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Failboat
Fuck Up
Registered: 02/01/18
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Re: Cultivation General Discussion [Re: TedsDead]
#27509612 - 10/18/21 10:02 PM (2 years, 3 months ago) |
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 I need to get a jewelers loop, i took transfers, but the leading edge looks like birdsnest formation. These plates were from 10/3
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Doctor Mario
πππππ



Registered: 08/07/20
Posts: 1,894
Loc: πΊπΈ
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Re: Cultivation General Discussion [Re: Failboat]
#27509625 - 10/18/21 10:13 PM (2 years, 3 months ago) |
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That's either myc or mold that's really trying to stay hidden but I'd bet it's just myc. I've put plates with spiky edges on grain and everything went fine.
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Failboat
Fuck Up
Registered: 02/01/18
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Re: Cultivation General Discussion [Re: Doctor Mario]
#27509662 - 10/18/21 11:26 PM (2 years, 3 months ago) |
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Depending on the look of these transfer plates I may just noc a small jar and try to at least get a clone candidate
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
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Re: Cultivation General Discussion [Re: Failboat]
#27509694 - 10/19/21 12:52 AM (2 years, 3 months ago) |
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Ahem...Optics nerd here. Been a minute since I looked at those diopters;but lets say the average loupe has a working distance of 5CM. So you need to be 5cm from the sample for a clear image.
20D loupe: 1/20=0.05m (2inches I think, can we please just go metric)
If you want to drop money on a lenses. Look up double aspheric.
Baby jars for all those strange tommy plates for me. You can even sprinkle a layer of grain right on top of that plate seal it up and watch it eat grain...
Edited by rockyfungus (10/19/21 01:01 AM)
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JXAllen
Cannalyst


Registered: 08/06/21
Posts: 615
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Re: Cultivation General Discussion [Re: Failboat]
#27509698 - 10/19/21 12:59 AM (2 years, 3 months ago) |
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Quote:
Quirkmeister92 said:
 I need to get a jewelers loop, i took transfers, but the leading edge looks like birdsnest formation. These plates were from 10/3
I recently had a couple cultures of Pink Buffalo I transferred from spores. They stayed tomentose every transfer. But once I added it to millet, it started showing some rhizomorphic growth. I've read that it's not uncommon and tomentose isn't bad. It's just easier to recognize the rhizomorphic growth for what it is.
  
It was also the first og my shoe boxes to show life, nice feathery rhizomorphic growth.
-------------------- There are only 10 types of people in the world, those that understand binary and those that do not. Cultures I'm workin on
 
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: rockyfungus]
#27509706 - 10/19/21 01:27 AM (2 years, 3 months ago) |
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Quote:
rockyfungus said: Ahem...Optics nerd here. Been a minute since I looked at those diopters;but lets say the average loupe has a working distance of 5CM. So you need to be 5cm from the sample for a clear image.
20D loupe: 1/20=0.05m (2inches I think, can we please just go metric)
If you want to drop money on a lenses. Look up double aspheric.
Baby jars for all those strange tommy plates for me. You can even sprinkle a layer of grain right on top of that plate seal it up and watch it eat grain...
I dont see what the problem is tho? its pretty easy to get a clear image of cultures with the loupe. I have several different ones. looking through one now Id say its about 2 cm for a clear image
all Im finding for double aspheric is eyeglasses. u got a cool loupe alternative thats double aspheric?
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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QM33
(NOT A PUPPET!) β€β€β€β€β€



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: TedsDead]
#27509844 - 10/19/21 06:57 AM (2 years, 3 months ago) |
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So what is this optic loop thing and do i need one.
Ape has always been rrally thick tom like that for me, unless you really try to isolate somer rhizo, which ive done.
Another thing ive found is that alot of my strains go to with a lighter agar mix in the plate. Ive been doing 16.7g agar to 1000ml and all my strains seem much more tom
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rockyfungus
dirty


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Re: Cultivation General Discussion [Re: QM33]
#27509913 - 10/19/21 08:41 AM (2 years, 3 months ago) |
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It's not needed at all. Just a fun toy. A 20D aspheric lens is corrected for field curvature and aberrations. Essentially I have a wider field of view and I can move my wrist and head to move my view seamlessly and in all the planes clearly. Small small movements made those "contams" or disease POP...
When they exam your eyes they basically jump in your eye through a small opening and then get a huge view that needs to wrap around a ball to almost see at the front of the ball.
 See how much light scatters anywhere but the center of a normal spherical lens. With asphere you can look anywhere and get a true image. Shitty lenses have an optical center and that's only spot it's really clear.
Edited by rockyfungus (10/19/21 10:42 AM)
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Enjoil



