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InvisibleMateja
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Re: Cultivation General Discussion [Re: sandman420]
    #27675907 - 02/27/22 06:06 PM (1 year, 10 months ago)

I also notice a considerable slowdown after half of the solution has been colonized, but if I stop swirling and let them go on they seem to always end up colonizing fully and then fruit formation starts on the surface.


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InvisibleQM33
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Re: Cultivation General Discussion [Re: Mateja]
    #27675919 - 02/27/22 06:20 PM (1 year, 10 months ago)

Quote:

Forrester said:
Quote:

QM33 said:
All you have to do is read a little bit on endospores and thermolific bacterias and you would know that's is simply not true.
Read the links. If you even understood how low acid canning worked you would know that's not true.




:whathesaid:

Quote:

natedawgnow said:
@forrester-Steam sterilization works just fine and steam is the same temp as boiling water so i disagree with the assertion that boiling water doesnt sterilize.




Disagree all you want, but do some research, check those links and find out why you're wrong then you can agree :wink:





Quote:

QM33 said:

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/geobacillus-stearothermophilus

https://ispe.org/pharmaceutical-engineering/november-december-2013/steam-sterilization-principles-common-mistakes

https://www.britannica.com/topic/food-preservation/Sterilization

Might need an account for the last one










So you don't have to go all the way back

And here's another one
"Boiling water kills or inactivates viruses, bacteria, protozoa and other pathogens by using heat to damage structural components and disrupt essential life processes (e.g. denature proteins). Boiling is not sterilization and is more accurately characterized as pasteurization. Sterilization kills all the organisms present, while pasteurization kills those organisms that can cause harm to humans.
https://www.health.ny.gov/environmental/water/drinking/boilwater/response_information_public_health_professional.html

PrimalSoup said:
Quote:

natedawgnow said:
By definition steam sterilization works at atmospheric pressure with temps at 212 degrees, same as boiling water.






No it doesn't.  https://www.cdc.gov/infectioncontrol/guidelines/disinfection/sterilization/steam.html

Quote:


The basic principle of steam sterilization, as accomplished in an autoclave, is to expose each item to direct steam contact at the required temperature and pressure for the specified time. Thus, there are four parameters of steam sterilization: steam, pressure, temperature, and time. The ideal steam for sterilization is dry saturated steam and entrained water (dryness fraction ≥97%).813, 819 Pressure serves as a means to obtain the high temperatures necessary to quickly kill microorganisms. Specific temperatures must be obtained to ensure the microbicidal activity. The two common steam-sterilizing temperatures are 121°C (250°F) and 132°C (270°F). These temperatures (and other high temperatures)830 must be maintained for a minimal time to kill microorganisms. Recognized minimum exposure periods for sterilization of wrapped healthcare supplies are 30 minutes at 121°C (250°F) in a gravity displacement sterilizer or 4 minutes at 132°C (270°F) in a prevacuum sterilizer (Table 7). At constant temperatures, sterilization times vary depending on the type of item (e.g., metal versus rubber, plastic, items with lumens), whether the item is wrapped or unwrapped, and the sterilizer type.








Quote:

sandman420 said:
Yes water is what the end result of my argument lies but you were using atmospheric sterilization as a point which my retort is that doesn't exist.

The facts are that boiling water kills MOST germs. That's the end of the argument. You may or may not have issues it's a big wide world with a lot of water.











Mateja I don't think


Quote:

QM33 said:
I think ...





Qualifies as

Quote:

Mateja said:
making 'matter of fact statements' about stuff..




And I can't disagree with your experiment.

But check this out, you think the myc would take up the whole jar if you tried them on bigger jars? I use mostly 4 oz jars now and the grow always fills the jar, this wasn't usually the case with quart jars, idk I feel like something might be at play their. OP to the question had a seemingly big jar. Idk. No hard shit in that one, just a I think
.


Not numerous scientific links from governmental and educational foundations, like how 212F wet heat won't sterilize anything.


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InvisibleThe Fresh PrintsS
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Re: Cultivation General Discussion [Re: QM33] * 3
    #27675931 - 02/27/22 06:31 PM (1 year, 10 months ago)

:notyou:


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InvisibleMateja
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Re: Cultivation General Discussion [Re: QM33]
    #27675939 - 02/27/22 06:39 PM (1 year, 10 months ago)

Quote:

QM33 said:
I think it really depends on how much nutrients you put in it. Like if you put little to none in you could expect little to no mycelium.



This doesn't sound like you're wildly speculating, it sound more like you're trying to explain what happens if you put "little" nutrients in a solution. And I'm telling you first off you're obviously wrong but what's important is someone will read that and think you know what you're talking about and then they'll quote you on and on and years later I'll still be here annoying the crap out of you and everyone else that takes it personally... But at least you can trust what I say on these boards...

:likeaboss::havesomescience:


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Edited by Mateja (02/27/22 06:41 PM)


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Re: Cultivation General Discussion [Re: The Fresh Prints] * 1
    #27675941 - 02/27/22 06:39 PM (1 year, 10 months ago)

I got nothing to add. Shit ain't sterile anywhere if I want to be devils advocate.
We reach satisfactory levels of sterilization for our small hobby. Scaling up is where it bites you in the ass fast.

Pendantics come get me...


Edited by rockyfungus (02/27/22 06:46 PM)


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Invisiblebigfootscreepyuncl
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Re: Cultivation General Discussion [Re: The Fresh Prints]
    #27675944 - 02/27/22 06:40 PM (1 year, 10 months ago)

:migraine:


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: bigfootscreepyuncl] * 1
    #27675947 - 02/27/22 06:41 PM (1 year, 10 months ago)

Sunday Steamery Nights are now a thing


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Re: Cultivation General Discussion [Re: sandman420] * 1
    #27675954 - 02/27/22 06:46 PM (1 year, 10 months ago)

That like a Cleveland steamer?


