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Offlinewild_aussie
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Re: Cultivation General Discussion [Re: Inocuole]
    #27851338 - 07/05/22 11:01 PM (1 year, 6 months ago)

So I'm getting ready to prep oats.  I want to use gypsum this time.  Do I add the gypsum to the jar after putting the prepped oats in and right before pop into the PC? Thanks.  Oh ya water is coming to boil and jars are in PC sterilizing as you read. Thanks


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BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
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Offlinenektar61S
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Re: Cultivation General Discussion [Re: wild_aussie]
    #27851355 - 07/05/22 11:24 PM (1 year, 6 months ago)

Quote:

wild_aussie said:
So I'm getting ready to prep oats.  I want to use gypsum this time.  Do I add the gypsum to the jar after putting the prepped oats in and right before pop into the PC? Thanks.  Oh ya water is coming to boil and jars are in PC sterilizing as you read. Thanks




Gypsum isn't needed. Most Trusted Cultivators and non-TCs with fat tubs of shrooms on here don't use it.

I did a poll. Read the comments, don't just look at poll results:
https://www.shroomery.org/forums/showflat.php/Number/26954400/fpart/all/vc/1

It's old tek adopted from growing plants. Not needed. It may have some use in PF tek in getting the cake out of jars whole easier, but you're not doing that you're doing bulk tek, right?

The MOST anyone can say is "It probably won't do any harm." And no one can say that with authority, no one's done tests with a clone and a control group.

Fat canopy tubs are authority here. Most people with fat canopy tubs don't use gypsum.

Don't use coffee either. Even older wrong non-tek.

Read this:
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=27646632&page=0&vc=1#27646632


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Edited by nektar61 (07/05/22 11:31 PM)


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Offlinewild_aussie
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Re: Cultivation General Discussion [Re: Inocuole]
    #27851357 - 07/05/22 11:28 PM (1 year, 6 months ago)

Thank you one more question.  I've read after people have prepped oats they put in fridge overnight I'm getting ready to fill jars and PC can/should I put in fridge until tomorrow when ready to put agar to them or just let them sit on counter until tomorrow afternoon thanks


--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC


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Offlinenektar61S
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Re: Cultivation General Discussion [Re: wild_aussie]
    #27851359 - 07/05/22 11:36 PM (1 year, 6 months ago)

Quote:

wild_aussie said:
Thank you one more question.  I've read after people have prepped oats they put in fridge overnight I'm getting ready to fill jars and PC can/should I put in fridge until tomorrow when ready to put agar to them or just let them sit on counter until tomorrow afternoon thanks





I don't do oats but any grain, if you have to wait to noc it, I'd put in the fridge. At room temp anything in there may start growing, however little.

Fridge temp slows growth.

Shroom agar noc (inoculation) in the jar starts out-competing any little amount of thing that may be in there.

We shoot for sterile, but someone on here said there is no true sterility, all of this hobby is a matter of minimizing contam and out-competing it with what you want to grow.

If you PC several jars and leave them un-noced at room temp one or some of them may have visible beasties growing in there in a couple weeks. 


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OfflineRotnpins
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Re: Cultivation General Discussion [Re: learningabroad]
    #27851363 - 07/05/22 11:38 PM (1 year, 6 months ago)

Quote:

learningabroad said:
Newbie with one semi-successful grow. I have a few questions that I hope could be answered here. I've been reading through the different beginner guides but have not found answers I'm looking for. In my first batch ever I had used syringe spores (LI?) into 4 different substrate jars purchased online.. all four of them produced healthy mycelium . I transferred each jar into its own lasagna tray and mixed with Organic mushroom compost and placed in monotub ( after reading some of the monotub teks I think I might have been way off. A friend of mine said to put a inch of water in the bottom of the tub let the lasagna trays float in the water and mist the sides so that they are constantly wet). 2 trays had some success and grew fruits, but developed trich (green mold) after first flush. As it was all going green there was one final burst and I got two ginormous fruits. The other 2 lasagna trays in the other monotub literally did nothing at all. I did the same misting and the same Fanning that I did every day to the first monotub and yet this one literally did nothing at all and never turned white or formed into a cake or produced fruit. Does this happen to people sometimes where even when the growth in a substrate jar looks healthy it doesn't go well when put into its I guess people would call a fruiting chamber? ( sorry still learning all the lingo).

The other issue I recently ran into was I did the same exact procedure when I tried to start a new batch and I inoculated four substrate jars again with a single syringe. One jar immediately exploded with growth and then turned green. A second jar took maybe 2 weeks longer and then it finally started producing mycelium. It is still going strong and is nearing its time to transfer to the monotub, this time I will be using bodhisattva's Tek. However the remaining two jars have not changed even the slightest indicating no signs of mold or mycelian growth in over a month. Did I do somethingwrong? As a somewhat hasty last ditch effort I took another syringe and decided to inoculate one of these last two remaining jars with the entire contents of the syringe. That might have been a huge waste of money and very stupid on my part, again I am a total newbie.

