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OfflineQuirkmeister92
Street Doctor
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Registered: 02/01/18
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Re: Cultivation General Discussion [Re: joze]
    #27483848 - 09/27/21 02:22 PM (3 months, 26 days ago)

:freakout:


--------------------
Team wheat...
Quirks Tub Prep


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
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Re: Cultivation General Discussion [Re: Quirkmeister92]
    #27483864 - 09/27/21 02:41 PM (3 months, 26 days ago)

Anything wrong with this p mexicana plate?



Is it inadvisable to take this to grains?


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Willpower is the one true virtue



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OfflineSmoothcat
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27483907 - 09/27/21 03:25 PM (3 months, 26 days ago)

Quote:

joze said:
That feeling when you go on vacation and have to leave your cultures behind...




I feel your pain

:doingmediocre:

Quote:

smalltalk_canceled said:
Anything wrong with this p mexicana plate?





Is it inadvisable to take this to grains?




Personally I’d do another transfer. You’ve got growth right to the edge of the plate and not sure what’s going on at 6 o’clock…although it’s hard to see, if it’s clean then sure go to grain but if it were me I’d do another transfer, why waste your grain


--------------------
Back once again with the ill behaviour



Links I like


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OfflineOra
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Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 1 month, 17 days
Re: Cultivation General Discussion [Re: Smoothcat]
    #27484620 - 09/28/21 02:23 AM (3 months, 26 days ago)

i misted with alcohol instead of water on accident :facepalm3:
the sub bled blue for a bit. it was interesting to see pure blue drops of liquid.


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Offlinerockyfungus
dirty
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Registered: 03/01/21
Posts: 727
Loc: Front range
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Re: Cultivation General Discussion [Re: Ora]
    #27484652 - 09/28/21 03:55 AM (3 months, 26 days ago)

On topic of DIY induction heater/bactinator.

Search induction heater, fuck combustion, and or dynavap.
Been years since I read about it.

https://fuckcombustion.com/threads/diy-induction-heater-builds-and-references.49760/


--------------------
LAGM 2022


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Offlinesmalltalk_canceled
Babnik
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Re: Cultivation General Discussion [Re: rockyfungus] * 2
    #27484702 - 09/28/21 06:11 AM (3 months, 26 days ago)



--------------------
Willpower is the one true virtue



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OfflineGuerrilla
Monanacahuia


Registered: 01/30/21
Posts: 1,093
Loc: United Kingdom Flag
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27484773 - 09/28/21 08:47 AM (3 months, 26 days ago)

Plates in the fridge will build up water, reckon if I bought a mini fridge and turned the temperature down it’d keep them good for a while without the water?


--------------------


Yup it’s me again. Shit, I post too much..

fahtster said:
The only bad experience is the one not had.


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InvisibleModularMind
M.P.F.
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Registered: 02/09/10
Posts: 6,884
Re: Cultivation General Discussion [Re: 0ptiquest]
    #27484819 - 09/28/21 09:50 AM (3 months, 26 days ago)

Quote:

0ptiquest said:
The scalpel blade turns red in less than 10 seconds, i love my zapper.





No doubt it’s convenient to place it in there and have it ready, but 10 seconds in the open flame of an alcohol lamp does the same.
Certainly something to have at a commercial scale though.


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Invisiblesandman420
Saint PP
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Registered: 06/17/04
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Re: Cultivation General Discussion [Re: ModularMind] * 1
    #27484843 - 09/28/21 10:18 AM (3 months, 26 days ago)

They build water from temp swings. Cheap little fridges are bad at this, especially if they have a freezer in them.

You could buy a lab fridge for cheap on ebay if you look around or build one super easily out of a chest freezer. Simply plug the chest freezer in to a PID temp controller with a temp probe in the freezer and set it to your desired temp, 6c/42f apparently is a good temp. Program the PID to keep a 1 degree difference in between kicking on and make sure to have a minimum of 3 minutes between cycles or you can burn the compressor up. Just have the normal switch of the freezer set to max so it will always run full blast when the PID tells it to.

Make sure that you autotune the PID and you run it for several days to make sure it is good and stable before you put valuable cultures in there.

Add thermal stores to your setup, keep them in soft coolers inside can help too.


--------------------
Have no fear of perfection, you'll never reach it.


