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mushboy
modboy



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I’ve poured LC in a sab all the time.
You don’t gotta worry about a vacuum if you wrap the lid in foil and keep it loose pc 20min. Tighten down after it cools. Like agar. same thing only minus the AGAR. Pour like agar. Easy peasy derp
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Doctor Mario
🍄🍄🍄🍄🍄



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Re: Cultivation General Discussion [Re: mushboy]
#27778155 - 05/15/22 11:41 AM (1 year, 8 months ago) |
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 I only use SSLC in unmodified pint jars. PC the way that Mushboy is explaining and tighten the lids after the jars cool. If you tighten the lids before sterilizing, please record yourself opening the jar and send me the vid so that I can see that I'm not the only one that's had that struggle lol. I will occasionally not loosen one of the lids enough and have had to use my belt to get it off. An oil filter wrench would be good in that situation.
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SwabMarley
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Re: Cultivation General Discussion [Re: mushboy] 1
#27778165 - 05/15/22 11:51 AM (1 year, 8 months ago) |
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I’m glad Mushboy has chimed in because his thread on LC’s is where I pretty much learnt everything about LC’s.
Respect!
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ichugwindex
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Re: Cultivation General Discussion [Re: Doctor Mario]
#27778166 - 05/15/22 11:52 AM (1 year, 8 months ago) |
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I feel like free pouring can certainly be done but it also seems like a sure fire way for a noob to mess things up in a SAB.
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
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Tight Lunchbox
Drunk cat


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Re: Cultivation General Discussion [Re: ichugwindex]
#27778176 - 05/15/22 11:56 AM (1 year, 8 months ago) |
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I suppose it depends on their understanding of sterile technique inside a sab. If someone can successfully do transfers or noc up jars with wedges then they can inoculate a bottle and pour without any trouble.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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mushboy
modboy



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Re: Cultivation General Discussion [Re: ichugwindex] 1
#27778211 - 05/15/22 12:27 PM (1 year, 8 months ago) |
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Quote:
ichugwindex said: I feel like free pouring can certainly be done but it also seems like a sure fire way for a noob to mess things up in a SAB.
Nah no more than pouring agar. Which is extremely easy and noob friendly.
Children can do this shit in science class don’t over complicate a basic task
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Josex
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Re: Cultivation General Discussion [Re: ichugwindex]
#27778213 - 05/15/22 12:27 PM (1 year, 8 months ago) |
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Quote:
ichugwindex said: I feel like free pouring can certainly be done but it also seems like a sure fire way for a noob to mess things up in a SAB.
That's the only thing I used to do when I was a noob, pouring LC in a SAB. Shaking hands and all, awesome success... I use syringes these days because I like to be exact and because I actually sell LC syringes,but pouring in a SAB works like a charm.
Before you fall prey to your own fears and "logic" (this can't work in a SAB, not with LC at least...) you need to give it a serious try first. The fact that anyone can have damn near 100% success with agar transfers in a SAB should be enough to make you wanna try other procedures that should not be any different in practice.
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ichugwindex
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Re: Cultivation General Discussion [Re: mushboy]
#27778225 - 05/15/22 12:33 PM (1 year, 8 months ago) |
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When i pour tea out of a pot on the stovetop into a glass i almost always have a little liquid pour down the side of the pot missing the glass. This is where my fear lies with just pouring in a SAB. Obviously you guys are doing this just fine but i could definitely see myself fucking that up
I think Josex may be right in that i am being overly fearful
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
Edited by ichugwindex (05/15/22 12:41 PM)
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sandman420
Saint PP



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Re: Cultivation General Discussion [Re: mushboy]
#27778282 - 05/15/22 01:04 PM (1 year, 8 months ago) |
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Quote:
mushboy said:
Quote:
ichugwindex said: I feel like free pouring can certainly be done but it also seems like a sure fire way for a noob to mess things up in a SAB.
Nah no more than pouring agar. Which is extremely easy and noob friendly.
Children can do this shit in science class don’t over complicate a basic task
That's not really a fair comparison. Some satellite contamination is not really a problem when you are growing bacteria colonies in a science class.
Yea anyone can pour some plates open air and grow bacteria in a few days and go all ewwh-ahh before it all goes green.
Personally I found pouring agar in a SAB to be very prone to failure and would highly prefer doing no pours if that's all I had. Like 100 times prefer.
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A.k.a
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Re: Cultivation General Discussion [Re: sandman420]
#27778285 - 05/15/22 01:05 PM (1 year, 8 months ago) |
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Failure in a SAB is almost always gonna be a technique issue.
If you can do one thing in there you should be able to do most others.
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LAGM2020     
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sandman420
Saint PP



