Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: < First | < Back | 2692 | 2693 | 2694 | 2695 | 2696 | 2697 | 2698 | 2699 | 2700 | 2701 | 2702 | 2703 | 2704 | 2705 | 2706 | 2707 | 2708 | 2709 | 2710 | 2711 | 2712 | Next > | Last >
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Cultivation General Discussion [Re: Mr.Hyde]
    #27690846 - 03/11/22 10:29 AM (1 year, 10 months ago)

I mean idk why anyone would need stains in this hobby other than figuring out living and dead spores?
And even then it's not the biggest deal even when trying to isolate spores, the dead ones are just dead ha.


Extras: Filter Print Post Top
InvisibleMr.Hyde
Strange but not a stranger
 User Gallery


Registered: 02/24/17
Posts: 279
Loc: Secret Laboratory
Re: Cultivation General Discussion [Re: QM33] * 1
    #27690861 - 03/11/22 10:39 AM (1 year, 10 months ago)

For seeing hard to spot stuff, like clamp connections.

From the page I linked earlier:
Quote:

Chemicals, Reagents, and Stains

While water can be used to mount mycological specimens, features are often difficult to see without using stains and/or reagents that mycologists use. The bad news is, some of these chemicals can be very difficult to obtain (even for mycologists). At a minimum, you will probably need the chemicals below.

KOH (potassium hydroxide) is a strong base often used to study mushrooms. Although it is sometimes difficult to obtain, KOH can usually be purchased without too much difficulty. Several major online vendors have it available. KOH is used in a 2 percent aqueous solution as a mounting medium for microscopic examination of mushrooms. As a medium it often does a good job of clarifying mounts and making tissues and structures visible. It has its drawbacks (for example it tends to swell some structures) but it has been used for so long by mycologists that using it is necessary if one wants to compare data with their work.

Phloxine is a red stain, particularly good for making structures more visible. Phloxine is readily available from online retailers.

Melzer's reagent is an iodine-based stain regularly used in mycological microscope work to better see tissues and to determine whether spores and tissues are amyloid, inamyloid, or dextrinoid. It is unfortunately extremely difficult to obtain. Melzer's contains water, iodine, and potassium iodide, all of which are fairly easy to get hold of—but it also contains chloral hydrate, which is a controlled substance. Thus, you won't be able to buy it easily. Virtually your only option is to beg it from a professional mycologist. Even mycologists have difficulty obtaining Melzer's, however, and if the mycologist you know can't afford to provide you with some of her precious supply (or if you cannot find a mycologist), your last resort is to try explaining your situation to your doctor and getting a prescription for chloral hydrate (not likely; it's a date-rape drug) or for Melzer's reagent itself, which a compounding pharmacist could mix according to the formula below, and which your doctor would need to write on the prescription (still not very likely, but not unheard of).






--------------------


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Cultivation General Discussion [Re: Mr.Hyde]
    #27691012 - 03/11/22 12:16 PM (1 year, 10 months ago)

idk why you would need to identify structures beyond clamp connection, I'm sure there are reasons. Any ideas there?
And the only reason I would need to identify clamps of for monos. And I mean if you see more than one in your culture it's probably not a mono, and idk if you would need a stian for that.


Extras: Filter Print Post Top
InvisibleMr.Hyde
Strange but not a stranger
 User Gallery


Registered: 02/24/17
Posts: 279
Loc: Secret Laboratory
Re: Cultivation General Discussion [Re: QM33] * 5
    #27691031 - 03/11/22 12:33 PM (1 year, 10 months ago)

Sometimes stains and reagents are necessary to ID mushrooms.

Quote:

...to determine whether spores and tissues are amyloid, inamyloid, or dextrinoid.




Plus the micro-photography comes out more colorful and cool looking.

Some (stained) ascospores from one of Alan's microscopy lecture/workshops a few years ago.


--------------------


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Cultivation General Discussion [Re: Mr.Hyde] * 2
    #27691116 - 03/11/22 01:37 PM (1 year, 10 months ago)

Word. Thanks man!


