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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: Doctor Mario]
#27524722 - 10/30/21 10:35 PM (2 years, 2 months ago) |
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yeah, I got one food coloring I couldnt use because it killed the myc. I never bothered to look at what was in it tho. some hippy "natural" one
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 8 hours, 42 minutes
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Re: Cultivation General Discussion [Re: TedsDead]
#27524751 - 10/30/21 10:58 PM (2 years, 2 months ago) |
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I've had a lot of trouble somewhat recently with all of that shit I believe. Each and every one of those examples looks sorely familiar
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 8 hours
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27524807 - 10/31/21 12:05 AM (2 years, 2 months ago) |
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Sick P9. I use antibiotic interchangeably. If you want to be pedantic (?) Biotic=life. Clinically I'd get my ass fucking chewed out. Fuck those old doctors though...
I need to dig a bit. What other pathogenic processes destroy the fungi? I'd imagine some target steroids or ergosterol?
I guess chitnaneses make most sense for scalloped margins. "mycogone" happens more intracellularly from what I've seen macroscopically. Really need to start playing microscopically as I'm used to using a scope for hours a day looking at a biological structure that my mind keeps trying to tie together with similar pathology in fungi...
Anti-fungals are some serious heavy duty fucking shit when used systemically. Triazoles 
Edit: fucking Halloween theme as I hit submit, trippy scared me!
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 8 hours, 42 minutes
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Re: Cultivation General Discussion [Re: rockyfungus]
#27524813 - 10/31/21 12:23 AM (2 years, 2 months ago) |
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Orange and ghosts!
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Drboomer
The lord magnificent


Registered: 09/22/19
Posts: 957
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Re: Cultivation General Discussion [Re: Failboat]
#27524825 - 10/31/21 12:39 AM (2 years, 2 months ago) |
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Screwed me up for a few seconds lol
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psh
Stranger
Registered: 05/06/21
Posts: 177
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Re: Cultivation General Discussion *DELETED* [Re: Drboomer]
#27524883 - 10/31/21 03:46 AM (2 years, 2 months ago) |
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Post deleted by psh
Reason for deletion: .
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: psh]
#27524885 - 10/31/21 03:50 AM (2 years, 2 months ago) |
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its better just to not use plates like that. with ms its best to make alot of plates from the start and keep only the best ones. with each step youll have less and less to worry about as u continue to choose the best looking plates to work with
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: rockyfungus]
#27524912 - 10/31/21 05:01 AM (2 years, 2 months ago) |
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Quote:
rockyfungus said: I guess chitnaneses make most sense for scalloped margins. "mycogone" happens more intracellularly from what I've seen macroscopically.
Considering that mycogone and Trichoderma both produce Chitinases it seems reasonable to suspect that these pathogens are not only growing within our plate cultures but affecting their morphology by preventing growth of the host organism where these products are produced.
flocculent mycelia with strange inhibitory zones:

Photo: multifractal
I was stumped by the fact that observation under a scope didn't reveal much, but the links on the previous page seem to have cleared that up for me. The white, fluffy, less virulent colonies of mycogone lacked any real visually identifiable marks like conidia/conidiospore.
Chitinases or some similar mechanism seems reasonable.
Edited by Stipe-n Cap (10/31/21 05:15 AM)
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 1
#27524923 - 10/31/21 05:23 AM (2 years, 2 months ago) |
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Man this is the best page of general I ever done seent.
more pics of bad guys. This mycogone culture matches one of the types on the other pic pretty good.
I'm still pretty pissed that I have to learn how to spell lecana-whatever-the-fuck. Do you know how long it took me to learn verticillium? Huh? DO YOU!?
also got this pic recently from a melmak swab that i guess i stuck up my butt.



