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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 10 hours
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27520607 - 10/27/21 07:03 PM (2 years, 3 months ago) |
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Agar work is the most fantastic dice roll wth genetics where you immediately produce new and retain old myc genes/traits/
Blessed work π
Cultures fruiting on agar has changed my ideas about how to go about things and now I have questions
Basic questions about genetics
I think it will be valuable to a lot of growers to correctly understand this part of agar work. Calling all friends.
How correct are these statements:
"Mycelia that will fruit on agar when cloned from correctly will carry genetics that will make it more likely to fruit on anything"
"Tissue cloned from a fruit on a agar plate, carry genes that make the mycelia from that culture able to express the same fruit look, relative size of fruit, how easy/much it pins, under the same temperature conditions."
Applied to both exotic work and to cube growing
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (10/27/21 07:11 PM)
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27520777 - 10/27/21 09:26 PM (2 years, 3 months ago) |
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Mmm, fruiting on agar is a last ditch hail Mary response by the myc I think. But it always seems like an indicator of good fruiting potential in practice.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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SingularFusion


Registered: 10/31/18
Posts: 1,208
Last seen: 3 hours, 40 minutes
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27520784 - 10/27/21 09:33 PM (2 years, 3 months ago) |
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plate tissue clones almost always produce decent subsequent tubs for me
however the actual morphology of the pin that was cloned does not always seem to carry over
I find the same when cloning clusters etc from a tub... doesn't always seem to capture what I was after originally
I too am curious how these things really work with any consistency in practical application, or if it is not just essentially luck of the scalpel stroke on that occasion
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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TedsDead



Registered: 01/03/17
Posts: 4,998
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I agree with primal soup. I also agree that yes its luck of the scalpel stroke. that's why u take lots of clones, test them and pick the ones that have the traits that your after
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27520839 - 10/27/21 10:52 PM (2 years, 3 months ago) |
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Quote:
PrimalSoup said: Mmm, fruiting on agar is a last ditch hail Mary response by the myc I think. But it always seems like an indicator of good fruiting potential in practice. 
Not only an indicator of good fruiting potential, but agar pin clones is the quickest and easiest way to go for fruiting genetics. Most of the time they look like mono cultures when transferred to new plates . I don't even do that much isolation anymore when starting new varieties, just swipe a plate, let it colonise until it's pinning and clone those. super lazy but it works every time
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Oldow
Stranger


Registered: 11/04/14
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27521002 - 10/28/21 02:57 AM (2 years, 3 months ago) |
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Quote:
PrimalSoup said:
Quote:
JohnnieYen said: Can I get your guys opinion on this concept. I got a lot of reddit hate (like a lot) from noobs when I suggested it. https://www.shroomery.org/forums/showflat.php/Number/27506021
TL;DR its starting cultures by using mini grain jars to get myc started, then transferring a colonized grain to agar. The argument is why not just start with a drop to agar, its just an extra step and another chance of contamination. My argument is that i previously used these same syringes to agar and got nothing but bacteria after waiting 3 weeks. dishes pictured in the thread are 15 days from inoculation of grain. 10 days after inoculation the transfer was put to agar and it grew out for 5 days.
I posted this in that thread too:
When you pull colonized grains to agar you'll get much faster growth due to the grain nutes you're bringing along. If you do a transfer to just agar after that they'll slow down considerably.
But I like the syringe => grain => agar route for germination and culture isolation - see https://www.shroomery.org/forums/showflat.php/Number/26369976/fpart/all/vc/1 for a whole PE isolation grow log using this method. 
Worked for me with a dirty syringe. Might have been a fluke though.
I saw mini pf cakes being recommended, the substrate is supposed to be bacteria unfriendly.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: Oldow]
#27521005 - 10/28/21 03:03 AM (2 years, 3 months ago) |
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I dont know why? its wet starchy rice flour
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 5 hours, 45 minutes
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Re: Cultivation General Discussion [Re: TedsDead]
#27521172 - 10/28/21 08:16 AM (2 years, 3 months ago) |
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I saw something about the chunky surface of pf mix traps bacteria. No idea if itβs true but I could see it at least slowing bacteria down.
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LAGM2020     
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Oldow
Stranger