Going forward I think it's going to be important to rely on Spore prints and agar which leads me to my next question. I just want to clarify that I have the right idea here because this isn't totally clear reading through some of these posts. If I make a Spore print from a fresh cap placed on tin foil do I want to store that tin foil sheet in the fridge sealed to preserve it? Or do I immediately put it on agar and keep the agar in a fridge until I want to start the next batch? I'm not looking to inundate myself with hundreds of jars and agar discs floating around my apartment. As some of you have pictures to prove. I just need clarification on the process of obtaining Spore prints in relation to using agar and how to preserve prints for a long time so I can Pace myself and create new mycelium growth when I feel it's appropriate and needed.

Thank you for reading and I appreciate the communities efforts to help one another out. God bless.




You've definitely got the right idea starting agar work.. I would recommend reading the first link in my signature...

As far as spore prints.. using my SAB i usually put a cap on foil for 12 hours, then put the cap on a 2nd piece for an additional 12 hours... once you remove the cap, allow each sheet to dry for 24 hours before folding the print for storage.. I label my prints and then store them in ziplock bags and them I toss those ziplocks in a plastic "shoebox" tub.. I'm sure there are other ways of doing this, I'd recommend using the search function and looking for a tek on how to take and store spore prints...

When you go to use the print... I recommend using an inoculation loop... there are a few different methods.. I usually flame the loop, dip it in my agar plate to cool, scrape the print, and then streak a quarter of the plate in a zigzag.. then I repeat, moving over on the plate but doing a little overlap on the previous quarter of the plate.. I overlap enough to get 5 sections that are about 1/4 of the plate each... hopefully that makes sense

Another method I use is the same, but instead of streaking the plate after scraping the print I just stab the agar in different places to deposit spores...

I'd also recommend checking out the other links in my signature, especially the ones on agar transfers

Best of luck :cheers:


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Offlinespirit_shadow
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Re: Cultivation General Discussion [Re: wild_aussie]
    #27851648 - 07/06/22 08:25 AM (1 year, 6 months ago)

Quote:

wild_aussie said:
So I'm still a noob but I have a few grows and I have noticed something that used to really bother me.  When reading teks I noticed the approx times given weren't close to my actual times ei grain jars should be fully colonized in about 10-14 days my experience has been 28-32 days same thing with colonization times of pf jars, agar plates, even lc (mine is at 3 weeks and can just see trails of light cloudiness I hope is clean mycelium).  So should I just accept that is how things work in the conditions I'm in since it's so consistent or is there some key component Im missing?  I know this is an opinion question and I'd love to here then. Thanks



Have you been using the same spores or have you tried different varieties? What are the conditions of your immediate surroundings? What is the ambient temperature of the room your jars are in?



Edit: you can make your own inoculation loop out of an old guitar string and xacto knife handle if you play guitar :thumbup:


I am lazy and just have been using an  xacto knife to make syringes :lol:


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OfflineAuron
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Re: Cultivation General Discussion [Re: spirit_shadow]
    #27851759 - 07/06/22 10:05 AM (1 year, 6 months ago)

Quote:

spirit_shadow said:
Quote:

Auron said:
Can’t seem to decide on jars or a mono tub/bulk

How much more does one generally yield in a monotub vs. 12 jars?

Not sure I want to keep buying and dealing with perlite plus I’m gone for 9.5 hours a day and won’t be able to mist.



Click the shoebox link in my sig :thumbup:




Can I do that with a spawn bag? If so how big of a bag? I’m asking because I plan to transfer from a cleaned up agar plate and thought it would be best to make a syringe from that. But obviously I’m new


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OfflineDERRAYLD
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Re: Cultivation General Discussion [Re: Auron] * 1
    #27851768 - 07/06/22 10:10 AM (1 year, 6 months ago)

Slow down bud, that is waaaaay off.

You take the agar and cut a pizza wedge which you then insert into the grain bag.
All of this requires sterile procedures.


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OfflineAuron
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27852026 - 07/06/22 01:11 PM (1 year, 6 months ago)

Quote:

DERRAYLD said:
Slow down bud, that is waaaaay off.

You take the agar and cut a pizza wedge which you then insert into the grain bag.
All of this requires sterile procedures.




I like your jars better. I have plenty of 8oz jars that won’t get used because I’m deciding against cakes. Will using 2 of those be ok instead of the 16oz’ers you used?


