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Offlinenektar61S
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Re: Cultivation General Discussion [Re: Healing Oakland]
    #27821319 - 06/15/22 08:06 PM (1 year, 7 months ago)

Quote:

Healing Oakland said:
Quote:

A.k.a said:
In pretty sure senescence is mostly linked to expansion, like g2g over and over. Putting them in cold storage is like hitting pause on the culture.




Also continuously taking clones of clones of clones over and over and over




Someone here said that isn't as much of an issue for cubes as with other shrooms. Anyone remember who?

Also, how come that huge natural fungus colony in Oregon doesn't wilt? It's all clones of clones, isn't it? It's supposed to be the largest organism in the world.


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Invisiblemilkboy
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Re: Cultivation General Discussion [Re: fahtster]
    #27821324 - 06/15/22 08:07 PM (1 year, 7 months ago)

Ok so is it bad practice to give them away? Or maybe include a disclaimer? Didnt realize my sw syringe was so precious...


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: milkboy] * 1
    #27821348 - 06/15/22 08:18 PM (1 year, 7 months ago)

I've given them away many times, I just give a disclaimer.


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Invisiblemilkboy
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Re: Cultivation General Discussion [Re: Crackatoa] * 1
    #27821359 - 06/15/22 08:22 PM (1 year, 7 months ago)

I feel like less of an asshole now. So the question becomes what can we do about it?

Where do stable APE spores come from? Is there some sort of exclusive culture?


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OfflineRotnpins
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Re: Cultivation General Discussion [Re: milkboy]
    #27821378 - 06/15/22 08:30 PM (1 year, 7 months ago)

Quote:

milkboy said:
Alright boys, girls and teapots, Ive been talking with a friend about APE spores and Im starting to think I fucked up.

In the past I have swabed APE fruits and given them away thinking nothing of it. Now Im starting to believe these are destined for failure, as in they wont produce APE fruits. In fact one person has reached out with a shoebox that was growing regular PEs. Is this true? I always assumed APE syringes were made with swabs.

Have I sined?





Wait? So how do they get the syringes not to revert? I'm glad you mentioned this because I was about to take some APE swabs in a few days once my pins mature.. now I think I need to read up a little more on reverts and APE swabs in general


Edit.. milkboy beat me to it, and he worded it better.


Edited by Rotnpins (06/15/22 08:31 PM)


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OfflineYoshiTrainer
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Re: Cultivation General Discussion [Re: Rotnpins] * 1
    #27821436 - 06/15/22 08:59 PM (1 year, 7 months ago)

My grain jar usually has a mix of oats, barley, millet and annual RGS in it. :smile:


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OfflineHealing Oakland
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Re: Cultivation General Discussion [Re: nektar61]
    #27821454 - 06/15/22 09:11 PM (1 year, 7 months ago)

Yeah Nectar I don't know fr fr, I'd be interested in seeing something conclusive, I've never pushed it to extremes to actually know for myself. It may just be information I've blindly believed because it sounded about right along side the continuous grain expansion bit.

As for the Oregon colony, they drop spores to continue the life cycle/expansion like that. Cloning is a human/cultivator interaction with mushrooms. :cheers:




edit: typo


Edited by Healing Oakland (06/16/22 11:29 AM)


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Offlinenektar61S
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Re: Cultivation General Discussion [Re: milkboy] * 1
    #27822309 - 06/16/22 10:49 AM (1 year, 7 months ago)

Quote:

milkboy said:
Ok so is it bad practice to give them away? Or maybe include a disclaimer?




You aren't just using them for microscopic use only??

An accurate disclaimer with all spore giveaways would be:

"These are free mushroom genetics, tweaked over and over in people's closets for hundreds of generations. They are as far from shrooms growing in the wild as a teacup poodle is from a timber wolf, even though a poodle and a wolf are basically the same species."

"But unlike the poodle, these spores are almost guaranteed to quickly throw up mutations and reverts. Please enjoy these free crazy spores, no guarantee implied."

Beyond that, any Penis Envy or anything derived from Penis Envy (like the APE Milkboy was mentioning) is more likely to have some inconsistent results.


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Edited by nektar61 (06/16/22 03:14 PM)


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InvisibleShewey
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Re: Cultivation General Discussion [Re: nektar61] * 1
    #27822534 - 06/16/22 01:10 PM (1 year, 7 months ago)

stupid question but what is "harboring endospores" so you can get away with not presure cooking rice and popcorn at 15 psi because they dont harbor endospores but what does that mean. arent there mold spores all over the air, so loading them up in jars in open air would mean spores would get into jars right?


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OfflineNichrome
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Re: Cultivation General Discussion [Re: Shewey] * 4
    #27822642 - 06/16/22 02:05 PM (1 year, 7 months ago)

A lot of people use words for microscopic things they have never ever seen. It is easy to blame symptoms on things you can't see, a lot easier than actually figuring out why you are seeing what you are.

Endospores in grain, enmeshed contamination, blah blah. Nobody is talking about arsenic levels in grain hulls, or phytic acid content, or anything like that. The go to is mold, bacteria, or magical endospores. Mold, bacteria, and endospores are indeed real but things are a lot more complex than any of us know and it is a deep dive to try to understand. A deep deep dive. I have seen a lot of things and still don't know diddly and make mistakes all the time.

A brain is a great instrument. Impacts add up over time.


