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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Josex]
#24146418 - 03/08/17 01:53 PM (6 years, 10 months ago) |
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k I will add a bit more.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
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Quote:
TheMadHatter420 said: I plan on sacrificing on of my PP5 pasty plates to inoculate a LC. going to take an LC needle full of water and sterile, heat tip glowing red, stick through plastic, shoot water onto culture, scrape around gently with needle and suck it all back up, thus creating what should be a sterile LI. I will pull out, heat up, stick tip in jar, let cool a bit then shoot all that LI into the LC. Should leave little vector for contamination to get in. I got a BEAUTIFUL and clean pasty plate for this 
IMO, the chances of contaminating the lc are going to be high if you do that. Chances are there is some hidden bacteria in the agar plate and also the whole process you describe sounds very risky to inoculate a LC.
Check my journal, that's how I inoculate lc's and it is really tough to fuck up if you do that.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Josex]
#24146468 - 03/08/17 02:13 PM (6 years, 10 months ago) |
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I actually found your journal before. I did that with the LC I am about to test, although I stabbed a few times and got multiple "plugs" in the needle for multiple inoculation points.
I have been hunting for an agar plat that looks this nice. It is a clone transfer several times. I know LC can contaminate easily. People use whole plates for Master jars.
I figure a fully glowing red needle, should go right through the lid easily, while not making a huge hole. I don't even have to shoot that much water out, or I could even do your plug method. I figure at about $0.25 each, these PP5's are disposable and I will go right through it with a hot needle, into a sterile environment inside.
Hmmmmm... In future I could do one final transfer to antibiotic agar before I take some to knock up my LC. Wouldn't have to worry about hidden bacteria.
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Josex
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I don't know how you got multiple plugs in the needle I stab just once and the needle always looks totally clean, no visible signs of myc or agar whatsoever. But yeah stabbing more times works as well, but it's a little bit riskier and also the point of stabbing a single spot is to narrow down the genetics an awful lot in one single transfer (can be useful if you are working with ms).
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: Josex]
#24146685 - 03/08/17 03:45 PM (6 years, 10 months ago) |
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Dude, pastys EZLC is back in action! Why not make a potatoe flake, dog food, BRF, and bran liquid culture instead of just a measly run of the mill LME LC? Your myc will thank you later
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Josex
#cheat_code


Registered: 11/13/15
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Re: Cultivation General Discussion [Re: ComebackKid]
#24146704 - 03/08/17 03:52 PM (6 years, 10 months ago) |
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I love pasty's teks but to be honest, I never liked that one, ships, several steps, adding water... to each their own though. Have you had trouble with run of the mill lme lc's btw? Because lme is widely use to make agar by not so run of the mill cultivators.
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: Josex]
#24146786 - 03/08/17 04:21 PM (6 years, 10 months ago) |
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I personally can't get a culture that prefers my lme agar over say brfa. It puffs and gets ugly on me every time.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: Josex]
#24146789 - 03/08/17 04:22 PM (6 years, 10 months ago) |
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No I don't even have LME But I have made LC before with success. I was just hyping up the fact that you could use anything in an LC if you really wanted to. I know LME is better than honey at least.
Have you tried the EZ LC though? I dont understand how anyone could not be a fan. You're gunna have some sort filter on a LC jar, you don't nessisarily need a whatman and SHIPs to do it. Just use whatever you're gunna use on your regular LC
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Josex
#cheat_code


Registered: 11/13/15
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Re: Cultivation General Discussion [Re: ComebackKid]
#24146825 - 03/08/17 04:40 PM (6 years, 10 months ago) |
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I know the idea is very solid, the problem is that I personally really dislike ships. A 14G needle won't have problems to aspirate a relatively thick lc to a certain extent but we know that that gauge will destroy the ship. Also, everytime you use a ship you're adding vectors. Regarding the super lc's where you just pour the water, well I'm not comfortable with that I understand Pasty has a lot of experience in the SAB but it's not my case.
For me lc's are one use only, I pour them, therefore I don't test them, that's why I have to make sure that the way I inoculate them is very safe.
Anyway, that's the beauty of mush cult, you try things tested and untested until you find something that really suits your style, or you can follow what others with more experience do too, no fuss no muss.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Wicked Burn]
#24146832 - 03/08/17 04:43 PM (6 years, 10 months ago) |
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Quote:
Wicked Burn said: Anyone know if/what the limit is for a basic water extract? How many dry grams worth of active chemicals you could put into say 400 ml of water? I'm thinking I want to take all my stash and make a water extract out of it and freeze it in ice cube trays. Would like to make it as concentrated as possible to take up as little space in my freezer as possible.
No but I've put like a half into a pint of water before,
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
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Re: Cultivation General Discussion [Re: Josex]
#24146847 - 03/08/17 04:49 PM (6 years, 10 months ago) |
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Trying to get LC into a syringe through an 18g needle is damn near impossible I hear ya. 16g works well for me unless it's really thick. I should consider 14 though.
I have never used a SHIP other than the first time I tried nocking up a jar of grains with 10cc spore solution And probably will never use one again because of mush cult
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
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Re: Cultivation General Discussion [Re: ComebackKid]
#24146873 - 03/08/17 05:00 PM (6 years, 10 months ago) |
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I use lme for LC and have a double silicone port lid configuration. Put 14g needle through one of the silicone ports and slide a whatman filter onto it. PC like that and extract with ease. Can set on its side and can pull LC with ease. I recommend this method, basically the only way I've had ease with LC.
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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Josex
#cheat_code


