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Wiscoregon
Harmonic Surfer


Registered: 11/08/11
Posts: 108
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27446111 - 08/28/21 09:32 AM (2 years, 4 months ago) |
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You're absolutely right about that. Tbh I just got a little over excited. Just happy to see anything clean at all. I did something patently stupid in the beginning and all my plates got bacteria (didn't wrap them, put them in a dirty spot). Went to grain before I noticed. Put that bacterial grain to agar, threw extremely thick ropes probably in part because of the bacteria, decided I needed to save the culture and have done like 4-6 transfers (30-40 plates) since spanning 2 months trying to get it clean again, most of it tomentose (despite transferring the thickest ropes I've ever seen in my limited career). Even my new grain that is finally clean is tomentose rn. I'm not even upset about it.
I've heard antibiotic agar is a thing but just never dug around for it because I already had so many plates made, if anyone has a go to recipe, please send it my way, I would really appreciate it. But I'll obviously use the search function and see what I can turn up.
Edited by Wiscoregon (08/28/21 09:34 AM)
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Enjoil



Registered: 10/29/20
Posts: 2,350
Last seen: 8 hours, 10 minutes
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Re: Cultivation General Discussion [Re: Wiscoregon]
#27446151 - 08/28/21 10:12 AM (2 years, 4 months ago) |
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That bowl is sweet. I got this guy in Amsterdam… his name is Sunny, cuz when you rip him the sun shines in your life just right, then reload and ignite….
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Big_Dub
I'm just some guy



Registered: 01/12/11
Posts: 2,640
Loc: Los Angeles
Last seen: 3 hours, 57 minutes
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Re: Cultivation General Discussion [Re: PrimalSoup] 1
#27446247 - 08/28/21 11:44 AM (2 years, 4 months ago) |
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Quote:
PrimalSoup said:
Quote:
Wiscoregon said: I poured a bunch lazily onto some agar plates too, it's kind of messed up because there's standing liquid on the agar plate on half of it, but it's tilted due to wrap on the plate stack, so the dry areas fluffed up with mycelium, looks super duper clean, already blasting rhizos 36 hours in, the original agar that went into the LC's was pure tomatose. Whatever rigor it was searching for it found. I'm a true believer now.
OK but myc will switch from tomentose to rhyzo and back depending on the substrate, and contams - which might be hard to see... So rhyzo growth isn't necessarily equated with rigor, even though lots of people (as do I) select for it. 

Rhizo growth is just ez to identify.
I like to make nutrient poor agar for early xfers/germ and make the myc work for it
Then I'll bump up nutes for later xfers once I've grabbed clean stuff
I never care about rhizo or tomento
-------------------- split_by_nine said: click me you fuck do the right thing
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Ora
Stranger


Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: Big_Dub]
#27446292 - 08/28/21 12:29 PM (2 years, 4 months ago) |
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Do you have pics of plates with low nutes and high nutes?
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 5 months, 17 days
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Re: Cultivation General Discussion [Re: Ora]
#27446326 - 08/28/21 12:50 PM (2 years, 4 months ago) |
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This isn't what you're asking about, but I accidentally poured plates with too little nutes and saw fan regions where the mycelia wouldn't grow. Multiple strains developed this problem. I could not see contamination even with very close examination. But "low nutes" usually just means a little bit less, and my plates were closer to half the usual amount.
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Ora
Stranger


Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 2 years, 1 month
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Interesting. I just wonder because some t4 plates look less organized than what it was xfered from so I was wondering if it's the nutes in the agar or I didn't do good sterile work.
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Big_Dub
I'm just some guy



Registered: 01/12/11
Posts: 2,640
Loc: Los Angeles
Last seen: 3 hours, 57 minutes
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Quote:
gone-pear-shaped said: This isn't what you're asking about, but I accidentally poured plates with too little nutes and saw fan regions where the mycelia wouldn't grow. Multiple strains developed this problem. I could not see contamination even with very close examination. But "low nutes" usually just means a little bit less, and my plates were closer to half the usual amount.

