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Invisiblemistamonsta
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Registered: 07/06/11
Posts: 888
Loc: Uranus
Re: Cultivation General Discussion [Re: Markamello]
    #27608706 - 01/06/22 06:21 AM (2 years, 23 days ago)

@ Markamello I thought from your post that it was just affecting colonisation and fruiting time, not increased comtam
You are absolutely right about contams thriving in higher temperatures.
There's also the factor of mycelium being stressed at higher temperatures and struggling to resist or out compete the contams.
But in saying that the contamination still has to come from somewhere :shrug:
I'd reason that it has to be slipping through in one of your workflow, sterilisation or pasteurisation processes somehow and the increased temp is just letting it take hold quicker and easier....


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MistaMonsta's LAGM 2022


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Offlinerockyfungus
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Re: Cultivation General Discussion [Re: Markamello]
    #27608718 - 01/06/22 06:35 AM (2 years, 23 days ago)

Quote:

Markamello said:
This is my first time culting through a summer. Do consistent temps over 80f cause the setbacks that I'm facing? It feels like I've got no idea that I'm doing again.





Welcome to an issue I feel most of us experience after culting for a moment. It's the great cultivation slap to the face. It took me 6 months in the summer to remedy my own issues in a very arid area. I'm extremely lucky with my climate and still had to take a break and really deep clean my laboratory.

Hate to say it but a fresh coat of paint and really cleaning up the space goes a long way.

Lots of people just close shop til cooler weather. If you're driven it's a great way to see where the process is failing. You're own your own probably troubleshooting, unfortunately.

Maybe someone can watch your tek?


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OfflineMarkamello
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27608757 - 01/06/22 07:28 AM (2 years, 23 days ago)

Zeems I could be forgetting cult 101, contams comes from dodgy spawn.

The warmer weather could be accentuating a flaw in my technique.

I recently moved house too. My old lab was in a garage with concrete floors and walls and the new lab is in a carpeted room with plasterboard walls. I've read about plenty of members succefully cultivating in places that are not super clean. 

I've read that if I make all my transfers in front of the flow w good technique, where my cultures colonise won't be jeopardised.

I think I'll have to address a few things all at once to get to the bottom of it.

I always appreciate the responses from this community and the ideas pull offer. Theres nothing more frustrating than pulling my hair out and having no one to trouble shoot the problems with. Can always rely on this forum.
:leocheers:


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: Markamello]
    #27608805 - 01/06/22 08:35 AM (2 years, 23 days ago)

I, too, got hit with induction into the Green Hell Club. I went along for about 6 months with decent, not perfect, results. Then one shoebox went green and since then it has been contam city. :facepalm3:

I am taking delivery of a Silver Bullet fogger and Conobrium or whatever the fuck the mold remediation chemical is called. Fogging the whole house except the lab. Then fogging the lab probably twice. I also have a green laser coming to check my FF'U/work area for particulate prevention efficacy. Whoa. I should probably change the pre-filter anyway, soon.

I would post workflow videos...Smart suggested it and I already have some that I was shooting, BUT does anybody now how to post videos without having them open PID in Vimeo or YouTube? I can't be linking my cult stuff to my professional video account.

At least the Reishi don't give a fuck and seem to eat contams for breakfast. I am loving the Reishi grows for æsthetics and for emotional healing after my actives fuck up.:biggrin:


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleQM33
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27608809 - 01/06/22 08:41 AM (2 years, 23 days ago)



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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: QM33]
    #27608820 - 01/06/22 08:53 AM (2 years, 23 days ago)

I got my eye on this well-reviewed one.

Swift 380T


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



Edited by SpaceBaby (01/06/22 08:54 AM)


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Offlinerockyfungus
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Re: Cultivation General Discussion [Re: Markamello]
    #27608859 - 01/06/22 09:25 AM (2 years, 23 days ago)

Quote:

Markamello said:
Zeems I could be forgetting cult 101, contams comes from dodgy spawn.

The warmer weather could be accentuating a flaw in my technique.





If you're working with anything actively sporulating. Shut hood off and work like 30seconds to a minute later.


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InvisibleMysticMycologist
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27608909 - 01/06/22 09:59 AM (2 years, 23 days ago)

Is a myco-quart about 3/4 a quart of colonized grain, or is 2 quart jars of colonized grain equal 1 1/2 myco-quarts?


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Two eyes to look, One eye to see.
Prying open my third eye


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: MysticMycologist] * 1
    #27608915 - 01/06/22 10:03 AM (2 years, 23 days ago)

Myco quart usually means about 3/4 full.


