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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: mushboy]
#24166251 - 03/16/17 07:48 AM (6 years, 10 months ago) |
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oh yeah.. to keep on subject.
i was thinking about using a brand of bagged manure for some cakes. need to give pans another shot and i cant get zoodoo right now.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: mushboy]
#24166408 - 03/16/17 09:23 AM (6 years, 10 months ago) |
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Okay I don't know what's up anymore.. lol
I'm about to post my step by step sterile work 
I'm doing something wrong. But I cannot figure out what it is.
Agar to agar
Spray soapy water on all sides of sab and towel. Then unwrap petris outside of sab making sure they stay closed. Put gloves on while I'm wearing a long sleeve work shirt. Torch the blade of my xacto all metal #11. Put inside sab lay on rack (blade out) ISO hands this also allows the blade to cool slightly then slowly come back in. Grab the blade cool in agar then take the transfer. Recently I have had my receiving dishes upside no problems yet.
Agar to grain.
Soapy water sab. Etc. Torch the entire blade and while still hot cut the Petri in half (the media) Then scoop under and lift the entire puck up. Take the lid off the recieveing jar that's been wiped with ISO and drop the puck in.
G2g
Soapy water sab etc. Wipe all jars master and recieveing with ISO. Take the master lid off and put it to the side. Then while twisting the master pour into the recieveing jar then recap.
LL
PC water with my agar . Soapy water sab etc. Wipe entire jar down. Soak hands in ISO and break the seal on the jar. Replace lid. Torch entire blade and cut the Petri in half. Scoop and lift while taking the lid off the recieveing jar and dropping it in. Then the rest is like the g2g minus the twist I do.
What gives? My hands never go over open media/jars/water. I'll probably make a dam video later just so you can visually see what I'm doing. I don't know if it's my grain prep or what my plates seem clean.
I normally don't make long posts because English was my worst subject. If any clarification is needed let me know.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Inocuole]
#24166437 - 03/16/17 09:33 AM (6 years, 10 months ago) |
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What size jars are you using? What grains do you use, and how long do you pc them?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Regular mouth quart jars. Rye. 1 hour 50 mins at 16 psi
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (03/16/17 09:46 AM)
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Have any of you picked up one of the CD's on ebay that has a fuck ton of books on it? I snaged one for like $4-5 and it is supposed to have 120 shroom books on it. Most of the other say they come with ~40 books, so not really sure. Was just curious if any of you had picked one of these up, and if you got good info off of it.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Naw just here
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 15 hours, 57 minutes
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Re: Cultivation General Discussion [Re: van hatton]
#24166598 - 03/16/17 10:26 AM (6 years, 10 months ago) |
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Try EvilMushroom666 grain prep for rye and try skipping the LI. Just straight G2G. If that works...Go back to LI and see what happens... Not a scientific approach, but it might provide some useful information...
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Shadowboxing the apocalypse and wandering the land
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 15 hours, 57 minutes
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Re: Cultivation General Discussion [Re: Crispykoot]
#24166603 - 03/16/17 10:27 AM (6 years, 10 months ago) |
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Meant agar to grain, but you get the idea...
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Shadowboxing the apocalypse and wandering the land
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Crispykoot]
#24166645 - 03/16/17 10:44 AM (6 years, 10 months ago) |
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Rye prep is rinse. Then simmer for 45mins~ until a few popped grains. I usually shoot for under 20 popped grains per jar. Then I strain and mix grains around for about 30ish until no steam comes off. Then I load and PC. My grains don't usually contam before inoculation.
I have done the shake test recently and have been able to spot a few bacterial jars so far. But one that had a great recovery went green this morning all ten I could see the individual grains that were going bad and seemed to be from the master it's self.
Also have done tiger drops recently (I have a shit ton of clones to test) those seem good so I can rule out my agar being dirty.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (03/16/17 10:45 AM)
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Cultivation General Discussion [Re: van hatton]
#24166679 - 03/16/17 10:56 AM (6 years, 10 months ago) |
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Why do you unwrap your plates ourside the sab? Are you only flaming the blade? I flame the blade and two inches of the handle. Why do you set down a sterilized blade? Cool in agar instead.
I see a lot of problems. Your method is too complicated, you are taking too many steps
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Kenetic]
#24166844 - 03/16/17 11:47 AM (6 years, 10 months ago) |
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Like a dumbass, I opened my APE syringe not in a SAB. It was used and didn't have a needle, just a lid thjng. So I took it off, screwed an old needle thing on, flamed it, wiped plastic syringe part with ISO, and did my think. More than 1 drop came out, and it damn sure didn't land in the middle....
Any chance of growing something transferable given those variables?
I've never used syringe to agar before.
-------------------- KSSS And PE WBS.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Moabfighter]
#24166848 - 03/16/17 11:48 AM (6 years, 10 months ago) |
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Probably fine if the syringe is clean enough to begin with
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Moabfighter]
#24166854 - 03/16/17 11:52 AM (6 years, 10 months ago) |
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IMO depends on bacteria count in there. I have a few syringes from a non-sponsor, before I found you guys! I put a couple drops of their spores on agar and FUCK WHAT A BACTRIA BLOOM! Now I know why the cakes from their syringes sucked so bad, lol the ones in my sig! Now the Costa Rica that I got from one of our trusted sponsors, well I put a couple drops on a plat and never saw a thing. The non-sponsor ones I will have to throw or use antibiotic agar for the initial germination.
