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OfflineA.k.a
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Registered: 10/27/19
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27481039 - 09/24/21 07:28 PM (2 months, 6 days ago)

I’ve done a couple moldy plates at the end of a SAB session where I turn it off and do the transfer real quick and it’s been ok.


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OfflineSmoothcatS
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27481129 - 09/24/21 09:13 PM (2 months, 6 days ago)

Not best practice but it can be done and I’ve also done it myself successfully, luck will probably be a factor in how it turns out. I mean if you’re gonna be doing it in SAB anyway when all your shit is away I don’t see why not…how mouldy are we talking here anyway?


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Back once again with the ill behaviour



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OfflineMr. MushieS

Registered: 01/16/20
Posts: 635
Last seen: 18 hours, 57 minutes
Re: Cultivation General Discussion [Re: A.k.a]
    #27481158 - 09/24/21 09:36 PM (2 months, 6 days ago)

Quote:

A.k.a said:
I’ve done a couple moldy plates at the end of a SAB session where I turn it off and do the transfer real quick and it’s been ok.




I think you meant FFU but yeah I've done the same.

Open air transfer at the end of a FFU session with no issues several times.


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OfflineCrackatoaS
Stranger in a strange land
I'm a teapot User Gallery


Registered: 04/01/19
Posts: 4,501
Loc: Over by your Mama's house Flag
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Re: Cultivation General Discussion [Re: Mr. Mushie]
    #27481172 - 09/24/21 09:59 PM (2 months, 6 days ago)

Done the same before and even did one last night.


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Invisiblenatedawgnow
5G free since March 2020
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Registered: 02/09/15
Posts: 8,365
Loc: ation
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Re: Cultivation General Discussion [Re: Crackatoa] * 1
    #27481176 - 09/24/21 10:07 PM (2 months, 6 days ago)

Just start over if the culture keeps contaminating on agar. Have you fruited it?

Unless it's a workhorse culture with top notch potency I say ditch it and
start new rather than fuck with a tammy culture for over a year.


--------------------
Any man's death diminishes me, because I am involved in mankind.
And therefore never send to know for whom the bell tolls; it tolls for thee.




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Offlinerockyfungus
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Registered: 03/01/21
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Re: Cultivation General Discussion [Re: Crackatoa]
    #27481178 - 09/24/21 10:07 PM (2 months, 6 days ago)

You know this type of shit. Trying to tease whatever genetics were in a reddit "sticker". Can't believe people paid for this shit.

That was a month ago so, plates worse now LOL
I'm stubborn and want this damn KSSS to fruit and print.


--------------------
Team frugal


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Invisiblenatedawgnow
5G free since March 2020
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27481183 - 09/24/21 10:21 PM (2 months, 6 days ago)

That looks more like cultivator error. Contam sprinkled all over the
plate like that is pretty tell tale bad technique.


--------------------
Any man's death diminishes me, because I am involved in mankind.
And therefore never send to know for whom the bell tolls; it tolls for thee.




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OfflinePreparationH
apply daily

Registered: 03/28/05
Posts: 17,590
Loc: Amsterdam
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Re: Cultivation General Discussion [Re: natedawgnow]
    #27481198 - 09/24/21 10:51 PM (2 months, 6 days ago)

How long can agar sit solidified in an agar bottle before you decide to make new agar?  I have had some jar sitting with agar for like 2-3 months now, start over?


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OfflineSpaceBabyS
alchemist
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Registered: 11/01/20
Posts: 1,251
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27481240 - 09/24/21 11:39 PM (2 months, 6 days ago)

Quote:

rockyfungus said:
Just started working under a hood. I have some plates I've been working in a SAB for almost a year that just won't stop triching out.

Could I run the hood for a few hours then shut the flow and work in open air to do a transfer?
Or best just to keep going in the SAB?




i run my FFU 24/7. figure it keeps the room cleaner.


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


MUSHBOY'S SHROOM TEA TEK


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InvisiblefahtsterM
Now With 33%More Faht
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Registered: 06/17/06
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27481276 - 09/25/21 12:22 AM (2 months, 6 days ago)

Do HEPA filters eventually get clogged up?


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Offlinerockyfungus
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Re: Cultivation General Discussion [Re: natedawgnow]
    #27481284 - 09/25/21 12:34 AM (2 months, 6 days ago)

Quote:

fahtster said:
Do HEPA filters eventually get clogged up?



They quoted me 7 years with 24/7 use. I'm not really comfortable running it 24/7 as doors are going to open and the AC/heat is going to cycle.

Quote:

natedawgnow said:
That looks more like cultivator error. Contam sprinkled all over the
plate like that is pretty tell tale bad technique.




At first I thought it was operator error. I'm not new to my lab, cultures, or agar. Seems I got bombed between a combination of dirty stickers, summer, and a dirty lab. As I refined my technique and went back to prints I kept contaming. All my clean cultures or known clean prints are still clean.

Some may be my error but when a single print out of 10 keeps showing the same germ...


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Invisiblesandman420
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Registered: 06/17/04
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Re: Cultivation General Discussion [Re: fahtster]
    #27481311 - 09/25/21 01:13 AM (2 months, 6 days ago)

Quote:

fahtster said:
Do HEPA filters eventually get clogged up?




Yes it is called final pressure drop. Typically it is twice the starting pressure.


--------------------
Have no fear of perfection, you'll never reach it.


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: rockyfungus] * 1
    #27481334 - 09/25/21 01:59 AM (2 months, 6 days ago)

Quote:

rockyfungus said:
Quote:

fahtster said:
Do HEPA filters eventually get clogged up?



They quoted me 7 years with 24/7 use. I'm not really comfortable running it 24/7 as doors are going to open and the AC/heat is going to cycle.

