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SpaceBaby
alchemist



Registered: 11/01/20
Posts: 2,030
Loc: SPACE
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Re: Cultivation General Discussion [Re: Phrontist]
#27676933 - 02/28/22 01:18 PM (1 year, 10 months ago) |
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nice work
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Inthepit] 1
#27677136 - 02/28/22 04:37 PM (1 year, 10 months ago) |
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Quote:
Inthepit said:
Quote:
Mateja said: Anything less than 2% nutrients in agar would result in the colony visibly struggling.
Well shoot, all my agars have been under 2%, but I have started adding yeast. 6g 1.3% Agar, 6g LME, 1g Yeast, 450ml Blu
So it looks like I need 8g LME + 1g yeast = 9g in 450ml = 2% nutrients
(it's 450ml because I use a 500ml bottled water, simmer and strain it. Which brings it down to 450ml )
Actually I'm prolly losing a lot of the yeast by straining, so perhaps that lessens the nutes.
Oki oki, so do you measure the amount of media left after boiling or are you just guestimating that 50ml has boiled off? If so then your nutrient density is around 1.55%.
I did some testing when I first started with agar and when I compared 1% and 2% MEA I noticed that my cultures looked stronger, healthier and grew considerably faster on higher percentage. Here's a few side by sides I did with 2 different clones
 
 
And this is the same test but with a multistrain culture
 
Obviously the multistrain culture didn't show as much difference in growth as the clones did, and perhaps I should have chosen a different word than 'struggling'. Have you done any comparisons yourself to see if your cultures can grow more thicker/faster on a more nutritious media?
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Inthepit
Aum Mani Padme Hum


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 2 hours
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Re: Cultivation General Discussion [Re: Mateja]
#27677209 - 02/28/22 05:41 PM (1 year, 10 months ago) |
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Thankyou Meteja, I appreciate how hard your working to set the tek straight.
So I'm using graduated media bottles and they end up at 450ml. And YES the plates on the left are exactly my experience!
I'm eager to try a more nutritious agar recipie. It will be awhile however, we're selling the house and moving to a different country.
Also I read that a rolling boil for Brewers yeast and just using the broth will have plenty of the nutrients.
Edited by Inthepit (02/28/22 05:52 PM)
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: Mateja]
#27677217 - 02/28/22 05:47 PM (1 year, 10 months ago) |
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Quote:
Mateja said:
Quote:
Inthepit said:
Quote:
Mateja said: Anything less than 2% nutrients in agar would result in the colony visibly struggling.
Well shoot, all my agars have been under 2%, but I have started adding yeast. 6g 1.3% Agar, 6g LME, 1g Yeast, 450ml Blu
So it looks like I need 8g LME + 1g yeast = 9g in 450ml = 2% nutrients
(it's 450ml because I use a 500ml bottled water, simmer and strain it. Which brings it down to 450ml )
Actually I'm prolly losing a lot of the yeast by straining, so perhaps that lessens the nutes.
Oki oki, so do you measure the amount of media left after boiling or are you just guestimating that 50ml has boiled off? If so then your nutrient density is around 1.55%.
I did some testing when I first started with agar and when I compared 1% and 2% MEA I noticed that my cultures looked stronger, healthier and grew considerably faster on higher percentage. Here's a few side by sides I did with 2 different clones
 
