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nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 9 days, 19 hours
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Re: mycelium transfer [Re: Salty Dog]
#27852882 - 07/06/22 10:18 PM (1 year, 6 months ago) |
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Quote:
Salty Dog said: This may be a dumb question. Can I transfer grain spawn to additional jars and expand my spawn?
Yes. Search G2G or Grain to Grain
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Rotnpins
๐คฎ Rotten-Pins ๐



Registered: 01/11/22
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Loc: in (front of) the hood
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Re: mycelium transfer [Re: Salty Dog]
#27852883 - 07/06/22 10:18 PM (1 year, 6 months ago) |
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Quote:
Salty Dog said: This may be a dumb question. Can I transfer grain spawn to additional jars and expand my spawn?
Are you talking about doing a g2g (grain 2 grain) transfer like This? It's an old post, but you get the idea
Edit: nektar61 beat me to it by about 4 seconds
Edited by Rotnpins (07/06/22 10:19 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Quote:
spirit_shadow said:
Quote:
mushboy said:
Quote:
spirit_shadow said: You can take sterile water in a syringe and take contam free myc in a SAB and suck said myc into syringe. I used to do that a lot back when I first messed with pasty plates 
Sucking myc? Youโd be sucking in air as well. Dirty air.
Pastyplates didn't have dirty air via the one drop method in a very sterile SAB :p (once I got clean myc from a drop, obviously hit or miss getting clean myc per plate)

You can do a biopsy/josex style poke but sab are not sterile thatโs a good way to grow mold. Stab not suck..
Edited by mushboy (07/07/22 05:17 AM)
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: Cultivation General Discussion [Re: mushboy] 2
#27853217 - 07/07/22 07:01 AM (1 year, 6 months ago) |
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Quote:
mushboy said: Stab not suck..
The Lorena Bobbitt Story.... In theaters near you.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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Auron
Apologetic noob


Registered: 06/01/22
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Re: mycelium transfer [Re: SirPsycho]
#27853220 - 07/07/22 07:02 AM (1 year, 6 months ago) |
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Unless I couldnโt see them, are there any holes in Munichโs 64q tub?
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Rotnpins
๐คฎ Rotten-Pins ๐



Registered: 01/11/22
Posts: 4,738
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Re: Cultivation General Discussion [Re: SirPsycho]
#27853223 - 07/07/22 07:06 AM (1 year, 6 months ago) |
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Quote:
SirPsycho said:
Quote:
mushboy said: Stab not suck..
The Lorena Bobbitt Story.... In theaters near you.
omg, that made me laugh and I had just taken a drink. I almost had juice come out my nose.
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spirit_shadow
Feature not a bug



Registered: 08/15/11
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Re: Cultivation General Discussion [Re: mushboy]
#27853385 - 07/07/22 10:02 AM (1 year, 6 months ago) |
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Quote:
mushboy said:
Quote:
spirit_shadow said:
Quote:
mushboy said:
Quote:
spirit_shadow said: You can take sterile water in a syringe and take contam free myc in a SAB and suck said myc into syringe. I used to do that a lot back when I first messed with pasty plates 
Sucking myc? Youโd be sucking in air as well. Dirty air.
Pastyplates didn't have dirty air via the one drop method in a very sterile SAB :p (once I got clean myc from a drop, obviously hit or miss getting clean myc per plate)

You can do a biopsy/josex style poke but sab are not sterile thatโs a good way to grow mold. Stab not suck..
Way back when I first started pastyplates I did it quite a bit with good success, guess I got my SAB good enough :p
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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Nimpo
Big Black


Registered: 05/10/12
Posts: 2,375
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How long do you guys wait for a agar culture thatโs been in the fridge for a few months to warm up and be ready for transfers and sending to grain?
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san pedro guy
Captain



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Re: Cultivation General Discussion [Re: Nimpo]
#27853982 - 07/07/22 07:41 PM (1 year, 6 months ago) |
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Quote:
Nimpo said: How long do you guys wait for a agar culture thatโs been in the fridge for a few months to warm up and be ready for transfers and sending to grain?
0
-------------------- Noob Grow Along 2022
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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i would always take a transfer and grow a new culture so the time it takes for that.
ive been burned putting stored cultures straight to grains. fridges are really dirty places.
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Dj_karma_magnet


Registered: 04/03/19
Posts: 203
Last seen: 4 months, 6 days
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Re: Cultivation General Discussion [Re: mushboy]
#27854155 - 07/07/22 11:04 PM (1 year, 6 months ago) |
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Whatโs the hands down best substrate for rye berries in a monotub (ezdialed)? I have access to horse shit in large quantities.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Coir
-------------------- Lifeโs shit, but Iโm loving it
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high_desert
Pipe and Paper Afternoon



Registered: 10/25/16
Posts: 319
Loc: Hidden Study
Last seen: 11 months, 6 days
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Re: Cultivation General Discussion [Re: Wall.E]
#27854207 - 07/08/22 12:19 AM (1 year, 6 months ago) |
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What's the longest you guys have ever run a dehydrator for? The timing my flushes rn are making me think I might end up having mine running for four days straight.. It's just a nothing special Amazon cheap brand.. Hope it can take some punishment.
-------------------- Great threads for first grows and beyondโฌ๏ธ PF Tek Quick Coir/Verm Substrate PF Cakes as Spawn Fahtlocks!(Ziplock tek) Shoeboxes What senses do we lack that we cannot see or hear another world all around us?
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Crackatoa
Stranger in a strange land



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Re: Cultivation General Discussion [Re: high_desert] 2
#27854211 - 07/08/22 12:29 AM (1 year, 6 months ago) |
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Weeks straight
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Cultivation General Discussion [Re: Crackatoa] 1
#27854214 - 07/08/22 12:33 AM (1 year, 6 months ago) |
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 Literally nonstop
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high_desert
Pipe and Paper Afternoon



Registered: 10/25/16
Posts: 319
Loc: Hidden Study
Last seen: 11 months, 6 days
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Haha thanks
-------------------- Great threads for first grows and beyondโฌ๏ธ PF Tek Quick Coir/Verm Substrate PF Cakes as Spawn Fahtlocks!(Ziplock tek) Shoeboxes What senses do we lack that we cannot see or hear another world all around us?
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nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
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Re: Cultivation General Discussion [Re: Nimpo]
#27854225 - 07/08/22 01:03 AM (1 year, 6 months ago) |
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Quote:
Nimpo said: How long do you guys wait for a agar culture thatโs been in the fridge for a few months to warm up and be ready for transfers and sending to grain?
Straight from fridge to room temp agar plate. Grew the Z-Strain I posted last week like that, worked fine. Can just warm up on the plate.
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Auron
Apologetic noob


Registered: 06/01/22
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Re: Cultivation General Discussion [Re: nektar61]
#27854383 - 07/08/22 06:53 AM (1 year, 6 months ago) |
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So yes, never flame inside a sab. But what if youโre doing it inside a small bathroom and only using soapy water. Thereโs no fumes to ignite that way. Correct?
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: Cultivation General Discussion [Re: Auron] 1
#27854385 - 07/08/22 06:56 AM (1 year, 6 months ago) |
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It's not the fumes that's the problem with flame inside the SAB. Heat and especially open flame cause air movement... I'll let you figure out why that's not a good thing in a STILL air box.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
๐
๐
๐
ฃ๐
ฃ๐
๐
๐
๐
๐
๐
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Auron] 3
#27854386 - 07/08/22 06:57 AM (1 year, 6 months ago) |
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Heat makes turbulence last thing you want is a torch/lighter inside the sab.
Flame outside itโs not a big deal donโt know why that comes up.
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