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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 4 hours, 32 minutes
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Re: Cultivation General Discussion [Re: mushboy]
#27730881 - 04/11/22 10:42 PM (1 year, 9 months ago) |
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I've had 2 oat bags I made about a year and a half ago that still look perfectly fine. I kept them to see how they would do.
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Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
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Re: Cultivation General Discussion [Re: Dendrocopos]
#27730888 - 04/11/22 10:57 PM (1 year, 9 months ago) |
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I've been through hundreds of pasty plates, all prepared the same way with one layer of micropore, and the only contams I've had otherwise are from ms, transfers (once), and occasional bad plate handling technique. It does seem logical to conclude that they somehow got contamed before going into the bag.
I'm going to set up an experiment using the following:
- 5 no-pour half pint mason jars of agar with no GE hole and the lid-seal facing down.
- 5 pasty plates.
- 5 holy-grail-of-no-pour plates.
It will conclude with one of three results:
- No plates contam.
- Only the pasty plates and/or holy-grail plates contam.
- Plates of all 3 types contam.
I'll report back with the results in a year or two!
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Elf_on_a_Log]
#27730890 - 04/11/22 11:03 PM (1 year, 9 months ago) |
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Quote:
Elf_on_a_Log said: It does seem logical to conclude that they somehow got contamed before going into the bag.
why not? thats how shit contaminates. pp containers deform and shit.. mixed with a single layer of mp tape? id bet money every time on something going to shit while its on the way to your fort knox pastyplate storage system.
youve apparently been hot on this case for over 4yrs ill leave ya too it
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Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
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Re: Cultivation General Discussion [Re: mushboy] 2
#27730893 - 04/11/22 11:04 PM (1 year, 9 months ago) |
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Quote:
mushboy said:
Quote:
Elf_on_a_Log said: It does seem logical to conclude that they somehow got contamed before going into the bag.
why not? thats how shit contaminates. pp containers deform and shit.. mixed with a single layer of mp tape? id bet money every time on something going to shit while its on the way to your fort knox pastyplate storage system.
youve apparently been hot on this case for over 4yrs ill leave ya too it
I think you misread my post. I said it DOES seem logical.
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Elf_on_a_Log] 1
#27730895 - 04/11/22 11:05 PM (1 year, 9 months ago) |
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thats embarrassing
whelp heres to your year
Edited by mushboy (04/11/22 11:10 PM)
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Dj_karma_magnet


Registered: 04/03/19
Posts: 203
Last seen: 4 months, 6 days
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Re: Cultivation General Discussion [Re: Ankhseramun]
#27730899 - 04/11/22 11:13 PM (1 year, 9 months ago) |
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Quote:
Ankhseramun said: No need to really read anything, just take a shovel, find a good shady spot with not too much direct sunlight and bury the cakes and cover with something like 3-4 inches of earth/substrate (like a topping layer).
Okay. If I do this in may, when can I expect to see shrooms? Will they come back next year too?
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Ankhseramun
Wanderer



Registered: 10/11/21
Posts: 589
Loc: Cantina, Mos Eisley, Tatooine
Last seen: 1 year, 7 months
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Quote:
Dj_karma_magnet said:
Quote:
Ankhseramun said: No need to really read anything, just take a shovel, find a good shady spot with not too much direct sunlight and bury the cakes and cover with something like 3-4 inches of earth/substrate (like a topping layer).
Okay. If I do this in may, when can I expect to see shrooms? Will they come back next year too?
If the weather is good all the way through then 1-2 months later you should have mushrooms growing in there and yeas it is reoccuring so you'll have a spot to come back to yearly. Just make sure it's somewhere secluded so no one finds them and takes them before you come around
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 8 hours, 50 minutes
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Re: Cultivation General Discussion [Re: Dendrocopos] 1
#27731005 - 04/12/22 03:35 AM (1 year, 9 months ago) |
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Quote:
Dendrocopos said: Maybe the mold started practicing quantum tunneling. Are you sure there's not part of the story you're leaving out?
Great, so I'm not the only one who's ever seriously considered this before 
I think the mostl ikely scenario is that mold spores were present on the outside, but since you also put everything into a baggie the humidity got high enough that the spores germinated and the super-weak mycelia were creeping along the surfaces until they hit the dishes. I've seen molds grow on clean glass in high humidity before so this is not far fetched at all.
EDIT: Ah, micropore tape. That's the more likely culprit actually.
Edited by Adas (04/12/22 03:37 AM)
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
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Re: Cultivation General Discussion [Re: Inocuole]
#27731006 - 04/12/22 03:40 AM (1 year, 9 months ago) |
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I am most confused by the paper tissues. We're they under the micropore ? That quiet literally explains everything no? Molds were able to germinate because of it.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Elf_on_a_Log] 2
#27731045 - 04/12/22 05:36 AM (1 year, 9 months ago) |
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Quote:
Elf_on_a_Log said: Assuming you're correct, my original point has been reinforced: On a long enough time scale sterile grain jars will contam. If not by endospores, by mold spores that somehow find their way in.
We were talking about cheap glad rounds. Endospores and mold contamination on properly sterilized grains either in bags or in jars is a different story altogether.
Your lids and filter patches will long outlast the moisture content of your grains if sterilized correctly, as moosh/crack pointed out. If your grains were sterilized correctly they'll just dry up.
Edospores are destroyed with proper sterilization, mold dies long before endospores start to sweat. Mold can only enter through warped lids or faulty filters; which is why your glad rounds failed...glad rounds are cheap and flimsy. Given enough time your glad rounds will likey fail.
Edit:
I've had petri dishes wrapped in cling wrap last for over a year without contaminating. Same thing goes for pp5 slants; any contaminations will be user error.
Stop using cheap materials if your aim is longevity. Pasty plates are designed to get people up and going with agar without the initial investment in petri dishes, I could be wrong but I doubt pasty had long term storage in mind when he wrote that tek.
Edited by Stipe-n Cap (04/12/22 05:58 AM)
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
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Re: Cultivation General Discussion [Re: Inocuole]
#27731157 - 04/12/22 08:42 AM (1 year, 9 months ago) |
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Man, i dont know how much pasty rounds cost overseas but that shit will financially ruin you over here. 2 mini rounds cost around the same as a case of 25 petris.
Edited by Dendrocopos (04/12/22 08:57 AM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Dendrocopos]
#27731201 - 04/12/22 09:18 AM (1 year, 9 months ago) |
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20$ for 8 in Canada, but I was mostly referring to quality of design. Pp5 snap containers with mp tape filters aren't as reliable long term as wrapped plates, slants, or lids modified with synthetic filter disks....obviously.
A box of 500 petris cost me around 200$, I'd avoid purchasing that many glad rounds
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MTZ
In between



