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Invisiblesandman420
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Registered: 06/17/04
Posts: 4,001
Re: Cultivation General Discussion [Re: mushboy] * 1
    #27479210 - 09/23/21 10:15 AM (4 months, 3 days ago)

#3 from here good read

Quote:

Frequently disinfect gloved hands with 70% ethanol while doing aseptic work.

Although the gloves may initially have been sterile when first worn, they will no doubt have
contacted many nonsterile items while in use.
..




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Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
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Re: Cultivation General Discussion [Re: Camera93]
    #27479225 - 09/23/21 10:28 AM (4 months, 3 days ago)

Quote:

Camera93 said:
no ones doing the hydrogen peroxide followed by vinegar method?



I probably googled for hours trying to find out whether that works, and while I didn't get a clear disconfirmation, my impression is that it's inefficient and makes a really miniscule amount of active ingredient. For reference, when making peracetic acid at home, it needs a mineral acid catalyst (sulfuric acid). And the reaction is slow--max yield occurs after 80-90 hours. (The paper I found only said to wait a day.) This is the paper that describes a multi-day reaction:

https://pubs.acs.org/doi/pdf/10.1021/ja01209a502

In short, you have pretty much no potency if you just spray the two reactants with: 95%+ water, no catalyst, no waiting.


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OfflineOra
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Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27479369 - 09/23/21 01:10 PM (4 months, 3 days ago)

I make a spray called MMS, or sodium chloride. Supposedly it's one of the best disinfectants and kills more than even alcohol in studies.

Anyone ever get darkening on the corners of their substrate? I got these grey/purple looking corners to my substrate now. Not sure if it's mold or not but I just ripped the section off just in case. Should've taken pics, but too late now.


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Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
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Re: Cultivation General Discussion [Re: Ora]
    #27479402 - 09/23/21 01:48 PM (4 months, 3 days ago)

I think you mean chlorine dioxide, made from sodium chlorite. ("MMS" is a marketing term by sleazy pseudoscientists that encourage drinking it.)

But yeah, that's one of the good ones, but I don't recall how fast it acts. Some strong disinfectants are slow (like hydrogen peroxide being very strong but too slow for sanitizing). If it works, I'd keep using it. Though I like the way alcohol dries fast. I use ethanol-based sanitizer so I don't worry about inhaling large amounts.


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OfflineOra
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Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27479404 - 09/23/21 01:50 PM (4 months, 3 days ago)

Oh yeah chlorine dioxide. Sodium chloride is salt haha


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Onlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: Ora]
    #27479742 - 09/23/21 07:15 PM (4 months, 2 days ago)

Washed my beer with soap today because of these posts


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OfflineA.k.a
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27479763 - 09/23/21 07:33 PM (4 months, 2 days ago)

Suds on suds.


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LAGM2020


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: A.k.a] * 1
    #27479826 - 09/23/21 08:26 PM (4 months, 2 days ago)

honestly the beer helmet is the best way to go about it


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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OfflineA.k.a
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Re: Cultivation General Discussion [Re: TedsDead]
    #27479841 - 09/23/21 08:49 PM (4 months, 2 days ago)

Lmao


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OfflineSpaceBabyS
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Re: Cultivation General Discussion [Re: QM33]
    #27479874 - 09/23/21 09:34 PM (4 months, 2 days ago)

my tam rate plummeted since i got the FFU. I try to do all my work now right up near the filter. the SAB was a fucking nightmare.

I left a plate open in the flow for about 12 hours and it's still pristine, tho maybe it dried out too much to show tams :shrug:
Quote:

QM33 said:
Quote:

Quirkmeister92 said:
I actually use a liquid sanitizer. It's 80% alc with hydrogen peroxide and propolyene glycol. It lingers long enough.




That could be it, the h202 or PG leaving residues eve , or the high alc content... I heard 70% is best because of osmosis.
To go along with this sanitation experiment I also testing sanitized steel, sanitized fingers and gloves, but also with 70%vs 91. I couldn't find 99 when I was at the store. I do have some everclear tho




70% disinfects better. i buy 99 and dilute it with distilled h2o
cause it evaporaates slowwwer


--------------------
SpaceBaby
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OfflineBig_DubS
waiting
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27479996 - 09/23/21 11:27 PM (4 months, 2 days ago)

I've also diluted >90% using water before when I couldn't find 70%

I care more about washing my hands at the beginning of the mush work than showering myself in iso.


