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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
Re: Cultivation General Discussion [Re: Huskies]
    #27799340 - 05/30/22 05:30 PM (1 year, 7 months ago)

Quote:

Huskies said:
^ Dope, is this the appropriate tek?

https://www.shroomery.org/forums/showflat.php/Number/26254384/fpart/1/vc/1

On another note, is an Instant Pot enough for Agar work? It is what I have been doing, and I have great success. ANy contam is virtually due to some hiding bacteria that I have gotten rid of.

that being said, if I am somehow not getting them sterilized after 45 mins at probaly 12psi that might be an issue




There's a much better thread that goes into detail but I can't find it rn and I'm tired as fuck.

If you search agar instant pot then somebody will have shared times but 45 min sounds about right, idk tho.

Only way to tell is by leaving a control plate untouched for a while, if that doesn't contam then its your process.


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Being pissed on does not make you a real man.


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InvisibleNimpo
Big Black


Registered: 05/10/12
Posts: 2,375
Re: Cultivation General Discussion [Re: Wildcat1883]
    #27799349 - 05/30/22 05:41 PM (1 year, 7 months ago)

Quote:

Wildcat1883 said:
Is shaking the jar necessary or only to speed up the process?




It can help speed up colonization.

If can also help spot contaminated spawn. If it doesn’t recover, it’s a tosser


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Invisiblejoze
Male


Registered: 11/10/20
Posts: 928
Loc: PNW
Re: Cultivation General Discussion [Re: Huskies]
    #27799412 - 05/30/22 06:40 PM (1 year, 7 months ago)

Quote:

Huskies said:
^ Dope, is this the appropriate tek?

https://www.shroomery.org/forums/showflat.php/Number/26254384/fpart/1/vc/1

On another note, is an Instant Pot enough for Agar work? It is what I have been doing, and I have great success. ANy contam is virtually due to some hiding bacteria that I have gotten rid of.

that being said, if I am somehow not getting them sterilized after 45 mins at probaly 12psi that might be an issue




I get great results using an Instant Pot for agar. I only run my agar for 30 mins at 12.5 psi and I've never had issues with contaminants on my plates.


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Offlinewild_aussie
Punk rocker til I die
I'm a teapot User Gallery


Registered: 01/05/22
Posts: 1,000
Loc: Skatepark grinding hard
Last seen: 5 days, 23 hours
Re: Cultivation General Discussion [Re: Huskies]
    #27799675 - 05/30/22 11:56 PM (1 year, 7 months ago)

Quote:

Huskies said:
^ Dope, is this the appropriate tek?

https://www.shroomery.org/forums/showflat.php/Number/26254384/fpart/1/vc/1

On another note, is an Instant Pot enough for Agar work? It is what I have been doing, and I have great success. ANy contam is virtually due to some hiding bacteria that I have gotten rid of.

that being said, if I am somehow not getting them sterilized after 45 mins at probaly 12psi that might be an issue



I've used instapot for agar just did 60 min pressure cook high pressure. And have gotten no contam from sterilization just some of my sterile work so yes instapot works fine I run for 60min but less might work


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Offlinehazyhorse
scoobin
I'm a teapot Unread Journal User Gallery


Registered: 03/19/19
Posts: 3,820
Last seen: 1 day, 15 hours
Re: Cultivation General Discussion [Re: wild_aussie] * 1
    #27799724 - 05/31/22 01:44 AM (1 year, 7 months ago)

here's pastywhyte's development of rustywhyte if that might help at all. i remember a thread involving workman about the creation of APE that was really good too but don't have the link handy


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OfflineBleuMojeaux
Futzer of Things
Male


Registered: 03/20/22
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Re: Cultivation General Discussion [Re: Inocuole]
    #27802854 - 06/02/22 03:48 PM (1 year, 7 months ago)

Should I toss these?  I opened 1 of about 6 that look like this and it smells fine...just looks off.



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InvisiblemushboyMDiscord
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Registered: 04/24/05
Posts: 32,281
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Re: Cultivation General Discussion [Re: BleuMojeaux]
    #27802881 - 06/02/22 04:08 PM (1 year, 7 months ago)

I’d top fruit those.


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OfflineBleuMojeaux
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Registered: 03/20/22
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Re: Cultivation General Discussion [Re: mushboy]
    #27802889 - 06/02/22 04:14 PM (1 year, 7 months ago)

I'll look into that... Thanks


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InvisibleMr Piggy
Big Dick Retard
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Registered: 09/29/11
Posts: 8,401
Re: Cultivation General Discussion [Re: Guerrilla] * 1
    #27802891 - 06/02/22 04:14 PM (1 year, 7 months ago)

Quote:

Guerrilla said:
Just found my old print collection, anybody know what TPO and TKS





I had great luck with Thai Koi Samui.  Had some killer cultures I should never have let go.


