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OfflinePrimalSoup
hyperspatial illuminations
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Registered: 11/17/09
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27432500 - 08/17/21 11:25 PM (2 years, 5 months ago)

:whathesaid:


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 


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Offlineskinnylittleblonde
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Re: Cultivation General Discussion [Re: geat]
    #27432526 - 08/18/21 12:04 AM (2 years, 5 months ago)

Quote:

geat said:
Looks like cubes not pans. What's your substrate? What's your sub/spawn ratio? Are you growing these like pans? Arent their requirements different than cubes? Yeah that middle picture looks like contam. Take all that with a grain. I'm new too.



Quote:

geat said:
Looks like cubes not pans. What's your substrate? What's your sub/spawn ratio? Are you growing these like pans? Arent their requirements different than cubes? Yeah that middle picture looks like contam. Take all that with a grain. I'm new too.



Apologies for confusion….Golden Teachers that I’m growing in a pan/tray :smile:
I think it’s about a 50/50 ratio. Maybe closer to 60 sub/40spawn.
If the center and/or far right is contaminated should I just dump The whole tray?

THANK YOU!!!


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Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
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Re: Cultivation General Discussion [Re: skinnylittleblonde]
    #27432552 - 08/18/21 12:40 AM (2 years, 5 months ago)

I would dump it then bake the metal and bleach the plastic. Pseudomonas is bad news--I think it can be managed by reducing humidity/condensation but it's better to eradicate it.


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Offlineskinnylittleblonde
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Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27432637 - 08/18/21 02:28 AM (2 years, 5 months ago)

Quote:

gone-pear-shaped said:
I would dump it then bake the metal and bleach the plastic. Pseudomonas is bad news--I think it can be managed by reducing humidity/condensation but it's better to eradicate it.



THANKS!
So, am I safe to assume that you agree that 3rd pic reflects what may be pseudomonas?
Also, if you don’t mind, do you think white/green patches were caused by too much moisture?


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Offlinegone-pear-shaped
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Re: Cultivation General Discussion [Re: skinnylittleblonde]
    #27432667 - 08/18/21 03:39 AM (2 years, 5 months ago)

Quote:

skinnylittleblonde said:
So, am I safe to assume that you agree that 3rd pic reflects what may be pseudomonas?
Also, if you don’t mind, do you think white/green patches were caused by too much moisture?



I have a pseudo problem right now and some of my pins look like that. The clearer indications I've seen are: caps get brown spots that later turn green and slimy (particularly if the caps are damp). Some abort caps will become brown/black and get covered in slime. Some fruits will abort that shouldn't (they are a bigger size than the time at which pins normally abort for those conditions/strain).

I can't confirm what causes mold, but people typically say it's usually caused by unhealthy spawn (bacteria?). I can say that a too-wet substrate isn't guaranteed to cause mold. Sometimes it will just cause the fruit to be too small in the first flush.


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
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Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27432670 - 08/18/21 03:47 AM (2 years, 5 months ago)

I'm having some trouble with bags. Or at least I believe it's the bags that're the most likely culprit.

Some of my recent tubs have clearly been unhealthy and nothing makes much sense other than something going south with the bags.


------------------------------------------
Starting from the top:

Agar: Less than 1% contamination on plates poured in the hood. Never see contams from technique during transfers either, so unless it's some super sneaky bacteria it can't be my plates.

Liquid Culture: I don't test my LC's but I make sure to flame the shit out of my scalpel, always use a definitively clean culture and make the transfer quickly and smoothly in the flow.

Jars: I eradicated the mold problems I was having previously in uninoculated jars by switching to metal lids and making sure the SFD dries off properly after the PC cycle and that it doesn't get wet at any point later on as well. I don't see any issues arising from my sterile tek during inoculation with LC, and the jars usually colonize healthily in about 10 days.

Bags: I rinse then soak my WBS (which does contain wheat) for about 8 hours in cold water. Quick drain and then I do the bags the way brownbear does by folding the bag and taping the mouth of the bag after squeezing all the air out. PC for 3.5-4 hours. The tape seal sometimes isn't great because I think I'm using different size bags from the tek that when folded over tightly the mouth of the bag goes all the way to the far edge of the bag instead of laying nice and flat in the middle section of the bag, so the tape has to go on at kind of a 90 degree angle. But even if the tape is a little loose after the PC the bags usually pull enough of a vacuum that it seems unlikely that contams are entering through the top of the bag before inoculation somehow and I always let the PC cool overnight before unloading the bags in front of the hood and then storing them in a tote.

For inoculation I G2G 1 qt to 1 bag. I don't see any issues with my technique during inoculation, my flowhood is maybe a little short (~21") but there's still decent room from the top of the bag to the top of the filter. It's possible that the bottom of the jar is exiting the flow a tiny bit when I'm pouring it into the bag but I don't think this would create enough turbulence to knock things down multiple inches into the mouth of the bag.