Quote:

natedawgnow said:
That looks more like cultivator error. Contam sprinkled all over the
plate like that is pretty tell tale bad technique.




At first I thought it was operator error. I'm not new to my lab, cultures, or agar. Seems I got bombed between a combination of dirty stickers, summer, and a dirty lab. As I refined my technique and went back to prints I kept contaming. All my clean cultures or known clean prints are still clean.

Some may be my error but when a single print out of 10 keeps showing the same germ...



Ya but it's all over the plate, not just where the culture is.

How can it be the culture when contams are growing in spots on the plate that the culture isnt touching?


--------------------
Any man's death diminishes me, because I am involved in mankind.
And therefore never send to know for whom the bell tolls; it tolls for thee.




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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 3,477
Loc: Oregon
Re: Cultivation General Discussion [Re: sandman420]
    #27481433 - 09/25/21 08:00 AM (2 months, 6 days ago)

Quote:

A.k.a said:
I’ve done a couple moldy plates at the end of a SAB session where I turn it off and do the transfer real quick and it’s been ok.





Idk why for any reason you would turn it off. The flow is suppose to blow contams backwards. If you turn it off and then open a contaminated player I'm sure it would spread everywhere.


Quote:

Crackatoa said:
Done the same before and even did one last night.



So now you wait 2 weeks to make sure its clean? Even then contams can be sneeky..


Quote:

sandman420 said:
Quote:

fahtster said:
Do HEPA filters eventually get clogged up?




Yes it is called final pressure drop. Typically it is twice the starting pressure.






So is that when you might worry about contams coming through? Or will they ever? Or will the flow rate just slow, and maybe even filter better because the pore size is becoming smaller as it clogs?


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: QM33]
    #27481438 - 09/25/21 08:15 AM (2 months, 6 days ago)



--------------------
Have no fear of perfection, you'll never reach it.


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Offlinerockyfungus
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Registered: 03/01/21
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Re: Cultivation General Discussion [Re: natedawgnow]
    #27481449 - 09/25/21 08:48 AM (2 months, 6 days ago)

Quote:

natedawgnow said:

How can it be the culture when contams are growing in spots on the plate that the culture isnt touching?




Streaked culture from a syringe. The whole plate was touched by an inoculation loop and most likely contaminated water. I've grown a ton this past year and never had these issues. Any of these reddit "stickers" and I've seen colors I didn't know was possible. I just looked through all these plates that contammed. They all show white with green coming off of it. So the culture is touching the trich on most plates.

If pouring plates in my SAB and letting them sit for months shows no contams is it really my tek?
If swiping those said plates with no spores leads to clean plates is it really my tek?

Not calling you out or saying your wrong and not trying to be defensive. I've fixed my tek in the past and constantly evolved for my environment. I've started exotics and gourmets with no problems at all. Whenever I get contams it's maybe 0.1% and a quick review of the process reveals a mistake.

I've been using the same swab from a trusted member here for over a year with no issues. Not trying to blame reddit or anyone else, just want to figure out if I'm just wasting my time with a shit culture or if it's some crazy small error I'm overlooking.

My wife and myself are both trained in aspetic technique. She's watched over me and has seen no issues. I've had her do the work as she thrives with that detailed work. Same shit


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27482844 - 09/26/21 04:06 PM (2 months, 4 days ago)

This looks like pan myc to me, what do you think?



--------------------
Willpower is the one true virtue



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Invisiblesandman420
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27482846 - 09/26/21 04:09 PM (2 months, 4 days ago)

yo that was over 24 hours with no post on general wtf

That pic kinda sucks but it looks like pans to me.


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Have no fear of perfection, you'll never reach it.


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OnlineGuerrilla
Monanacahuia


Registered: 01/30/21
Posts: 1,066
Loc: United Kingdom Flag
Last seen: 5 minutes, 22 seconds
Re: Cultivation General Discussion [Re: sandman420]
    #27482854 - 09/26/21 04:17 PM (2 months, 4 days ago)

God damn.. I'll add to the conversation. Probably nothing worthy but I like typing on here even if I end up talking to my fucking self.

So I got a bacticinerator last week. I'm in the UK, and the fuckers, one of the "top" lab suppliers sent it with an EU plug. Very illegal. Trading standards would not be happy. Cunts wouldnt even refund me without a return. I'm getting it changed to a UK plug tomorrow by an electrician. There's 2 settings on it, high and low. Anyone with experience got an idea for timing on each setting, say for a scalpel?


--------------------


Yup it’s me again. Shit, I post too much..

fahtster said:
The only bad experience is the one not had.


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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
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Registered: 04/09/20
Posts: 3,477
Loc: Oregon
Re: Cultivation General Discussion [Re: sandman420]
    #27482859 - 09/26/21 04:23 PM (2 months, 4 days ago)

Quote:

sandman420 said:
yo that was over 24 hours with no post on general wtf

That pic kinda sucks but it looks like pans to me.





Lulrite.

I finally got enigma cuz none of the cocksuckers on here is willing to break the law for a stranger ha. Calm down. From a vendor online even which is crazy. I ordered it like 3 weeks ago, and I shit you not like immediately after I ordered the website said the vendor went on vacation, so I immediately thought I got scammed obviously, but thought oh well 20 bucks, shouldn't have done that. Like a week later I get a call from my bank saying there's fraud charges from like another neighbor state, which is wierd cause the vendor is several states away so I wasn't super sure what to think, my bank said they stopped them and I was stoked they did and called me. But anyways, the fucking syringe arrived, LC, which is interesting. Obviously illegal, from Kansas I think, or west Virginia, to Oregon. Ibterestin 
Anyways lol we will see if it's even enigma ha.


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