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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: Cultivation General Discussion [Re: sandman420]
#27391365 - 07/17/21 01:45 PM (2 years, 6 months ago) |
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The propane tanks last a while. I lose track but I’m gonna guess about 15 runs at 2 hours each not counting venting.
They go longer than I expected for sure.
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LAGM2020     
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: sandman420]
#27391406 - 07/17/21 02:16 PM (2 years, 6 months ago) |
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Quote:
sandman420 said: It's been a while for me, many years, I was doing it in bags. I think I was doing around 1.5 hours in these bags (broken up for mixing in tubs)

dound another pic, yes wire ties. I was using some variation of the hippie3 supercake formula, RIP big man.

but I can't remember for sure now. I'm gonna get back into it. They gave this


PF deserves more repek! Put some respek on that name!
Also speaking of names deserving repek, what's up Oven Bag! Good to see ya. True OG one of what 10 people still around? My man.
thanks! as long as I’m breathin. Nice tub(s)! That old school PE?
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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
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Re: Cultivation General Discussion [Re: fahtster] 1
#27391411 - 07/17/21 02:21 PM (2 years, 6 months ago) |
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I have used this set up, it works fine, Bayou Classic burner
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: PreparationH] 1
#27391514 - 07/17/21 03:54 PM (2 years, 6 months ago) |
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Does anyone have experience with fluorescing agents? It would be great to add something like Alamar blue to LC broth to detect the presence of bacteria without having to test it.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27391518 - 07/17/21 03:58 PM (2 years, 6 months ago) |
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Tell me more
Edited by Failboat (07/17/21 03:59 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Failboat]
#27391526 - 07/17/21 04:03 PM (2 years, 6 months ago) |
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I'm not sure if Alamar blue would react to mycelium or not, but there must be some reagents out there that would react to bacteria that we can add to broth.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27391529 - 07/17/21 04:05 PM (2 years, 6 months ago) |
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That’s a cool idea!
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: fahtster]
#27391536 - 07/17/21 04:10 PM (2 years, 6 months ago) |
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ive often thought staining a lc for bacteria would be the ideal way to test it if you had a proper lab and scope and the chemicals needed. But just a drop on a slide not the whole thing.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: sandman420] 1
#27391542 - 07/17/21 04:15 PM (2 years, 6 months ago) |
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The issue with testing is that you risk contamination whenever handling the LC, how sweet would it be if your LC changed colour in the presence of bacteria.
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sandman420
Saint PP



Registered: 06/17/04
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27391556 - 07/17/21 04:32 PM (2 years, 6 months ago) |
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You would test it before you are ready to use it immediately. I've also considered that a problem of testing on agar because what if you messed up the lc by withdrawing it somehow and then that grew in there. But if you used it immediately that would be good.
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A.k.a
Stranger



Registered: 10/27/19
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Re: Cultivation General Discussion [Re: sandman420] 2
#27391558 - 07/17/21 04:35 PM (2 years, 6 months ago) |
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Dude lc that changed color if it got dirty would be revolutionary
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LAGM2020     
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,672
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Re: Cultivation General Discussion [Re: A.k.a]
#27391560 - 07/17/21 04:37 PM (2 years, 6 months ago) |
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Or agar that reacts to mold.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: A.k.a]
#27391563 - 07/17/21 04:41 PM (2 years, 6 months ago) |
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It sorta is a thing if you have a scope and slides and the chemicals. I recall one user that does it.
But I think you have to expose it to like an acid wash or some second chemicals or some shit to really see the color under a scope on a slide that gets stained if I remember from lab class. I remember it was an involved process that used like 2 dyes and an acid wash...
I mean obviously you cant see bacteria with the naked eye and I think the whole thing would just turn blue with that dye added in like that.
Edited by sandman420 (07/17/21 04:42 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: sandman420] 1
#27391565 - 07/17/21 04:44 PM (2 years, 6 months ago) |
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I'm not referring to a stain, I'm talking about something like this:
https://www.bio-rad-antibodies.com/alamarblue-cell-viability-assay-resazurin.html
It changes colour when bacteria or other cells are present in fluids. I think it reacts to fungi as well so it wouldn't work for our purposes. There must be a similar reagent the flouresces with bacteria alone.
"the intensity of fluorescence produced is proportional to the number of living cells respiring."
Pretty dope. Not only does it signal presence but it detects concentration. This would be awesome if it didn't detect mycelium.
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A.k.a
Stranger



Registered: 10/27/19
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27391573 - 07/17/21 04:54 PM (2 years, 6 months ago) |
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Bacteria and mycelium have gotta be different enough that something exists that could work.
The concentration thing is pretty cool too, so you can tell just how bad you botched things
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: A.k.a]
#27391576 - 07/17/21 04:59 PM (2 years, 6 months ago) |
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I'll be searching for it, when I find it there will be a new recipe on the boards.
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Ora
Stranger


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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27391588 - 07/17/21 05:12 PM (2 years, 6 months ago) |
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i used https://www.shroomery.org/forums/showflat.php/Number/23188000 for potato agar recipe and the agar was very thick while pouring. i did simmer it for like 5 min though trying to get everything mixed up but its definitely much more thick than in the video. is it normal to be so thick? if its too thick will it lack moisture for the mycelium to grow?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: A.k.a]
#27391667 - 07/17/21 06:53 PM (2 years, 6 months ago) |
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Quote:
A.k.a said: Dude lc that changed color if it got dirty would be revolutionary
So a may have found the answer, I'm going to message some folks in microbiology to see if this is a viable method.
If it is I'll try to get my hands on this shit to run some trials.
https://www.medicalexpo.com/prod/charles-river-laboratories/product-128356-945400.html
https://www.sciencedirect.com/science/article/abs/pii/S0003267001014957
"ATP-bioluminescence reagents will quickly screen for the absence or presence of bacteria, yeasts, and molds.."
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sandman420
Saint PP



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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27391679 - 07/17/21 07:05 PM (2 years, 6 months ago) |
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That's really cool. I wonder if mushroom mycelium counts as a mold. Keep us updated.
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LtLurker
Lost Sailor



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Re: Cultivation General Discussion [Re: sandman420]
#27391687 - 07/17/21 07:10 PM (2 years, 6 months ago) |
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Quote:
sandman420 said: That's really cool. I wonder if mushroom mycelium counts as a mold. Keep us updated.
That's my big question. But even just a bacteria reacting agar would be some advantage.
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