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OfflineOra
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Re: Cultivation General Discussion [Re: QM33]
    #27461549 - 09/09/21 11:43 AM (4 months, 13 days ago)

Isn't lc impossible to know until you test on agar? (Besides really obvious things like green growth)

Anyone know why APE get all soft? Is it just ripening/rotting? Does it lose potency or something?


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: Ora]
    #27461555 - 09/09/21 11:47 AM (4 months, 13 days ago)

from the pics ive seen agar lc always looks turbid but I don't run it myself.


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Ora] * 1
    #27461568 - 09/09/21 11:57 AM (4 months, 13 days ago)

Quote:

Ora said:
Isn't lc impossible to know until you test on agar? (Besides really obvious things like green growth)

Anyone know why APE get all soft? Is it just ripening/rotting? Does it lose potency or something?





I wouldn't say it's imposible to know. If you make a clear enough broth (strength ranging from 0,1 to 0,3% LME) the resulting broth should be clear enough all throughout the colony's development in the liquid.
If the broth gets abnormally turbid then you're most likely looking at a contaminated bacterial LC.
The higher the nute contentration the more difficult it gets to detect the presence of bacteria. Likewise, other media other than LME might make the broth more cloudy and with higher amounts of sediment.
Also, most times a mold LC doesn't show green, so mold is definitely much harder to spot than bacteria.


About the APE getting soft. Imo that's the easy thing to say to newbies so they get an approximate idea when to harvest. I would never wait for them to get soft, I harvest right before that happens when they're still firm, you'll quickly develop an eye for it. Getting soft means degrading.


Edited by Josex (09/09/21 12:03 PM)


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OfflineOra
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Re: Cultivation General Discussion [Re: Josex]
    #27461580 - 09/09/21 12:07 PM (4 months, 13 days ago)

I'll harvest earlier now, i always tried to wait to the very last minute just because I think they'll grow a liiiittle bit more, but they probably aren't and I'm just wasting fruits lol


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OfflineDERRAYLD
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Re: Cultivation General Discussion [Re: QM33]
    #27461588 - 09/09/21 12:12 PM (4 months, 13 days ago)

Quote:

QM33 said:
Has anyone adding agar to LC noticed anything about identifying contams?
Like, it makes it hard to see if it's turbid...
Also im wondering if I can expect to see present molds or bacteria growing on the surface if there is a contam?




It's nigh on impossible to do a turbidity comparison unfortunately but if anything is going to grow it will grow well on the surface, the mycelium permeates throughout the jar but never sinks.

As with all things, test on agar first.


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Ora]
    #27461593 - 09/09/21 12:16 PM (4 months, 13 days ago)

If you happen to get an uneven flush, there's no problem letting some early birds get a bit soft until the others catch up and ape degrades slower than most cubes anyway.
A bit of softness is not going to affect much if at all with APE, but it's better if you don't go by that rule and instead develop the eye to know when they're all grown up but still firm right before they start getting soft.


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InvisibleQM33
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27461596 - 09/09/21 12:18 PM (4 months, 13 days ago)

Ya although it seemed kind of messy at first, I do think I like it, it has seemed to colonize faster. I think this has to do with the mycelium not bunching up like it tends to without agar, you stir it and it stays mixed, which is pretty tight. I'm hoping in the next couple days I'll se myc formation on the top that I'll be able to judge.


I'm lazy and impatient... I just put shit straight to grains. I don't usually mind it because it's so easy to tell if it's bad after inoculation. I'll just make more grain, kinda wasteful.

But I haven't been upset with my LC since I started wrapping the wring with cling, almost certain that was my fault for the longest time and it makes perfect since, esspecially if your pouring. I had decent luck while using a ship, but once I started pouring it was like the rain had cleared when I realizedthat.


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InvisibleJosex
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27461603 - 09/09/21 12:25 PM (4 months, 13 days ago)

Quote:

DERRAYLD said:
Quote:

QM33 said:
Has anyone adding agar to LC noticed anything about identifying contams?
Like, it makes it hard to see if it's turbid...
Also im wondering if I can expect to see present molds or bacteria growing on the surface if there is a contam?




It's nigh on impossible to do a turbidity comparison unfortunately but if anything is going to grow it will grow well on the surface, the mycelium permeates throughout the jar but never sinks.

As with all things, test on agar first.




Just a bit of agar (I use 0,125%) will make the LC rather turbid but on the other hand, that's just a little puny con if we put it against all the perks that come from adding agar.

Been doing for almost a year now. Even with the agar added to it, I was able to spot a bacterial LC, it was just too obvious.

If you don't have much experience with LC maybe don't add agar if the turbidity is gonna get you all angsty.
0,1 to 0,3% LC without agar will be make things much easier to spot if that's important to you.