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LAGM2020


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OfflineSalty Dog
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27609615 - 01/06/22 08:06 PM (2 years, 23 days ago)

What's happening in this pic?  Darker stringy elements in there now.



Edited by Salty Dog (01/06/22 08:22 PM)


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Salty Dog]
    #27609650 - 01/06/22 08:27 PM (2 years, 23 days ago)

Quote:

Salty Dog said:
What's happening in this pic?  Darker stringy elements in there now.






Looks like strands of coconut.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: tedoro]
    #27609703 - 01/06/22 09:02 PM (2 years, 23 days ago)

Looks normal, just rhizo knotting up.


The surface is getting matted from being too wet and drying out though. Try to dial the tub in so the conditions don’t swing as far either way.


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OfflineSalty Dog
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Re: Cultivation General Discussion [Re: A.k.a]
    #27609715 - 01/06/22 09:18 PM (2 years, 23 days ago)

AKA - where do you suggest I start?  I've been misting and fanning twice daily.  Lay off the misting?  Appreciate the advice.


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Invisiblebigfootscreepyuncl
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Re: Cultivation General Discussion [Re: Salty Dog]
    #27609721 - 01/06/22 09:22 PM (2 years, 23 days ago)

Quit fanning all together. That's not how FAE is provided and all it does is dry out your substrate


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InvisibleBeano
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Re: Cultivation General Discussion [Re: bigfootscreepyuncl]
    #27609735 - 01/06/22 09:35 PM (2 years, 23 days ago)

@Salty Dog
Someone explained fae recently which made sense to me.
In your case, imagine if you were limited to breathing twice daily. Y'know?


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I can do things you cannot, you can do things I cannot; together we can do great things.


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OfflineRegis
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Re: Cultivation General Discussion [Re: Beano]
    #27610006 - 01/07/22 05:14 AM (2 years, 22 days ago)

For all the yuropoors, look at this shit
28 quart jars and 40 PSI are nothing for this monster:
https://autoklav.at/product_info.php?info=p243_autoklaven--edelstahl-age40--fuer-40-glaeser-0-5-liter-oder-28-glaeser-1-liter.html



And only 650€, thats a steal


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OfflineTweeq
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Re: Cultivation General Discussion [Re: Regis]
    #27610046 - 01/07/22 06:46 AM (2 years, 22 days ago)

Quote:

Regis said:
For all the yuropoors, look at this shit
28 quart jars and 40 PSI are nothing for this monster:
https://autoklav.at/product_info.php?info=p243_autoklaven--edelstahl-age40--fuer-40-glaeser-0-5-liter-oder-28-glaeser-1-liter.html



And only 650€, thats a steal




I must say that is a great deal


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Inocuole]
    #27610187 - 01/07/22 09:13 AM (2 years, 22 days ago)

Is there ever a thought given to how long grains are colonized before they are put into fruiting? And how the resulting flushes might vary?

I decided to not shake a master jar... just to watch the pretty clean growth. It about doubled the time to 100% compared to the thrice shaken jars.

Well some of those earliest grains got a lot longer colonization time than their shaken counterparts.

Could that result in a faster, more robust flush?


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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InvisibleScrimshaw
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Re: Cultivation General Discussion [Re: tedoro]
    #27610212 - 01/07/22 09:43 AM (2 years, 22 days ago)

The debate has always been do jars that have been shaken multiple times lose energy when it comes time to fruit.

I don’t think anyone would disagree that jars that are shaken colonize faster.

Personally, I would rather have jars finish quicker and get mixed with bulk sooner. I don’t think any/many consolidate grain jars after full colonization. Even if yield was increased slightly, that extra time comes at a cost too. Those grains still need to colonize the sub and fruit.

For every week of consolidating your grain spawn you’re 1 week behind having a fully colonized tub that would start to pin within the following week. I don’t think it’s ever been demonstrated that longer colonization/consolidation leads to better yield. Waiting longer after full colonization to spawn just means longer to wait.

I do A2G so I normally shake a week in, and then again a week or two later finishing up in about 3 weeks total. I’ve never thought to myself that the second extra shake was hurting my flush. If anything, I think it’s a detriment to have uncolonized grain in the jar for longer vs getting them colonized quickly.


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Scrimshaw]
    #27610221 - 01/07/22 09:53 AM (2 years, 22 days ago)

I agree with most of that. But... My workflow is only controlled by my fruiting tubs. If my spawn takes longer to make... but shortens my fruiting tubs, its only making things faster. I have all the time in the world to make spawn. Its all about my tubs time.

I'm not too concerned about any harm from the shake... its the lightly colonized grains being fruited... compared to a deeply, completely colonized grain that I am pondering on.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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