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OfflineBrownBear
Warrior-Traveler
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Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27420171 - 08/08/21 06:16 PM (2 years, 5 months ago)

I am guessing you are referring to a blenderless LI? Never tried a blenderless LI but I have recently tried blended LI with great results. Probably won't go back to LC now.


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 10 hours
Re: Cultivation General Discussion [Re: BrownBear]
    #27420187 - 08/08/21 06:32 PM (2 years, 5 months ago)

So there's no accepted blender less Tek that doesn't use soft agar?

Guess I'll only make soft agar from now on


--------------------
Willpower is the one true virtue



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Offlinemonkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 24 days
Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27420204 - 08/08/21 06:54 PM (2 years, 5 months ago)

any reason you can't do blended?


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OfflineOra
Stranger
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Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 2 years, 1 month
Re: Cultivation General Discussion [Re: monkey_accident]
    #27420212 - 08/08/21 06:59 PM (2 years, 5 months ago)

If you shake the agar in the jar hard enough it breaks apart to little pieces. Worked good enough when I did it.

Do you guys keep agar plates and SAB stuff away from your fruiting bins to reduce contam possibility?


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Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Cultivation General Discussion [Re: Ora]
    #27420235 - 08/08/21 07:22 PM (2 years, 5 months ago)

could do mag stirrer or diy eberbach  with blender base


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (08/08/21 07:23 PM)


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InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada Flag
Trusted Cultivator
Re: Cultivation General Discussion [Re: sandman420]
    #27420242 - 08/08/21 07:27 PM (2 years, 5 months ago)

Mag stirrer isn't enough agitation. I hate using soft agar because it always falls apart into tiny pieces that refuse to get picked up by my scalpel.

I use a regular agar recipe and shake the living fuck out of the media bottle; as long as you don't use too much water it works just fine. 100ml seems to be sweet.


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: Cultivation General Discussion [Re: filthyknees]
    #27420429 - 08/08/21 11:00 PM (2 years, 5 months ago)

Quote:

filthyknees said:
Verum and eat and both fine. Verums been busy and I think eat was second guessing the site after the hack compermised peoples info.




Just hope that everyone is doing well and enjoying their summers. I think a lot of people feel that way since the hack. The sites been fairly dead or at least significantly less active.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: D3_Myc]
    #27420450 - 08/09/21 12:01 AM (2 years, 5 months ago)

Hackers can sniff my turkey neck  :pipesmoke2:


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Invisiblec10h12n2o
serial dilutor
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Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Cultivation General Discussion [Re: Stipe-n Cap] * 1
    #27420463 - 08/09/21 12:29 AM (2 years, 5 months ago)

Quote:

smalltalk_canceled said:
I'm having some LI liquid inoculate problems.

The mycelium just sticks to the agar, making it too random what syringe draws get a good amount of mycelium in it

My dish collection is mostly pretty hard agar.
:rollingsohard:

I had excellent results with a good plate on super soft agar, but having problems with the hard agar


Any fix? The issue is when a wedge is transferred to sterile water, and not enough of the mycelium ends up dispersed in the water

Should I try scraping myc into a smaller container to ensure higher % mycelium in the water and myc not being stuck, but it's still sticking together?

Only use soft agar from now on?
Chop the plate to bits? (Sounds like contam)

It was so easy with soft agar :shocking:





Quote:

p9hu7 said:
Mag stirrer isn't enough agitation. I hate using soft agar because it always falls apart into tiny pieces that refuse to get picked up by my scalpel.

I use a regular agar recipe and shake the living fuck out of the media bottle; as long as you don't use too much water it works just fine. 100ml seems to be sweet.




this is one of the reasons i so strongly prefer LC to LI

an LC with an injection port plus a Josex style agar/clone poke is about as surefire as an LC can get, really not much that can go wrong. i always do the poke w some sterile water in the syringe, so it is basically using a little micro LI to inoculate an LC.. and it expands into SO much LC so fast on a stir plate

in my experience (which admittedly isnt much with LI to grain), the fully colonized LC is much faster on grain than the moderate amount of myc contained in an LI, so it more than compensates for the 3-7 days it takes the LC to be ready

also if you do the micro josex style LI to LC method, there will be virtually no agar in the LC so you will always get a smooth draw, no matter what kind of agar you started with


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (08/09/21 12:30 AM)


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Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 5 months, 17 days
Re: Cultivation General Discussion [Re: D3_Myc]
    #27420464 - 08/09/21 12:30 AM (2 years, 5 months ago)

If you use cross shaped stir bars on a magnetic stirrer, I think it's probably enough agitation and shear to break up hard agar. I haven't tried it, but these stir bars break up everything else I throw at them. They are diamond shaped, not smooth. They create a huge vortex and some noise from chopping through the water and making bubbles. I'd guess the bar size should be 3 cm for the most shearing, because 4 cm might stir it too well.