Registered: 11/04/14
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Re: Cultivation General Discussion [Re: A.k.a]
#27521185 - 10/28/21 08:33 AM (2 years, 3 months ago) |
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Obvious advantage is that it is immobile. You will not spread the germs by shaking the grains and the bacteria cannot create those slimy puddles they do on agar. Under these conditions, the mycelium can be able to outrun the infection.
But I dunno, I am guessing here
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Sawakaze
Outlander

Registered: 10/28/21
Posts: 2
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: Oldow]
#27521460 - 10/28/21 12:19 PM (2 years, 3 months ago) |
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Hello, I'm getting a grow kit for Golden Oysters here soon, and was curious if a closet with a shotgun chamber would be ideal? I'm considering adding a computer fan to it to ensure optimal air flow, but for now I just have the basics (tote with holes, some perlite, & a spray bottle.) Is this insufficient for air flow, or other needs I am not aware of? Thank you for your patience, I'm new, I don't know dick even though I've been reading/watching a bit now.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Sawakaze]
#27521589 - 10/28/21 01:50 PM (2 years, 3 months ago) |
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How interesting would it be to work with phages, inoculations done with bactirophage cocktails of various species of phage that target the common species of contaminant bacteria.
Broad spectrum phage inoculation.
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Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 1
#27521704 - 10/28/21 03:36 PM (2 years, 3 months ago) |
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No time to watch this now but this is definitely something Iβm gonna come back to tonight, thanks for sharing
-------------------- Back once again with the ill behaviour Links I like
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Smoothcat]
#27521712 - 10/28/21 03:45 PM (2 years, 3 months ago) |
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This is a good one about phages as well.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 19 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27521736 - 10/28/21 04:03 PM (2 years, 3 months ago) |
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I used some LC, threw 250ml into 3 bags cause I wasn't confident. Shit was good.
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: Cultivation General Discussion [Re: Sawakaze]
#27522229 - 10/28/21 10:44 PM (2 years, 3 months ago) |
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Quote:
Sawakaze said: Hello, I'm getting a grow kit for Golden Oysters here soon, and was curious if a closet with a shotgun chamber would be ideal? I'm considering adding a computer fan to it to ensure optimal air flow, but for now I just have the basics (tote with holes, some perlite, & a spray bottle.) Is this insufficient for air flow, or other needs I am not aware of? Thank you for your patience, I'm new, I don't know dick even though I've been reading/watching a bit now.
Not enough known of the βkitβ.
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Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 1
#27522456 - 10/29/21 05:31 AM (2 years, 2 months ago) |
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Just watched those phage videos, really interesting shit. Actually right up my alley now Iβm taking a new direction in life, nice one man  
-------------------- Back once again with the ill behaviour Links I like
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 5 months, 17 days
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Re: Cultivation General Discussion [Re: Sawakaze] 1
#27522774 - 10/29/21 12:11 PM (2 years, 2 months ago) |
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Quote:
p9hu7 said: This is a good one about phages as well.
Wow, they have a phage for pseudomonas! (Different species than the one that plagues us, though.)
Quote:
Sawakaze said: Hello, I'm getting a grow kit for Golden Oysters here soon, and was curious if a closet with a shotgun chamber would be ideal? I'm considering adding a computer fan to it to ensure optimal air flow, but for now I just have the basics (tote with holes, some perlite, & a spray bottle.) Is this insufficient for air flow, or other needs I am not aware of? Thank you for your patience, I'm new, I don't know dick even though I've been reading/watching a bit now.
Normally oysters grow in bags or buckets with a hole cut where growth will occur. Fruit are sprayed so they can take in water directly, not just from the mycelia. They also get infinite fresh air due to being in the open.
FCs are useful because they keep the surface from drying out too fast, but the bag accomplishes the same thing and better. FCs allow the whole surface to fruit, and yellow oysters won't take advantage of that anyway. They like to put all their energy into growth from one, maybe two points.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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There's likely multiple phage' for each type of bacteria on the planet.
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hOoLiGaNNNNNNN
Stranger
Registered: 10/29/21
Posts: 2
Last seen: 2 years, 2 months
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Can this crop be saved? Been in fruiting conditions for about 11 days now. Pinning for about the last 5 days.
Lots of short stubby pins. The substrate doesn't seem all that wet, but if I over-mist I get some water pooling on the surface. Seems like I'm in a quandry.
First grow so any help is appreciated.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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I think it is diseased in some way, what is growing is very deformed. That or some seriously damaged genetics.
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