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OfflineDERRAYLD
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Re: Cultivation General Discussion [Re: Josex]
    #27461610 - 09/09/21 12:28 PM (4 months, 13 days ago)

Agreed, you won't have a problem identifying something.
The consistency and turbid nature of the agar lc isn't something to be concerned about.


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OfflineAtmozFear
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Re: Cultivation General Discussion [Re: TedsDead]
    #27461638 - 09/09/21 12:53 PM (4 months, 13 days ago)

Thank you everyone for chiming in on the clone discussion, learned a lot!  :cool:


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OfflineDj_karma_magnet

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Re: Cultivation General Discussion [Re: AtmozFear]
    #27461718 - 09/09/21 01:57 PM (4 months, 13 days ago)

Do I have to leave my bags in the dark? When I add my compost to my spawn, do I have to leave it in the dark until it’s fully colonized?


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: Dj_karma_magnet]
    #27461751 - 09/09/21 02:25 PM (4 months, 13 days ago)

No


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: c10h12n2o]
    #27461763 - 09/09/21 02:34 PM (4 months, 13 days ago)

Quote:

c10h12n2o said:
you gotta have your variables VERY standardized and run everything with lots of redundancy to even be able to tell, its kinda a pain in the ass

id say at least 3 of each thing you are testing, ideally way more

if you have something that performs well, it really isnt worth the effort unless you just enjoy strain hunting

the VAST majority of deviation in grows is more to do with environmental/prep factors than genetics. this is why even when working with the exact same genetics you will see vastly different results from different growers (in terms of fruit morphology, yields, canopies, etc). even when its the same grower subtle differences can make a huge difference. standardizing things to a point where you can get meaningful data on genetics is pretty difficult

i had a pretty in depth post here for anyone interested in my personal methods for strain hunting: https://www.shroomery.org/forums/showflat.php/Number/27421903#27421903





yep, and even if you have organized linear growth it would be very difficult to verify that only 1 strain is present


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OfflineOra
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Re: Cultivation General Discussion [Re: TedsDead]
    #27461792 - 09/09/21 03:07 PM (4 months, 13 days ago)

Gave my sis 100mg of APE steeped in hot water for 30 min. I was aiming for a microdose, but apparently she's tripping now lol.

Either she's a lightweight or these APE stronk, or both :lol:


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OfflineEnjoil
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Re: Cultivation General Discussion [Re: Ora]
    #27461804 - 09/09/21 03:18 PM (4 months, 13 days ago)

The low doses are fun sometimes. Like .3-.5.

You gave her a tenth of a gram?


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OfflineOra
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Re: Cultivation General Discussion [Re: Enjoil]
    #27461809 - 09/09/21 03:22 PM (4 months, 13 days ago)

Yeah, normally .1-.2 are considered microdoses


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Offlinegone-pear-shaped
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Re: Cultivation General Discussion [Re: Ora]
    #27461859 - 09/09/21 04:10 PM (4 months, 13 days ago)

I took 0.69 g wet natalensis thinking it would be a microdose. No such luck--it was enough to subtly effect my vision. It must have been because I ate pins. They were really firm--must have had low water, in addition to potentially having higher concentration of actives than mature fruit. Incautious and dumb.


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OfflineQuirkmeister92
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Re: Cultivation General Discussion [Re: Josex]
    #27461872 - 09/09/21 04:20 PM (4 months, 13 days ago)

Quote:

Josex said:
Quote:

DERRAYLD said:
Quote:

QM33 said:
Has anyone adding agar to LC noticed anything about identifying contams?
Like, it makes it hard to see if it's turbid...
Also im wondering if I can expect to see present molds or bacteria growing on the surface if there is a contam?




It's nigh on impossible to do a turbidity comparison unfortunately but if anything is going to grow it will grow well on the surface, the mycelium permeates throughout the jar but never sinks.

As with all things, test on agar first.




Just a bit of agar (I use 0,125%) will make the LC rather turbid but on the other hand, that's just a little puny con if we put it against all the perks that come from adding agar.

Been doing for almost a year now. Even with the agar added to it, I was able to spot a bacterial LC, it was just too obvious.

If you don't have much experience with LC maybe don't add agar if the turbidity is gonna get you all angsty.
0,1 to 0,3% LC without agar will be make things much easier to spot if that's important to you.



When I get home I'll post my agar LC. There's some growth in the slurry that looks like a little sphere amongst the fluff.


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: Quirkmeister92]
    #27461922 - 09/09/21 04:59 PM (4 months, 13 days ago)

Baggggggggzz

Kill Frodo baggins!

15/24 contams in bags last sab session, syringe tested clean on double agar dishes,

I just can't get the fucking BAGS to work reliably


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27461930 - 09/09/21 05:02 PM (4 months, 13 days ago)

bags in a hood>?


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