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InvisibleAshtray161
SettledNomad
Other


Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: Cultivation General Discussion [Re: weetsie]
    #27420501 - 08/09/21 01:33 AM (2 years, 5 months ago)



Any ideas on why my 2nd flush is so lackluster?
The 1st was the canopy in my sig


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312


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Invisiblec10h12n2o
serial dilutor
 User Gallery


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Cultivation General Discussion [Re: Ashtray161]
    #27420514 - 08/09/21 02:05 AM (2 years, 5 months ago)

yep, surface conditions not great, and not much fruiting surface left after harvesting the canopy

a lot of people dont even run a second flush if the first has a good enough canopy


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: c10h12n2o]
    #27420518 - 08/09/21 02:15 AM (2 years, 5 months ago)

Quote:

Ora said:
damn cancer? idk if youve got it under control, but one of the best things i know for it is Rife machines. If your interested you can do some research on the company spooky2. Ive known of them for like 8 years now and theyre pretty legit.




yeah. lymphoma. can't tell by looking. it's been 11 years and it's all good. mskcc doc is happy. so i am. i'll check though. thanks.

Quote:

Feasoghorm said:
Quote:

SpaceBaby said:
I have probably a 1% loss rate on my agar pours...




Same here. 1 out of 100 poured plates will show a contam within two weeks. But as soon as i open a fucking jar of pristine winter white, the whole operation takes a gorilla shit. My control jars remain clean basically indefinitely. I leave two open plates in my sab for a few days while i do transfers 'n shit and they'll be clean.

Been over every aspect of the entire procedure multiple times in different states of mind/mood and i can find nothing other than i have yet to try using a flowhood.
That's really bitchen you can afford one.

There is a small possibility that i may wind up with one b4 the years end. A few things wud have to align, but there's hope.




drives me round the twist. a friend of mine is HVAC wizard. getting specs on components. plan to go 24x30 99.999 .01 microns since i can't find a 0.1.

i watched p9 and some others working in front of a FH. i really think the problem is trying to maintain technique in a cramped space. i'm hoping the savings in untrashed offsets expense over time. :shrug

Quote:

Feasoghorm said:
Quote:

B H O said:
I don’t do drugs. I’ll have a year sober from everything including weed on Tuesday!



That is a serious accomplishment
:kaneclap:

Mush cult...right!
A good three quarters of my lc pour to grain turned out contaminated (showed metabolites at full colonization). My melmak a2g are about 3/4 colonized. Hope them make it to fruit. Never seen a melmak b4 mine eyes. Poured the last Ape lc and last two melmak LCs last night. Put my pc and sab away.

Was never able to get predominantly clean spawn no matter how much time i put into combing over my sterile procedures. There is nothing left to comb. Doesnt add up.

Oh well. I have 90% of a mycology lab to sell off.




c'mon feas. quitters never win. not on are contams kicking mmy ass, i killed my millet Li pours tonight [not killed it good]
i'm still building a flow hood.
i just this cult and community is fun.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleAshtray161
SettledNomad
Other


Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: Cultivation General Discussion [Re: c10h12n2o]
    #27420529 - 08/09/21 02:32 AM (2 years, 5 months ago)

Quote:

c10h12n2o said:
yep, surface conditions not great, and not much fruiting surface left after harvesting the canopy

a lot of people dont even run a second flush if the first has a good enough canopy




Yea I tried to keep up with it but it was hard to dial in. I didnt know if theyd be able to fruit around the stumps, guess not lol.
Oh well, the first flush was pretty damn good so I cant complain. These few stragglers are some chubby bois.


