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OfflineAtmozFear
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Registered: 01/25/19
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Re: Cultivation General Discussion [Re: Nimpo]
    #27388023 - 07/14/21 09:44 PM (2 years, 6 months ago)

LOL!!!  Fun stuff man:congrats:


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InvisibleD3_Myc
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Re: Cultivation General Discussion [Re: Nimpo]
    #27388026 - 07/14/21 09:51 PM (2 years, 6 months ago)

When I was 16 I was walking to the gas station I was about 3 houses away from it when something was calling out to me. Like a mental pull and plea for help. It led me to an amethyst with a cheap wire wrap. I don’t buy into stupid wook bullshit but I’ll swear to this day it was sentient. Changed my life and taught me lots of things.  Years later which was the first time I took shrooms (7g) I lost it in front of a buddies house. It got ran over by a car and I never felt anything from it again. I was completely devastated and even now in my 40’s I feel a deep loss thinking about it. I’d randomly venture into headshops or new age wookery places but I’ve never felt anything again


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OfflineAtmozFear
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Re: Cultivation General Discussion [Re: D3_Myc]
    #27388031 - 07/14/21 09:55 PM (2 years, 6 months ago)

We all struggle, life’s a fight


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OfflineOra
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Re: Cultivation General Discussion [Re: AtmozFear]
    #27388032 - 07/14/21 09:55 PM (2 years, 6 months ago)

Hey guys, I just finished my first agar plates and spore syringe to plates. Holy crap I thought I knew what to do but I kept messing up. But one thing I don't remember ever reading about is about the needle.

After I put a drop on the plate (I know your supposed to put it on the innoc loop but I felt like the drops were so huge it would drop right off my loop causing contam) so I dropped it onto the plate and then spread it with the loop. Then I took both syringe and loop outside to flame sterilize both. But when I flame the needle, there's sputtering of liquid that's still near the tip. Is that OK? I was wondering if I put 1 drop onto a plate, that would be the liquid that got heated and killed the spores so then after the first few times I dropped 3 drops for good measure. I flame sterilize the needle after every plate, is this correct? Should I be worried about cooking those spores that are still in the needle?

And then when I finished I didn't know what to do with the syringe lol. I took the needle off, and put some alcohol on the lid and dried it with paper then capped the syringe. Won't I get contams doing this? And now what do I do with the needle? It's probably still got liquid inside it. Should I boil it before putting it away?

Doing agar was a whole bigger ordeal than I thought, I just hope some plates make it out without contams, I did 8 total, 4 of ape and 4 of gt.


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OfflineAtmozFear
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Re: Cultivation General Discussion [Re: Ora]
    #27388037 - 07/14/21 09:58 PM (2 years, 6 months ago)

U can just bust a nut straight to the agar u don’t need a loop


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OfflineFailboat
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27388041 - 07/14/21 10:02 PM (2 years, 6 months ago)

Quote:

smalltalk_canceled said:
Been growing 2 years now, and I still don't have my own clone culture. Starting to wonder if I should clean my fruits with soap?

Agar pins work, but no matter how I take a fruit, biopsy or just a piece, it's always dirty, usually mold or Bacteria, from tubs that flush 2-3 times., Flush great, and is not showings contam at the time I'm taking

And to a degree that I'm not able to take a meaningful isolation.

I do all the regular prep. Should I start picking tub pins instead?
My problem with this is that you're not seeing the fruit pheno

I even can do bags with LI now in a SAB, establishing clone cultures is at the top of the to do list

6/6 tissue samples from natalensis fruit - all dirty



Wipe the fruit with iso and or hydrogen peroxide to clean the outside before tearing it apart, then carefully dig inner strands of the stipe out and place your plate. Big fruits will be easier than skinny fucks especially exotics.


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OfflineOra
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Re: Cultivation General Discussion [Re: AtmozFear]
    #27388044 - 07/14/21 10:05 PM (2 years, 6 months ago)

Quote:

AtmozFear said:
U can just bust a nut straight to the agar u don’t need a loop



That's basically what ended up happening. I just kept shooting that liquid onto the plates. Just spread it a little after.

But like do you flame the needle after every dish? I'm like deer in the headlights with all the liquid sputtering on the flame. I didn't know that was gonna happen. What do?


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OfflineFailboat
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Re: Cultivation General Discussion [Re: Ora]
    #27388049 - 07/14/21 10:08 PM (2 years, 6 months ago)

I don't use needles, just whip it out and spray.


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: Ora]
    #27388056 - 07/14/21 10:12 PM (2 years, 6 months ago)

Quote:

Ora said:
But like do you flame the needle after every dish? I'm like deer in the headlights with all the liquid sputtering on the flame. I didn't know that was gonna happen. What do?





Flame the needle everytime you touch something with it accidently that isn't sterile.  Otherwise IME once is enough.

Yeah it spurts liquid, kind of cool.  No it won't hurt the spores.


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if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 


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OfflineAtmozFear
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Re: Cultivation General Discussion [Re: Ora]
    #27388060 - 07/14/21 10:16 PM (2 years, 6 months ago)

Quote:

Ora said:
Quote:

AtmozFear said:
U can just bust a nut straight to the agar u don’t need a loop



That's basically what ended up happening. I just kept shooting that liquid onto the plates. Just spread it a little after.

But like do you flame the needle after every dish? I'm like deer in the headlights with all the liquid sputtering on the flame. I didn't know that was gonna happen. What do?



