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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 11 hours
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Re: Cultivation General Discussion [Re: SwabMarley]
#27647069 - 02/05/22 02:17 PM (1 year, 11 months ago) |
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Myc Hunt said:
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DERRAYLD said: 300ml I wouldn't use more than 0.3g.
So 0.1%? Sounds about right. I’ll be cooking that shit up tomorrow then and let you know how it goes. Will be adding a stir bar and stirring at fuck knows what RPM at fuck knows what intervals
I just made some agar LC earlier this week, 0.2% nutes 0.125% agar (500mL water, 1g LME, 0.5g agar), and it seems really thick to me! Even with a stir bar, you have to crank that shit up 
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bigfootscreepyuncl said:
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ozbuckley said: 1. Can quart jars, specifically the lids with a hole in them, be re-used over and over again?
The jars and lids can be re-used multiple times. I don't have a number for you but it's a lot of times.
Kind of a shot in the dark but an decent educated guess, i've probably PC'd mine at least 18-20 times and i just had my first lid get bad enough that i need to throw it out.
I've been using the same lids since i started and i'm just now starting to have lids get trashed enough that i need to replace them, so we're talking two full years of almost back to back usage. There was one point where they got rusty enough that i took some steel wool to clean them up which helps extend the life. I use vinegar in the water and since i built my PID on it, i think that may be accelerating the rust by having aluminum+brass in contact. I have teflon tape on the fitting but still think it might be having an effect.
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ozbuckley
Stranger
Registered: 09/09/10
Posts: 57
Last seen: 1 year, 20 days
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Quote:
MysticMycologist said: Can you post a pic? It shouldn’t be too wet. Make sure your grains are dry on the outside but plump and hydrated before loading them. Sometimes they can be a little wet looking right after PCing but just shake it and it should re-absorb when it cools. The grains should shake easily and not be all stuck together or stuck to the glass. I usually dry my rye grains for a couple hours on a screen in front of a fan before loading. Sometimes mine look a little too dry, but those jars seem to preform better than the wetter looking ones.
Sure. Here are two pics

The is no water pooling at the bottom of the jars. Just condensation on the walls. The grains are loose and not stuck together at all, however the occasional grain sticks to the wall of the jar due to the condensation.
Hopefully this is all ok 🤞
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 20 hours
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Re: Cultivation General Discussion [Re: ozbuckley]
#27647233 - 02/05/22 04:48 PM (1 year, 11 months ago) |
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Sometimes if I'm lazy and don't steam dry enough before loading and my grains come out of the PC a bit too wet, I just let em sit a few days or a week and the extra moisture escapes through the GE port. Of course I use like a 1/2" ge hole and climate is real dry here...
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 10 hours, 54 minutes
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Re: Cultivation General Discussion [Re: Forrester]
#27647328 - 02/05/22 05:44 PM (1 year, 11 months ago) |
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If the contents are moist while sterile and your innoculant axenic then it's all good
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 41 minutes
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Re: Cultivation General Discussion [Re: Forrester]
#27647331 - 02/05/22 05:45 PM (1 year, 11 months ago) |
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Quote:
Forrester said: Sometimes if I'm lazy and don't steam dry enough before loading and my grains come out of the PC a bit too wet, I just let em sit a few days or a week and the extra moisture escapes through the GE port. Of course I use like a 1/2" ge hole and climate is real dry here...
Depending on how the grains were hydrated, they may still have some water holding capabilities... so some of that might be going into the inner seed.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 20 hours
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Re: Cultivation General Discussion [Re: tedoro]
#27647342 - 02/05/22 05:51 PM (1 year, 11 months ago) |
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Quote:
tedoro said:
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Forrester said: Sometimes if I'm lazy and don't steam dry enough before loading and my grains come out of the PC a bit too wet, I just let em sit a few days or a week and the extra moisture escapes through the GE port. Of course I use like a 1/2" ge hole and climate is real dry here...
Depending on how the grains were hydrated, they may still have some water holding capabilities... so some of that might be going into the inner seed.
Absolutely! I'm sure it's a combination of the grains reabsorbing the moisture and some getting lost through the ge hole. I just know some does get lost through my holes, in the winter here, cause they'll get REAL dry if I leave em too long.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Phrontist
Determined