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InvisibleQM33
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Re: Cultivation General Discussion [Re: bigfootscreepyuncl]
    #27675957 - 02/27/22 06:47 PM (1 year, 10 months ago)

4 hours ago...

Quote:

sandman420 said:
That's the end of the argument.




....


PW9ine and fresh princess- :baby:


Sadly mateja as cool as you think your experiment is I think that as the media volume increases the need for the mycelium to expand does not, and gas exchange may be other variables to consider, as well as nute source, variety and strain. So I see you, but it's not a game changer in my book.

Quote:

bigfootscreepyuncl said:
:migraine:





#steamey


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: QM33]
    #27675974 - 02/27/22 06:57 PM (1 year, 10 months ago)

Hey one of you brainiacs want to come over and say something about genes/isolation on this thread  How can tissue produce a 1:1 clone, but spores express different xenotypes?

My understanding is that you can't really isolate genetics on a clone much/any further because the mating has already been established in the hyphal network. So no way to get back to anything called an isolate/pure strain. Whadaya think.


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InvisibleMateja
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Re: Cultivation General Discussion [Re: QM33]
    #27675981 - 02/27/22 07:00 PM (1 year, 10 months ago)

Quote:

QM33 said:
Sadly mateja as cool as you think your experiment is I think that as the media volume incr.....



That's the difference between us, you "think" you know stuff and I know from experience, you keep pulling stuff out of your magic hat and I keep doing my 'sad' due diligence as an attempt to raise the overall knowledge of this place, I didn't come here to flood this place with assumptions and speculations, rather I'm trying to rid this place of those. And don't take anything personal, but if you keep saying stuff that doesn't make any sense imma keep questioning those statements and those are the rules I play by. Didn't come here to win popularity contests either, came here to learn and to spread KNOWLEDGE. :mushroom:


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: Mateja]
    #27675983 - 02/27/22 07:02 PM (1 year, 10 months ago)

had this q out there all day in the contam threa. maybe you guys can answer...

help, please

i have black slimy areas scattered throughout the fruits from my huatla harvest this morning.

do i pink though the whole thing and toss the black slimy bits
or do i toss the harvest
wouldn't be the end of the world. i have a fuckton. 



kept 150g tossed 70.


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InvisibleQM33
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Re: Cultivation General Discussion [Re: Mateja]
    #27675985 - 02/27/22 07:02 PM (1 year, 10 months ago)

I appreciate your efforts.

See cited professional research based information links above for KNOWLEDGE.

Keep up the experiments, I'm sure you'll figure something out.


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Re: Cultivation General Discussion [Re: QM33]
    #27675993 - 02/27/22 07:05 PM (1 year, 10 months ago)

That is Pseudomonas (bacteria blotch)

It's not ideal...Throw it out. If there are any non slimey bits should be "safe" as long as you don't have aids


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Edited by sandman420 (02/27/22 07:11 PM)


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InvisibleMateja
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Re: Cultivation General Discussion [Re: QM33]
    #27676007 - 02/27/22 07:11 PM (1 year, 10 months ago)

Quote:

QM33 said:
Keep up the experiments, I'm sure you'll figure something out.



Like I've been doing since I joined? Ok boss whatever you say boss
:buzzaldrin:


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: sandman420]
    #27676019 - 02/27/22 07:18 PM (1 year, 10 months ago)

Quote:

sandman420 said:
That is Pseudomonas (bacteria blotch)

It's not ideal...Throw it out. If there are any non slimey bits should be "safe" as long as you don't have aids




thank you. :bow2:

that is what i did. really wanted that pseudomonas name.
Lymphoma yes, AIDS no. TFG.


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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27676020 - 02/27/22 07:18 PM (1 year, 10 months ago)

Don't fuck around with Pseudomonas or any contams with compromised immune system!
https://doi.org/10.1093/infdis/130.Supplement.S24


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Re: Cultivation General Discussion [Re: rockyfungus] * 1
    #27676025 - 02/27/22 07:22 PM (1 year, 10 months ago)

Quote:

rockyfungus said:
Don't fuck around with Pseudomonas or any contams with compromised immune system!




yikes, cool. i scratched my eyeball earlier this year. couldn't go swimming cause doc said pseudomonas in pools, hottubs. said eyeball is toast if infected. i got through.

luckily, have had great quarterly blood tests; only 'immuno-compromised' when i want a noVid booster ahead of y'all.

EDIT: wikipedia info

Pseudomonas

Pseudomonas is a genus of Gram-negative, Gammaproteobacteria, belonging to the family Pseudomonadaceae and containing 191 validly described species. The members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a wide range of niches. Wikipedia

Specific to cult:

Although not strictly a plant pathogen, P. tolaasii can be a major agricultural problem, as it can cause bacterial blotch of cultivated mushrooms.[30] Similarly, P. agarici can cause drippy gill in cultivated mushrooms.


Edited by SpaceBaby (02/27/22 07:33 PM)


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Invisiblebigfootscreepyuncl
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Re: Cultivation General Discussion [Re: QM33]
    #27676035 - 02/27/22 07:26 PM (1 year, 10 months ago)

@SpaceBaby - that shit looks nasty lol. No idea what it is though so I'm not much help. Shocker, I know :lol:



When using a grocery sack as a shoebox liner do I have to worry about the cleanliness of the bag or just get to it? They're not visibly dirty but have been used. I'd rather not have to wipe them out or anything but if that's SOP I guess that's what I gotta do!


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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27676040 - 02/27/22 07:29 PM (1 year, 10 months ago)



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