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milkboy
Child



Registered: 05/01/21
Posts: 2,299
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Re: Cultivation General Discussion [Re: Mr Piggy]
#27604048 - 01/02/22 03:30 PM (2 years, 27 days ago) |
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Really like these, 20$ but they are really thick. Been using the same pair for a few weeks.Amazon
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Mr Piggy] 1
#27604146 - 01/02/22 04:42 PM (2 years, 27 days ago) |
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Quote:
Mr Piggy said:
Quote:
bigfootscreepyuncl said: What is everyone paying for gloves right now? I just got back from bigbox store and they were trying to charge $23 for a box of the shitty, low quality nitrile gloves! I think I have like 3 individual gloves left right now so I wont be getting much work done until I figure that out
19.99 for good nitrile, but honestly I just wash my hands really well and rub down with alcohol before working 
I did some testing with agar plate contams in a SAB last fall (still writing it up) and found that scrubbed bare hands (30 seconds with soap and water like the CDC says) then rinsed with ISO didn't contam at all when rubbing agar plates with fingers (last pic after 6 weeks):
OTOH ISO'd nitrile gloves out of the box showed lots of bacterial contams after touching the plates directly (2.5 and 6 weeks):

Moral is of course that ISO alone doesn't kill bacteria spores, but soap and water, and or flame, does. As does bleach but it has to be in contact long enough to penetrate the spores. And maybe that flaming hands is not a good idea though I've set ISOd gloves on fire once or twice. 
I still use the gloves because the alcohol really kills my hands after a while, if repeated at all. And working with more than a few cultures involves lots of rinsing inbetween steps at least the way I've generally done it. 
I need to test nitrile gloves that have been scrubbed and then ISO rinsed for the next go round. That and indirect transfer (think rubbing fingers together over a plate) rather than touching the actual agar plate to see what falls off. The next time I do some cult work (that'll be today I'll try that last test after doing a bunch of work with the same gloves that were only ISO'd, maybe do a scrub/ISO as well if I remember and can afford to waste a pair (the price shot up after the pandemic).
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 9 hours
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27604273 - 01/02/22 06:15 PM (2 years, 27 days ago) |
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Anyone ever shipped dried out DRy grains internationally?
I guess Enigma could be more readily transferred like that, opsec wise, than a dish?
Get like 100g of dried grainspawn, revive?
From what I've read it's a 50/50 chsncey that it revives
Dunno why mislabeled Petri dishes cannot be sent internationally Why
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (01/02/22 06:19 PM)
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SpaceBaby
alchemist



Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
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I'll tell you guys, about 3 months ago I geot hit with trich once, and have been loosing 90-95% of smy shoeboxed since.
Sucks.
-------------------- SpaceBaby SPACEBABY'S LAGM22 THREAD MUSHBOY'S SHROOM TEA TEK Me as a cube
Another Day's Work:
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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Re: Cultivation General Discussion [Re: SpaceBaby]
#27604295 - 01/02/22 06:31 PM (2 years, 27 days ago) |
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That really sucks man. I work in the basement of a moldy old house build in the 50s. I do get some contams, but I never really thought spore load would be an issue. Guys it depends on what spores.
-------------------- Two eyes to look, One eye to see. Prying open my third eye 
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 4 hours, 22 minutes
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Re: Cultivation General Discussion [Re: SpaceBaby] 1
#27604306 - 01/02/22 06:35 PM (2 years, 27 days ago) |
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Does anybody else randomly get logged out??
Seems like a couple times a year I hit the bookmark for my threads page and have to log in. Haven’t been able to detect a pattern yet though 
Also sporeload is only a problem if you’ve got holes in your game. IME if you don’t clean your area you’re definitely more and more likely to get mold as time goes on. Once you figure out where you’re making mistakes and fix them it won’t matter anymore.
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LAGM2020     
Edited by A.k.a (01/02/22 06:40 PM)
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SpaceBaby
alchemist



Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
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Re: Cultivation General Discussion [Re: A.k.a]
#27604309 - 01/02/22 06:36 PM (2 years, 27 days ago) |
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i use a imac pro, macbook pro, ipad pro and iphone 13 pro max.
when I log into to a different one than I was in I get booted from the rest.
-------------------- SpaceBaby SPACEBABY'S LAGM22 THREAD MUSHBOY'S SHROOM TEA TEK Me as a cube
Another Day's Work:
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: Cultivation General Discussion [Re: A.k.a]
#27604327 - 01/02/22 06:48 PM (2 years, 27 days ago) |
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Quote:
A.k.a said: Does anybody else randomly get logged out??
Seems like a couple times a year I hit the bookmark for my threads page and have to log in. Haven’t been able to detect a pattern yet though 
Also sporeload is only a problem if you’ve got holes in your game. IME if you don’t clean your area you’re definitely more and more likely to get mold as time goes on. Once you figure out where you’re making mistakes and fix them it won’t matter anymore.
I've noticed I get logged out if I click on a shroomery link from a google search while logged in at the same time
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I 5318008 NOT a virgin!
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CocaineBuffet
Stranger



Registered: 08/29/19
Posts: 3,459
Last seen: 1 hour, 30 minutes
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Quote:
smalltalk_canceled said:
Dunno why mislabeled Petri dishes cannot be sent internationally Why
Do you have evidence that supports this? I am in the US so I don't know how things work in EU
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Lemgrub



