|
Ankhseramun
Wanderer



Registered: 10/11/21
Posts: 589
Loc: Cantina, Mos Eisley, Tatooine
Last seen: 1 year, 7 months
|
|
No need to really read anything, just take a shovel, find a good shady spot with not too much direct sunlight and bury the cakes and cover with something like 3-4 inches of earth/substrate (like a topping layer).
|
tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 36 minutes
|
|
With millet/birdseed I've been waiting two days on purpose to noc. I've found the sweaty conditions immediately after the PC'ing to cause less then perfect colonization. Anything other than going back to drying grains on window screens.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (04/11/22 04:51 AM)
|
polaritymind
relaxed attention


Registered: 10/10/16
Posts: 994
Loc: Germany
Last seen: 3 months, 9 days
|
|
Considering cubensis is a (sub?)tropical species, as the name says coming from cuba I think this would only work in summer. I tried it once, in my colder climate (germany) and it didnt work. And it wasnt even deep winter, but dont remember when exactly. Anyway even in the hottest summer its gets cold enough outside at night that its uncomfortable, all my attempts at sleeping under the open sky, even after scorching hot days were unsuccesfull. But I also really fucking hate the cold and didnt have a special sleeping bag or anything
-------------------- "to affirm life is to also affirm death" -Albert hofmann
|
Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
|
Re: Cultivation General Discussion [Re: Elf_on_a_Log] 2
#27729892 - 04/11/22 06:19 AM (1 year, 9 months ago) |
|
|
Quote:
Elf_on_a_Log said:
Quote:
WhiteThumbScience said: I already thirded it but absolutely they should never really contaminate in theory but practically a few years?
After a few years endospores would have germinated and contaminated them. Here's what happened to 21 untouched agar plates over 1.5 years. Approximately 15% of them survived contam-free. The rest were taken out by endospores.
https://www.shroomery.org/forums/showflat.php/Number/24453422#24453422
Endospores belong to bacteria, not mold. The odds of bacterial endospores being in your agar are slim to none. Endospores aren't ubiquitous, not all bacterium produce endospores nor are they present on every surface.
Your pasty plates were compromised in some way that allowed mold to ennter the cups. That does not look like bacterial growth.
|
mellow_meister


Registered: 03/12/22
Posts: 79
Last seen: 2 months, 4 days
|
Re: Cultivation General Discussion [Re: Inocuole]
#27730210 - 04/11/22 12:33 PM (1 year, 9 months ago) |
|
|
I used a white grocery bag and put near a window for some indirect light. Had good success.
|
wild_aussie
Punk rocker til I die



Registered: 01/05/22
Posts: 1,000
Loc: Skatepark grinding hard
Last seen: 6 days, 1 hour
|
Re: Cultivation General Discussion [Re: Inocuole]
#27730360 - 04/11/22 02:53 PM (1 year, 9 months ago) |
|
|
Just a quick question just to reassure what I already "think" I know. With brf cakes after harvested you should remove any pins, aborts, and anything else that's not part of the cake before you redunk it, is that correct? Also after first 24 hr dunk do the subsiquent dunks need 24 hrs or just 6-12 hrs
-------------------- BEWARE OF PICK POCKETS AND LOOSE WOMEN!! "Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!" Hunter S. Thompson "I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse " Sticking feathers up your ass does not make you a chicken!" Tyler Durden "He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson The noob forum Crack Plates Fastfreds media cookbook 60 agar/lc recipes Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide Trusted teks and linksSEED "PETRI" TEK Vent ur PC
|
Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 8 hours, 14 minutes
|
Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27730372 - 04/11/22 03:07 PM (1 year, 9 months ago) |
|
|
There's gonna be a ton in grain water agar, perhaps others. Not sure if thats what he used tho.
|
wild_aussie
Punk rocker til I die