Edited by sandman420 (09/28/21 10:24 AM)


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OfflinePrimalSoup
It's psychedelically valid
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Posts: 13,060
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Re: Cultivation General Discussion [Re: smalltalk_canceled] * 2
    #27485231 - 09/28/21 04:32 PM (3 months, 25 days ago)

Quote:

smalltalk_canceled said:
Trich isnt a contamination,it's art:


https://trendland.com/when-mold-is-looking-fabulous-the-microorganisms-by-dasha-plesen/




If I add a few drops of food coloring before the agar sets then I can sell NFTs? :laugh2:



--------------------


if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: LAGM2022! Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 


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OfflineA.k.a
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Registered: 10/27/19
Posts: 14,328
Loc: Gaming the system
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Re: Cultivation General Discussion [Re: PrimalSoup]
    #27485402 - 09/28/21 06:29 PM (3 months, 25 days ago)

Shit I’ll screenshot your post and sell that as nft :lol:


Whatever it is there’s somebody out there to buy it.


--------------------
LAGM2020


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: A.k.a]
    #27486777 - 09/29/21 09:16 PM (3 months, 24 days ago)

I have been beating my head against the wall for months dealing with an insane rate of contamination. Nothing I have tried has worked. I was starting to lose all confidence in my ability to grow mushrooms.

I pc'd 18 bags of oats a week and a half ago. I never had the chance to inoculate them as I have been super busy baking and cooking for my sisters wedding. I went and checked on my bags of oats a few minutes ago. The oats were sterilized in my 75x at 20 psi for 3 hours. After they cooled I took them to my lab and placed them on a shelf where they have sat ever since. I just discovered 4 of them with visible contamination. These bags were sterilized pre sealed. They are still sealed. How can this happen? Should I expect the rest to eventually contaminate also?



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InvisiblemushboyMDiscord
moooooooosh
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Registered: 04/24/05
Posts: 26,636
Loc: better trashcan
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Re: Cultivation General Discussion [Re: BrownBear]
    #27486781 - 09/29/21 09:20 PM (3 months, 24 days ago)

Any holes? Submerge in water see if you get bubbles:justdontknow:


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: mushboy]
    #27486784 - 09/29/21 09:22 PM (3 months, 24 days ago)

Sound idea. Thanks, mushboy.


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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 3,794
Loc: Oregon
Re: Cultivation General Discussion [Re: BrownBear]
    #27486787 - 09/29/21 09:25 PM (3 months, 24 days ago)

Vent for 20 min? How big are they? Spaced? May need to go longer.. I never had any luck with 3 hours, i do 3.5 at least. Been doing 5 last couple runs, may be overkill


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: QM33]
    #27486799 - 09/29/21 09:38 PM (3 months, 24 days ago)

I vent for 20 minutes or longer everytime. But that doesn't explain mold contamination with uninoculated sterilized grain bags. Bacteria endospores could survive if the sterile process isn't complete but mold is not surviving those temps.

Anyway, I submerged one of the bags and I got no bubbles. I decided to squeeze the bag while it was submerged and air bubbles began to protrude from a few spots on the bag. I lifted the bag and searched those areas but cannot visibly see or feel any holes. This is fucking weird but could explain my contamination issues at large. I think I might make the switch to jars.


Edited by BrownBear (09/29/21 09:38 PM)


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Invisiblep9hu7
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Re: Cultivation General Discussion [Re: BrownBear]
    #27486812 - 09/29/21 09:49 PM (3 months, 24 days ago)

You're exactly right, mold will not survive the cycle which means your contamination is occurring post cycle.
This rarely happens to me but it's always when I disturb the mouth of the bag enough to break the seal.

Are you using unicorn bags or a knock-off?


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InvisibleQM33
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Registered: 04/09/20
Posts: 3,794
Loc: Oregon
Re: Cultivation General Discussion [Re: BrownBear]
    #27486816 - 09/29/21 09:51 PM (3 months, 24 days ago)

That is a good point... I'm sure your guage not broken and you know how to read a rocker if it is.
Do you know if all 4 were in the same cooker or?

Another thing I was going to say, and I know it might sound crazy, but your oats look dry ha idk. Qnd if that were the case it could be a improper sterilization. But you checked the hydration also?


They're presealed uninoculated


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: p9hu7]
    #27486820 - 09/29/21 09:55 PM (3 months, 24 days ago)

I get my bags from a shroomery sponsor. I have used them in the semi distant and relatively recent past without issue. It has only been within the last 4 months that my contamination rate has skyrocketed. I typically inoculate within 2 days after sterilizing. These particular bags have been left undisturbed for a week and a half.


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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 3,794
Loc: Oregon
Re: Cultivation General Discussion [Re: BrownBear]
    #27486822 - 09/29/21 09:56 PM (3 months, 24 days ago)

I mean you guys are saying it yourself but yet we are looking directly at it.
How can mold survive those temps?
It must be improperly hydrated.


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