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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: junk_f00d]
#27430378 - 08/16/21 01:58 PM (2 years, 5 months ago) |
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because syringes are dirty. most noobs use syringes. bacterial/dirty cakes can still produce because the colony is kept whole. better chances.
you break that dirty ass colony up and you have the 'syringe to grains' problem.
but when i was a noob i rolled the dice and spawned my cakes and was rewarded with permanent insanity
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A.k.a
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Re: Cultivation General Discussion [Re: mushboy]
#27430476 - 08/16/21 03:35 PM (2 years, 5 months ago) |
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Yeah I’ve been saying shoeboxes are the easiest starter grow forever.
But he’s right if they’re using syringes brf is way safer. But then if you’re using brf you might as well make a jar with a thin layer of it and use it as a plate, then go to grain 
I got lucky at first too, but it was because I happened to get spores from the one place I now know uses centrifuges to clean the spores.
Probably did 20+ jars of syringe to grain with zero losses.
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junk_f00d


Registered: 12/04/15
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Re: Cultivation General Discussion [Re: mushboy]
#27430531 - 08/16/21 04:30 PM (2 years, 5 months ago) |
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Quote:
mushboy said: because syringes are dirty. most noobs use syringes. bacterial/dirty cakes can still produce because the colony is kept whole. better chances.
you break that dirty ass colony up and you have the 'syringe to grains' problem.
but when i was a noob i rolled the dice and spawned my cakes and was rewarded with permanent insanity
well kinda but coir isn't really food so it shouldn't propagate contams in the same way syringe to grain would right? but I see what you're saying, it's definitely a bigger ask to require that your pf cake hold up to being shredded and recolonize vs just fruit
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smalltalk_canceled
Babnik


Registered: 07/13/20
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Re: Cultivation General Discussion [Re: junk_f00d]
#27430537 - 08/16/21 04:34 PM (2 years, 5 months ago) |
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Having problems with semis not properly drop spores, usually faint or partial print, anyone else encountered this multiple times?
Hoping these will drop something decent to use in trade and giveaway
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (08/16/21 04:49 PM)
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A.k.a
Stranger



Registered: 10/27/19
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Printing anything with small caps is kind of tough.
Try adding a wet paper towel and covering them up. I’ve never messed with semis but with stuff like tamps they usually get too dry before they can drop a decent print so you gotta keep it humid or drip water on the caps or something to keep them going.
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junk_f00d


Registered: 12/04/15
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Re: Cultivation General Discussion [Re: A.k.a]
#27430808 - 08/16/21 07:49 PM (2 years, 5 months ago) |
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Quote:
A.k.a said: Printing anything with small caps is kind of tough.
yeah why not just swab? printing those looks like a PITA
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CocaineBuffet
Stranger



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Re: Cultivation General Discussion [Re: junk_f00d]
#27430820 - 08/16/21 07:58 PM (2 years, 5 months ago) |
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Thats what I do with caps that don't or are too small to make good prints.
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smalltalk_canceled
Babnik


Registered: 07/13/20
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Got 1 single print! But it was a complete one. Into the spore book you go.
-------------------- Willpower is the one true virtue
  
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fungilove
Stranger
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does swab introduce more contam possibility ?
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BrownBear
Warrior-Traveler



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Re: Cultivation General Discussion [Re: fungilove]
#27431574 - 08/17/21 12:11 PM (2 years, 5 months ago) |
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I have had very few contaminations from dabbing the center of the plates with swabs. Dropping the swab itself onto the plate more than likely introduces another contam vector due to the handling of the swab.
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Abyssinian
Meow Meow