Extras: Filter Print Post Top
Offlinerockyfungus
dirty
 User Gallery

Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 10 hours
Re: Cultivation General Discussion [Re: QM33] * 1
    #27691419 - 03/11/22 04:59 PM (1 year, 10 months ago)

Histology involves stains. What's the point of a microscope if you aren't going to use it properly.
Even contams need to be stained to be identified.
https://www.grepmed.com/images/11743/stains-fungal-microbiology-fungus-staining
Not that this has any use for us. But if you have a microscope I assume you're trying to visualize tissues that you can't. Stains make that shit pop by staining let's say the cell wall, or cytoplasm.

Would you prefer to only see the walls of the fungi or the whole shebang? Maybe you just want to see if those clamp connections actually anastomose.


Edited by rockyfungus (03/11/22 05:04 PM)


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Cultivation General Discussion [Re: rockyfungus] * 1
    #27691428 - 03/11/22 05:06 PM (1 year, 10 months ago)

Glad to have you smart guys with scopes and science knowledge around. Makes me strive to learn so I can poorly attempt to keep up. I got so tired of being ignorant of terminology recently. Pretty cool to try and obsorb the big words when I'm reading them instead of letting it go in one ear and out the other.

Now I know what a basidiocarp and hymenium is from like in my brain.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Extras: Filter Print Post Top
OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 1 year, 7 months
Re: Cultivation General Discussion [Re: sandman420]
    #27691446 - 03/11/22 05:27 PM (1 year, 10 months ago)

So ive been very successful with agar and shoeboxes, but failed miserably with G2G. Granted the place I lived in was naturally dirty and my new house is very clean.

I’m about to do G2G again and would like to get good enough at it, that it’s my main way to colonize jars. Any suggestions that you feel are very important regarding G2G?

Lastly, is there anything better than Iso to sanitize?


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Cultivation General Discussion [Re: CatsLoveHouseMusic] * 2
    #27691468 - 03/11/22 05:48 PM (1 year, 10 months ago)

Are you using a box or hood?

G2G is really hard to do right in a glove box situation.

It's tempting to overload the box and be reaching all over the place when you have a mess of jars to do.

Just go slower than you think you should, do a few at a time at most, take your time again, go slow, turn off vents, wear a mask.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Cultivation General Discussion [Re: rockyfungus]
    #27691482 - 03/11/22 06:06 PM (1 year, 10 months ago)

Quote:

Mr.Hyde said:
Sometimes stains and reagents are necessary to ID mushrooms.

Quote:

...to determine whether spores and tissues are amyloid, inamyloid, or dextrinoid.




Plus the micro-photography comes out more colorful and cool looking.

Some (stained) ascospores from one of Alan's microscopy lecture/workshops a few years ago.







Interesting because I can't find a definition for those first two words...
And the third one all o can find is this...
https://en.m.wiktionary.org/wiki/dextrinoid
And ide love some elaboration on that.

Now I'm confused because I thought you mean anueploidy, or polyploidr, or even monoploid or diploid. Because I figured it would be crazy if you could see chromosomes with a compound microscope ha. So now I'm confused. And make even I thought you meant like mono or dikaryotic but idk.

I suppose I should go read your link. I will in the AM probably.


Quote:

rockyfungus said:
Histology involves stains. What's the point of a microscope if you aren't going to use it properly.
Even contams need to be stained to be identified.
https://www.grepmed.com/images/11743/stains-fungal-microbiology-fungus-staining
Not that this has any use for us. But if you have a microscope I assume you're trying to visualize tissues that you can't. Stains make that shit pop by staining let's say the cell wall, or cytoplasm.

Would you prefer to only see the walls of the fungi or the whole shebang? Maybe you just want to see if those clamp connections actually anastomose.








Histology is the study of microbiology? The only thing I would need a microscope for is isolating monokaryotic mycelium, and I'm sure I can do this without stains. So your saying I'm using it improperly?
What are you using it for?

And I know some contains need to be stained to be identified, but I have more confidence that a culture is clean just by the appearance of the mycelium.
I suppose if I wanted to verify the culture was clean I would have to go through and stain every single part of it, and continually do that lol, I mean I could verify a culture to all hell just to contaminate it on the next transfer ha.

Not that what has any use for us? That link? What are you noting in the link, that bacterias need to be stained or something? Cause I know some do, but some don't.

And I've been able to view cell walls and nuclei completely fine by just adjusting the little light hole thing on the scope lol (whatever the fuck that thing is called) :facepalm:


And what do you mean anastomose? Anastomosis is the connection between the hyphae.



