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InvisibleGuerrilla
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Registered: 01/30/21
Posts: 3,170
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Re: Cultivation General Discussion [Re: coversall]
    #27438082 - 08/22/21 06:14 AM (2 years, 5 months ago)

Thanks brother, what's the reasoning / benefits? What would be the difference between me doing 300ml and 400ml in a 500ml?


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Being pissed on does not make you a real man.


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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
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Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: Cultivation General Discussion [Re: Guerrilla]
    #27438088 - 08/22/21 06:23 AM (2 years, 5 months ago)

Quote:

Guerrilla said:
Thanks brother, what's the reasoning / benefits? What would be the difference between me doing 300ml and 400ml in a 500ml?




I like smaller broths as I only use them once (they must finish quicker as well?). 100ml is a fair bit of LC ime!

I tend to make 5 100ml LCs at a time, then use the quickest one. I don't mod the lids and just swirl them twice a day. I used a cube LC I made the other day, they took ~two weeks to finish up.


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..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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InvisibleGuerrilla
Bumbaclart

Registered: 01/30/21
Posts: 3,170
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Re: Cultivation General Discussion [Re: coversall]
    #27438096 - 08/22/21 06:36 AM (2 years, 5 months ago)

Good stuff - thanks again. I have a bunch of 250ml jars I'll do the same as you I think. So for unmodded LC lids are you using 2 piece or 1 piece? I have both but prefer to use one piece. Are you just having it a little loose in the PC and tightening it up like you would with agar bottles?


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Being pissed on does not make you a real man.


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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
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Re: Cultivation General Discussion [Re: Guerrilla] * 1
    #27438103 - 08/22/21 06:47 AM (2 years, 5 months ago)

Quote:

Guerrilla said:
Good stuff - thanks again. I have a bunch of 250ml jars I'll do the same as you I think. So for unmodded LC lids are you using 2 piece or 1 piece? I have both but prefer to use one piece. Are you just having it a little loose in the PC and tightening it up like you would with agar bottles?




Yeah 1 piece, I use little media bottles for LC as they are great for when I want to pour rather than use a syringe.



Yeah, keep them loose and foiled in the PC then tighten up when you take them out.



--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27438144 - 08/22/21 07:23 AM (2 years, 5 months ago)

Quote:

rockyfungus said:
Holy fucking shit! Wait I've been inverted for 5+ years and only now it's biting my ass?
Sorry photobomb of the lab. Messy as after I cleaned my green Tupperware's I kind of gave up. But flipping a box is a simple solution.


Close to pulling the trigger on a toughshed and converting it to a clean room with a hood or wall unit (not sure about these need to research). Just wondering how tight those suckers are.

Ran a gourmet tent for awhile without issues in same area. Would just shut it down and wait and proceed as usual.





If you’ve been going that long with no issues it might be something else. But you don’t have to flip the box just take the lid off and put the tub right on the counter.


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LAGM2020


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Offlinerockyfungus
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Registered: 03/01/21
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Re: Cultivation General Discussion [Re: A.k.a]
    #27438188 - 08/22/21 07:57 AM (2 years, 5 months ago)

Yeah, figured as much. Wife watched the process and gets it from the outside and from a surgical point of view. She can't figure it out either.

Figured I could just remove the lid. I may try a trich solution from another forum.

To 1 quart of hot water add:
1 Tablespoon baking soda
1 Tablespoon washing soda (aka sodium carbonate)
2 Tablespoons TSP (trisodium phosphate)

shake, careful of pressure build up, take precautions as it's strong


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27438193 - 08/22/21 08:06 AM (2 years, 5 months ago)

You spray the solution on the walls?


I would try tub on the counter, or switching the lid for a damp towel. That’s supposed to catch all the stuff that falls to the bottom.


Do you use a rack or anything to keep stuff elevated?


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Offlinerockyfungus
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Re: Cultivation General Discussion [Re: A.k.a]
    #27438222 - 08/22/21 08:32 AM (2 years, 5 months ago)

Over the years rotated between a damp towel and racks. Took the rack out this year as I was playing with bags and that's tight in a SAB.

Feel weird linking another site. But if anyone wants to read through this thread.
https://mycotopia.net/topic/107483-diy-mold-control-and-prevention-spray/


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OfflineSpaceBaby
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Registered: 11/01/20
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Re: Cultivation General Discussion [Re: rockyfungus]
    #27438537 - 08/22/21 01:28 PM (2 years, 5 months ago)

First 54-qt Mono :rockon:

Spawned 082221

4 jars MAL clone
noccd 0808
p9's millet TEK
coir sub brought to FC w verm
leveled and compressed w griddle press





I evened out that little blip in the right rear.
Can/should I case tomorrow? (gotta make up new coir)
how deep in inches—if yes?

ANOTHER PIECE OF ADVICE SOUGHT

Found a brand new AA 75x for $750 delivered.
Seller has no ratings

ebay has a money back guarantee

It's a serious (over the line) stretch for me, but I have to up my game for the medicinals for which I already have a distributor.

Nothing else is in stock and they are all upward of $1000 back-ordered.

Opinions sought.
(I know it's my call.)


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27438555 - 08/22/21 01:45 PM (2 years, 5 months ago)

Put a top layer on it at spawning, so definitely make up some more cv.
Dump  in enough so that after leveling/compressing it's 1" thick.


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OfflineHuskies
Boop More Snoots
I'm a teapot


Registered: 03/22/16
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27438592 - 08/22/21 02:19 PM (2 years, 5 months ago)

I only have 4 plates for pastyplates left on me at the moment.