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312


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Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 5 months, 17 days
Re: Cultivation General Discussion [Re: SpaceBaby]
    #27420539 - 08/09/21 03:14 AM (2 years, 5 months ago)

Quote:

SpaceBaby said:
drives me round the twist. a friend of mine is HVAC wizard. getting specs on components. plan to go 24x30 99.999 .01 microns since i can't find a 0.1.



Your friend can probably confirm that filters are usually rated at 0.3 microns since that's the particle size they are worst at filtering. At lower sizes, a different mechanism of particle capture becomes effective, 0.1 and 0.2 micron particles are captured more readily than 0.3.

I suppose 0.01 is the lower bound of effectiveness, but 0.1 would not be. That wouldn't make sense for HEPA-type filters. So you probably won't find filters with a "0.1" label because it's neither the lower bound nor the size of least efficiency.


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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27420572 - 08/09/21 04:44 AM (2 years, 5 months ago)



I am sick to death of turning clean plates and good grain jars into bacterial orgies. I have probably a 1% loss rate on my agar pours, but lately the clones and transfers are a noightmare. I just want to step it up.
forget this edites out link, cause
FAWKFAWKFAWK

Guaranteed to be a piece of shit. Sold and possibly mfctrd by
those ijits at midwest Contm Kits!

Okay,
Quote:

gone-pear-shaped said:
Quote:

SpaceBaby said:
drives me round the twist. a friend of mine is HVAC wizard. getting specs on components. plan to go 24x30 99.999 .01 microns since i can't find a 0.1.



Your friend can probably confirm that filters are usually rated at 0.3 microns since that's the particle size they are worst at filtering. At lower sizes, a different mechanism of particle capture becomes effective, 0.1 and 0.2 micron particles are captured more readily than 0.3.

I suppose 0.01 is the lower bound of effectiveness, but 0.1 would not be. That wouldn't make sense for HEPA-type filters. So you probably won't find filters with a "0.1" label because it's neither the lower bound nor the size of least efficiency.




thanks. i'll be calling a couple of suppliers later today. i want to get the spec sheet for this: https://iscsales.com/item/24x30x5-7-8-biomax-hepa-filter-99-99-koch-k124306-sbm-99/

i am quite unhappy with the SAB, except for making prints.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



Edited by SpaceBaby (08/09/21 04:46 AM)


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Invisiblepaperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
Re: Cultivation General Discussion [Re: SpaceBaby]
    #27420603 - 08/09/21 06:22 AM (2 years, 5 months ago)

I just put mine together within the last couple of weeks.  I made a small one, just 1' x 2'.  But I built a little sliding bottom shelf for it so that my sawdust can sit well below the hood while keeping the plenum up in the air for inoculation.

I wanted my first hood to be something light and portable.  I want to teach others locally.

Anyway, my point was that flowhoods are awesome.  My contam rate has gone way done and my workflow is way way better.

Trying to inoculate sawdust in a SAB is fucking horrible.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley


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Offlinerockyfungus
dirty
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Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 10 hours
Re: Cultivation General Discussion [Re: paperbackwriter]
    #27420624 - 08/09/21 07:05 AM (2 years, 5 months ago)

Trying to inoculate 5+lb bags of sawdust in a SAB is torture. Especially if your transfer jars are almost as big as the box too.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: rockyfungus]
    #27420659 - 08/09/21 07:53 AM (2 years, 5 months ago)

Fucking sab is the bane of my god damned existence.
If i do wind up with a hood and am able to keep going, i wud get a 2'x 3' soze i cud really stretch out and wack my pud. Nice gentile sterile breeze cooling my nutties... Wud be nice.  I jst ate cold meatloaf for breakfast. It was disgusting.


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OfflineA.k.aM
Stranger
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 5 hours, 11 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: Ashtray161]
    #27420662 - 08/09/21 07:54 AM (2 years, 5 months ago)

Quote:

Ashtray161 said:
Quote:

c10h12n2o said:
yep, surface conditions not great, and not much fruiting surface left after harvesting the canopy

a lot of people dont even run a second flush if the first has a good enough canopy




Yea I tried to keep up with it but it was hard to dial in. I didnt know if theyd be able to fruit around the stumps, guess not lol.
Oh well, the first flush was pretty damn good so I cant complain. These few stragglers are some chubby bois.





Another thing is to track the total weight. If you have an enormous first flush with the usual amount of spawn there’s just not much left in there anyway.


--------------------
LAGM2020


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