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GoldenBoy
Stranger
Registered: 10/19/20
Posts: 17
Last seen: 1 year, 4 months
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Re: Cultivation General Discussion [Re: Failboat]
#27492504 - 10/04/21 03:46 PM (2 years, 3 months ago) |
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Trying to find a good casing recipe... whatcha got?
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 12 hours
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Re: Cultivation General Discussion [Re: GoldenBoy]
#27492536 - 10/04/21 04:01 PM (2 years, 3 months ago) |
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Lime coir verm?
-------------------- Willpower is the one true virtue
  
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deadmandave
Slime


Registered: 02/16/10
Posts: 3,355
Loc:
Last seen: 2 hours, 16 minutes
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Doesn't peat moss make a better casing than coir?
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: deadmandave]
#27492629 - 10/04/21 05:25 PM (2 years, 3 months ago) |
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peat verm lime gypsum
peat:verm 50/50, gypsum @10% dry weight. lime until PH is 8-8.5
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: GoldenBoy]
#27492681 - 10/04/21 06:38 PM (2 years, 3 months ago) |
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Quote:
GoldenBoy said: Trying to find a good casing recipe... whatcha got?
https://www.shroomery.org/forums/showflat.php/Number/27177370#27177370
Faht
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 12 hours
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Re: Cultivation General Discussion [Re: fahtster] 1
#27492772 - 10/04/21 08:00 PM (2 years, 3 months ago) |
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Temps are rising above 10C and seems to make whatever this is stall
3 dead, 1 cloned (attempt)
2 pins still alive
-------------------- Willpower is the one true virtue
  
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Lemgrub



Registered: 04/02/18
Posts: 589
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Re: Cultivation General Discussion [Re: Crackatoa]
#27492807 - 10/04/21 08:18 PM (2 years, 3 months ago) |
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Quote:
Crackatoa said: How do you fight the little fucker's?
I somewhat recently found a method that's working. Store all my media in new ziplocks or a ~16qt container that I mist with bleach water in between uses. Use only natedawg style lids for glass jars, my LC jars are 250ml ziplock PP plastic jars that I keep in ziplocks. Pretty much it, just check your petris periodically for the condensation trails and to see if myc is eaten away. They haven't been a problem since.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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GoldenBoy
Stranger
Registered: 10/19/20
Posts: 17
Last seen: 1 year, 4 months
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Re: Cultivation General Discussion [Re: fahtster]
#27492869 - 10/04/21 08:44 PM (2 years, 3 months ago) |
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Quote:
fahtster said:
Quote:
GoldenBoy said: Trying to find a good casing recipe... whatcha got?
https://www.shroomery.org/forums/showflat.php/Number/27177370#27177370
Faht
Thanks Faht! I was hoping someone would chime in with a nice writeup
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: GoldenBoy]
#27492916 - 10/04/21 09:18 PM (2 years, 3 months ago) |
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Np.. I’m literally making some rn lol

Faht
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Feedyourhead33
Stranger