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InvisibleSwabMarleyS
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Re: Cultivation General Discussion [Re: Nichrome]
    #27822654 - 06/16/22 02:13 PM (1 year, 7 months ago)

@Nichrome You’re dropping the fuckin knowledge today man! Sheeeeeiiiiit!
:snoopyes:


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OfflineRotnpins
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Re: Cultivation General Discussion [Re: SwabMarley]
    #27822711 - 06/16/22 02:32 PM (1 year, 7 months ago)

Quote:

Myc Hunt said:
@Nichrome You’re dropping the fuckin knowledge today man! Sheeeeeiiiiit!
:snoopyes:




:whathesaid:


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Offlinenektar61S
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Re: Cultivation General Discussion [Re: Nichrome]
    #27822809 - 06/16/22 03:19 PM (1 year, 7 months ago)

Quote:

Nichrome said:
A lot of people use words for microscopic things they have never ever seen. ..




In addition to all that Nicrome added here, I think endospores get mentioned more than these other vectors because of an old, wrong tek.

There was a debunked tek people did on here a decade or more ago where they let grain sit to "grow the endospores" so they could "kill them once they're growing", and some teks did this more than once.

I think some of this is still propagated because some people wrote books that were cutting edge 10 or 15 years ago ago, are still in circulation, at least as PDFs, plus some videos on DVD and YouTube, but are horribly out of date with best practices now, even by the people who created them.

None of that is needed. 2 hours at 15 psi in a PC (plus a little more time if you're far above sea level) kills it all.

Correct post from 7 years ago by Bod:

Quote:

A sufficient and correctly done Autoclave/PC cycle WILL kill endospores

endospores DO NOT need to be "softened" "soaked" "hatched" "germianted" etc... to be killed






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OfflineAdas
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Re: Cultivation General Discussion [Re: nektar61] * 2
    #27822830 - 06/16/22 03:33 PM (1 year, 7 months ago)

I think that was the idea behind fractional sterilization. Certainly all you need is to PC them, but if you don't have a pressure cooker that's what this method is for. I've used it before I've had a PC and it somewhat worked. Still got bacteria most times, but the myc was usually able to make it.


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Offlinenektar61S
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Re: Cultivation General Discussion [Re: Healing Oakland]
    #27822841 - 06/16/22 03:38 PM (1 year, 7 months ago)

Quote:

Healing Oakland said:
Yeah Nectar....As for the Oregon colony, they drop spores to continue the life cycle/expansion like that. Cloning is a human/cultivator interaction with mushrooms. :cheers:





Quote:

It's not just the same type of fungus. DNA testing has convinced the scientists this is the largest single living organism in the world.



https://www.opb.org/television/programs/oregon-field-guide/article/oregon-humongous-fungus/

Same is true of the largest aspen forest too.

Cloning wasn't the right term for me to use. But wouldn't  senescence be at play as an organism spreads out as far as the mushroom or aspen tree organism? Both seem to be doing great.


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OfflineNichrome
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Re: Cultivation General Discussion [Re: Adas] * 4
    #27822855 - 06/16/22 03:45 PM (1 year, 7 months ago)

:highfive1:

In some situations it working sometimes, is all you can hope for anyway. I talk to folks regularly who don't have access to things like pressure cookers and stuff like that but they need to make it work anyway, so they can eat or make money to eat or just find solace in life's ocean of trouble.

I fully support whatever you can do to make something work even if it is not ideal.

Shit, one time I rigged a coffee straw to a turkey baster with some masking tape and elbow grease and noc'd a stack of pf cakes. Worked like a charm. I can't say that is in any way a best practice but it was the best practice at the time because it worked.

Always more than one way to crack a coconut.

(disclaimer: I've been munching small amounts of various tampanensis mushrooms slowly over the course of the day...:heart:)


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Offlinedreamwever
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Re: Cultivation General Discussion [Re: Nichrome] * 1
    #27822891 - 06/16/22 04:07 PM (1 year, 7 months ago)

:mushroom2:

Got a pressure cooker yesterday on sale, only 12psi, so maybe 45mins for the 500ml media bottle of agar?

Then i think it is let to cool a little ( not sure in a PC, how long that is for? ) so i can pour it in the SAB to the petri dishes, wrap with cling wrap and letthem cool in the SAB, then next day inoculate with wild cubensis spares, wrap with parafilm store in pantry either in torte or open glad bags

Then clean up agar and get ready for the next steps :laugh:


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Offlinecozmyc
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Re: Cultivation General Discussion [Re: Nichrome]
    #27822938 - 06/16/22 04:37 PM (1 year, 7 months ago)

Quote:

Nichrome said:


Always more than one way to crack a coconut.

(disclaimer: I've been munching small amounts of various tampanensis mushrooms slowly over the course of the day...:heart:)




I love knowledge. I'm with you my friend, cubes. :mushroom2:


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InvisibleShewey
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Re: Cultivation General Discussion [Re: cozmyc]
    #27823001 - 06/16/22 05:23 PM (1 year, 7 months ago)

Im doing all my sterilization in an instant pot and ive tried wbs but got contam so i always go overkill with agar and grain but im asking cus ive gotten contaminated plates after a 90 minute cycle. They were no pours too but im now starting to think something jus happened to get in. Thanks for the knowledge nichrome


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Offlinenektar61S
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Re: Cultivation General Discussion [Re: Inocuole]
    #27823030 - 06/16/22 05:51 PM (1 year, 7 months ago)

If senescence is a big problem with cube clones, wouldn't all Enigma suck?

From what I can tell, every Enigma culture is a clone of a clone of a clone of a clone etc etc, unless the person who first made it is sending out thousands of cultures.


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