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OfflineBleuMojeaux
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Re: Cultivation General Discussion [Re: Inocuole]
    #27802909 - 06/02/22 04:24 PM (1 year, 7 months ago)

If I had the shitty luck of receiving a print or a syringe that produces fruits with weak potency, can anything I do change this or should I just start again with a new print.


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OfflineAnkhseramun
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Other


Registered: 10/11/21
Posts: 589
Loc: Cantina, Mos Eisley, Tatooine
Last seen: 1 year, 7 months
Re: Cultivation General Discussion [Re: BleuMojeaux] * 3
    #27802924 - 06/02/22 04:31 PM (1 year, 7 months ago)

Don't drop the print just yet. If it was indeed the genetics then another plate made from the same spores will have completely different genetics than the first one.
But if the second one is a bust too, then I'd stop working on them and look for some new spores.


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InvisibleMysticMycologist
Dirt Sherpa
Male


Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
Re: Cultivation General Discussion [Re: BleuMojeaux]
    #27802957 - 06/02/22 04:50 PM (1 year, 7 months ago)

Just go back to spore from the same print. Every two spores in a print will make strain, and the fruits from the same tub, even the same flush from MS can have a lot of expressed variability. Once you get some fruits you like, make a clone culture and run that.


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Offlinehazyhorse
scoobin
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Re: Cultivation General Discussion [Re: BleuMojeaux] * 2
    #27802959 - 06/02/22 04:52 PM (1 year, 7 months ago)

also just curious, how were they dried & stored? improper drying & storing can def kill potency


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineAnkhseramun
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Posts: 589
Loc: Cantina, Mos Eisley, Tatooine
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Re: Cultivation General Discussion [Re: hazyhorse]
    #27802968 - 06/02/22 04:58 PM (1 year, 7 months ago)

:whathesaid:
Definitely this. In the overwhelming majority of cases the lacking potency was due to improper drying and storage techniques.
I've only rarely heard about mushrooms being weak from the get go due to potency.


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OfflineBleuMojeaux
Futzer of Things
Male


Registered: 03/20/22
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Re: Cultivation General Discussion [Re: hazyhorse]
    #27802971 - 06/02/22 04:58 PM (1 year, 7 months ago)

Immediately dried in dehydrator to crispy cracker dry and ate the next day.


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OfflineAnkhseramun
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Registered: 10/11/21
Posts: 589
Loc: Cantina, Mos Eisley, Tatooine
Last seen: 1 year, 7 months
Re: Cultivation General Discussion [Re: BleuMojeaux]
    #27802979 - 06/02/22 05:03 PM (1 year, 7 months ago)

Hmmmm you might be one of the few occasions I've read/heard about inherently weak mushrooms, congratulations
:borat:

Then all points towards weird genetics. Knock up a new plate with the same spores and it should be better. Good luck!

Out of curiosity: Did they bruise like usual when handling them?


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OfflineBleuMojeaux
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Registered: 03/20/22
Posts: 71
Last seen: 1 year, 4 months
Re: Cultivation General Discussion [Re: Ankhseramun]
    #27802989 - 06/02/22 05:05 PM (1 year, 7 months ago)

This is what I'm thinking also.  Yes blue bruising and all.  PESH from a sponsored vendor.  The syringe was jacked up to begin with.  Looked like it had been sitting in storage for years or some shit.


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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Cultivation General Discussion [Re: BleuMojeaux] * 1
    #27802991 - 06/02/22 05:06 PM (1 year, 7 months ago)

I grew some Tidalwaves last year which did absolutely nothing when consumed. Many friends tried them with zero effect, even doses of 7gs.


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OfflineAnkhseramun
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Registered: 10/11/21
Posts: 589
Loc: Cantina, Mos Eisley, Tatooine
Last seen: 1 year, 7 months
Re: Cultivation General Discussion [Re: BleuMojeaux]
    #27802995 - 06/02/22 05:09 PM (1 year, 7 months ago)

If they turn out to be bunk you are just around the corner to unlock the marketplace either way, so you'll be decked out on spores for life soon.
Also you can hit me up, I have three freshly made AA+ prints from a culture that produced crazy amounts of pretty strong microscope slides.

Quote:

Guerrilla said:
I grew some Tidalwaves last year which did absolutely nothing when consumed. Many friends tried them with zero effect, even doses of 7gs.



And you're the next one in the book. It seems I might've just not read enough on the topic :lol:


Edited by Ankhseramun (06/02/22 05:10 PM)


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OfflineBleuMojeaux
Futzer of Things
Male


Registered: 03/20/22
Posts: 71
Last seen: 1 year, 4 months
Re: Cultivation General Discussion [Re: Guerrilla]
    #27802996 - 06/02/22 05:09 PM (1 year, 7 months ago)

I've always read about shitty genetics but my luck, I get some on my 2nd try at a grow ffs.


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