Now my understanding may be unclear hear but if anastomosis occurs a clamp connection will follow, unless it's monokaryotic mycelium as it has no nuclei to transfer.
When anastomosis happens the mycelium transfers nuclei via the clamp connection, ensuring a even division of new nuclei, so I could see anastomosis happening without clamp connection, but that would be in the rare event of a monokaryotic culture.


And I can see clamp connections at 1000x. If I'm trying to isolate a monokaryotic culture all I have to do is find a sample that doesn't contain any, pretty much sampling different cultures that would most likely very apperantly have them, they are easy to identify in large number on any small sample really. So if you didn't see like 1 or 2 right off the bat in a sample and looked more and didn't see any, bamo. Isolating spores is the hard part.


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


Extras: Filter Print Post Top
OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 1 year, 7 months
Re: Cultivation General Discussion [Re: sandman420]
    #27691492 - 03/11/22 06:12 PM (1 year, 10 months ago)

I currently only have a SAB, I’d like to have/build a flow hood but have been debating if they’re worth it. You think they are? I know there are teks to make them, what do you think minimum price would be to make a decent one? I have a decent in-line fan for growing cannabis, but think that’s the wrong kind.

I have a 114 liter SAB (it’s pretty big.)


Extras: Filter Print Post Top
OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house Flag
Last seen: 3 hours, 24 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: CatsLoveHouseMusic] * 1
    #27691495 - 03/11/22 06:16 PM (1 year, 10 months ago)

:nonoyes:
FUCK YEAH
2x2 FFU on the zon for less than 6


--------------------

Crack's Tek's and Shit



Edited by Crackatoa (03/11/22 06:17 PM)


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 4 hours, 42 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: Crackatoa] * 1
    #27691499 - 03/11/22 06:19 PM (1 year, 10 months ago)

For g2g make sure to wipe down the colonized jar really well since you’ll be tilting it over an open sterile jar.


When I did g2g in a sab I used a balled up piece of foil or something that I could lean the receiving jar against at an angle. That way I had both hands and more height clearance for the jar I was pouring out of.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleMr.Hyde
Strange but not a stranger
 User Gallery


Registered: 02/24/17
Posts: 279
Loc: Secret Laboratory
Re: Cultivation General Discussion [Re: A.k.a]
    #27691519 - 03/11/22 06:40 PM (1 year, 10 months ago)

Quote:

QM33 said:

Interesting because I can't find a definition for those first two words...
And the third one all o can find is this...
https://en.m.wiktionary.org/wiki/dextrinoid
And ide love some elaboration on that.

Now I'm confused because I thought you mean anueploidy, or polyploidr, or even monoploid or diploid. Because I figured it would be crazy if you could see chromosomes with a compound microscope ha. So now I'm confused. And make even I thought you meant like mono or dikaryotic but idk.

I suppose I should go read your link. I will in the AM probably.





Color change reactions on exposure to iodine.
Used in wild mushroom identification.


Quote:

https://en.wikipedia.org/wiki/Amyloid_(mycology)
In mycology a tissue or feature is said to be amyloid if it has a positive amyloid reaction when subjected to a crude chemical test using iodine as an ingredient of either Melzer's reagent or Lugol's solution, producing a blue to blue-black staining.
Negative reactions, called inamyloid or nonamyloid, are for structures that remain pale yellow-brown or clear.
A reaction producing a deep reddish to reddish-brown staining is either termed a dextrinoid reaction (pseudoamyloid is a synonym) or a hemiamyloid reaction.




--------------------


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Cultivation General Discussion [Re: A.k.a]
    #27691520 - 03/11/22 06:40 PM (1 year, 10 months ago)



--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Cultivation General Discussion [Re: Mr.Hyde]
    #27691521 - 03/11/22 06:42 PM (1 year, 10 months ago)

Quote:

Mr.Hyde said:
Quote:

QM33 said:

Interesting because I can't find a definition for those first two words...
And the third one all o can find is this...
https://en.m.wiktionary.org/wiki/dextrinoid
And ide love some elaboration on that.

Now I'm confused because I thought you mean anueploidy, or polyploidr, or even monoploid or diploid. Because I figured it would be crazy if you could see chromosomes with a compound microscope ha. So now I'm confused. And make even I thought you meant like mono or dikaryotic but idk.