It can work well, or bite you in the ass, but better agar than cakes!
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: Cultivation General Discussion [Re: ComebackKid]
#24166886 - 03/16/17 12:07 PM (6 years, 10 months ago) |
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Quote:
ComebackKid said:
Quote:
PinPornProducer said: If you get repeated results next time with ms then it proves my theory to be correct. It's all about environment nothing else and clean spawn
FTFY 
That's what I'm hoping and it definitely makes sense in theory. I've had consistently good ms grows so far. I'd hate to chock it all up to beginners luck but even 2 grows wouldnt prove much. I plan to continue working with MS almost exclusively for the foreseeable future so maybe one day I'll hit that 10 tub benchmark
Yo glad I am on the same page with some other minds on here. I have been on the same grind. almost every huge ms grow i do with the environment as a control, they almost always perform within the same range and BE. they often look a lot alike too, which i normally wouldnt think. I am doing 20 ms grows each in 3 different environments. Pods, shoeboxes and monos. Bod really got me thinking to try and do a 6x6 W x L, and do different Heights. see at what point returns diminish. Been thinking mycelium is way more capable to move around its gases and liquids than i previously thought. I really want to do the controlled WxL and differing H test to see.
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Kenetic]
#24166890 - 03/16/17 12:09 PM (6 years, 10 months ago) |
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Quote:
Kenetic said: Why do you unwrap your plates ourside the sab? Are you only flaming the blade? I flame the blade and two inches of the handle. Why do you set down a sterilized blade? Cool in agar instead.
I see a lot of problems. Your method is too complicated, you are taking too many steps
I wrap and unwrap outside the sab as long as it doesn't open and your not blowing air at them not contams same concept as unmodified lids for jars. I set it down on a rack with anything flamed facing outward not touching anything. The only part of my xacto that touches the rack is where my hand is. While it's set there I ISO my hands then grab the blade and cool in agar takes like 5 seconds.
I choose not to ISO before torching because I lit my hands on fire a few times 
How is this too complicated its what every video/write-up has?
Mist soapy water flame sterilize cool transfer I just wrote out everything I do so that you can visualize how I do things. More the point of the "steps"
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (03/16/17 12:14 PM)
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Lobi]
#24167039 - 03/16/17 01:14 PM (6 years, 10 months ago) |
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So a few months back I took MS cakes and made some trays. I was very surprised that there was as much uniformity as there was. I had even taken that syringe, shot it into 50ml of sterile water so it would stretch farther. Perhaps it was the genetics, but some how I do not think so. This is because there is a lighter cap version that pops up occasionally. About same size, just lighter color, more light caramel. I am testing out a mini-mono of the 1st one I found now. Just got a print off the one that popped up today. For 3 inch tall it had a very meaty stem, almost solid through, and a bit thicker than the rest.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 15 hours, 57 minutes
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Re: Cultivation General Discussion [Re: Moabfighter]
#24167106 - 03/16/17 01:40 PM (6 years, 10 months ago) |
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Quote:
bodhisatta said: Probably fine if the syringe is clean enough to begin with
Quote:
Moabfighter said: Like a dumbass, I opened my APE syringe not in a SAB. It was used and didn't have a needle, just a lid thjng. So I took it off, screwed an old needle thing on, flamed it, wiped plastic syringe part with ISO, and did my think. More than 1 drop came out, and it damn sure didn't land in the middle....
Any chance of growing something transferable given those variables?
I've never used syringe to agar before.
That APE was very clean...You should be ok. I had a few plates with more than one drop because I melted the needle a bit...All plates had no contams. Cap was put on in front of the flow hood.
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Shadowboxing the apocalypse and wandering the land
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Crispykoot]
#24167150 - 03/16/17 02:02 PM (6 years, 10 months ago) |
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When you realize that your Honey LC was really just BAD hooch! The 1St one I ever made, I used my LC needle to transfer some heavily diluted premixed agar. Shot a couple syringes full into a 1/2pint pasty plate that had a SHIP. That one gave me my current 50% results. I believe it was because that had a plastic lid and I swirled, and probably knocked some contams down in. YES I AM NOW DOING G2G and masters LOL. I am still working LC on the side as a learning experience. This was my 2nd attempt. Used only honey and made a 500ml jar of it. Well it failed. I now have 2 LME jars going. I hope they are not hooch as well. LOL At least I got the masters from agar plates.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Unless you have a wild print I think you can pretty much expect uniformity from any MS variety.
Lobi I find my BE is all over the place with MS. I've had 1 dry oz 2 dry oz and everything in between per quart of spawn. What I have found is that mini monos will produce consistantly higher BE than standard tubs. (Thankyou pasty and muda for driving that point home)
Moab APE had to be the cleanest syringe I've ever used. 4 out of 5 plates showed clean growth right off the bat. You're going to be working with agar I wouldn't sweat it bro.
Van Hatton How long do you let the tub sit before working in it? Do you have drafts in your work room? Do you g2g? G2G is a real art. Definitely takes some finesse. Both times I've tried I've ended up with the mean green
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: ComebackKid]
#24167308 - 03/16/17 02:58 PM (6 years, 10 months ago) |
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Cbk I let it sit for 5-10 mins. I pour tons of plates with no problems. I used to do open air inoculation and g2gs but with great success idk how I did it for 2 years.
I also turn my heater and close all vents to stop drafts. I even use a 50 gallon sterilite container just for g2g
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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