 
And this is the same test but with a multistrain culture
 
Obviously the multistrain culture didn't show as much difference in growth as the clones did, and perhaps I should have chosen a different word than 'struggling'. Have you done any comparisons yourself to see if your cultures can grow more thicker/faster on a more nutritious media?
Was the clone/culture originally grown on 2% agar? Could be an adaptive trait.? Maybe even a strain previously grown similarly before the genetics for to you. Interesting to see the ms less reactive.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Inthepit]
#27677237 - 02/28/22 05:58 PM (1 year, 10 months ago) |
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Quote:
Inthepit said: Thankyou Meteja, I appreciate how hard your working to set the tek straight.
So I'm using graduated media bottles and they end up at 450ml. And YES the plates on the left are exactly my experience!
I'm eager to try a more nutritious agar recipie. It will be awhile however, we're selling the house and moving to a different country.
Also I read that a rolling boil for Brewers yeast and just using the broth will have plenty of the nutrients.
That's awsome! (unless you're moving from Ukraine lol) I also just moved a few weeks ago and actually today got the stove installed so I can finally get back to growing 
Quote:
QM33 said: Was the clone/culture originally grown on 2% agar? Could be an adaptive trait.? Maybe even a strain previously grown similarly before the genetics for to you. Interesting to see the ms less reactive.
Hmm that's actually a very good point, haven't though about that, it's definitely a possibility. Tho I don't find it very plausible since the medium doesn't change and the nutrient type doesn't change so there shouldn't be much of any 'adapting' going on but we won't know for sure until some side by sides are made, and I have to cook agar tomorrow anyway so
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: Mateja] 1
#27677252 - 02/28/22 06:06 PM (1 year, 10 months ago) |
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Well it's a nutrient density variable, and how the mycelium may be reacting, and adapting genetically to it.
Like another example might be, I think certain strains simply perform better on master's mix. Because they were bred on it, and commercially produced on it for years after before I got the culture or spores from a prior leneage and put them in wheat. And maybe not only because of things like say nitrogen but also like cellulose content. And we know mycelium exchanges genetic information. So say you take either of those MS cultures and test test a sector vice versa, and with a control, and compare might be an experiment worthy of the topic. And although you only learn so much from growing something on any certain medium, it might suggest something. Kind of like what your doing.
Although it is interesting you see no difference in LC at variablized nute rates but you do with agar, any theories as to why this might be???
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Mateja]
#27677254 - 02/28/22 06:08 PM (1 year, 10 months ago) |
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Here's the same clone2# culture on 2% and 4% BRF agar. I'm not sure where it came from, most likely from a 2% MEA plate. The 4% BRF had noticeably thicker and faster growing culture than 2%. I don't even if this says anything really regarding 'adaptibility' right now I think I should probably try with even more nutritious recipes just to see what's what.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: QM33]
#27677260 - 02/28/22 06:12 PM (1 year, 10 months ago) |
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Quote:
QM33 said: Although it is interesting you see no difference in LC at variablized nute rates but you do with agar, any theories as to why this might be???
Idk but considering how much bigger the volume of the available nutrient media is in liquid then one can assume this is why myc doesn't mind lower nutrient levels when they're so readily available. Could be something else also but it's clear that you'd never get away with that on a solid media.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Mateja]
#27677272 - 02/28/22 06:31 PM (1 year, 10 months ago) |
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I dont think 4% brf is the same thing as 4% sugar
BRF is probably mostly carbs ehh?
Yea BRF has 65 times more carbs than s ugar
only 0.7 grams of sugar in 195 grams of Brown Rice
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: sandman420]
#27677277 - 02/28/22 06:42 PM (1 year, 10 months ago) |
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They're actually quite similar, only real difference is BRF has a lot more nutritious carbohydrates (from human perspective)
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Mateja] 1
#27677286 - 02/28/22 06:54 PM (1 year, 10 months ago) |
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What are you talking about one has 71 grams of sugar and one has 0,7 grams of sugar lmao
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 10 hours
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Re: Cultivation General Discussion [Re: Mateja]
#27677292 - 02/28/22 06:59 PM (1 year, 10 months ago) |
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What's fiber and fat do? I know for humans it slows down the uptake of sugar. Fucking labels. One is complex sugar vs simple sugar or however you want to say. Has to effect how quickly myc can digest.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: sandman420]
#27677293 - 02/28/22 07:00 PM (1 year, 10 months ago) |
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I'm talking about how they have different kinds of carbohydrates, one of them having the much more nutrient ones than the other (from a human perspective) One has 76g and the other has 71g, as I said very similar. 316 vs 363 calories.
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Edited by Mateja (02/28/22 07:04 PM)
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: sandman420]
#27677310 - 02/28/22 07:12 PM (1 year, 10 months ago) |
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Quote:
sandman420 said: What are you talking about one has 71 grams of sugar and one has 0,7 grams of sugar lmao
Sugar is a simple carbohydrate and what’s listed as carbohydrates are complex carbohydrates. Our bodies aren’t super great at digesting complex but good at simple, hence sugar rushes but also crashes.
Fungi are capable of digesting both, and will more readily digest simples but might gain more benefit from the complex, but that’s outside my expertise, in fact most of what I said is, but that’s my 
Also hi mateah, good to see you on this board
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Wall.E]
#27677320 - 02/28/22 07:20 PM (1 year, 10 months ago) |
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 3 hours, 18 minutes
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Re: Cultivation General Discussion [Re: Mateja]
#27677332 - 02/28/22 07:30 PM (1 year, 10 months ago) |
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I'm will Wall-E, Good to see you Mateja, It's been awhile I feel. Hope you've been well.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: Crackatoa]
#27677343 - 02/28/22 07:37 PM (1 year, 10 months ago) |
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Hi Will, nice to meet you
-------------------- Life’s shit, but I’m loving it
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Crackatoa]
#27677344 - 02/28/22 07:37 PM (1 year, 10 months ago) |
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And congrats on the tag btw there are so many new blackbelts I barely have track on all the new, it's quite nice to see sheriff's all over the place
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Edited by Mateja (02/28/22 07:38 PM)
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 3 hours, 18 minutes
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Re: Cultivation General Discussion [Re: Wall.E]
#27677350 - 02/28/22 07:40 PM (1 year, 10 months ago) |
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I noticed my punctuation was horrible and didn't even notice that shit. I'm leaving it too
I could have sworn you had a badge as well. I would have lost money on that bet. Glad to hear you got your stove and going to have you back.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Crackatoa]
#27677359 - 02/28/22 07:46 PM (1 year, 10 months ago) |
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Oh please no badge for me lol I couldn't do that to management, at least not yet, lemmenfirst fpgure out how to make my posting 'easier on the eyes' before I start representing more than just myself. I'm not Dr.Anthony Fauci now am I?
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