I suppose I should go read your link. I will in the AM probably.





Color change reactions on exposure to iodine.
Used in wild mushroom identification.


Quote:

https://en.wikipedia.org/wiki/Amyloid_(mycology)
In mycology a tissue or feature is said to be amyloid if it has a positive amyloid reaction when subjected to a crude chemical test using iodine as an ingredient of either Melzer's reagent or Lugol's solution, producing a blue to blue-black staining.
Negative reactions, called inamyloid or nonamyloid, are for structures that remain pale yellow-brown or clear.
A reaction producing a deep reddish to reddish-brown staining is either termed a dextrinoid reaction (pseudoamyloid is a synonym) or a hemiamyloid reaction.








Word idk why I couldn't find that on Google.. thank you! Much love


Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: Cultivation General Discussion [Re: QM33] * 2
    #27691569 - 03/11/22 07:12 PM (1 year, 10 months ago)

Quote:

QM33 said:
And by transmitted light microscope I assume you mean a regular compound



I hardly know anything about microscopes it was gifted to me by a friend. When I searched it online it said this kind was called 'tramsitted light microscope' here's a pic of mine



--------------------
Cakes inside Water Tub


Extras: Filter Print Post Top
Offlinerockyfungus
dirty
 User Gallery

Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 10 hours
Re: Cultivation General Discussion [Re: QM33]
    #27691674 - 03/11/22 08:33 PM (1 year, 10 months ago)

QM, not gonna quote.

Histology is observing anatomy with a microscope. What are you using a microscope for.

I don't own a microscope. There's no need. If I had one I'd be sure to stain. I don't know how to explain it but it's basic cable vs HD. Is it needed no. Will you go back? Probably not.

If ya got toys might as well use it to it's full advantage. Oil immersion...


It's just easier on the eyes...


Edited by rockyfungus (03/11/22 08:43 PM)


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada Flag
Trusted Cultivator
Re: Cultivation General Discussion [Re: rockyfungus] * 5
    #27691727 - 03/11/22 09:26 PM (1 year, 10 months ago)

Staining is common practice, it's how microscopy works. I can't see what QM33 is saying but I'm sure it's ridiculous. Stains are used for contrast, contrast sucks, why bother lol.


Extras: Filter Print Post Top
OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: Stipe-n Cap] * 1
    #27691755 - 03/11/22 09:44 PM (1 year, 10 months ago)

Quote:

p9hu7 said:
Staining is common practice, it's how microscopy works. I can't see what QM33 is saying but I'm sure it's ridiculous. Stains are used for contrast, contrast sucks, why bother lol.



Thanks for the reminder, p9, about ignoring unpleasant members. i really can’t stand constant arguments and high levels of aggression.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 2692 | 2693 | 2694 | 2695 | 2696 | 2697 | 2698 | 2699 | 2700 | 2701 | 2702 | 2703 | 2704 | 2705 | 2706 | 2707 | 2708 | 2709 | 2710 | 2711 | 2712 | Next > | Last >

Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* alt.drugs.mushrooms.cultivation (good/bad idea) BikeCourier 6,440 9 10/26/21 06:04 PM
by LogicaL Chaos
* Vegetative growth VS generative growth? LoveLightPeace 1,542 1 06/04/18 02:15 PM
by jason9086
* casing from "The mushroom Cultivator" upupup 17,893 8 11/13/20 10:41 AM
by tiptrippy
* YoshiTrainer's guide to low-prep grains
( 1 2 3 4 ... 12 13 all )
YoshiTrainer 8,237 249 01/07/24 06:48 PM
by MoJim
* Important - All Cultivators please read.
( 1 2 3 4 5 6 all )
ThorA 41,584 103 05/31/05 03:35 AM
by Anno
* Post deleted by users_request
( 1 2 3 4 5 all )
McMan 25,295 93 05/06/01 01:56 PM
by 3DSHROOM
* Post deleted by users_request
( 1 2 3 4 5 all )
NuShroomPharmerII 26,676 85 06/04/01 04:53 PM
by MNmyc
* VOTE!! Outdoor Cultivation Forum w/Moe For Mod!!
( 1 2 3 4 all )
TM 23,449 73 04/13/10 01:30 AM
by jingus

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,194,934 topic views. 35 members, 319 guests and 78 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.046 seconds spending 0.026 seconds on 15 queries.