Edited by sandman420 (10/31/21 06:09 AM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: sandman420]
#27524949 - 10/31/21 06:21 AM (2 years, 2 months ago) |
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Nice pics, I regret having deleted all of my cult photos.
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27524961 - 10/31/21 06:34 AM (2 years, 2 months ago) |
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Good read on chitinase. Gets into bacteria and fungal chitinase about a third in. Still reading . https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612335/
So a plate could be infected with mycogone P., exibit signs of chitinases (scalloping?), And or even extrcete metabolites as well as unusuall growth patterns.?
Good shit
**
""It is considered that in order to supply nitrogen and carbon as a source of nutrients, bacteria mainly produce chitinases.[44] The production of chitinases in bacteria is mainly for the degradation of chitin and its utilization as an energy sourc
Edited by QM33 (10/31/21 06:37 AM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: QM33]
#27524962 - 10/31/21 06:36 AM (2 years, 2 months ago) |
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That seems like a reasonable estimate to me. Symptoms would reflect stage of infection, virulence etc.
I would wager that the method of spore collection may potentially have some correlation to infection, as we see this often with varieties that are difficult to print: Melmak and APE, for instance.; though admittedly this is far more of a stretch.
Edited by Stipe-n Cap (10/31/21 06:51 AM)
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 2
#27524965 - 10/31/21 06:43 AM (2 years, 2 months ago) |
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I love when some good info gets going!
Happy Halloween btw mofks! Hope everyone can take a minute to enjoy and appreciate themselves.
#chitinasesbeotch #halloweenmofky ##
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Nimpo
Big Black


Registered: 05/10/12
Posts: 2,375
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Re: Cultivation General Discussion [Re: QM33]
#27525113 - 10/31/21 09:16 AM (2 years, 2 months ago) |
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Coir shortage finally hit my city. No stores in a 12 miles vicinity have it in stock, pretty wild
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Nimpo]
#27525133 - 10/31/21 09:30 AM (2 years, 2 months ago) |
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Get straw pellets.
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27525136 - 10/31/21 09:32 AM (2 years, 2 months ago) |
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Quote:
p9hu7 said: Get straw pellets.
Wait a minute... That actually sounds like a good idea beyond the shortage no? Probably way cheaper.. how would water capacity compare you think? And I'm seriously sick of the coir I get from the farm store, full of rocks and sticks, a pain to break up and mix. But I mean what would be the downside of strawpellets.?
Okay I need to see a straw pellet grow now!
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: QM33]
#27525142 - 10/31/21 09:37 AM (2 years, 2 months ago) |
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So I mixed up a sub just to see what the consistency would be like, I didn't inoculate it though.
I used:

Recipe mixed and sat in a sealed 3t Unicorn bag for a month to see if it would Tam.
10 cups of straw pellets 1 liter vermiculite 1250ml hot water
May want to tweak that Recipe to produce a larger substrate but that would make a decent substrate bag.
I was fixing to make a "straw boss" tek that was intended to be a coir alternative Recipe before I packed up my lab, this is as far as I got.
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27525151 - 10/31/21 09:42 AM (2 years, 2 months ago) |
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Well please let me know what happens! Did you get it to a field capacity or? I wish you did it by weight honestly! Shiet I need to go grab a bag! I seriously hate my brand of coir more and more everyday, and I don't want to pay anymore than I do at like 5$ for 5kilos. But I can get straw pellets for like 7$ a 40lb bag.
Ide like to see how 250g of each holds a liter.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: QM33]
#27525153 - 10/31/21 09:44 AM (2 years, 2 months ago) |
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I'm all packed up so I won't be developing further atm, that mix was field capacity, the consistency looked great and I have no doubt that it would work well.
The pellets are cleaned of all dust and debris so it lends itself to hot water hydration much like coir, verm is definitely required.
It's a very cheap, clean alternative to coir. It should work but people need to run it live to see how it performs as a substitute.
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27525159 - 10/31/21 09:47 AM (2 years, 2 months ago) |
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Why is verm required thooooooooo. Why not just more sub? I hate an extra thing to buy, that cost so much relatively.
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