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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Crackatoa] 1
#27481176 - 09/24/21 08:07 PM (2 years, 4 months ago) |
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Just start over if the culture keeps contaminating on agar. Have you fruited it?
Unless it's a workhorse culture with top notch potency I say ditch it and start new rather than fuck with a tammy culture for over a year.
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 14 hours
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Re: Cultivation General Discussion [Re: Crackatoa]
#27481178 - 09/24/21 08:07 PM (2 years, 4 months ago) |
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You know this type of shit. Trying to tease whatever genetics were in a reddit "sticker". Can't believe people paid for this shit.
 That was a month ago so, plates worse now LOL I'm stubborn and want this damn KSSS to fruit and print.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: rockyfungus]
#27481183 - 09/24/21 08:21 PM (2 years, 4 months ago) |
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That looks more like cultivator error. Contam sprinkled all over the plate like that is pretty tell tale bad technique.
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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 12 hours, 52 minutes
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Re: Cultivation General Discussion [Re: natedawgnow]
#27481198 - 09/24/21 08:51 PM (2 years, 4 months ago) |
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How long can agar sit solidified in an agar bottle before you decide to make new agar? I have had some jar sitting with agar for like 2-3 months now, start over?
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SpaceBaby
alchemist



Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
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Re: Cultivation General Discussion [Re: rockyfungus]
#27481240 - 09/24/21 09:39 PM (2 years, 4 months ago) |
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Quote:
rockyfungus said: Just started working under a hood. I have some plates I've been working in a SAB for almost a year that just won't stop triching out.
Could I run the hood for a few hours then shut the flow and work in open air to do a transfer? Or best just to keep going in the SAB?
i run my FFU 24/7. figure it keeps the room cleaner.
-------------------- SpaceBaby SPACEBABY'S LAGM22 THREAD MUSHBOY'S SHROOM TEA TEK Me as a cube
Another Day's Work:
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: SpaceBaby]
#27481276 - 09/24/21 10:22 PM (2 years, 4 months ago) |
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Do HEPA filters eventually get clogged up?
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 14 hours
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Re: Cultivation General Discussion [Re: natedawgnow]
#27481284 - 09/24/21 10:34 PM (2 years, 4 months ago) |
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Quote:
fahtster said: Do HEPA filters eventually get clogged up?
They quoted me 7 years with 24/7 use. I'm not really comfortable running it 24/7 as doors are going to open and the AC/heat is going to cycle.
Quote:
natedawgnow said: That looks more like cultivator error. Contam sprinkled all over the plate like that is pretty tell tale bad technique.
At first I thought it was operator error. I'm not new to my lab, cultures, or agar. Seems I got bombed between a combination of dirty stickers, summer, and a dirty lab. As I refined my technique and went back to prints I kept contaming. All my clean cultures or known clean prints are still clean.
Some may be my error but when a single print out of 10 keeps showing the same germ...
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: fahtster]
#27481311 - 09/24/21 11:13 PM (2 years, 4 months ago) |
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Quote:
fahtster said: Do HEPA filters eventually get clogged up?
Yes it is called final pressure drop. Typically it is twice the starting pressure.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: rockyfungus] 1
#27481334 - 09/24/21 11:59 PM (2 years, 4 months ago) |
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Quote:
rockyfungus said:
Quote:
fahtster said: Do HEPA filters eventually get clogged up?
They quoted me 7 years with 24/7 use. I'm not really comfortable running it 24/7 as doors are going to open and the AC/heat is going to cycle.
Quote:
natedawgnow said: That looks more like cultivator error. Contam sprinkled all over the plate like that is pretty tell tale bad technique.
At first I thought it was operator error. I'm not new to my lab, cultures, or agar. Seems I got bombed between a combination of dirty stickers, summer, and a dirty lab. As I refined my technique and went back to prints I kept contaming. All my clean cultures or known clean prints are still clean.
Some may be my error but when a single print out of 10 keeps showing the same germ...
Ya but it's all over the plate, not just where the culture is.
How can it be the culture when contams are growing in spots on the plate that the culture isnt touching?
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: sandman420]
#27481433 - 09/25/21 06:00 AM (2 years, 4 months ago) |
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Quote:
A.k.a said: I’ve done a couple moldy plates at the end of a SAB session where I turn it off and do the transfer real quick and it’s been ok.
Idk why for any reason you would turn it off. The flow is suppose to blow contams backwards. If you turn it off and then open a contaminated player I'm sure it would spread everywhere.
Quote:
Crackatoa said: Done the same before and even did one last night.
So now you wait 2 weeks to make sure its clean? Even then contams can be sneeky..
Quote:
sandman420 said:
Quote:
fahtster said: Do HEPA filters eventually get clogged up?
Yes it is called final pressure drop. Typically it is twice the starting pressure.
So is that when you might worry about contams coming through? Or will they ever? Or will the flow rate just slow, and maybe even filter better because the pore size is becoming smaller as it clogs?
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: QM33]
#27481438 - 09/25/21 06:15 AM (2 years, 4 months ago) |
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 14 hours
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Re: Cultivation General Discussion [Re: natedawgnow]
#27481449 - 09/25/21 06:48 AM (2 years, 4 months ago) |
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Quote:
natedawgnow said:
How can it be the culture when contams are growing in spots on the plate that the culture isnt touching?
Streaked culture from a syringe. The whole plate was touched by an inoculation loop and most likely contaminated water. I've grown a ton this past year and never had these issues. Any of these reddit "stickers" and I've seen colors I didn't know was possible. I just looked through all these plates that contammed. They all show white with green coming off of it. So the culture is touching the trich on most plates.
If pouring plates in my SAB and letting them sit for months shows no contams is it really my tek? If swiping those said plates with no spores leads to clean plates is it really my tek?
Not calling you out or saying your wrong and not trying to be defensive. I've fixed my tek in the past and constantly evolved for my environment. I've started exotics and gourmets with no problems at all. Whenever I get contams it's maybe 0.1% and a quick review of the process reveals a mistake.
I've been using the same swab from a trusted member here for over a year with no issues. Not trying to blame reddit or anyone else, just want to figure out if I'm just wasting my time with a shit culture or if it's some crazy small error I'm overlooking.
My wife and myself are both trained in aspetic technique. She's watched over me and has seen no issues. I've had her do the work as she thrives with that detailed work. Same shit
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 13 hours
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Re: Cultivation General Discussion [Re: rockyfungus]
#27482844 - 09/26/21 02:06 PM (2 years, 4 months ago) |
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This looks like pan myc to me, what do you think?
-------------------- Willpower is the one true virtue
  