Registered: 10/29/20
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Re: Cultivation General Discussion [Re: Failboat]
#27509940 - 10/19/21 09:09 AM (2 years, 3 months ago) |
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Hey bro, I had a melmack germ plate that looked kinda wonky like that. I didnβt transfer for a while cuz I thought it was going to start turning colors. It never did.
I eventually popped it open and took 5 transfers. I smelled it and it smelled ok. Hopefully the growth gets less wonky when it grows out some. I will take some pictures if you want to see that melmack plate.
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QM33
(NOT A PUPPET!) β€β€β€β€β€



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: Enjoil]
#27509957 - 10/19/21 09:19 AM (2 years, 3 months ago) |
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So one of those lenses hes demonstrating could be used to identify contam in petri dishes?
Compared to what? What do u use Ted?
These are reccommended/somewhat necessary?
Never seen someone using magnificatuon to try and verify cultures..
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rockyfungus
dirty


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Re: Cultivation General Discussion [Re: QM33]
#27510036 - 10/19/21 10:30 AM (2 years, 3 months ago) |
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Here just to showcase. Your own eyes are better and being able to manipulate light direction is huge.
Photos are very impossible to use while holding a light and lens...


If I did this right, first is normal lens with mag. Second image is me being able to look at where myc meets mold instead just a flat image. More or less I'm using the light and angle of lenses to see elevation and tiny tiny things you really can't see any other way. Not needed, but someone gave me a tool that they didn't need and it fit a need.
Considering I saw a 3d image with my eyes and now it's squashed on a screen. Just look at the shadows and it can give you a sense. Last image is super blurred at 1-6 o'clock cuz the lens is tilted back that way to focus all the light where slime hits myc.
Edited by rockyfungus (10/19/21 10:45 AM)
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smalltalk_canceled
Babnik


Registered: 07/13/20
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Re: Cultivation General Discussion [Re: rockyfungus]
#27510225 - 10/19/21 01:12 PM (2 years, 3 months ago) |
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Never fucking seen a bucket lid in a store or anywhere else at all in my entire life

They really exist for round buckets?
-------------------- Willpower is the one true virtue
  
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Guerrilla
Bumbaclart


Registered: 01/30/21 
Posts: 3,170
Loc: United Kingdom
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Quote:
smalltalk_canceled said: Never fucking seen a bucket lid in a store or anywhere else at all in my entire life

They really exist for round buckets?
Lol I found the same, are you in Europe? Check Amazon for βbucket with lidβ.
-------------------- Being pissed on does not make you a real man.
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smalltalk_canceled
Babnik


Registered: 07/13/20
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Re: Cultivation General Discussion [Re: Guerrilla]
#27510266 - 10/19/21 01:47 PM (2 years, 3 months ago) |
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Let's pay the Americas to send us some.
-------------------- Willpower is the one true virtue
  
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 14 hours, 27 minutes
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You guys don't sell the lids for buckets? Ask a restaurant for some or do yall not have buckets o food?
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: rockyfungus]
#27510298 - 10/19/21 02:25 PM (2 years, 3 months ago) |
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Quote:
rockyfungus said: It's not needed at all. Just a fun toy. A 20D aspheric lens is corrected for field curvature and aberrations. Essentially I have a wider field of view and I can move my wrist and head to move my view seamlessly and in all the planes clearly. Small small movements made those "contams" or disease POP...
When they exam your eyes they basically jump in your eye through a small opening and then get a huge view that needs to wrap around a ball to almost see at the front of the ball.
 See how much light scatters anywhere but the center of a normal spherical lens. With asphere you can look anywhere and get a true image. Shitty lenses have an optical center and that's only spot it's really clear.
ooh, cool.
yeah, definitely dont need a loupe bit they are fun toys. great for spotting pests on plants and looking at trichomes too
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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foragedfungus



Registered: 09/30/13
Posts: 1,849
Loc: out there
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Re: Cultivation General Discussion [Re: TedsDead] 1
#27510332 - 10/19/21 02:57 PM (2 years, 3 months ago) |
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You can hold the loupe right up to your phone camera for some cool, super close-up zoom photography. Here's the pore surface of a Fistulina hepatica I found the other day, through a 30x loupe.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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ooh, that is super cool
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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smalltalk_canceled
Babnik


Registered: 07/13/20
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Re: Cultivation General Discussion [Re: TedsDead]
#27510415 - 10/19/21 04:10 PM (2 years, 3 months ago) |
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Doing what I believe to be a actually useful test tonight, checking if I can add dry buck wheat for human consumption straight to hydrated corn, and still sterilize it properly.
Would allow you to dry - and bulk up your hydrated grain.
To non corn growers this may seem silly, but there's a lot heat and pressure needed to soften the kernel compared to a lot of cold soaks and other grains when you have to properly hydrate corn.
Takes more time and more energy.

5 jars - two corn buck wheat, three corn corn tortilla chips as a comparison
-------------------- Willpower is the one true virtue
  
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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