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: Big_Dub] * 1
    #27480270 - 09/24/21 06:26 AM (4 months, 2 days ago)

I don't mean to sound like an asshole (or sound old school:lol:) but if you cant get a SAB to work I honestly dont recommend an FFU or hood because it will be used as a crutch for proper technique.  even in the shittiest conditions good technique can give you good results.  At the same time people that happen to employ good techniques in a shitty environment and take it for granted thinking its easy and neglect what needs  to  actually  be done in that environment  in order to achieve the right results will not get consistently good results which is the ultimate goal .


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


Edited by TedsDead (09/24/21 06:28 AM)


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Offlinemilkboy
shoeboxer
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Registered: 05/01/21
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Re: Cultivation General Discussion [Re: TedsDead] * 1
    #27480428 - 09/24/21 09:48 AM (4 months, 2 days ago)

Glad you said it, once i figured out my technique my SAB contamination rate went to less then 1%. I think its fucked up people go around saying SAB sucks to noobs.


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SPORES
LAGM 2.022


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OfflineSpaceBabyS
alchemist
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Re: Cultivation General Discussion [Re: TedsDead] * 1
    #27480526 - 09/24/21 11:29 AM (4 months, 2 days ago)

Quote:

TedsDead said:
I don't mean to sound like an asshole (or sound old school:lol:) but if you cant get a SAB to work I honestly dont recommend an FFU or hood because it will be used as a crutch for proper technique.  even in the shittiest conditions good technique can give you good results.  At the same time people that happen to employ good techniques in a shitty environment and take it for granted thinking its easy and neglect what needs  to  actually  be done in that environment  in order to achieve the right results will not get consistently good results which is the ultimate goal .





nah dead, that may very well be legitimate, but my tam rate plummeted since i got the FFU. I try to do all my work now right up near the filter. the SAB was a fucking nightmare.

Different folks may have different issues. Me, I deal with lymphomas cause 9/11, past monster-chemo and ongoing daily oral chemo. And I ain't that young...saw Hendrix and them. So I deal with some tremors, at times more than others, and that impacts work in the cramped environment of [at least my] SAB. I am fucking glad to be rid of it. i note that good sterile technique is quite different from the SAB to the FFU. I had a good run with the SAB but it became ever more physicl impactful

just sayin :shrug:


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK
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OnlineNef
Dangerous Noobness
I'm a teapot User Gallery

Registered: 10/31/18
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27480698 - 09/24/21 01:30 PM (4 months, 2 days ago)

dying to get out of my SAB and into the flow... soon soon I hope

but yes I agree with Ted also

think I saw someone on here say once "doesn't matter the cost of the filter, it won't filter your bad technique"

sums it up pretty good I reckon

the sheer brilliance of the SAB itself is a marvel to behold though in a sense... amazing they work as well as they do for such a simple contraption


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OfflineDERRAYLD
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Registered: 05/13/02
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Re: Cultivation General Discussion [Re: Nef]
    #27480748 - 09/24/21 02:12 PM (4 months, 2 days ago)

Happy as can be today, 10kg of wbs in the pc cooking currently with another 20 kg to go over the next 2 days.


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Offlinerockyfungus
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27480953 - 09/24/21 05:52 PM (4 months, 1 day ago)

Just started working under a hood. I have some plates I've been working in a SAB for almost a year that just won't stop triching out.

Could I run the hood for a few hours then shut the flow and work in open air to do a transfer?
Or best just to keep going in the SAB?


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OfflineA.k.a
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27481039 - 09/24/21 07:28 PM (4 months, 1 day ago)

I’ve done a couple moldy plates at the end of a SAB session where I turn it off and do the transfer real quick and it’s been ok.


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OfflineSmoothcat
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Registered: 10/07/15
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27481129 - 09/24/21 09:13 PM (4 months, 1 day ago)

Not best practice but it can be done and I’ve also done it myself successfully, luck will probably be a factor in how it turns out. I mean if you’re gonna be doing it in SAB anyway when all your shit is away I don’t see why not…how mouldy are we talking here anyway?


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OfflineMr. Mushie

Registered: 01/16/20
Posts: 664
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Re: Cultivation General Discussion [Re: A.k.a]
    #27481158 - 09/24/21 09:36 PM (4 months, 1 day ago)

Quote:

A.k.a said:
I’ve done a couple moldy plates at the end of a SAB session where I turn it off and do the transfer real quick and it’s been ok.




I think you meant FFU but yeah I've done the same.

Open air transfer at the end of a FFU session with no issues several times.


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