Registered: 01/05/22
Posts: 1,000
Loc: Skatepark grinding hard
Last seen: 6 days, 1 hour
|
Re: Cultivation General Discussion [Re: Adas]
#27730395 - 04/11/22 03:27 PM (1 year, 9 months ago) |
|
|
Quote:
Adas said: There's gonna be a ton in grain water agar, perhaps others. Not sure if thats what he used tho.
If this is an answer to my question then I'm really confused but if for some other post then I'm sorry
-------------------- BEWARE OF PICK POCKETS AND LOOSE WOMEN!! "Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!" Hunter S. Thompson "I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse " Sticking feathers up your ass does not make you a chicken!" Tyler Durden "He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson The noob forum Crack Plates Fastfreds media cookbook 60 agar/lc recipes Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide Trusted teks and linksSEED "PETRI" TEK Vent ur PC
|
smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 10 hours
|
Re: Cultivation General Discussion [Re: Adas] 1
#27730400 - 04/11/22 03:32 PM (1 year, 9 months ago) |
|
|
preparing the text for the thread "Smalltalks ignorant pseudoaztecorum grow 2022"
since people have been so cool with sending me exotics, ive been working with pseudoaztecs again from a new print
previous experience with this was a 2021 jar that fruited 1 shroom and it had the pseudomonas
so not a super great grow. but got a nice culture finished on corn now
spawned them outside: pre colonized flower pots, grain straight to ground, shoeboxes to unheated inside.
my process now is to read up on the temperatures and the substrate, i love just doing agar work, taking to corn and GO.
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (04/11/22 03:33 PM)
|
Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
|
Re: Cultivation General Discussion [Re: Adas] 1
#27730407 - 04/11/22 03:39 PM (1 year, 9 months ago) |
|
|
Quote:
Adas said: There's gonna be a ton in grain water agar, perhaps others. Not sure if thats what he used tho.
You got me there, I did not think of that but you are absolutely right. I dislike grain water anything, probably why it slipped my mind
|
Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
|
Re: Cultivation General Discussion [Re: Adas]
#27730408 - 04/11/22 03:41 PM (1 year, 9 months ago) |
|
|
Quote:
Adas said: There's gonna be a ton in grain water agar, perhaps others. Not sure if thats what he used tho.
It was dehydrated malt extract agar purchased from out-grow.com, mixed with tap water, cooked at 15psi for 45 minutes, foil never removed once done, and placed in new ziplock bags right out of the pc. There's no way anything could have contaminated it. Whatever grew on the plates survived that extremely long pc process.
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
|
Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
|
Re: Cultivation General Discussion [Re: Elf_on_a_Log]
#27730434 - 04/11/22 04:04 PM (1 year, 9 months ago) |
|
|
I wouldn't be so sure of that, but that's all I have to say on the issue...no horse and all.
Two of a trade never agree, and these convos are a race to the bottom most of the time
|
Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
|
Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27730463 - 04/11/22 04:29 PM (1 year, 9 months ago) |
|
|
I'm set on figuring out the source of the contams. I've used that type of agar frequently for nearly a decade and rarely see a contam even with plates sitting for months sometimes. I didn't know endospores were only bacterial. Just went and learned why. A google search turned up pages of things about mold spores that can survive extreme heat, though I don't know how common those types of spores are. I intend to repeat the experiment at some point using more than one type of agar. I'd like to know details I didn't keep records of, such as how long it took for that stuff to show up on the plates.
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
|
Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
|
Re: Cultivation General Discussion [Re: Elf_on_a_Log] 2
#27730470 - 04/11/22 04:32 PM (1 year, 9 months ago) |
|
|
Those cups had mold. Pasty plates are not hermetically sealed, it's not much of a mystery
Mold is quite aggressive; even if it were possible for mold to survive the sterilization cycle it would have germinated and overrun your plates long before the ones in question showed signs of contamination.
Given enough time mold will find any weaknesses in your gas exchange system.

This is the reason for some of the plates showing contamination while the others didn't; not because you were graced with a rare species of thermophilic mold.
Edited by Stipe-n Cap (04/11/22 05:05 PM)
|
rcraft
Stranger
Registered: 02/06/22
Posts: 37
|
Re: Cultivation General Discussion [Re: Inocuole]
#27730505 - 04/11/22 04:54 PM (1 year, 9 months ago) |
|
|
First shoebox hitting a nice pinset (1 quart of MSS inoculated WBS with ~1:2 ratio of Coir - didn't have agar yet, next time!).
Very nice pinset coming along, just noticing some that seem to be growing again from their head! lol

Not entirely sure why it's happening, but doesn't seem to be a problem. I do worry that the pin growth has slowed down, they came out about 7 days ago.
|
BagOfDicks
Procrastinator



Registered: 12/11/21
Posts: 419
Loc: PNW
Last seen: 1 month, 17 days
|
Re: Cultivation General Discussion [Re: wild_aussie]
#27730526 - 04/11/22 05:14 PM (1 year, 9 months ago) |
|
|
Quote:
wild_aussie said: Just a quick question just to reassure what I already "think" I know. With brf cakes after harvested you should remove any pins, aborts, and anything else that's not part of the cake before you redunk it, is that correct? Also after first 24 hr dunk do the subsiquent dunks need 24 hrs or just 6-12 hrs
Yeah you should remove the growth and I'd just go for a 12 hour dunk.
--------------------
   🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
Huskies
Boop More Snoots



Registered: 03/22/16
Posts: 1,048
Last seen: 1 day, 4 hours
|
Re: Cultivation General Discussion [Re: BagOfDicks]
#27730561 - 04/11/22 05:45 PM (1 year, 9 months ago) |
|
|
So, what's the newest spiel on light for shroomies? Last I heard good at all stages, and you don't need much at all. Now, how low is too low?
My comedic USB LED desklight to rechargeable powerbar is a new low in lazyness, from not wanting to run a cable.
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
|
Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
|
Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27730586 - 04/11/22 06:09 PM (1 year, 9 months ago) |
|
|
There is logic in that so it makes sense. I still have a hard time buying that so many of them somehow got mold spores on them in still air through 7 layers of covering: ziplock bag, foil, 4 layers of paper towel, and 1 layer of micropore tape. There were likely some mold spores in the bag that got in as I was putting the plates in, but soon after it was sealed it was still air for 1.5 years. I'll do further (intentional) experiments and post the results in the future.
Assuming you're correct, my original point has been reinforced: On a long enough time scale sterile grain jars will contam. If not by endospores, by mold spores that somehow find their way in.
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
|
Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 7 months
|
Re: Cultivation General Discussion [Re: Inocuole] 1
#27730858 - 04/11/22 10:08 PM (1 year, 9 months ago) |
|
|
Maybe the mold started practicing quantum tunneling. Are you sure there's not part of the story you're leaving out?
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Cultivation General Discussion [Re: Elf_on_a_Log]
#27730870 - 04/11/22 10:27 PM (1 year, 9 months ago) |
|
|
Quote:
Elf_on_a_Log said: On a long enough time scale sterile grain jars will contam.
mine dry out before they contam from time.
in fact of all the reasons something tams out time is like the last thing id ever consider.
fix your filters mold got in the plates before you bagged them.
1 layer mp tape wont cut it try 3 and paper towels arnt fiters so of course mold goes thru that. easy fix
|
|