Quote:
Moabfighter said:
Quote:
PinPornProducer said: Sab movements need to be slow n steady, can't be doing Mr roboto in the thing. Still have to make sure your arms or hands are not over open jars. Can't clink jars together when g2ging. Make sure no fans are running including the furnace that would cause air currents in the work area.
Gonna focus more on this post when I work next.
Clinking jars together, the occasional getting flustered with my hands and maybe make a half pass over a jar before catching myself.
also my SAB is directly on the counter to the left of my fridge, which always seems to be doing something that makes noise.....
Need to tighten my SAB work up I think. I hope. Failure sucks. Continuing to fail after trying hard not to keep failing, sucks even more.
I do all my sab work on my coffee table. Another thing is, make sure the sab is in the exact working distance. You don't want to have to slide the box at all after you let it sit for 10 minutes to let the air settle.
I also prefer a flame port rather than pulling my hand out the sab to flame the scalpel everytime. I just set the torch outside the box a few inches away from the hole and just stick the scalpel through the hole into the flame
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Interesting setup, PPP.
Based off the coffee table alone, you seem like a real G. Also, is that a "glovebox"?
My box has two gaping holes, and it's upside down with a towel sealing the lid tight.
-------------------- KSSS And PE WBS.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Cultivation General Discussion [Re: Moabfighter]
#24195884 - 03/26/17 09:30 PM (6 years, 10 months ago) |
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That's an old pic, I cut the gloves off like 9 months ago
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Here's what I got. Top photo is the remaining Texas YC.
Other two of the three jars are a PE clone that I got from a friend on here. I made one transfer to master plates, let those grow, and inoculated. Whole plate in each jar.
Do those look great?
Here's a possible idea. You guys remember that shitty PC I had? Well the weight feels extremely light. Extremely lighter than a real 15 weight, far lighter than a 10.
I cook my pasty plates for 30-40 mins. Is it possible my agar plates prior to the new PC possibly are growing mold?
I'm grasping for straws at this point. The inside of my SAB has bleach, soap, water, I wear gloves that I soak and my arms with alcohol between every jar (possible vector)
Get this. I clean arms, go In box, clean plates and jars with ISO wipe, open plate, upside down, set plate on the lid, not sealed shut. Loosen jar lid. Exit box. I flame scalpel, arms out of box. Set red hot scalpel on top of box, grab alcohol, hurry and wipe my arms, grab scalpel with my soaked ISO gloves, go in box, lube scalpel handle with my left soaked ISO glove, wipe Jar lid one last time, lift plate from lid, cut my wedge, slowly remove from plate, set plate back on lid, slowly move agar wedge 6-8 inches away to the jar, open lid with right right hand, away from the agar,so my hand opening the lid never goes over it, and as neat and easy I can plop the wedge in, out lid back on.
Rinse and repeat.
-------------------- KSSS And PE WBS.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Cultivation General Discussion [Re: Moabfighter]
#24195998 - 03/26/17 10:31 PM (6 years, 10 months ago) |
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Do you wet paper towel down to place the jars on in the sab?
After you wipe everything down in the sab, do you mist the walls and lid with soapy water and let the air settle for 10 minutes before going and doing work?
I wouldn't rest the scalpel on anything after its sterilized, flame it and go right back into the sab and go to work. I also just wipe my scalpel handle down once with bleach water before the entire session and rest in on top of a bleach water soaked paper towel sitting over a half pint jar after every agar drop just to tighten lids up and move jars around. Other than that the scalpel is in my hand the entire time
No need to wipe your arms and hands down everytime imo. I do it once everytime I load new work into the sab and that's it
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Paperbagboy
Friend

Registered: 03/24/17
Posts: 163
Loc: The U.S.
Last seen: 6 years, 9 months
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Re: Cultivation General Discussion [Re: Josex]
#24196049 - 03/26/17 11:01 PM (6 years, 10 months ago) |
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Quote:
Josex said: What I'm about to say may sound stupid and maybe it would not work for everybody, but I really started to improve my SAB work after consciously paying attention and being aware of the sensations in my fingers, arms, shoulders and back, like really feeling all the muscle contractions, the tensions, etc... That in turn will relax you and you'll become aware of your movements and whether or not they are smooth or stiff.
Well, that was my experience at least, now my SAB sessions really feel like a meditation on steroids, the 'monkey mind' just shuts the fuck up.
Sometimes surgeons get serious problems too standing still so much.
Maybe try some accupuncture or tea to ease yourself into the Operation.
Thats just my two cents.
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Wicked Burn


