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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Crackatoa]
    #27671985 - 02/24/22 05:11 PM (1 year, 10 months ago)

The new ones should come sterile.


When I sterilize used syringes they just barely fit in the instant pot. I used to just cram them in but a couple started getting bent tips so I had to switch to taking the needles off and screwing plugs in.


It’s a little tedious but at least you don’t have to worry about water boiling out that way.


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LAGM2020


Edited by A.k.a (02/24/22 05:14 PM)


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: A.k.a]
    #27672028 - 02/24/22 05:41 PM (1 year, 10 months ago)

That's what I thought, I'm not a syringe user but I'm starting some Pans :mushroom2:


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Invisiblejoze
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Re: Cultivation General Discussion [Re: joze] * 1
    #27672068 - 02/24/22 06:01 PM (1 year, 10 months ago)

Quote:

joze said:
I had three jars of colonized grains ready to spawn and no coir to spawn it to. I went to three different pet stores and they were all sold out of coco fiber, so I ended up getting Exo Terra "coco husk," which is the same stuff it's just a chunkier texture. I pasteurized it and set up my shoeboxes, and honestly I'm pretty unhappy with it, but it'll do for this round.
It definitely doesn't hold as much water as the coco fiber, I did my best to really compress it and I think I'll still get a decent canopy. Fingers crossed.




This coco husk is fucking me. It colonized really quick, honestly. I got some nice rhizomorphic growth with quick recovery. But the surface conditions are awful. Getting some bruising and yellowing of the mycelium. For some reason I can't keep it moist, even though I'm getting side pins. I managed to find a store with Eco Earth in stock today, so I'm currently pasteurizing a mini batch of coco coir and I'm going to case these shoeboxes with maybe a half inch of moist coir. I'm hoping that they'll recover and I'll be able to get a decent flush...



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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: Forrester] * 2
    #27672362 - 02/24/22 09:54 PM (1 year, 10 months ago)

Quote:

Forrester said:
How are you sterilizing the water by just boiling it?




Boiling kills everything in the water.  It works.. ppl have been doing it for years.  You don’t need to pc water to sterilize it (I guess technically you do to call it sterilized)

15 minutes is probably even overkill. Apparently you can make sterile even boiling for a minute but I like overkill


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OfflineForresterM
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Re: Cultivation General Discussion [Re: fahtster] * 1
    #27672565 - 02/25/22 02:45 AM (1 year, 10 months ago)

Huh! :themoreyouknow:

Always thought some bacteria could survive boiling water...

But I guess it's just the spores of some like clostridium and bacillus.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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InvisibleQM33
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Re: Cultivation General Discussion [Re: Forrester] * 1
    #27672656 - 02/25/22 05:54 AM (1 year, 10 months ago)

Not just spores of bacteria but thermolific bacterias like Bacillus stearothermophilus in a non spore state will survive and even grow in temperatures up to like 220. But then ya endospores.


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: QM33]
    #27672716 - 02/25/22 07:15 AM (1 year, 10 months ago)

Well now im unsure.. maybe I’ve just been getting lucky this whole time.. I guess it can be a risk but I wouldn’t have grown anything in the last 3 months if it didn’t work.. and like I said ppl have been doing it for years using drinking water. 
Fack.. now I have to buy better syringes.  Thought I had it figured out where I could reuse these non-pcable ones


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: fahtster]
    #27672760 - 02/25/22 08:12 AM (1 year, 10 months ago)

Humans have been boiling river water to make it potable for a thousand years.

It works


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InvisibleQM33
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Re: Cultivation General Discussion [Re: fahtster]
    #27672763 - 02/25/22 08:14 AM (1 year, 10 months ago)

There's alot of things that work. And I'm not even trying to put that in a way like your doing it wrong. But I think all to often people get TOO technical, it clearly works. We're farming right? If it works grow it!


And it would make since for endorspores not to be in most water, and bacterias like geothermal stereobaci whatever, are very uncommon, and usually only found in hot water springs, and probably aren't in our water supplies or soils or anything I would assume, but are the standard for sterilization.

With that said idk if there is appropriate research done on simply boiling or steaming said bacteria. I would like to get biological indicator strips for 212F trials but they're expensive. Going to make a listing in the marketplace for anyone who wants to throw in eventually.



I'm sure certain people have gotten very sick from simply boiled water also. And there's a big difference between potable, consumable for the human body, and in use for sterile procedure, and then what you can get away with in mushroom cultivation probably ha


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          there are also unknown unknowns.
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InvisibleQM33
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Re: Cultivation General Discussion [Re: QM33]
    #27672768 - 02/25/22 08:18 AM (1 year, 10 months ago)

If you think about it, along with thermal dynamics and heat transfer, that's what people are doing to sawdust blocks, even heavily supped mastermix which has got to be comparable I'm bioburden endospores as grains. People steam medias all the time. Between bioburden, vigor of fungi and SAL ide bet more appropriate means of understanding these things will develop, because clearly mediums need not be completely sterile to grow on.


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: QM33]
    #27672776 - 02/25/22 08:27 AM (1 year, 10 months ago)

And I’ve always pc’d syringes before I got these shitty ones.. it was just convenient to throw them in with the grain jars and I’ve read the sucking up thing years ago and then again recently and it’s been working but I don’t want “it’ll probably work”. I just didn’t look into it enough until y’all pointed out it can’t be classified as sterile.  I want sterile.  Fack.. if it isn’t one thing it’s another, I’m tellin ya. 