Registered: 07/17/13
Posts: 518
Last seen: 7 months, 12 days
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Quote:
bigfootscreepyuncl said: The last gummies I made I used the entire fruit (sporulating caps included) and couldn't taste any mushroom at all and I cooked 56g down into 4oz of concentrated tea.
That's cute. I'm starting with 800g of mushrooms and cooking down to 80ml of extract liquid. Try those numbers and tell me if taste still isn't an issue. That's not to mention the "sludge" that forms when all the solid-ish material (like spores) is condensed that far. My recipe calls for 80ml of water... not 20ml of water and 60ml of sludge.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 10 hours
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Re: Cultivation General Discussion [Re: Forrester]
#27647357 - 02/05/22 06:03 PM (1 year, 11 months ago) |
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So when I get dry uncolonized kernels in my jars, top, it's because of too big filter?
(Assuming normal prep, not dry)
Beeb seeing more of those in winter which I guess is drier as well? Why are winters drier airwise: what dictates what
-------------------- Willpower is the one true virtue
  
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 5 hours, 19 minutes
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Yeah if you cut down the GE the tops will colonize.
I never had fully colonized top grains for over a year until I made new lids with tiny holes. Not that it makes a difference though.
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LAGM2020     
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: Cultivation General Discussion [Re: Phrontist] 3
#27647386 - 02/05/22 06:24 PM (1 year, 11 months ago) |
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Phrontist said:
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bigfootscreepyuncl said: The last gummies I made I used the entire fruit (sporulating caps included) and couldn't taste any mushroom at all and I cooked 56g down into 4oz of concentrated tea.
That's cute. I'm starting with 800g of mushrooms and cooking down to 80ml of extract liquid. Try those numbers and tell me if taste still isn't an issue. That's not to mention the "sludge" that forms when all the solid-ish material (like spores) is condensed that far. My recipe calls for 80ml of water... not 20ml of water and 60ml of sludge.
cool story bro. I'm not sure who pissed in your cheerios but it's clear now that you can't be bothered with people attempting to help - that was rude of me to waste your time. Carry on
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I 5318008 NOT a virgin!
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 10 hours, 54 minutes
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They make fancy equipment for extractions. Maybe stop wasting caps and get some glassware? I'm feelin me a rotary evap...
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: Cultivation General Discussion [Re: Phrontist] 1
#27647596 - 02/05/22 08:37 PM (1 year, 11 months ago) |
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Phrontist said: I’m only interested in extraction for gummies at this point
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Phrontist said: That’s cute
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shrams
Stranger

Registered: 01/14/22
Posts: 10
Last seen: 1 year, 9 months
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Re: Cultivation General Discussion [Re: Inocuole]
#27647615 - 02/05/22 08:47 PM (1 year, 11 months ago) |
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A question for you more experienced growers out there: How should I progress on my first grow from agar to grain?
I'm new to cultivation, and after a lot of reading over the past few months I decided to buy a spore syringe and inoculate some agar plates. I now have some successful germination on the agar, and I've completed my first transfer.


My question is this: should I keep transferring until I have a monoculture? Or, should I just take one of these colonized agar plates with multiple strains on it and transfer it to grain? This way, I can wait to see what fruits are produced and clone the best ones.
I'm worried that if I go through the trouble of transferring and isolating on agar before a full grow, I might end up with a subpar culture. Thoughts?
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: Cultivation General Discussion [Re: shrams] 2
#27647625 - 02/05/22 08:52 PM (1 year, 11 months ago) |
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Quote:
shrams said: My question is this: should I keep transferring until I have a monoculture?
Just transfer until the is culture clean enough for grain inoculation.
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shrams
Stranger

Registered: 01/14/22
Posts: 10
Last seen: 1 year, 9 months
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Re: Cultivation General Discussion [Re: ModularMind]
#27647628 - 02/05/22 08:57 PM (1 year, 11 months ago) |
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ModularMind said:
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shrams said: My question is this: should I keep transferring until I have a monoculture?
Just transfer until the is culture clean enough for grain inoculation.
Thanks for the advice!
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Phrontist
Determined



Registered: 07/17/13
Posts: 518
Last seen: 7 months, 12 days
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Quote:
bigfootscreepyuncl said:
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Phrontist said:
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bigfootscreepyuncl said: The last gummies I made I used the entire fruit (sporulating caps included) and couldn't taste any mushroom at all and I cooked 56g down into 4oz of concentrated tea.
That's cute. I'm starting with 800g of mushrooms and cooking down to 80ml of extract liquid. Try those numbers and tell me if taste still isn't an issue. That's not to mention the "sludge" that forms when all the solid-ish material (like spores) is condensed that far. My recipe calls for 80ml of water... not 20ml of water and 60ml of sludge.
cool story bro. I'm not sure who pissed in your cheerios but it's clear now that you can't be bothered with people attempting to help - that was rude of me to waste your time. Carry on
Nah, I asked to be pointed towards psil producers that have more non-cap material than average. You told me about how your gummies don't taste bad to you. I'm struggling to see how it's even related, let alone helpful, but go ahead and get your feelings hurt, I guess.
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Phrontist
Determined