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Offlinespirit_shadow
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Re: Cultivation General Discussion [Re: Auron]
    #27852044 - 07/06/22 01:22 PM (1 year, 6 months ago)

You can take sterile water in a syringe and take contam free myc in a SAB and suck said myc into syringe. I used to do that a lot back when I first messed with pasty plates :lol:


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Offlinespirit_shadow
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Re: Cultivation General Discussion [Re: spirit_shadow]
    #27852047 - 07/06/22 01:23 PM (1 year, 6 months ago)

I use pf cakes to shoebox as it's cheap and super easy and fast to make the jars :shrug:


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OfflineAuron
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Re: Cultivation General Discussion [Re: spirit_shadow]
    #27852074 - 07/06/22 01:41 PM (1 year, 6 months ago)

Quote:

spirit_shadow said:
I use pf cakes to shoebox as it's cheap and super easy and fast to make the jars :shrug:




He mentions 3/4 qt jars. Can’t seem to find any, what’s the ideal size?


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Offlinespirit_shadow
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Re: Cultivation General Discussion [Re: Auron]
    #27852082 - 07/06/22 01:43 PM (1 year, 6 months ago)

I use half pint wide mouth jars (I will use 3 or 4 per shoebox personally :shrug: )

Z strain in a pf jar


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OfflineSpank
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Re: Cultivation General Discussion [Re: spirit_shadow]
    #27852233 - 07/06/22 03:26 PM (1 year, 6 months ago)

Hey, I'm trying to get agar plates going for the first time. Every plate from one swab is showing some kind of aggressive contamination, probably bacteria. I also think the agar plates I used might not be optimal for spore germination, at 2% agar, 2% karo, 0.4% potato flake. The unopened plates and the plates from other swabs are looking fine 5 days after PC/inoculation, no type of growth, so germination might just be slow and I'll let them do their thing.

Should I just clean out the contaminated plates and try the second swab in the set, or should I wait to see if there's any germination? It's quickly becoming a gross-looking goopy mess. (I was also dumb and put the first swab back into the sleeve after inoculating)
Also, should I make softer, richer agar for germinating spores and save these other plates for when I have actual cultures?

Thanks, and hopefully this is the right place to ask this stuff!

Edit: one additional question, can I add an extra like 2% weight blender-powdered coffee in place of carbon/food coloring for the agar?

I'm gonna try blending the potato flakes before mixing because it's hard to distinguish what's on the surface vs. suspended/settled sediment.


--------------------


Edited by Spank (07/06/22 03:56 PM)


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OfflineAuron
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Re: Cultivation General Discussion [Re: spirit_shadow]
    #27852285 - 07/06/22 04:04 PM (1 year, 6 months ago)

Quote:

spirit_shadow said:
I use half pint wide mouth jars (I will use 3 or 4 per shoebox personally :shrug: )

Z strain in a pf jar




Does using more jars increase or decrease chances of contamination?


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OfflineSirPsycho
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Re: Cultivation General Discussion [Re: Auron]
    #27852286 - 07/06/22 04:07 PM (1 year, 6 months ago)

Quote:

Auron said:
Quote:

spirit_shadow said:
I use half pint wide mouth jars (I will use 3 or 4 per shoebox personally :shrug: )

Z strain in a pf jar




Does using more jars increase or decrease chances of contamination?



Does rolling a die multiple times change the odds of each number?


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: spirit_shadow]
    #27852311 - 07/06/22 04:30 PM (1 year, 6 months ago)

Quote:

spirit_shadow said:
You can take sterile water in a syringe and take contam free myc in a SAB and suck said myc into syringe. I used to do that a lot back when I first messed with pasty plates :lol:




Sucking myc? You’d be sucking in air as well. Dirty air.


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Offlinespirit_shadow
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Re: Cultivation General Discussion [Re: Auron]
    #27852508 - 07/06/22 06:55 PM (1 year, 6 months ago)

Quote:

Auron said:
Quote:

spirit_shadow said:
I use half pint wide mouth jars (I will use 3 or 4 per shoebox personally :shrug: )

Z strain in a pf jar




Does using more jars increase or decrease chances of contamination?



If you make the jars proper then the amount of jars wouldn't matter because your contam would be from the spores. It makes it easier to separate contam from spores via syringe then get clean spawn then get a clone from that or whatever :thumbup:


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Offlinespirit_shadow
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Re: Cultivation General Discussion [Re: mushboy]
    #27852511 - 07/06/22 06:57 PM (1 year, 6 months ago)

Quote:

mushboy said:
Quote:

spirit_shadow said:
You can take sterile water in a syringe and take contam free myc in a SAB and suck said myc into syringe. I used to do that a lot back when I first messed with pasty plates :lol:




Sucking myc? You’d be sucking in air as well. Dirty air.



Pastyplates didn't have dirty air via the one drop method in a very sterile SAB :p (once I got clean myc from a drop, obviously hit or miss getting clean myc per plate)


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OfflineSalty Dog
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mycelium transfer [Re: spirit_shadow]
    #27852875 - 07/06/22 10:10 PM (1 year, 6 months ago)

This may be a dumb question.  Can I transfer grain spawn to additional jars and expand my spawn?


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