Registered: 05/15/16
Posts: 254
Last seen: 7 days, 9 hours
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Re: Cultivation General Discussion [Re: van hatton]
#24196085 - 03/26/17 11:34 PM (6 years, 10 months ago) |
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Quote:
van hatton said: Moab if you have incense lit one and put it in ur sab. Watch the smoke it should barely move from a straight line as you move around your sab commit those movements to memory. You may just be working too fast if I feel myself getting sloppy I do it to remind myself how much so little can do.
I like this idea. Been doing pretty well with my SAB/sterile technique for a bit, but I'm going to do this anyway. Seems like something that should be done very early in the hobby to get a good visual of exactly what kind of air currents your arm/hand movements are making. Thanks for this tip van hatton!
--------------------
Mr. Pink: "Hey, why am I Mr. Pink?" Joe: "Because you're a faggot." -Reservoir Dogs
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Wicked Burn]
#24196094 - 03/26/17 11:39 PM (6 years, 10 months ago) |
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practice makes perfect!
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24196114 - 03/26/17 11:52 PM (6 years, 10 months ago) |
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Moab, at this point you could use a confidence booster. Something to make you feel better. I suggest a few cakes, inoculated by syringe. You should use 1/2 pints with lids with a GE filter and a ship. You should only encounter mold if it was in the syringe or the jar was not sterile. Now the MS syringe will probably have some bacteria BUT mycelium can overcome some bacteria. I am fairly confident you would grow some shrooms and it would at least be good for your confidence, and easing your mind a bit. Cakes are some what forgiving. Some sort of success should help reignite your passion some. Don't give up.
-------------------- JOIN THE POW WOW
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Moab this was advice given to me about my situation about 10 days ago from kinetic. I went from mostly contamed to clean plates and jars since so far again thanks kinetic
: Lose the long sleeve work shirt. You're only pushing air through the sleeves and potentially into the petris every time you move.
3: Try torching the blade and then some. You don't need to get the handle part red hot, just flame it for a second or two and make sure you get all sides. I don't know what you use but I use a propane torch with a push-button starter (important imo) and have the flame directed away from me and the sab. The flame is on very low. I keep the torch a foot and a half away from my sab as well.
4: I always iso my hands before I flame the scalpel (seems contradictory, keep reading). I just pour some in my hands and rub it around then I wait for about 30 seconds to let it dry for the most part before I flame and when I flame the scalpel I make sure the scalpel is tilted upward, higher than my hand. I also hold the scalpel at the very edge of the handle while flaming. I should mention that before I turn the torch on I already have an iso soaked paper towel in my sab. After I turn the torch on I rub my hands with iso but then after that I just wipe my hands with the paper towel instead for the rest of the session. I wipe my hand down every two or three dishes
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 21 days
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Re: Cultivation General Discussion [Re: van hatton]
#24196234 - 03/27/17 03:30 AM (6 years, 9 months ago) |
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Wonder how many monos you could get going at a time with one of these autoclaves
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Mattisfat]
#24196360 - 03/27/17 08:48 AM (6 years, 9 months ago) |
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I don't get why people dont leave their torch on the whole sab session
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: bodhisatta]
#24196374 - 03/27/17 08:55 AM (6 years, 9 months ago) |
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when I run my torch super low it makes the tip red-hot. Just looks sketchy
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: van hatton]
#24196380 - 03/27/17 08:57 AM (6 years, 9 months ago) |
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Way i see it there's nothing clean about re starting a torch during a sab session

Little cheap 15-20 dollar torch.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Cultivation General Discussion [Re: bodhisatta]
#24196394 - 03/27/17 09:02 AM (6 years, 9 months ago) |
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$20 and I love it
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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12 bucks I already had propane..
I mean yeah it wouldn't be clean but neither are we that's why we don't have hands over open media.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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