I always thought if the syringe said bd it wasn’t going to get mangled in the pc.. not true folks.. at least not filled.  I’m going to try foiled and empty.. maybe at least I can sterilize them and fill from a jar of pc’d water


Edited by fahtster (02/25/22 08:31 AM)


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InvisibleMTZ
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Re: Cultivation General Discussion [Re: fahtster]
    #27672784 - 02/25/22 08:35 AM (1 year, 10 months ago)

Interesting. Never been a fan of BD. I go through a LOT of barrels because of medical shit. I much prefer Nipro. I wonder how they hold up? I get the by the 100ct. So it's no biggie to just grab a box 9f the 5 or 10ml barrela. I gotta fire up the PC today. Maybe I'll toss one in to see. If it doesn't stand up. It's only like $20 for 100 so not life changing if it doesn't.


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Rehears your death every morning and night. Only when you constantly live as though already a corpse will you find freedom in the martial way, and fulfill your duties without fault throughout your life. ~ The Book Of Samurai
........
I live in the void. I can go to the in between anytime I desire. I visit the spirt world.


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InvisibleQM33
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Re: Cultivation General Discussion [Re: fahtster]
    #27672787 - 02/25/22 08:37 AM (1 year, 10 months ago)

I put syringes in a jar with 2 sfds, you could use tyvek, no lid. Allowing the steam to penetrate the jar as much as possible while keeping it sterile after the cycle, like a sterilization pouch. Then recently I started keeping around a jar of sterilized water with a ship and a syringe filter on it so I can suck it out without a vacuum forming. I wrap the rings with gladwrap.
Also to go along with your ship sterilization method, I thought about it in regards to my sterile water jar, and I was even thinking about putting said jar in a grow bag then sterilizing then only opening the bag in sterile conditions and retrieving the water through the ship, to keep the shop truly sterile.
What's BD? 8 wouldn't trust anything that's not PP
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/geobacillus-stearothermophilus

https://ispe.org/pharmaceutical-engineering/november-december-2013/steam-sterilization-principles-common-mistakes

https://www.britannica.com/topic/food-preservation/Sterilization

Might need an account for the last one


Edited by QM33 (02/25/22 08:47 AM)


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: QM33]
    #27672798 - 02/25/22 08:51 AM (1 year, 10 months ago)

It’s just the brand name Beckton dickinson  .. it was a stupid correlation I made because all the syringes I got from vendors were bd and you could pc them over and over np.. and maybe they use to be .  Could be that the syringes I bought from the medical supply place are a knock off.. idk.  Or there’s just different grades of syringes that I was unaware of.. probably that, but the place only had one option for 10ml bd syringes so I thought it was all good


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InvisibleQM33
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Re: Cultivation General Discussion [Re: fahtster]
    #27672806 - 02/25/22 09:03 AM (1 year, 10 months ago)

Fuck. Ya I put some pippetts in with my syringes like a week ago, they all melted lol, idk what I thought ha, lol they're still in with the syringes too I haven't got them out haha


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InvisibleE_Dubbya
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Re: Cultivation General Discussion [Re: QM33]
    #27672812 - 02/25/22 09:08 AM (1 year, 10 months ago)

I put a bunch of BD syringes in the PC, some melted and some didn't, so you may be on to something in regards to there being different versions or knock offs.


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Edited by E_Dubbya (02/25/22 10:18 AM)


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: QM33]
    #27672815 - 02/25/22 09:10 AM (1 year, 10 months ago)

Faht have you modified your cooker to run over 15 psi?

Usually the stoppers are rubber which is stable up to ~253f

Quote:

West typically recommends autoclaving: (a) a steam cycle at a minimum temperature of 121.1°C (not to exceed 123°C) for a maximum of 60 minutes




This is for rubber containing particles from here https://www.westpharma.com/blog/2017/december/best-practices-in-sterilization-of-rubber-articles#

Some syringes may have TPE (thermoplastic elastomer) stoppers which could be or could not be steam sterilization safe depending on the type.

Personally I've never seen a stopper melt or anything and used a lot of BD syringes. Although I haven't touched a syringe in at least 2 years.

Actually I take that back...I have totally melted syringes. When I ran out of water I ruined a AA925 with 40 melted syringes stuck. Wanna see a crazy pic?


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: sandman420]
    #27672839 - 02/25/22 09:43 AM (1 year, 10 months ago)

Yeah.. it’s not the rubber, it’s the plastic plunger and needle “knob” get all bent to shit when there’s any kind of force put on them (like a full syringe leaning at an angle).. there’s different numbers on the plunger for the ones that work that are from SW.  I’m at work but I’ll post a pic later tonight


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: QM33]
    #27672850 - 02/25/22 09:56 AM (1 year, 10 months ago)

Quote:

QM33 said:
I would like to get biological indicator strips for 212F trials but they're expensive.




Yo Q would AUTOCLAVE TAPE be a good indicator tool?


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InvisibleQM33
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27672874 - 02/25/22 10:17 AM (1 year, 10 months ago)

It's not the same. Bio strips contain geobacillus stearothermophilus the most thermo resistant bacteria on the planet, and maybe even one of the oldest life forms in existence.

Autoclave tape changes over at a certain temperature.

I don't think enough studies have been done with 212f steam treatments on geobacillus stearothermophilus personally, or bacterias, but I'm probably wrong. But again SAL vs practicality may find a more appropriate balance in the myco world in the future


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


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