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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Nimpo]
    #27523845 - 10/30/21 09:14 AM (2 years, 2 months ago)

It happens, I either add micropore until it evens out or just give it a mist.

My top layers are soo thick though that having a thin layer of surface dry out isn't much of an issue.


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InvisibleNimpo
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27523869 - 10/30/21 09:47 AM (2 years, 2 months ago)

Quote:

p9hu7 said:
It happens, I either add micropore until it evens out or just give it a mist.

My top layers are soo thick though that having a thin layer of surface dry out isn't much of an issue.




Excellent, good to know :cool:

Been lightly misting the 4 small areas daily, but Im gonna try to dial it in better with more tape. Lots of knots forming on these PE tubs, will drop some pics in your pseudocasing thread once the flush comes in

:raisemyglass:


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Nimpo]
    #27523889 - 10/30/21 10:01 AM (2 years, 2 months ago)

Could even just block off the bottom holes altogether until the pins come in, then open them up as the flush matures.


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OfflineSawakaze
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Re: Cultivation General Discussion [Re: ModularMind]
    #27523908 - 10/30/21 10:18 AM (2 years, 2 months ago)

It's a north spore "Golden Oyster Mushroom Grow Kit." It's one of the blocks of mycelium that's apparently ready to go with some scraping & water. I'm looking at picking up some syringes from ralphster spores too.


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Offlinerockyfungus
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Re: Cultivation General Discussion [Re: JXAllen]
    #27524009 - 10/30/21 11:58 AM (2 years, 2 months ago)

Quote:

JXAllen said:
Quote:

rockyfungus said:
For glass petris, any benefit of oven instead of PC?



If you PC them in aluminum foil, they will come out filled with condensation. In the oven, they obviously stay dry. If anyone knows how to PC them without them filling with moisture, please chime in.




Guess I'm just so used to the condensation at this point. (Also individually wrapping top and bottom and really getting a good seal prevents condensation. Rare but happens if I wrap correct.

Fuck that's why my last dish contamed thank you! I tried drying it with my lysol rag to see if that works. Totally forgot that part. Instead of just using my flow like normal. (or upside down in a SAB to dry)


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Invisiblepsh
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Re: Cultivation General Discussion *DELETED* [Re: Sawakaze]
    #27524010 - 10/30/21 12:00 PM (2 years, 2 months ago)

Post deleted by psh

Reason for deletion: .


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InvisibleShroomerInTheRye
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Re: Cultivation General Discussion [Re: psh]
    #27524042 - 10/30/21 12:29 PM (2 years, 2 months ago)

Those bubbles look like the start of mycogone.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: ShroomerInTheRye]
    #27524049 - 10/30/21 12:34 PM (2 years, 2 months ago)

I'm not sure what it is, whatever it's designation it always spells trouble for me. I toss my tubs when I see it, only ever on tubs and almost always associated with scalloped plates, though I know that's a bit of a contentious subject.


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InvisibleQM33
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Re: Cultivation General Discussion [Re: psh]
    #27524058 - 10/30/21 12:42 PM (2 years, 2 months ago)

Quote:

psh said:
Found this on an old plate. Mycogone or something else?




Idk about mycogone, definitely bacterial.Looks less bubbly and more like metabolites, but the way the myc is growing is more concerning then those droplets, to me. Wet bubble appears as flesh.


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InvisibleJohnnieYen
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Re: Cultivation General Discussion [Re: Sawakaze] * 1
    #27524096 - 10/30/21 01:10 PM (2 years, 2 months ago)

Quote:

Sawakaze said:
It's a north spore "Golden Oyster Mushroom Grow Kit." It's one of the blocks of mycelium that's apparently ready to go with some scraping & water. I'm looking at picking up some syringes from ralphster spores too.




go with sporeworks


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[center


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InvisibleQM33
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Re: Cultivation General Discussion [Re: JohnnieYen]
    #27524129 - 10/30/21 01:38 PM (2 years, 2 months ago)

Soooooo speaking of mycogone lol.

I was just reading something under the impression mycogone aka wet bubble disease is the same as mycogone perniciosa.

So is wet bubble disease different than mycogone? And does mycogone cause wet bubble?

https://www.semanticscholar.org/paper/Isolation-and-identification-of-Mycogone-causing-in-Kouser-Shah/2c5b2af21be4a7c1f98ea282998646638af280db#paper-header


And maybe why bod couldn't identify any bacteria in the samples he put on the scope?

And at some point in bids thread someone said it could be contagious.. could wetbubble infect a fruit beyond the means of bacteria..?


And you know I'm reading this now.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963780/#!po=12.2642

And I think I've asked before how you could have mycogone aka wet bubble on a petri. Well ya you could have mycogone p on a plate. But can you have wet bubble disease on a plate that doesn't have at least a pin...? I would say no..?