Registered: 11/13/15
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Re: Cultivation General Discussion [Re: Lobi]
#24146898 - 03/08/17 05:10 PM (6 years, 10 months ago) |
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Sounds good! Tbh I'd rather use a syringe instead of pouring the lc, but the ship is a big no no for me.
I ordered an autoclavable, cheap af automated syringe gun from ebay, the ones used for cattle, I'm still waiting for it.
What I plan to do is silicone a 15 cm 4G stainless steel needle to the lid that reaches the bottom of the jar and connect the gun to the needle, as an inoculation port I have a cool idea lurking in my mind too, we'll see. I can't wait to get this shit done 
http://m.ebay.es/itm/401255219776
Edited by Josex (03/08/17 05:18 PM)
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: Cultivation General Discussion [Re: Lobi]
#24146910 - 03/08/17 05:12 PM (6 years, 10 months ago) |
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On another subject do you guys have any insight on sub depth? I am thinking if the substrate is loose enough should it matter? Please check this out and let me know what you think of This grow any insight is welcome
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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Wicked Burn


Registered: 05/15/16
Posts: 254
Last seen: 7 days, 9 hours
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Re: Cultivation General Discussion [Re: bodhisatta]
#24147425 - 03/08/17 08:50 PM (6 years, 10 months ago) |
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Quote:
bodhisatta said:
Quote:
Wicked Burn said: Anyone know if/what the limit is for a basic water extract? How many dry grams worth of active chemicals you could put into say 400 ml of water? I'm thinking I want to take all my stash and make a water extract out of it and freeze it in ice cube trays. Would like to make it as concentrated as possible to take up as little space in my freezer as possible.
No but I've put like a half into a pint of water before,
Nice, I think I can work with that. Thanx man.
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Mr. Pink: "Hey, why am I Mr. Pink?" Joe: "Because you're a faggot." -Reservoir Dogs
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Wicked Burn]
#24147797 - 03/09/17 12:18 AM (6 years, 10 months ago) |
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What up.
Just cooked 10 pasty plates in my new PC. That was a breeze. The racks... so convenient! I put one in the bottom, then a shot glass in the middle, then the next rack on top. Balanced my pasty plates and cooked away. The water was never directly submerging the bottoms of the plates as far as I could tell. seemed to go well.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Moabfighter]
#24147907 - 03/09/17 02:04 AM (6 years, 10 months ago) |
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So I just found 3pk eco earth coir bricks for sale online for 4.99 each. Site offers free shipping on any order over $49. Considering the price of shipping ~30 bricks of coir, I might have to jump on this. Would you?
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Kenetic
Nam Sayin



Registered: 08/24/14
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I just paid 30 bux for three 3 packs at the pet store. Your price is half of that plus shipping. You decide lol
Quote:
Lobi said: On another subject do you guys have any insight on sub depth? I am thinking if the substrate is loose enough should it matter? Please check this out and let me know what you think of This grow any insight is welcome
I stick around 2-3 inches
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Crispykoot
Jello Wrangler



Registered: 10/16/16
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Re: Cultivation General Discussion [Re: Kenetic]
#24148090 - 03/09/17 05:54 AM (6 years, 10 months ago) |
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Excalibur question....How many trays do you actually get into the 9 tray version at once? I might get 5 in there if the shrooms arent too big..
These things feel very over priced...I want to build one, but...no time right now...
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Shadowboxing the apocalypse and wandering the land
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: Crispykoot]
#24148111 - 03/09/17 06:20 AM (6 years, 10 months ago) |
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I have the same issue with my excalibur mate.
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