The bags colonize well, sometimes the very top grains get a little bit dry but I don't spawn it if the myc hasn't at least grabbed onto it a little. I will note that the mycelium seems to look a little less healthy once the bags are colonized compared to how it looked in the jars but overall they still look pretty good, nothing that I'd expect to mold out mid first flush which has almost never happened to me til just recently.

Substrate: CV hydrated with hot tap water, don't think this is a vector, I don't mix coir and grain buckets.
------------------------------------------


I think I'm having a hard time figuring out if it's bacteria or mold that's causing an issue. It's possible that my spawn is bacterial, but I think it's also possible that mold spores are entering the bag somehow and aren't germinating quickly enough to really be visible since 1qt to 1 bag G2G colonizes relatively quickly but it's causing problems down the line.

If it is bacterial I think I have to try changing my grain prep, possibly to a boiling water soak Fooman style or extending the soak to 24 hours and pouring less LC as well. Last resort would be a different grain but that's not easily accessible for me.

If it is mold then I guess I should try folding my bags differently and maybe raising up my hood a couple inches during G2G.

If it's specifically the bags causing problems and not the jars I think I could test that by only G2Ging some of the jars and then spawning the rest in a mono and comparing how they do versus the monos made with the G2G'd bags.


Any thoughts on this? I think I have a decent idea of how to proceed to nail this down, I partially wrote this to clear things up in my own mind but if anyone thinks I'm thinking of this wrong I'd love to know. Thanks ya'll


--------------------
:chems:


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27432690 - 08/18/21 05:08 AM (2 years, 5 months ago)

Quote:

gone-pear-shaped said:
Quote:

skinnylittleblonde said:
So, am I safe to assume that you agree that 3rd pic reflects what may be pseudomonas?
Also, if you don’t mind, do you think white/green patches were caused by too much moisture?



I have a pseudo problem right now and some of my pins look like that. The clearer indications I've seen are: caps get brown spots that later turn green and slimy (particularly if the caps are damp). Some abort caps will become brown/black and get covered in slime. Some fruits will abort that shouldn't (they are a bigger size than the time at which pins normally abort for those conditions/strain).

I can't confirm what causes mold, but people typically say it's usually caused by unhealthy spawn (bacteria?). I can say that a too-wet substrate isn't guaranteed to cause mold. Sometimes it will just cause the fruit to be too small in the first flush.





That’s trich in your picture. It’s pretty rare to catch it that early when it’s still white. I’m sure by now it’s five times bigger and green.


The shrooms will be fine to eat still.


Just means your spawn was off a little.


--------------------
LAGM2020


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Invisiblesandman420
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Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: A.k.a]
    #27432738 - 08/18/21 06:55 AM (2 years, 5 months ago)

Quote:

Bags: I rinse then soak my WBS (which does contain wheat) for about 8 hours in cold water. Quick drain




Quote:

sometimes the very top grains get a little bit dry




8 hours cold water soak is not enough hydration. I suspect that you are partially sterilizing the grains because they are too dry to be thermally conductive.

Is this a new prep method that you started doing to accommodate the larger quantity of grain needed for bags?

Quote:

my flowhood is maybe a little short (~21") but there's still decent room from the top of the bag to the top of the filter. It's possible that the bottom of the jar is exiting the flow a tiny bit when I'm pouring it into the bag but I don't think this would create enough turbulence to knock things down multiple inches into the mouth of the bag.




According to smoke tests I have seen, large items can create over 10" wide swath of turbulence to each side bringing in eddies of outside air. Highly recommend a 30" tall hood for bags. My workflow:





--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (08/18/21 07:03 AM)


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Offlinerockyfungus
dirty
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Registered: 03/01/21
Posts: 1,062
Loc: Front range
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Re: Cultivation General Discussion [Re: sandman420]
    #27432842 - 08/18/21 08:58 AM (2 years, 5 months ago)

Have I been packing my PC too tight? 2hrs at 15psi



On to prep some whole oats.


Edited by rockyfungus (08/18/21 10:57 AM)


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: Cultivation General Discussion [Re: rockyfungus]
    #27433000 - 08/18/21 11:35 AM (2 years, 5 months ago)

Thanks for the reply sandman.

Yeah I used to do Fooman style in the past but since moving to bags I started doing cold soak only but literally just realized my WBS contained wheat so that's a big blunder on my part.

Gonna fish some of the wheat out and try some hydration tests but do you think 24 hour cold soak will do it or I need hot water?

And if it were only partially sterilized, would there be any visible signs in bags that've been sitting around? My bags look fine even a week or two after uninoculated. 

And re: flowhood. That's a bummer, I've been trying to slant the bags to create a bit more vertical space so I'll take that step a bit more seriously, but do you think raising the flowhood is a bad idea? I could stack it on some books to add a few inches, but in that situation the bottom of the bag would be out of the flow obviously. I'd elevate my sealer in this situation as well.