Registered: 12/17/21
Posts: 702
Loc: Northern rockies
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27731245 - 04/12/22 10:05 AM (1 year, 9 months ago) |
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Holy shit! Ahhh... I pay $60ish for 500. Maybe I should start smuggling cases of dishes. I'm on the boarder π Hmmm... New business opportunity π€
-------------------- Rehears your death every morning and night. Only when you constantly live as though already a corpse will you find freedom in the martial way, and fulfill your duties without fault throughout your life. ~ The Book Of Samurai ........ I live in the void. I can go to the in between anytime I desire. I visit the spirt world.
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
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Re: Cultivation General Discussion [Re: Inocuole]
#27731281 - 04/12/22 10:46 AM (1 year, 9 months ago) |
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Man Americe Is truly the place to be when you have obscure hobbies, wich are the only hobbies i have
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 8 hours, 50 minutes
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Re: Cultivation General Discussion [Re: Dendrocopos]
#27731342 - 04/12/22 12:16 PM (1 year, 9 months ago) |
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I've never modified my plastic agar container lids, ever. Do you guys think the extra gas exchange is really beneficial?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Adas]
#27731344 - 04/12/22 12:20 PM (1 year, 9 months ago) |
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The bottle that you pour agar from, like a media bottle?
No, I just loosen to pc.
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
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Re: Cultivation General Discussion [Re: Inocuole]
#27731354 - 04/12/22 12:27 PM (1 year, 9 months ago) |
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My petri cultures always sorta puff up when i open the lid. Wonder if it is from the temperature change, humidity change or CO2 level change. With that, i am wondering if I am choking my culture and perhaps crippling it in some way. I do use actual petris, but i wrap them like 3 times sometimes cause I am a freak.
I think a pretty big benefit of moding your pastys is that they are easier to open because the pressure can equalize.

Only pick i could find, but I think it is interesting that there is a very obvious line marking the time the lid got lifted.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 8 hours, 50 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27731370 - 04/12/22 12:41 PM (1 year, 9 months ago) |
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Quote:
p9hu7 said: The bottle that you pour agar from, like a media bottle?
No, I just loosen to pc.
Lol no, I meant pasty plates or however people call it these days.
Quote:
Dendrocopos said: I think a pretty big benefit of moding your pastys is that they are easier to open because the pressure can equalize.
Easier to open? Mine would literally crumble and get destroyed by the vacuum if I didnt loosen them a bit But yeah, that makes sense. I was asking about the fungus though, whether anyone sees any benefit to more gas exchange. Because mine do fine without any mods.
Edited by Adas (04/12/22 12:43 PM)
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Doctor Mario
πππππ



Registered: 08/07/20
Posts: 1,894
Loc: πΊπΈ
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Re: Cultivation General Discussion [Re: Adas] 1
#27731560 - 04/12/22 03:24 PM (1 year, 9 months ago) |
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Quote:
Adas said: I've never modified my plastic agar container lids, ever. Do you guys think the extra gas exchange is really beneficial?
Doubtful. My SSLC jars get closed up and completely sealed. Myc still colonizes the surface of the media in 7-10 days. I've wondered about this before but I haven't wanted to know bad enough to test it. Could make agar in some baby food jars and put transfers on each one. Obviously will wanna use clones of the same culture.
One jar completely sealed and another with GE holes. See how they perform against eachother.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 11 hours
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Re: Cultivation General Discussion [Re: Doctor Mario]
#27731562 - 04/12/22 03:30 PM (1 year, 9 months ago) |
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Here I can help actually, dr Mario. This information is only meant to cover cubes.
I ran jars without FAE for a period, to see the limits of it. You can do this normal for agar plates you want to grow out, reliably. They will have problems only long after the plate is grown out, and if you make a agar jar like that (sealed) you know its temporary media anyway.
I noticed that stuff that were choked out, would not usually recover in a transfer, it would just sit there, dead. (If kept sealed really long)
For grains (corn) the result was easy to guess. For bigger jars, completely sealed would stall out. For smaller jars, and fast moving strains, they would finish and could be spawned normally.
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (04/12/22 03:31 PM)
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