Registered: 04/02/18
Posts: 589
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Re: Cultivation General Discussion [Re: A.k.a]
#27604426 - 01/02/22 08:19 PM (2 years, 27 days ago) |
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Quote:
A.k.a said: Does anybody else randomly get logged out??
All the time. I was logged into a strange account once too.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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Doctor Mario
🍄🍄🍄🍄🍄



Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
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Re: Cultivation General Discussion [Re: Lemgrub]
#27604476 - 01/02/22 09:11 PM (2 years, 27 days ago) |
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I found my way into that strange account once too. Tripped me out. It was like a ghost account that didn't actually exist.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Quote:
CocaineBuffet said:
Quote:
smalltalk_canceled said:
Dunno why mislabeled Petri dishes cannot be sent internationally Why
Do you have evidence that supports this? I am in the US so I don't know how things work in EU
put a wedge in a dimebag. it will get smooshed in the mail but will grow great once transferred. thanks haywire!
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
Edited by TedsDead (01/03/22 12:33 AM)
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WyoMX

Registered: 07/06/15
Posts: 2,102
Loc: PNW
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Re: Cultivation General Discussion [Re: TedsDead]
#27604694 - 01/03/22 05:17 AM (2 years, 26 days ago) |
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Quote:
Ythan said: Cool, thanks, I always use "remember me" so that could explain why these issues flew under my radar for so long. 
One other thing to note if you don't use "remember me" is that sessions have a 24 hour lifetime. It's automatically extended every time you view a page, but if you just leave the site open in the background and don't interact with it, after a day you will be logged out. That's expected behavior.
Quote:
Ythan said: Okay, I found and fixed another login related problem. If you were signed in without the "remember me" option, and then you logged in on another device or browser, you'd get logged out of the first one. Or, if you didn't use the "remember me" option and then logged out, it would log out every device where you hadn't selected "remember me". This could have been responsible for some of what people were experiencing.
I don't have an explanation for why you'd get randomly signed out after navigating to a different page, unless maybe your sign-in cookie had just expired after 120 days, or you changed your password on a different device. I will keep investigating. I'd also be interested to know if either of the fixes I made seem to have helped. Could you please keep an eye out, and when/if it happens again, let me know if you were browsing with "remember me" selected?
I believe out lord and savior is checking into things about getting logged out randomly. It use to happen to me more but not so much anymore. Also I nocced up some jars today with PE, melmac and then some agar of what's supposed to be leucistic melmac, leucistic treasure coast, and a different set of PE genetics. I've got more plates to make soon, as I wanna put some wg and apeu on agar asap as well.
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rockyfungus
dirty



Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 10 hours
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Re: Cultivation General Discussion [Re: TedsDead]
#27604722 - 01/03/22 06:08 AM (2 years, 26 days ago) |
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Double thanking Haywire. Little dime bag with agar turned to liquid. They said it's fine to go right from liquid agar. Me liking agar better then LC threw it in the fridge and was clean on T1
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 4 hours, 22 minutes
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Re: Cultivation General Discussion [Re: rockyfungus] 1
#27604801 - 01/03/22 08:20 AM (2 years, 26 days ago) |
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I’ve put wedges into empty 00 capsules a couple times, worked out ok.
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LAGM2020     
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: A.k.a]
#27604846 - 01/03/22 09:13 AM (2 years, 26 days ago) |
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Ive mailed whole petris plenty of times.
I typically take a piece of styrofoam, cut it into a rectangle that fits snug inside the smallest priority mail box which typically cost around 8 bucks to ship, then cut a circle the size of a petri out of the center of the styrofoam rectangle.
Fit the petri into the hole then slide the styrofoam rectangle into the priority box.
I've mailed multiple petris this way with no issues.
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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 8 hours, 28 minutes
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Re: Cultivation General Discussion [Re: A.k.a]
#27604848 - 01/03/22 09:14 AM (2 years, 26 days ago) |
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Hmm, so I was asking a few weeks ago if it would be ok to transfer mycelium from a fully grown dish I've had sitting since June so 7 months now.
I made the transfer, seems to be taking off completely fine, good to know. Now I have two new varieties started 1/1 from spore and a few plates with B+ mycelium already goin
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: Cultivation General Discussion [Re: PreparationH]
#27604968 - 01/03/22 10:57 AM (2 years, 26 days ago) |
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For anyone that sterilizes agar and melts it at another time, how do you re-melt it?
I need to sterilize my scalpels and loops today to do some inoculations and transfers as well as make some agar to pour some new plates but I won't be able to pour before the bottles solidify and I would prefer to not run my PC twice. Backing the lid off the media bottle to microwave makes me nervous because it sucks air in and I have cracked media bottles in the microwave by not backing the lid off. I always just pour before the agar solidifies but I did a poor job of planning my workflow so far this year...stupid LAGM lol
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I 5318008 NOT a virgin!
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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I microwave with the lid closed. Short bursts with swirling until it’s melted. If you get it around the temp that you sealed it, there is not much vacuum or pressure and it opens easily. You could probably use a simmering water bath as well if the microwave overheated and cracks the bottle.
-------------------- Two eyes to look, One eye to see. Prying open my third eye 
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
Last seen: 9 hours, 10 minutes
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I lossen the cap, microwave in bursts Comes out 160ish, haven't had an issue.
I keep sterilized media bottles in the fridge with parafilm around the cap.
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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