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Re: Cultivation General Discussion [Re: A.k.a]
#27778306 - 05/15/22 01:15 PM (1 year, 8 months ago) |
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Just have to politely disagree with that. I could transfer all day and do no pours perfectly but never could get pouring down.
I'd lose dunno 20-50% or so pre-use in storage so it all went bad in the pour.
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SwabMarley
Twisted Metal Head



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Re: Cultivation General Discussion [Re: A.k.a]
#27778312 - 05/15/22 01:16 PM (1 year, 8 months ago) |
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You don’t even really need great technique to free pour LC into a jar with success. I can pour that shit in way less than a second every time. As in, that jar is open for tenths of a second, the lid of the jar always remains in your hand so is sheltered from falling contam. The only risky part is uncapping the media bottle. Like someone just mentioned though, it’s no more than transferring to a petri and petri’s are usually 90mm where as the opening of a media bottle is more along the lines of 30mm.
A media bottle also has a pouring ring so no mess. Granted you have to recap the bottle but if you at least have a moderate sized Sab you can inoculate 3 jars with 1 cap removal/pour no issue.
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Culliganman360
Stranger



Registered: 06/05/19
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Quote:
Tight Lunchbox said: LC has nutes. You don't know what you're talking about
Lacking of something is to be without or short supply
Know the words your using before u speak
As for the lids. Aren’t you essentially doing the same amount of work . Without modding a lid you have to take it out and tighten it when pc cools. If u just put a filter on the lid you can take it out days later if needed . Different strokes for different folks man yall on that one way mindset open up
Edited by Culliganman360 (05/15/22 01:38 PM)
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A.k.a
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Re: Cultivation General Discussion [Re: sandman420] 1
#27778374 - 05/15/22 01:47 PM (1 year, 8 months ago) |
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Quote:
sandman420 said: Just have to politely disagree with that. I could transfer all day and do no pours perfectly but never could get pouring down.
I'd lose dunno 20-50% or so pre-use in storage so it all went bad in the pour.
It’s funny how stuff works out like that. When I was doing SAB work pouring was the easiest for me. I rarely had contams from day one, but doing transfers I had a horrible contam rate for a long time.
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LAGM2020     
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Tight Lunchbox
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Quote:
Culliganman360 said:
Quote:
Tight Lunchbox said: LC has nutes. You don't know what you're talking about
Lacking of something is to be without or short supply
Know the words your using before u speak
As for the lids. Aren’t you essentially doing the same amount of work . Without modding a lid you have to take it out and tighten it when pc cools. If u just put a filter on the lid you can take it out days later if needed . Different strokes for different folks man yall on that one way mindset open up

Not having to mod a lid seems like less work to me.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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mushboy
modboy



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Re: Cultivation General Discussion [Re: ichugwindex]
#27778493 - 05/15/22 02:42 PM (1 year, 8 months ago) |
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Quote:
ichugwindex said: ….almost always have a little liquid pour down the side of the pot missing the glass. This is where my fear lies with just pouring in a SAB.
I used agar bottles for lc because they have those drip rings. I shared your concerns but after practice I started using glass salad dressing jars
Try not to dribble….
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ChildOfTheMoon
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Re: Cultivation General Discussion [Re: A.k.a]
#27778494 - 05/15/22 02:42 PM (1 year, 8 months ago) |
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Thanks for all the input!
I think when I finally get around to LC I’ll just pour it the first few times and modify a lid with a filter if I feel the need for filling syringes. Relatively new to the hobby (Nov/Dec ‘21), but my latest agar pouring round had had a 0% contam rate and I’ve been doing transfers etc just fine, so I gather that pouring LC will go fine too.
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shroomuser
Stranger


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Re: Cultivation General Discussion [Re: ichugwindex]
#27778501 - 05/15/22 02:45 PM (1 year, 8 months ago) |
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Quote:
ichugwindex said: When i pour tea out of a pot on the stovetop into a glass i almost always have a little liquid pour down the side of the pot missing the glass. This is where my fear lies with just pouring in a SAB. Obviously you guys are doing this just fine but i could definitely see myself fucking that up
I think Josex may be right in that i am being overly fearful
If you can spend some bucks on a media bottle, they often have a no-drip lip. It's nice.
Is it normal for APEs to get gold caps after dehydration? Am I over-dehydrating/roasting these things? I've been doing 160F for 30 hours. 160F for 24 hours has been working well for me with other cubes, and I read that these should go longer due to their thickness. Too much? These look ok? I wasn't expected so much to turn gold, but don't have much experience either.
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mushboy
modboy



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milkboy
Child



Registered: 05/01/21
Posts: 2,299
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Re: Cultivation General Discussion [Re: shroomuser]
#27778563 - 05/15/22 03:18 PM (1 year, 8 months ago) |
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Those APEs look great! You didnt do anything wrong, enjoy eating them 
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