Registered: 06/04/21
Posts: 20
Last seen: 5 months, 30 days
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Re: Cultivation General Discussion [Re: fahtster]
#27493143 - 10/05/21 03:01 AM (2 years, 3 months ago) |
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I’m looking for some advice. I’ve been having practically everything contam on me recently. I’m new to this. I had a successful first run of MS to BRF cakes to CVG shoeboxes. Started doing agar with some pasty plates. Took some clones from splitting a stem and scraping a little bit of tissue. I even got lucky with some LC from spore. Then I started seeing some contams. Trich I think was the first I saw. At this point I’ve seen a bunch of different stuff. Bacterial and random mold. I learned a lot of lessons from trial and error. Like I couldn’t get WBS to the correct moisture level so all the time that the myc wasn’t colonizing allowed for for contams to take hold. I also decided to try some bags thinking all the jars needed for a monotub would be annoying but realized after slow colonization from agar drops and G2g that I was over filling the bags and not PC’ing long enough so the millet wasn’t sterilized. So through trial and error I figured out what I was doing wrong with those. I also had some hidden contams on agar I used on a couple jars of LI. The pasty plates looked clean but it is pretty hard to see through them. A couple times I didn’t know a plate was no good until I started cutting wedges. Should I switch to pouring petris? Pouring in the SAB seemed like a pain but if I can never tell if it’s clean the no pours is useless. So I learned a bunch of lessons from trial and error but my contamination issues keep persisting. My agar Was going decent at first with contams only showing up after a long time but now it’s like every time I transfer something funky shows up pretty quickly. I haven’t had a clean plate that was useable in a while at this point. I do transfers get some healthy looking growth and before I have a free night to dedicate to agar work contams start showing up.
So I’m brushing up on my sterile technique. My SAB was small at first so it made it hard to use but I got a bigger tub. I’m thinking the arm holes may actually be too small. I was just reading if there’s not enough airspace around your arms you actually make a lot of air movement with your movements. So I guess my question is where should I be looking to correct my issues. Obv properly hydrated grain sterilized long enough is key to giving the myc the best chance of taking hold quickly. I’m reviewing all my technique and going start transferring agar as soon as I get a couple days growth of myc. I’ve cleaned the room a couple times but I’m going to give it another deep clean. At one point I noticed my window AC units air intake looked dirty so I cleaned the whole thing including the filter well. I turn the AC off 15-30 min before I do any inoculating or transfers in the sab. What else would you do if you were having my issues? I got the arm holes of the sab I’m looking into. I’m confused by that because I thought I saw a couple people even make plastic flaps so your arms basically make a seal. Wouldn’t that be bad if your supposed to have some space around your arms? Is the iso rag a good enough cleaner for my hands/arms? I was spraying ISO for a while but just read that I should be using the soaked rag for 30 seconds for it to be effective. How often do you clean your hands/arms when your coming in and out of the sab to flame sterilize? I use iso or bleach spray to clean the SAB and table before use and make sure all surfaces are wet with soapy water before I start. Any tips would be appreciated
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,959
Loc: Hyperspace Chicken Coop
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arm holes are definitely a major when working with a SAB, make them at least 7" wide. I reapply ISO when my gloves starting to get dry. I use a spray bottle to soak my gloves.
Slow but steady movements with wide arm holes and watch some videos of people working in a SAB, see if that does improve your issues.
There is more to look at but sort this out first.
link to bod's SAB videos: https://www.shroomery.org/forums/showflat.php/Number/24337011#24337011
Edited by Baba Yaga (10/05/21 04:27 AM)
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897
Number


Registered: 08/20/21
Posts: 60
Loc: Out of Bounds
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Re: Cultivation General Discussion [Re: Guerrilla]
#27493155 - 10/05/21 03:37 AM (2 years, 3 months ago) |
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Quote:
Guerrilla said:
Quote:
897 said:
Quote:
sandman420 said: maybe insects or slugs or some shit
Yeah, i had a sample spawn fruit fly maggots, before they were big enough to see, i saw trails all over the lid.
Well shit, maybe it was that then.
Are you going to let them grow? I quarantined the plate when i found it and finally saw some large enough after four days or so. Pretty neat of you ask me. I think they must have come from the spore syringe i used in my case(i don't have any luck with syringes anyway).
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Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: fahtster]
#27493174 - 10/05/21 04:22 AM (2 years, 3 months ago) |
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Quote:
fahtster said: Np.. I’m literally making some rn lol

Faht
Gas is getting more expensive where I live, I wonder if the same technique could be done in a Bain marie / water bath in the oven, i like the idea of it being contained in the bag and potentially you could control the temp better in the oven if your thermostat is any good…I think I’m gonna give it a whirl anyways…
-------------------- Back once again with the ill behaviour Links I like
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Guerrilla
Bumbaclart


