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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27411934 - 08/02/21 02:03 PM (2 years, 5 months ago) |
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Quote:
Feasoghorm said: Brownbear! I was having withdrawals god damnit where were ya?. Dnt ever leave me again
I took a week vacation. Typically when I take a week off from work, I am working twice as hard at home. This time I went on a relaxing vacation with the family. It has taken me a week after coming back from vacation to get my mind back in gear. 1 week set me back 2+ weeks.
I ordered a warm weather shiitake, golden oyster and pink oyster lc syringes. I think they might be easier to maintain conditions for fruiting than the king's and beech I have been trying to grow this summer.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: BrownBear]
#27411956 - 08/02/21 02:40 PM (2 years, 5 months ago) |
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Went into town and paid 6bux for 10.5lbs of human grade winter white. Doesn't swell as much as the winter white that i get in the 25lb bag for fucking $38. Nice inoculation points. Gonna take a quick nap, rub one out, then prep some wheat.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27411971 - 08/02/21 02:56 PM (2 years, 5 months ago) |
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Is there a reason you go with human feed over animal feed?
That price tag got my acid reflux actin up
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Eugene Gesuale
Jar-Sniffer



Registered: 04/12/20
Posts: 1,920
Loc: The Basement
Last seen: 9 months, 14 days
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Re: Cultivation General Discussion [Re: A.k.a]
#27411987 - 08/02/21 03:06 PM (2 years, 5 months ago) |
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Quote:
A.k.a said: Syringe to grain would be quicker if it works, sometimes I’ll put spores to grain and plates at the same time. That way you might pull off a decent tub or at least enough to get a quick clone.
Have you ever just scraped spores into a grain jar?
-------------------- Everything in life is a trade-off. All posts made by this account are purely satirical in nature.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: natedawgnow]
#27411990 - 08/02/21 03:08 PM (2 years, 5 months ago) |
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Oh is that pricey? Lol I was excited. I run such small batches i like to use the primo stuff. The most i cud have going in my lab at once is six 66qt tubs and 15 6qt shoeboxes. Actually i thnk i only have enough jars to support the tubs.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 8 hours
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27412025 - 08/02/21 03:32 PM (2 years, 5 months ago) |
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When you Americans say human grade grains or a specific grain what does it include?
Is it that they are without a hull?
-------------------- Willpower is the one true virtue
  
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 51 minutes
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Quote:
Adrenognome said:
Quote:
A.k.a said: Syringe to grain would be quicker if it works, sometimes I’ll put spores to grain and plates at the same time. That way you might pull off a decent tub or at least enough to get a quick clone.
Have you ever just scraped spores into a grain jar?
No but I want to say faht has with his no agar techniques.
I know somebody was doing that fairly recently.
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LAGM2020     
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: A.k.a]
#27412086 - 08/02/21 04:19 PM (2 years, 5 months ago) |
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Meh. I dnt have the best speak. Technically "food grade" is for human consumption. Feed grain wud be for livestock and is generally dirtier.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27412092 - 08/02/21 04:25 PM (2 years, 5 months ago) |
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Quote:
Feasoghorm said: Oh is that pricey? Lol I was excited. I run such small batches i like to use the primo stuff. The most i cud have going in my lab at once is six 66qt tubs and 15 6qt shoeboxes. Actually i thnk i only have enough jars to support the tubs.
I mean feed grade is like 13 bucks for a 50lb bag so ya I think it's pretty pricy at 50c a lb and outrageous at over a dollar a lb.
The only real difference is the extra cleaning steps in human food grade. If you're buying organic stuff then there is a slew of differences but the bacteria load is usually higher with organic cereals
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 8 hours
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Re: Cultivation General Discussion [Re: A.k.a] 1
#27412247 - 08/02/21 06:34 PM (2 years, 5 months ago) |
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Quote:
A.k.a said:
Quote:
Adrenognome said:
Quote:
A.k.a said: Syringe to grain would be quicker if it works, sometimes I’ll put spores to grain and plates at the same time. That way you might pull off a decent tub or at least enough to get a quick clone.
Have you ever just scraped spores into a grain jar?
No but I want to say faht has with his no agar techniques.
I know somebody was doing that fairly recently.
Faht has his surgical bag setup...Mad genius.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: rockyfungus]
#27412304 - 08/02/21 07:27 PM (2 years, 5 months ago) |
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Im not concerned about it.
If i was running my entire batch of 60 jars at a time than i wud most certainly reconsider.
Over my long weekend imma prep as many jars as possible and use the inoculation techniques that have historically given me the least amount of trouble. If a significant amount pull through than i'll buy a flowhood and continue. If not, then i'll sell all my mush cult shit, put the money in crypto, and spend time doing what i love with my other hobbie. Either way im happy.
All Melmak & Ape right now. Gonna be beautiful or a beautiful disaster
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 8 hours, 26 minutes
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27412329 - 08/02/21 07:43 PM (2 years, 5 months ago) |
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If you buy the flow hood you'll have better success rates in theory
I got sloppy or something recently cause my jars have been really hit and miss. Tightened up my filter and just burning through petris, taking no prisoners. Got a few LC broths ready to noc.
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cutie
Stranger