I have a Malabar coast variety that always looks like this.
-------------------- split_by_nine said: click me you fuck do the right thing
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Quote:
gone-pear-shaped said:

This is not a nutrient issue, this is a mold issue and it's a serious pain in the balls to fix. I suggest that you run Josex's invitro brf pucks to clean.
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 5 months, 17 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27446401 - 08/28/21 01:45 PM (2 years, 4 months ago) |
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Oh, wow. Thanks! They're growing together? Freaky. How did you discover that mold gave that effect? How/when did it manifest as mold, if you recall?
I ended up getting rid of the plate (actually two that showed this trait) because I had others with more normal growth.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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My APE and melmak were both like this, could never get them to grow healthy or produce fruits, it was super frustrating. I eventually got a mak tub to fruit one sickly fruit which I cloned and then was able to work with, but that was pure luck.
When there's deletion zones like that it's all trouble.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 1
#27446464 - 08/28/21 02:42 PM (2 years, 4 months ago) |
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That is also called scalloped edges for description sake, and is a sign of intermeshed mold like penut said.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: sandman420] 1
#27446468 - 08/28/21 02:44 PM (2 years, 4 months ago) |
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Ah, good to know "scalloped edge", fucking pain in the balls edge for sure.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27446479 - 08/28/21 02:55 PM (2 years, 4 months ago) |
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Interestingly enough I most recently had it with some melmak swabs too.
About the same experience as you but even shitter. I had some other melmak tp swabs come through great though.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: sandman420]
#27446481 - 08/28/21 02:57 PM (2 years, 4 months ago) |
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The best part about it was that after all of that trouble it didn't even resemble mak.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 5 hours, 11 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27446538 - 08/28/21 03:45 PM (2 years, 4 months ago) |
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That’s so common.
My first run of them was cool but pretty typical cubes, then I ran a different section of the same plate and got normal melmaks.
Definitely some variety in there.
--------------------
LAGM2020     
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: A.k.a]
#27446564 - 08/28/21 04:05 PM (2 years, 4 months ago) |
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Unfortunately I was dealing with a clone so I didn't have the benefit of going back to ms, so I was stuck.
I could have taken a spore print but I figured that I had a dud.
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Big_Dub
I'm just some guy



Registered: 01/12/11
Posts: 2,640
Loc: Los Angeles
Last seen: 3 hours, 57 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27446628 - 08/28/21 04:45 PM (2 years, 4 months ago) |
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Quote:
p9hu7 said:
Quote:
gone-pear-shaped said:

This is not a nutrient issue, this is a mold issue and it's a serious pain in the balls to fix. I suggest that you run Josex's invitro brf pucks to clean.
I suspected it would be mold but I only ever got it from one particular variety and I never could clean it up.
-------------------- split_by_nine said: click me you fuck do the right thing
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wahhwahh
stupid idiot


Registered: 07/19/21
Posts: 296
Last seen: 1 year, 9 months
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Re: Cultivation General Discussion [Re: Big_Dub]
#27446797 - 08/28/21 07:08 PM (2 years, 4 months ago) |
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Anyone ever use loose coir? I've only used bricks before. Went to grow store to pick some up, forgot to mention bricks, ended up with these when I went to the loading bay. Oops.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: wahhwahh]
#27446802 - 08/28/21 07:11 PM (2 years, 4 months ago) |
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Could probably just weigh out 650g
-------------------- Life’s shit, but I’m loving it
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Big_Dub
I'm just some guy



Registered: 01/12/11
Posts: 2,640
Loc: Los Angeles
Last seen: 3 hours, 57 minutes
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Re: Cultivation General Discussion [Re: Wall.E]
#27446810 - 08/28/21 07:19 PM (2 years, 4 months ago) |
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Yeah it works just fine
-------------------- split_by_nine said: click me you fuck do the right thing
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