Registered: 07/17/13
Posts: 518
Last seen: 7 months, 12 days
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Re: Cultivation General Discussion [Re: ModularMind]
#27647695 - 02/05/22 10:01 PM (1 year, 11 months ago) |
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Quote:
ModularMind said:
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Phrontist said: I’m only interested in extraction for gummies at this point
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Phrontist said: That’s cute

So many feelings up in here lately. I asked a simple question: which psil producers have more non-stem material than most? I didn't ask for a lecture on what individuals feel is wasteful. I didn't ask for personal opinions about what I'm up to. Reason being, this is a faceless internet forum, and we don't REALLY care about each other.
If you actually give a shit, I take psil for its mental health benefits first and foremost. Unfortunately, in the past few years, my body has developed a severe aversion to the mushroom material, to the point where I will vomit on ingestion, and be physically ill for a full 24 hours after a dosage. This is after decades of eating them by the handful without giving a moment's thought to the taste. So yeah, my personal project has become figuring out a way to get psil into my body, without feeling like my abdomen is going to burst, all so I don't commit suicide.
There you go, my good, close, personal friend who truly and deeply gives a shit about me. Or were you just looking to be clever with an assumption that I'm a party-boy who likes to trip in between Jell-O shots?
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: Cultivation General Discussion [Re: Phrontist] 3
#27647751 - 02/05/22 11:13 PM (1 year, 11 months ago) |
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Phrontist said:go ahead and get your feelings hurt, I guess.
No, my feelings aren't hurt even in the slightest. In order for a person to hurt my feelings I have to respect them, and my respect is one of the many things in life you'll never get because you have to give it in order to get it. But I get the feeling you've heard that more than once in your life so I'll spare that dead horse an extra kicking.
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Phrontist said:So many feelings up in here lately. I asked a simple question: which psil producers have more non-stem material than most? I didn't ask for a lecture on what individuals feel is wasteful. I didn't ask for personal opinions about what I'm up to. Reason being, this is a faceless internet forum, and we don't REALLY care about each other.
It actually sounds like you're the one getting your huggies in a wad, big guy. Not one person was lecturing, you got plenty of opinions on which 'psil producers' have more *non-cap* material (nice proof reading there, pal) and like you just said, this is a faceless internet forum and nobody gives a flying fuck what you're up to.
If you actually give a shit, I take psil for its mental health benefits first and foremost. Unfortunately, in the past few years, my body has developed a severe aversion to the mushroom material, to the point where I will vomit on ingestion, and be physically ill for a full 24 hours after a dosage. This is after decades of eating them by the handful without giving a moment's thought to the taste. So yeah, my personal project has become figuring out a way to get psil into my body, without feeling like my abdomen is going to burst, all so I don't commit suicide.
Did you forget your rant in the last half-assed paragraph; going on and on about how we don't actually give a shit about each other? Because once I finished reading this paragraph all I could think about is that there is any number of members on here that I would actually be distraught about if anything were to ever happen to them. You are not one of them and I couldn't give a shit less about your sensitive tummy because you don't know how to take care of yourself mentally or physically.
There you go, my good, close, personal friend who truly and deeply gives a shit about me. Or were you just looking to be clever with an assumption that I'm a party-boy who likes to trip in between Jell-O shots?
Now that that's out of the way, you've got me wondering which aspect of your avatar you actually think you uphold:

It can't possibly be 'respect'. It's not 'research' because you would then start looking into more efficient methods of extraction and/or the cultivation of species that have higher content of actives - or you would have simply come across the Melmaks and PEs and had your incredibly vague question answered. Unless you finally 'realize' that you're an insufferable asshole that's not it either and it certainly isn't the recycle emblem because you're so fucking wasteful.
You thought you hurt my feelings but it's the exact opposite. Dealing with people like you always makes me happy that I pulled my head out of my ass 20 years ago, so I actually should be thanking you. Best of luck with your extraction and crummy tummy, now kindly get bent.
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I 5318008 NOT a virgin!
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Phrontist
Determined



Registered: 07/17/13
Posts: 518
Last seen: 7 months, 12 days
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Quote:
bigfootscreepyuncl said: nobody gives a flying fuck what you're up to.
Yet here you are writing an essay, dissecting my avatar, and even spellchecking my posts. I'm sorry I pointed out that your anecdote was completely irrelevant. Find a shoulder to cry on and maybe you'll get over it someday, sweetie.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 10 hours
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Re: Cultivation General Discussion [Re: Phrontist]
#27647851 - 02/06/22 02:34 AM (1 year, 11 months ago) |
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-------------------- Willpower is the one true virtue
  
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