Registered: 12/27/20
Posts: 52
Last seen: 11 months, 2 days
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Re: Cultivation General Discussion [Re: cutie] 1
#27412335 - 08/02/21 07:45 PM (2 years, 5 months ago) |
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Quote:
cutie said: This is my PE 5 days after spawning, I just mixed all the sub and grains with no top layer.

Is a casing layer necessary at some point? This thing has had open holes using the Pasty method the whole time at 75 degrees.
I posted this here several days ago and was pointed in the direction of casing the PE. Since I had two tubs made on the same day with the same liquid culture, I decided to case just one tub and see what happened. The tubs were spawned 7/20, and one was cased on 7/29. This picture was taken right before I cased one tub.

This is what they look like now. There are little blobs around the sides and edges of the uncased tub, and the cased tub has yet to really produce anything, it’s just poking through the top. I’m interested to see what happens next. These pictures were taken today, 8/2.

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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 51 minutes
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Re: Cultivation General Discussion [Re: cutie]
#27412433 - 08/02/21 09:17 PM (2 years, 5 months ago) |
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Those look nice and healthy, should be good.
I’d say that it was too far along to get the full benefit of casing but you never know. That’s a fast PE culture to be knotted up like that at 9 days.
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LAGM2020     
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RedVader
Noob



Registered: 07/02/21
Posts: 7
Loc:
Last seen: 2 years, 2 months
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Re: Cultivation General Discussion [Re: A.k.a]
#27412494 - 08/02/21 10:48 PM (2 years, 5 months ago) |
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So I am in the colonization stage in a monotub of my first ever grow. When do I know to begin fruiting? I don’t think I see pinning yet but I hav wall holes blocked off… it been about 5 days since the spawn and substrate went into the tub. And this is what it look like right now.
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: RedVader]
#27412503 - 08/02/21 10:54 PM (2 years, 5 months ago) |
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Technically, you could put tubs into fruiting conditions from the get go.
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: BrownBear]
#27412574 - 08/03/21 01:01 AM (2 years, 5 months ago) |
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we should breed for spores that have a higher thermal death temp than endospores so we can just put a print into a grain jar and PC it and we'd be good
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: RedVader]
#27412575 - 08/03/21 01:02 AM (2 years, 5 months ago) |
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Quote:
RedVader said: So I am in the colonization stage in a monotub of my first ever grow. When do I know to begin fruiting? I don’t think I see pinning yet but I hav wall holes blocked off… it been about 5 days since the spawn and substrate went into the tub. And this is what it look like right now.

What are you growing on? Is this photo edited (or, taken with a filter applied)?
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 5 months, 17 days
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Re: Cultivation General Discussion [Re: junk_f00d]
#27412594 - 08/03/21 02:09 AM (2 years, 5 months ago) |
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Quote:
junk_f00d said: we should breed for spores that have a higher thermal death temp than endospores so we can just put a print into a grain jar and PC it and we'd be good
What an interesting idea. I doubt it's possible, due to endospores having both a much higher kill temperature and d-value. And the time to produce the next generation of mushroom spores is perhaps three weeks, if you do everything with a laboratory level of speed and competence (programmable autoclave, flow hood, microscope view of petri dishes, perfect LC, sterile inoculation and mixing of cake substrate). You could only get 17 generations per year. And you would probably run into a hard biological limit, for example the material the spore wall is made of might decompose at some temperature, regardless of thickness or other mechanisms of heat protection.
But it's a neat idea. I wonder if it could be done. I bet increasing spore survival by even 5°C would be publishable.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Make spores glow in the dark while you're at it. That'd be sick
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