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Prakriti
Strange

Registered: 12/06/21
Posts: 84
Last seen: 10 months, 17 days
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Re: Cultivation General Discussion [Re: Crackatoa]
#27670748 - 02/23/22 06:44 PM (1 year, 10 months ago) |
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👍 gratzy!
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AphexPin
Strange

Registered: 02/22/22
Posts: 464
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Re: Cultivation General Discussion [Re: Crispykoot]
#27670801 - 02/23/22 07:30 PM (1 year, 10 months ago) |
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Quote:
Crispykoot said: Hey all. Quick question. Who's got the best foggers right now?
If you want the best, Aeromist.
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SpaceBaby
alchemist



Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
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Re: Cultivation General Discussion [Re: Nerex]
#27670887 - 02/23/22 08:45 PM (1 year, 10 months ago) |
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Quote:
Nerex said:
Quote:
Drboomer said:
Quote:
Nerex said: In order to do a syringe of mycelium water from a PF Tek cake (pint), will 10 mls of sterile water be enough? I mean, will I be able to retrive all those 10 mls of water back in the syringe from the jar?
Also, this has to be done in the SAB?
This is called a grain liquid culture or glc. In order to get 10 mls back you would have to inject much more than that. Yes it must be done in a SAB.
This technique isn't used much anymore because if you inoculate your pf Tek cake with spores there is a 99.99% chance it contains bacteria. This will turn your syringe into gross bacterial soup
OK, thank you!
So I'm gonna go with the spore syringe method, but I have a question about it... Not having a PC, would it be sufficient to just sanitize the shot glass with alcohol 70%? If not, it would be enough for sterilization to put it in a oven at 266°F for just 20 minutes? If possible, I'd avoid to have it in the oven for an hour or more.
get a PC
first, buid an SAB
no you can't sterliize with ISO it disinfects
you really should read the 'start here' threads at the minimum. many of these and similar questions are answered therein.
-------------------- SpaceBaby SPACEBABY'S LAGM22 THREAD MUSHBOY'S SHROOM TEA TEK Me as a cube
Another Day's Work:
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ozbuckley
Stranger
Registered: 09/09/10
Posts: 57
Last seen: 1 year, 20 days
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Re: Cultivation General Discussion [Re: SpaceBaby]
#27670906 - 02/23/22 09:02 PM (1 year, 10 months ago) |
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Hi
First monotub grow here. Using Bods tek. With the lid on my tub upside down, it leaves two gaps like this

Is that gap (x2) enough for air circulation?
Thanks
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SpaceBaby
alchemist



Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
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Re: Cultivation General Discussion [Re: ozbuckley]
#27670919 - 02/23/22 09:14 PM (1 year, 10 months ago) |
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Quote:
ozbuckley said: Hi
First monotub grow here. Using Bods tek. With the lid on my tub upside down, it leaves two gaps like this

Is that gap (x2) enough for air circulation?
Thanks
good. that's for your FAE and is why the TEK instructs lid upside down.
-------------------- SpaceBaby SPACEBABY'S LAGM22 THREAD MUSHBOY'S SHROOM TEA TEK Me as a cube
Another Day's Work:
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 4 hours, 29 minutes
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Re: Cultivation General Discussion [Re: ozbuckley]
#27670923 - 02/23/22 09:20 PM (1 year, 10 months ago) |
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Quote:
ozbuckley said: Hi
First monotub grow here. Using Bods tek. With the lid on my tub upside down, it leaves two gaps like this

Is that gap (x2) enough for air circulation?
Thanks
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ozbuckley
Stranger
Registered: 09/09/10
Posts: 57
Last seen: 1 year, 20 days
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Re: Cultivation General Discussion [Re: Crackatoa]
#27670985 - 02/23/22 10:31 PM (1 year, 10 months ago) |
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I just PC sterilized a batch of jars with grains.
My LC to inoculate them in a week or so away (my stupid ass made this mistake).
Obviously the grains are sterilized so there should no issue with them sitting out until I can add LC. but should I keep the lid as normal so the polyfil hole in the lid can exchange air/gas, or should i wrap the lid in plastic wrap to seal it off completely?
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
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Re: Cultivation General Discussion [Re: ozbuckley]
#27671029 - 02/23/22 11:21 PM (1 year, 10 months ago) |
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I leave the foil on mine till they're ready. No need to plastic wrap
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ozbuckley
Stranger
Registered: 09/09/10
Posts: 57
Last seen: 1 year, 20 days
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Re: Cultivation General Discussion [Re: Crackatoa]
#27671042 - 02/23/22 11:46 PM (1 year, 10 months ago) |
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Quote:
Crackatoa said: I leave the foil on mine till they're ready. No need to plastic wrap
Thanks!
Another quick one: Im making a jar of LC from a LC syringe. Can I reuse this now empty syringe from a vendor who I received the original LC (in the syringe) from?
Flame sterilizing the needle of course, is there any other danger to sucking up the new LC from the jar into the re-used syringe?
thanks
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SpaceBaby
alchemist



Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
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Re: Cultivation General Discussion [Re: Crackatoa]
#27671044 - 02/23/22 11:48 PM (1 year, 10 months ago) |
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I just keep them like that. I recently took a couple weeks to nocc like 70 jars. Now I have been using them faster so, great, I am the PC’s bitch. 
—
i am trying to understand that question just now.
-------------------- SpaceBaby SPACEBABY'S LAGM22 THREAD MUSHBOY'S SHROOM TEA TEK Me as a cube
Another Day's Work:
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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Re: Cultivation General Discussion [Re: SpaceBaby]
#27671075 - 02/24/22 12:37 AM (1 year, 10 months ago) |
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I reuse my syringes. I just suck up and squirt out boiling water through them a couple times. You could also PC it if needed.
-------------------- Two eyes to look, One eye to see. Prying open my third eye 
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schpat
psychesomadelic



Registered: 09/26/21
Posts: 1,039
Loc: South Africa
Last seen: 10 days, 11 hours
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I'd spawn it, i'd spawn it hard.

gooodbye hooorsessss
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 20 hours
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Re: Cultivation General Discussion [Re: ozbuckley]
#27671122 - 02/24/22 02:18 AM (1 year, 10 months ago) |
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Quote:
ozbuckley said:
Quote:
Crackatoa said: I leave the foil on mine till they're ready. No need to plastic wrap
Thanks!
Another quick one: Im making a jar of LC from a LC syringe. Can I reuse this now empty syringe from a vendor who I received the original LC (in the syringe) from?
Flame sterilizing the needle of course, is there any other danger to sucking up the new LC from the jar into the re-used syringe?
thanks
You can definitely reuse the syringe, but PC it wrapped in foil and don't open till you're gonna use, inside SAB. I wouldn't just do the sucking up boiling water method.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: Forrester] 1
#27671288 - 02/24/22 07:12 AM (1 year, 10 months ago) |
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Ya gotta be careful tho with some syringes.. if it’s from a vendor, it might be ok in the pc.. I bought a box of bd syringes from a medical supply place and the plunger won’t survive the pc. So to reuse them, I steam them in a vegetable steamer for a half hour (plunger and cap separated from barrel) and then do the boiling water (boil the water for 15+ minutes first) and suck up the boiling water (letting it sit in the syringe for a minute each time) and squirt back into the boiling water and repeat… on the 4th time, I leave the water in the syringe.. leave the syringes to cool sitting straight up in a cup or they can end up with a huge air bubble occasionally. Works great
Edit: While this does work and has been working, technically you need to pc the water and syringes to classify them as sterile.. while you probably won’t have organisms that can survive 212F in your water and used syringes, there’s still a chance that something could fuck up your grow doing it ^^^that way. Just heads up
If the syringe is new and in the package, obviously, you don’t have to use the vegetable steamer.
Faht
Edited by fahtster (02/25/22 08:36 AM)
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 4 hours, 29 minutes
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Re: Cultivation General Discussion [Re: fahtster]
#27671503 - 02/24/22 10:39 AM (1 year, 10 months ago) |
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Do you still do the boiling water with a brand new syringe? I gotta go buy some today from TSC
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: Crackatoa]
#27671924 - 02/24/22 04:04 PM (1 year, 10 months ago) |
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Yeah.. gotta sterilize the water. Unless I’m not understanding what you’re askin
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 20 hours
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Re: Cultivation General Discussion [Re: fahtster]
#27671937 - 02/24/22 04:20 PM (1 year, 10 months ago) |
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How are you sterilizing the water by just boiling it?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 4 hours, 29 minutes
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Re: Cultivation General Discussion [Re: Forrester]
#27671959 - 02/24/22 04:41 PM (1 year, 10 months ago) |
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If I buy new syringes to draw up LC would you do the water thing first or go straight to sucking.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: Crackatoa] 1
#27671961 - 02/24/22 04:43 PM (1 year, 10 months ago) |
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Straight to sucking you say
-------------------- Life’s shit, but I’m loving it
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 4 hours, 29 minutes
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Re: Cultivation General Discussion [Re: Wall.E] 2
#27671982 - 02/24/22 05:08 PM (1 year, 10 months ago) |
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 I don't get to use that first one enough
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