Doing a batch of oat jars in the Presto 23qt in a bit


Can I put them on top for the full 2 hours at 15psi without issue? Don't want to run it again just for a few of them, but I do need them to transfer from my good plates/.


--------------------
I call them Huskies cause you tell them to go "Mush! Mush""


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OfflineOra
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Re: Cultivation General Discussion [Re: Huskies]
    #27438612 - 08/22/21 02:43 PM (2 years, 5 months ago)

Sounds like a bit much to cook agar for. You could always try.


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27438624 - 08/22/21 02:48 PM (2 years, 5 months ago)

ty p9

bucket tek:

minimum pasteurization time 2 hours? i will be okay to case 5-6 hrs after spawn? — gotta cook dinner at SpaceMama's hut.

gonna run 2 bricks in a cooler.

btw...

dropped the hammer on the 75x. gotta research bag teks and 99% sure i am gonna start running reishi for my medicinals biz.

thanks for everything. i am grateful to ask who are so knowledgeable and generous with that knowledge and their time to transmit it :bow2:

which  bactizapper do you use? i figure it'll pay for itself in butane not bought.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27438651 - 08/22/21 03:03 PM (2 years, 5 months ago)

No pasteurization, just pour hot water on it and use whenever it's cool, the amount of time that takes is irrelevant.

https://www.ebay.ca/itm/New-Benchmark-B1000-BactiZapper-Infrared-Micro-Sterilizer-/362623773853?mkcid=16&mkevt=1&_trksid=p2349624.m46890.l6249&mkrid=711-127632-2357-0


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InvisibleGuerrilla
Bumbaclart

Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27438687 - 08/22/21 03:28 PM (2 years, 5 months ago)

Quote:

Blue Helix said:
"No one" you say?  Going from LC to bulk works so well that it's all I do anymore.  I get yields that are out of this world doing it.  Here are some typical examples of what it's done for me:



Those little white ones are Ausi Pan Cyans--blew my head off with those suckers--followed by a picture of some Shiitake.  I should have some Goliath Pan Cyans trays fruiting out early Jan.  All of these in the pictures were done from LC directly to bulk.

Here is how I prepare the bags:

http://www.shroomery.org/forums/showflat.php?Cat=&Board=faq&Number=4905507

And you CAN go from print to LC directly (did with most of mine), but you have to have a clean print or clean spore syringe.  Spores to agar clean up to LC is a safer route.  After all, you only need to do agar that one time, and you can store the LC in vacutainers forever after, maybe rejuvinating the culture with a LC-only run anually if you don't use it often.  I'd only store a culture in the form of spores if you want to store on the order of decades or want to work on developing a superior substrain later via multi-spore culture clean ups.

If you do go from spore to LC, you need to understand that you can easily make an infected LC if the print is dirty.  I was given some infected prints and tried to make LC from them directly awhile back.  I learned the hard way what an infected LC looks like and how it acts.  What's it look like?  Well, I don't know all the flavors, but my little experienced showed me it can look like a super dense LC that is on steriods and develops too fast.

So, what is too fast?  A better question is what is the right speed.  Given about 3 ml of liquid culture inoculum, I now believe both cubensis and Pan Cyan liquid cultures should take between 3 to 4 days to fully develop even given ideal incubation temperatures.  If you inoculated with spores add 2 or 3 days.  If you have a stir marble and stir only daily double the figure.  If you are using room temperatures (73F) add about 50% additional time.




I'd be interested to give this a test but the link doesn't work, anyone tried this successfully?


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Guerrilla] * 1
    #27438690 - 08/22/21 03:30 PM (2 years, 5 months ago)

Yeah it’s pretty common with pans and gourmets.


You just mix everything up first, like for cubes coir and grain, then cook it and hit it with lc.


Sort of like giant pf cakes.


--------------------
LAGM2020


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27438691 - 08/22/21 03:31 PM (2 years, 5 months ago)

thanks again p9

4:1 h2o to coir sounds good?

space


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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Offlinedeadmandave
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27438777 - 08/22/21 05:03 PM (2 years, 5 months ago)

Quote:

SpaceBaby said:

ANOTHER PIECE OF ADVICE SOUGHT

Found a brand new AA 75x for $750 delivered.
Seller has no ratings

ebay has a money back guarantee

It's a serious (over the line) stretch for me, but I have to up my game for the medicinals for which I already have a distributor.

Nothing else is in stock and they are all upward of $1000 back-ordered.

Opinions sought.
(I know it's my call.)




i think 750 is a fair price but at least give this a look:
https://www.labx.com/search?sw=All+American+autoclave


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: deadmandave]
    #27438785 - 08/22/21 05:10 PM (2 years, 5 months ago)

How is shroomery Facebook so far behind the shroomery?



I just went on Facebook for the first time in forever and one of the first things to pop up was an argument about whether or not you need to sterilize coir. :confused:


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: deadmandave]
    #27438790 - 08/22/21 05:12 PM (2 years, 5 months ago)

Quote:

deadmandave said:
Quote:

SpaceBaby said:

ANOTHER PIECE OF ADVICE SOUGHT

Found a brand new AA 75x for $750 delivered.
Seller has no ratings

ebay has a money back guarantee

It's a serious (over the line) stretch for me, but I have to up my game for the medicinals for which I already have a distributor.

Nothing else is in stock and they are all upward of $1000 back-ordered.

Opinions sought.
(I know it's my call.)




i think 750 is a fair price but at least give this a look:
https://www.labx.com/search?sw=All+American+autoclave





If you’re gonna be doing consistent grows of more than a few blocks you might want to just build a steam sterilizer. Probably cheaper and can do as many bags as you build it for.


--------------------
LAGM2020


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