Last time I flamed every other plate and like I just said in another thread, zero contam


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OfflineOra
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Re: Cultivation General Discussion [Re: AtmozFear]
    #27388065 - 07/14/21 10:20 PM (2 years, 6 months ago)

Quote:

PrimalSoup said:
Flame the needle everytime you touch something with it accidently that isn't sterile.  Otherwise IME once is enough.

Yeah it spurts liquid, kind of cool.  No it won't hurt the spores.




Got it, thanks. I thought it was probably safer to keep it in the SAB but I wasn't sure so I flamed it, and since it spurted liquid I didn't even dare get it red hot so I probably risked more contam taking it in and out.

Any idea what to do with the needle? I took it off and capped the syringe, but the needles still have spore liquid. Do I wash it with water or boil it?
Quote:

AtmozFear said:
Last time I flamed every other plate and like I just said in another thread, zero contam



It's nice reassurance I didn't screw up that badly then.

My plates are now wrapped in clingwrap and in individual sandwich bags. Do I just keep them in my SAB till myc growth?


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OfflineAtmozFear
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Re: Cultivation General Discussion [Re: Ora]
    #27388074 - 07/14/21 10:28 PM (2 years, 6 months ago)

Good for you for trying agar… wait til u try parafilm- I still have a hard time not stretching enough or too much that it snaps… must’ve said “motherfucker!!” a million times


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OfflineOra
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Re: Cultivation General Discussion [Re: AtmozFear]
    #27388084 - 07/14/21 10:37 PM (2 years, 6 months ago)

It wasn't as simple as all the teks say. I kept forgetting steps or putting my hand over plates. I also probably killed my lungs a bit since I ozoned the crap out of the room and didn't leave it to vent that long before I went to work. Hopefully my sacrifice wasn't in vain and I get some non moldy plates.


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Invisiblec10h12n2o
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Re: Cultivation General Discussion [Re: Ora] * 1
    #27388099 - 07/14/21 11:00 PM (2 years, 6 months ago)

I like precutting 1 inch strips of parafilm off a 4" wide roll, perfect amount even for big plates and speeds up the workflow on a 4" roll, saves money over 2"


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: Ora]
    #27388104 - 07/14/21 11:02 PM (2 years, 6 months ago)

I leave the needle on and put the cover over it.  Careful not to draw blood by getting it wrong.  Then I just store it like that. The syringe is supposed to be sterile except for the spores so it doesn't hurt it to be stored.  Next time you use it just flame the tip again.


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if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 


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OfflineOra
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Re: Cultivation General Discussion [Re: PrimalSoup]
    #27388118 - 07/14/21 11:27 PM (2 years, 6 months ago)

Quote:

PrimalSoup said:
I leave the needle on and put the cover over it.  Careful not to draw blood by getting it wrong.  Then I just store it like that. The syringe is supposed to be sterile except for the spores so it doesn't hurt it to be stored.  Next time you use it just flame the tip again.




Crap I just took the needles off and put the cap back on. Which I wiped with alcohol and paper, and now I'm realizing probably contaminated it. Can I sterilize the needles in a PC and put it back on the syringe next time? Well the syringe opening may be contaminated already since I wiped the cap before putting it back on.


Edited by Ora (07/14/21 11:30 PM)


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Offlinegone-pear-shaped
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Re: Cultivation General Discussion [Re: Ora]
    #27388136 - 07/15/21 12:22 AM (2 years, 6 months ago)

Quote:

Quirkmeister92 said:
Wipe the fruit with iso and or hydrogen peroxide to clean the outside before tearing it apart, then carefully dig inner strands of the stipe out and place your plate. Big fruits will be easier than skinny fucks especially exotics.



Peroxide is strong but slow. I'd stick with alcohol. Or be a badass and use acidified bleach (aka hypochlorous acid) (dilute in 99 parts water, then adjust pH 5-7, or wing it by adding a quarter as much vinegar as the bleach).

Quote:

Ora said:
It wasn't as simple as all the teks say. I kept forgetting steps or putting my hand over plates. I also probably killed my lungs a bit since I ozoned the crap out of the room and didn't leave it to vent that long before I went to work. Hopefully my sacrifice wasn't in vain and I get some non moldy plates.



This is probably okay for a spore syringe, but be careful with ozone and dry spores. If the concentration is strong enough, it may kill them.

Quote:

Ora said:
Crap I just took the needles off and put the cap back on. Which I wiped with alcohol and paper, and now I'm realizing probably contaminated it. Can I sterilize the needles in a PC and put it back on the syringe next time? Well the syringe opening may be contaminated already since I wiped the cap before putting it back on.



Any reason not to store it in the fridge? It may be slightly contaminated but at least it'll take more time to go bad. (Don't put it near the cold air intake or it may freeze.)


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OfflineGeinstein
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Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27388183 - 07/15/21 01:46 AM (2 years, 6 months ago)

How would you steam pasteurize without a temp gauge?


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: AtmozFear]
    #27388243 - 07/15/21 04:29 AM (2 years, 6 months ago)

Quote:

AtmozFear said:
You’re saying heterosexuality is good right? :eatingout:

And thanks for the clarification :takingnotes:





All sexuality is good. Just don't point that thing at me. :freewilly:


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Offlinerockyfungus
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27388278 - 07/15/21 05:42 AM (2 years, 6 months ago)

I accidentally sat on my crystals am I going to be ok, these vibrations are intense. I think I can feel bodhi-jesus-mohammad-billy mays.

Lost any willpower to keep going with my current projects due to trich not being completely fought. At the point where I may hang the gloves up til fall.


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