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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Will do, thanks.
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Hahaha..
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Cultivation General Discussion [Re: abductee]
#24141634 - 03/06/17 03:55 PM (6 years, 10 months ago) |
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P. Serbica, any successful indoor grows with it? So far the threads I've seen say it hasn't been done, but most of those threads were really old.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 16 hours, 16 minutes
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Re: Cultivation General Discussion [Re: abductee]
#24141821 - 03/06/17 05:23 PM (6 years, 10 months ago) |
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Am I an idiot for pasteurizing my coir/verm casing material after heat treating it and letting it cool? I have a feeling the answer may be yes.
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Shadowboxing the apocalypse and wandering the land
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Mr Piggy]
#24141823 - 03/06/17 05:29 PM (6 years, 10 months ago) |
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Quote:
Mr Piggy said: Or someone's got it for you, nice supporter flash.
Hey stare, are you trying to get the wood pellets for Psilocybe cyans? I know they can colonize Douglas-fir chips, maybe get a bag of pellets if they're cheap and do a test with them? No promises, never seen it done. 
Nah, Lion's Mane and other edibles where I need hardwood fuel pellets. It's driving me nuts!
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themadextractor
Stranger


Registered: 06/25/14
Posts: 230
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: Crispykoot]
#24141902 - 03/06/17 06:16 PM (6 years, 10 months ago) |
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Quote:
Crispykoot said: Am I an idiot for pasteurizing my coir/verm casing material after heat treating it and letting it cool? I have a feeling the answer may be yes.
It is unnecessary but may help break down the coir more than just bucket tek. some have speculated its easier for the mycelium to digest.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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You're not an idiot, but you're adding extra steps that don't justify the added time, IMO. You also don't make a casing layer with the intention if it being eaten by the myc, so themadextractor's point above shouldn't be considered much for casing.
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themadextractor
Stranger


Registered: 06/25/14
Posts: 230
Last seen: 1 year, 3 months
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Thanks. Missed the casing part.
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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I mixed a few grams of cellulase enzyme (ironically extracted from Trichnoderma), into my coir prep water right before adding to the bucket. Was hoping to see some sort of boost in yield due to coir being slightly more broken down for myc.
Didn't noticed anything at all... harvested average yield from the tub
Oh well. lol
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FriedEgg


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Quote:
Thedillestpickle said: cellulase enzyme (ironically extracted from Trichnoderma),
can you tell us the details of that? how and why would you do that?
edit: oh, i thought YOU extracted it from trich. i'm guessing you purchased it.
Edited by FriedEgg (03/06/17 09:53 PM)
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Enzymes are meant to function in a very specific temperature range based on their function and host, so it's very likely that even if it would have made a difference, it was probably denatured by the heat of the water.
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: natedawgnow]
#24142469 - 03/06/17 10:03 PM (6 years, 10 months ago) |
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Quote:
natedawgnow said: Enzymes are meant to function in a very specific temperature range based on their function and host, so it's very likely that even if it would have made a difference, it was probably denatured by the heat of the water.
Yea I tried doing a bit of research first and it seemed like it was stable for at least a while at high temp. Also it's more active at higher temp.
It's something that would probably take a bit more research to find if it has any viability. Maybe pH would be a factor as well.
I just did it for fun, but I was kinda hoping it would do something really cool like boost yield. Maybe I'll try it again adding it to the substrate during spawning.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Ya but no enzyme in a living cell would work at boiling temps for more than a few moments, especially not one derived from a simple mold. But mixing it into your sub may be different
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: natedawgnow]
#24142885 - 03/07/17 04:23 AM (6 years, 10 months ago) |
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For those who make plates from spore syringes,
A microscopy order usually comes with a Luer lock cap, a sterile needle, and the 10cc syringe. When putting a drop of solution to a plate, what's your protocol for storing the now-opened syringe?
It's been so long since I've used a syringe that I'm confusing myself. When I remove the cap to replace with the needle point, I'm going to wrap the cap in an iso-soaked paper towel. When I'm done dropping spores, I'm going to wipe down the cap thoroughly, and then quickly replace the needle with it again. This is standard, right?
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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The syringe didn't come with a needle cap as well? Luer lock syringes are pretty safe, the needle won't slip off accidentally, just make sure to screw the needle tightly all the way down and you won't need to take the needle off again after use. No contams are going to enter through the needle into the syringe even without a needle cap.
Edited by Josex (03/07/17 05:17 AM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Josex]
#24142951 - 03/07/17 05:53 AM (6 years, 10 months ago) |
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Quote:
Josex said: The syringe didn't come with a needle cap as well? Luer lock syringes are pretty safe, the needle won't slip off accidentally, just make sure to screw the needle tightly all the way down and you won't need to take the needle off again after use. No contams are going to enter through the needle into the syringe even without a needle cap.
Nah, no needle caps. Just the needle attachment and initial Luer plug. I didn't know that contams couldn't enter the syringe, that's awesome since that was my fear. I'll probably just leave the needle attached. Iff if it's not a blunt tip, I'll wrap it in foil so it doesn't stab the bag. Sweet.
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Gizmoleon
Stranger
Registered: 07/08/16
Posts: 23
Last seen: 6 years, 8 months
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Question: I made som pf jars with som leftover brown rice I had laying around. I used an ms syringe with Ecuador strain to nock them up, but after four days without sign of growth I got impatient and nocked them up again with an LC GT strain just for the hell of it. I know four days is early, but I thought I would experiment a little.
So, what will happen when/if I have two different species in one cake? Anyone tried?
-------------------- "Who says it's a Camberwell Carrot? I do. I invented it in Camberwell, and it looks like a carrot."
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: Gizmoleon]
#24142989 - 03/07/17 06:23 AM (6 years, 10 months ago) |
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Ecuador and GT are the same Species so nothing will happen except the LC will probably start colonizing before Ecuador germinates
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Cultivation General Discussion [Re: ComebackKid]
#24143954 - 03/07/17 01:56 PM (6 years, 10 months ago) |
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Stare thats pretty weird the needle is just open?? Usually it has a cap but if i were you i would wrap in foil and keep it flat in a ziplock in your drawer in the mini fridge if you have one if not keep it on a shelf in your fridge
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Boogieman47]
#24143962 - 03/07/17 01:57 PM (6 years, 10 months ago) |
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I knocked up cakes once with two different syringes.
Produced nothing but blobs. Was GT and mazatapec
-------------------- KSSS And PE WBS.
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