--------------------
:chems:


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Offlinesmalltalk_canceled
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Registered: 07/13/20
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Re: Cultivation General Discussion [Re: Lenz]
    #27433381 - 08/18/21 04:50 PM (2 years, 5 months ago)

Fuck yeah. Good times in Smalltalk Cityyyyy

After fighting the swabs for months, I finally did it

Owned that swab so hard
Fucking trashed it
Murrrrderedd it

Can't even see me without eyes

Finally got APE going



What to even do now? Fuck agar is so satisfying, I love it.

Why do the tards hate agar I can't understand

So good.


--------------------
Willpower is the one true virtue



Edited by smalltalk_canceled (08/18/21 04:55 PM)


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OfflineAbyssinian
Meow Meow


Registered: 05/20/21
Posts: 135
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27433406 - 08/18/21 05:06 PM (2 years, 5 months ago)

Do yall have a specific dose you always take to test potency or just take however much and then judge from there?


--------------------
I felt.


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: Abyssinian]
    #27433419 - 08/18/21 05:18 PM (2 years, 5 months ago)

1 gram is a good test dose.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: BrownBear]
    #27433433 - 08/18/21 05:36 PM (2 years, 5 months ago)

Yeah a gram is a good test.


I actually started taking .5 sometimes too, but I seem to be way more sensitive to them than most people.

A gram is enough that I’ll be full on tripping when I get a strong culture. I’ve dosed more than enough times now to be able to judge shrooms at lower doses and a lot of the time I’m just curious how they are but don’t want to be seeing patterns for hours.



--------------------
LAGM2020


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: A.k.a] * 1
    #27433448 - 08/18/21 05:56 PM (2 years, 5 months ago)



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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27433647 - 08/18/21 08:56 PM (2 years, 5 months ago)

AA 941 or 1941X folks,

How many bags do y'all cram in there? I'm doing 11-12 4lb bags of wheat.

Do you put jar rings on the bottom and then the trivet on top or do you use the stands that come with the 75x? The stand is shown in the picture below.



I feel without the stand I could get 3 more bags in there.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Mr. Mushie]
    #27433658 - 08/18/21 09:13 PM (2 years, 5 months ago)

I'd personally use that rack just so that I could keep my bags out of the water. With the rack in it will be the same volume as a 75x, I do 9 very comfortably but could probably squeeze in a couple more, so the same as you.

I dont like the idea of submerging bags, so it's not worth the extra few imo, especially if you're running a weight as apposed to a valve. You'll have more water to prevent boiling dry.


Edited by Stipe-n Cap (08/18/21 09:20 PM)


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OfflineSpaceBaby
alchemist
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Registered: 11/01/20
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Re: Cultivation General Discussion [Re: BrownBear]
    #27433706 - 08/18/21 10:05 PM (2 years, 5 months ago)

Quote:

Iambrownbear said:
1 gram is a good test dose.




I don't feel 1 gram. :shrug: I could probably microdose with it. I usually test with around 3.25ish.

But long-term sobriety or not, I have alkie tolerance. :rolleyes: Faddiman had to 2x the dose for his alkie lab subjects.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OfflineBrownBear
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27433716 - 08/18/21 10:11 PM (2 years, 5 months ago)

Quote:

SpaceBaby said:
Quote:

Iambrownbear said:
1 gram is a good test dose.




I don't feel 1 gram. :shrug: I could probably microdose with it. I usually test with around 3.25ish.

But long-term sobriety or not, I have alkie tolerance. :rolleyes: Faddiman had to 2x the dose for his alkie lab subjects.




Interesting. I guess we have vastly different tolerance levels. I microdose .33 grams and still feel something. I have mind blowing trips from 3-5 grams.


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: BrownBear]
    #27433727 - 08/18/21 10:30 PM (2 years, 5 months ago)

Quote:

Iambrownbear said:
Quote:

SpaceBaby said:
Quote:

Iambrownbear said:
1 gram is a good test dose.




I don't feel 1 gram. :shrug: I could probably microdose with it. I usually test with around 3.25ish.

But long-term sobriety or not, I have alkie tolerance. :rolleyes: Faddiman had to 2x the dose for his alkie lab subjects.




Interesting. I guess we have vastly different tolerance levels. I microdose .33 grams and still feel something. I have mind blowing trips from 3-5 grams.




Yeah, everybody's different. I wish it weren't so, but such is life. I think p9 mentioned he trips pretty hard on .33g and can't really microdose. My tolerance came down when I quit a calcium channel blacker [Verapamil for clusterheadaches...didn't work anyway, only shrooms work {and acid}] and SSRIs. Rest of the world sinking anxiously into depression during the pandemic and i got off all psych meds. gotta love shrooms. i credit the little fellas.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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