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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yo that was over 24 hours with no post on general wtf
That pic kinda sucks but it looks like pans to me.
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Guerrilla
Bumbaclart


Registered: 01/30/21 
Posts: 3,170
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: sandman420]
#27482854 - 09/26/21 02:17 PM (2 years, 4 months ago) |
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God damn.. I'll add to the conversation. Probably nothing worthy but I like typing on here even if I end up talking to my fucking self.
So I got a bacticinerator last week. I'm in the UK, and the fuckers, one of the "top" lab suppliers sent it with an EU plug. Very illegal. Trading standards would not be happy. Cunts wouldnt even refund me without a return. I'm getting it changed to a UK plug tomorrow by an electrician. There's 2 settings on it, high and low. Anyone with experience got an idea for timing on each setting, say for a scalpel?
-------------------- Being pissed on does not make you a real man.
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: sandman420]
#27482859 - 09/26/21 02:23 PM (2 years, 4 months ago) |
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Quote:
sandman420 said: yo that was over 24 hours with no post on general wtf
That pic kinda sucks but it looks like pans to me.
Lulrite.
I finally got enigma cuz none of the cocksuckers on here is willing to break the law for a stranger ha. Calm down. From a vendor online even which is crazy. I ordered it like 3 weeks ago, and I shit you not like immediately after I ordered the website said the vendor went on vacation, so I immediately thought I got scammed obviously, but thought oh well 20 bucks, shouldn't have done that. Like a week later I get a call from my bank saying there's fraud charges from like another neighbor state, which is wierd cause the vendor is several states away so I wasn't super sure what to think, my bank said they stopped them and I was stoked they did and called me. But anyways, the fucking syringe arrived, LC, which is interesting. Obviously illegal, from Kansas I think, or west Virginia, to Oregon. Ibterestin Anyways lol we will see if it's even enigma ha.
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Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Guerrilla]
#27482874 - 09/26/21 02:30 PM (2 years, 4 months ago) |
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Quote:
Guerrilla said: God damn.. I'll add to the conversation. Probably nothing worthy but I like typing on here even if I end up talking to my fucking self.
So I got a bacticinerator last week. I'm in the UK, and the fuckers, one of the "top" lab suppliers sent it with an EU plug. Very illegal. Trading standards would not be happy. Cunts wouldnt even refund me without a return. I'm getting it changed to a UK plug tomorrow by an electrician. There's 2 settings on it, high and low. Anyone with experience got an idea for timing on each setting, say for a scalpel?
Can you not just a travel adapter?
-------------------- Back once again with the ill behaviour Links I like
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Guerrilla
Bumbaclart


Registered: 01/30/21 
Posts: 3,170
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: Smoothcat]
#27482887 - 09/26/21 02:40 PM (2 years, 4 months ago) |
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Quote:
Smoothcat said:
Quote:
Guerrilla said: God damn.. I'll add to the conversation. Probably nothing worthy but I like typing on here even if I end up talking to my fucking self.
So I got a bacticinerator last week. I'm in the UK, and the fuckers, one of the "top" lab suppliers sent it with an EU plug. Very illegal. Trading standards would not be happy. Cunts wouldnt even refund me without a return. I'm getting it changed to a UK plug tomorrow by an electrician. There's 2 settings on it, high and low. Anyone with experience got an idea for timing on each setting, say for a scalpel?
Can you not just a travel adapter?
Not safe dude. They're not fused, and with the high voltage I'm at a good risk of electrocuting myself or/and burning my house down. They're ok for phone chargers. It's 16A and standard UK plugs only run up to 13A.
Just checked some videos, should just be able to see down the funnel when the blade is red hot. Simple enough.
-------------------- Being pissed on does not make you a real man.
Edited by Guerrilla (09/26/21 02:41 PM)
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Guerrilla]
#27482953 - 09/26/21 03:57 PM (2 years, 4 months ago) |
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You don't have to make it red hot. Just get it hot enough to sizzle the agar.
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: sandman420]
#27482964 - 09/26/21 04:16 PM (2 years, 4 months ago) |
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It's 16 amps?!?!?!
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