That second article goes into the genetic diversity of like 18 different mycogone samples and it's pretty wile really imo. I need to finish it


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: JXAllen]
    #27524130 - 10/30/21 01:39 PM (2 years, 2 months ago)

Quote:

JXAllen said:
Quote:

rockyfungus said:
For glass petris, any benefit of oven instead of PC?



If you PC them in aluminum foil, they will come out filled with condensation. In the oven, they obviously stay dry. If anyone knows how to PC them without them filling with moisture, please chime in.




I PC them in a couple old cult bags I've used forever for that purpose, always worked fine.  Oven works too but PC is right there when cooking agar. :shrug:


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: sandman420] * 2
    #27524134 - 10/30/21 01:40 PM (2 years, 2 months ago)

Quote:

sandman420 said:
nalgene gets cloudy




Not yet anyway, liking them a lot over glass.  Carolina Biological for the best price. :hereyougo:


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: PrimalSoup]
    #27524171 - 10/30/21 02:07 PM (2 years, 2 months ago)

Yes wet bubble is the name of the disease in mushroom crops that is caused by mycogone perniciosa. Like "green mold" is caused by trichoderma or other actual green sporing molds.

I'm  :lookslucrative: on that link you posted always a pleasure to read about the boogieman



of corse we would never see it in its pure isolated form like that, probably only in its mycoparasitic form growing with the host.

but you can see D/E looks a fuck lot like tomentose mushroom mycelium and also cobweb.. 


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Edited by sandman420 (10/30/21 02:19 PM)


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InvisibleQM33
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Re: Cultivation General Discussion [Re: sandman420]
    #27524427 - 10/30/21 05:12 PM (2 years, 2 months ago)

Idk. Because with the green mold the green is the spores of the mold iirc.. so you do see the problem. but with wet bubble the bacteria might not even be present in the host? Maybe just on certain spots on the fruit surface or in the substrate along with the mycelium..? But also it could be present throughout the fruit body and covering the surface it seems..? Idk.

And ya D is actually pretty fucking scary ha. Ya it is a good link, and those pictures are fire

Ya that article at least demonstrates how complex this bacterias effects are on agricus at least



Like maybe the bacteria isn't even present in the metabolites or droplets, maybe the fruits and mycelium could all be effected from another infected area? Kind of like a non mycoparasite..?


Edited by QM33 (10/30/21 05:36 PM)


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: QM33] * 1
    #27524498 - 10/30/21 06:26 PM (2 years, 2 months ago)

That last link was very interesting. It's notable that many of the infected cultures  did not exhibit any morphological traits and where white, some possesed rings, others fluffy mycelium with exudates.

With all white-colored colonies having villous texture, these white cultures were less virulent and lacked the presence of conidia. So even under the scope we wouldn't likely be able to identify.

The first one mentions inhibitory zones, I'd like to find out more about culture morphology that includes zones of inhibition.

"Chitinases of pathogenic fungi not only play vital roles in spore germination, septum formation, cell division, and morphogenesis, but the enzymes are also important in the host interaction . In addition to degradation of the host fungal cell wall, chitinases also inhibit hyphae growth and bud tube elongation.

https://www.frontiersin.org/articles/10.3389/fmicb.2020.596719/full


I am convinced that scalloped edges are morphological identifiers for some fungal pathogen, I refuse to believe that inhibitory zones are healthy growth patterns.


These are my only remaining pictures so they'll have to suffice.

flocculent texture with concentric rings and inhibitory zones.



These cultures produced no fruit. The substrate produced large pools of metabolites and small puffballs type structures with beads of metabolites that resembled spiders eyes.


Edited by Stipe-n Cap (10/31/21 05:07 AM)


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Offlinerockyfungus
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27524534 - 10/30/21 07:13 PM (2 years, 2 months ago)

IIRC inhibitory zones are used to test the strength of an "antibiotic".

So it makes sense a scalloped edge is something either destroying the fungi intracellularly or extracellularly.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27524539 - 10/30/21 07:18 PM (2 years, 2 months ago)



Edited by Stipe-n Cap (10/30/21 07:44 PM)


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: Stipe-n Cap] * 1
    #27524687 - 10/30/21 10:09 PM (2 years, 2 months ago)

I 100% agree.  Im not sure what it is but whenever I get plates with that I toss em


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InvisibleDoctor Mario
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Re: Cultivation General Discussion [Re: TedsDead]
    #27524718 - 10/30/21 10:33 PM (2 years, 2 months ago)

I had a few plates that did that when I was using food coloring that had erythrosine in it.



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Edited by Doctor Mario (10/30/21 10:35 PM)


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