Registered: 05/20/21
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Re: Cultivation General Discussion [Re: BrownBear]
#27431999 - 08/17/21 05:29 PM (2 years, 5 months ago) |
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Turns out my syzygy are very light spore droppers, I chopped three caps this morning to print and let the rest of the box go since I didn't have time to harvest. The prints I had going for 12 hours were still faint and in the box all the caps had flattened out and weren't ruined with spores. Printing a couple more now.
I got 350g first flush out of this box compared to the 280g flush from the box I harvested yesterday before the veils tore.
I wasn't planning on cloning these but honestly the light spores and not having to get the timing just right is pretty nice. Will need to do some potency testing before 2nd flush to see if its really a keeper.
-------------------- I felt.
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Failboat
Fuck Up
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Re: Cultivation General Discussion [Re: BrownBear]
#27432030 - 08/17/21 05:49 PM (2 years, 5 months ago) |
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Quote:
Iambrownbear said: I have had very few contaminations from dabbing the center of the plates with swabs. Dropping the swab itself onto the plate more than likely introduces another contam vector due to the handling of the swab.
I definitely get contams at the back end of the swabs I snap off, but the growth from the mega colonies that grow off the relatively large sporeload is often quite choice. Since it's on the other edge of the petri you can transfer early without much hassle from the nasty end of the stick.
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A.k.a
Stranger



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Re: Cultivation General Discussion [Re: Failboat]
#27432046 - 08/17/21 06:04 PM (2 years, 5 months ago) |
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Me too.
The thing I hate is half the time the buried swab is the only thing that germinates. I always streak a couple plates and then bury the swab in another one.
I’m gonna try dipping the wood in iso first next time. The myc that comes off the swab is definitely some good stuff.
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LAGM2020     
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smalltalk_canceled
Babnik


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Re: Cultivation General Discussion [Re: A.k.a] 1
#27432096 - 08/17/21 06:24 PM (2 years, 5 months ago) |
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Quote:
A.k.a said: Me too.
The thing I hate is half the time the buried swab is the only thing that germinates. I always streak a couple plates and then bury the swab in another one.
I’m gonna try dipping the wood in iso first next time. The myc that comes off the swab is definitely some good stuff.
Cause swabs suck
-------------------- Willpower is the one true virtue
  
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BrownBear
Warrior-Traveler



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I think the main issue with swabs is that they dry out too quickly. It is best to use them when they are relatively fresh. The only swabs I have had a hard time getting germination from with a dab or swipe are the ones that have been sitting around for longer than a few months. Prints are definitely better for longer term storage but even those will dry out after a while. The best way to store spores is in a syringe.
Edited by BrownBear (08/17/21 07:08 PM)
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rockyfungus
dirty


Registered: 03/01/21
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Re: Cultivation General Discussion [Re: BrownBear]
#27432295 - 08/17/21 08:18 PM (2 years, 5 months ago) |
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No clue what Faht does with his swabs but the wood itself myc'd out and was clean! Instead of my usual swab/stab, i tried a drop of sterile water on the swab before I streaked. I also tried to use a drop of water onto swab for my inoculation loop.
This tool worked really well at grabbing individual fibers too.
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skinnylittleblonde
Stranger

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Re: Cultivation General Discussion [Re: BrownBear]
#27432300 - 08/17/21 08:21 PM (2 years, 5 months ago) |
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Any advise would be appreciated. I’m definitely a newbie & this is my 1st pan attempt at trying to fruit from a tray. Attaching 3 pics, that show tray from left to right. Left looks pretty good, except for green/white patches shown in center pic & far right looks scary. I’m afraid it’s contaminated with pseudomonas I *think that tray got too dry to begin with (humidifier died) then got too humid(once humidifier was replaced) and that I’m struggling with FAE. ANY INSIGHTS?
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geat
The Last


Registered: 09/13/06
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Looks like cubes not pans. What's your substrate? What's your sub/spawn ratio? Are you growing these like pans? Arent their requirements different than cubes? Yeah that middle picture looks like contam. Take all that with a grain. I'm new too.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Cultivation General Discussion [Re: geat]
#27432435 - 08/17/21 10:11 PM (2 years, 5 months ago) |
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definitely cubes not pans
patches look like mold
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Quote:
skinnylittleblonde said:

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