Registered: 01/30/21 
Posts: 3,170
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: 897]
#27493205 - 10/05/21 05:18 AM (2 years, 3 months ago) |
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Quote:
897 said:
Quote:
Guerrilla said:
Quote:
897 said:
Quote:
sandman420 said: maybe insects or slugs or some shit
Yeah, i had a sample spawn fruit fly maggots, before they were big enough to see, i saw trails all over the lid.
Well shit, maybe it was that then.
Are you going to let them grow? I quarantined the plate when i found it and finally saw some large enough after four days or so. Pretty neat of you ask me. I think they must have come from the spore syringe i used in my case(i don't have any luck with syringes anyway).
Well, they weren't petri dishes. They were 33qt plastic tubs I purchased, so I can't do jack shit about it. They'll be all over the place already if that's what it was. But I'm hoping it wasn't. They're quite large trails for mites.. so maybe not. I've got a 60-120x pocket scope coming today, I'll check around everything as much as possible.
I set a few jars of colonised grass seed around the tubs to see if it'll attract any.
-------------------- Being pissed on does not make you a real man.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Quote:
Feedyourhead33 said: I’m looking for some advice. I’ve been having practically everything contam on me recently. I’m new to this. I had a successful first run of MS to BRF cakes to CVG shoeboxes. Started doing agar with some pasty plates. Took some clones from splitting a stem and scraping a little bit of tissue. I even got lucky with some LC from spore. Then I started seeing some contams. Trich I think was the first I saw. At this point I’ve seen a bunch of different stuff. Bacterial and random mold. I learned a lot of lessons from trial and error. Like I couldn’t get WBS to the correct moisture level so all the time that the myc wasn’t colonizing allowed for for contams to take hold. I also decided to try some bags thinking all the jars needed for a monotub would be annoying but realized after slow colonization from agar drops and G2g that I was over filling the bags and not PC’ing long enough so the millet wasn’t sterilized. So through trial and error I figured out what I was doing wrong with those. I also had some hidden contams on agar I used on a couple jars of LI. The pasty plates looked clean but it is pretty hard to see through them. A couple times I didn’t know a plate was no good until I started cutting wedges. Should I switch to pouring petris? Pouring in the SAB seemed like a pain but if I can never tell if it’s clean the no pours is useless. So I learned a bunch of lessons from trial and error but my contamination issues keep persisting. My agar Was going decent at first with contams only showing up after a long time but now it’s like every time I transfer something funky shows up pretty quickly. I haven’t had a clean plate that was useable in a while at this point. I do transfers get some healthy looking growth and before I have a free night to dedicate to agar work contams start showing up.
So I’m brushing up on my sterile technique. My SAB was small at first so it made it hard to use but I got a bigger tub. I’m thinking the arm holes may actually be too small. I was just reading if there’s not enough airspace around your arms you actually make a lot of air movement with your movements. So I guess my question is where should I be looking to correct my issues. Obv properly hydrated grain sterilized long enough is key to giving the myc the best chance of taking hold quickly. I’m reviewing all my technique and going start transferring agar as soon as I get a couple days growth of myc. I’ve cleaned the room a couple times but I’m going to give it another deep clean. At one point I noticed my window AC units air intake looked dirty so I cleaned the whole thing including the filter well. I turn the AC off 15-30 min before I do any inoculating or transfers in the sab. What else would you do if you were having my issues? I got the arm holes of the sab I’m looking into. I’m confused by that because I thought I saw a couple people even make plastic flaps so your arms basically make a seal. Wouldn’t that be bad if your supposed to have some space around your arms? Is the iso rag a good enough cleaner for my hands/arms? I was spraying ISO for a while but just read that I should be using the soaked rag for 30 seconds for it to be effective. How often do you clean your hands/arms when your coming in and out of the sab to flame sterilize? I use iso or bleach spray to clean the SAB and table before use and make sure all surfaces are wet with soapy water before I start. Any tips would be appreciated
I’ll second making bigger arm holes and trying pasty plates again. Sure the condensation makes it hard to see through the sides but you can shine a light through the lid and look at the plate through the bottom of the plate. Bacteria is obvious and can be tossed. Mycelium can be difficult to distinguish, but can be done. If you open up a contamed plate in your sab then you should respray and wait a few minutes before working in there again, because if you don’t the spores will most likely make their way onto the next plate since there isn’t anywhere else for them to go
-------------------- Life’s shit, but I’m loving it
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 7 hours, 10 minutes
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Re: Cultivation General Discussion [Re: Wall.E]
#27493266 - 10/05/21 07:26 AM (2 years, 3 months ago) |
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Does anybody pc water inside syringes??
I did it the other day and half were fine and half were empty, not sure what causes it. Thinking maybe tiny amounts of air.
When I google all I get is results for a science experiment where you make water boil inside the syringe by pulling the plunger super fast
--------------------
LAGM2020     
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Guerrilla
Bumbaclart


Registered: 01/30/21 
Posts: 3,170
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: A.k.a]
#27493281 - 10/05/21 07:43 AM (2 years, 3 months ago) |
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Yea only ever lose half a ml, needle pointing up?
-------------------- Being pissed on does not make you a real man.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: Guerrilla]
#27493293 - 10/05/21 07:59 AM (2 years, 3 months ago) |
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You can also put the needles in a jar of water… always comes out with no water loss.. when the pressure pushes the water out, it just sucks water back up when it cools.. or it never loses water.. not sure exactly what goes on in there lol
Faht
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 7 hours, 10 minutes
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Re: Cultivation General Discussion [Re: fahtster]
#27493328 - 10/05/21 08:34 AM (2 years, 3 months ago) |
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I actually put like 20 in a filter bag and had to put them at a 45 degree angle to fit cuz it was in an instant pot.
I’ll pay attention next time and try to get them all needle side up. I like the jar idea too.
--------------------
LAGM2020     
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: A.k.a]
#27493336 - 10/05/21 08:42 AM (2 years, 3 months ago) |
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Don’t tell anyone else this but I usually just stick them in the trivet holes in the bottom of my pc so my syringes use pc water I just have a 16qt presto.. full syringes don’t fit upright in a jar… but when I had 23qt pc’s, they fit
I use all my syringes right away so I’m not worried that it’s pc water. I think the jar would actually fit in a 16qt if I didn’t have lid bands raising the trivet but I’m usually pc’ing 4oz jars with the syringes and qts so I have to use the lid bands
Faht
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