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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Feasoghorm] 1
#27381495 - 07/10/21 06:59 AM (2 years, 6 months ago) |
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Anything could be going on with those ships, they're vectors. You could be getting little bits of rubber in your needle, they're sanitized as apposed to sterile so your flame sterilized needle is no longer sterile as it pushes through the rubber, as I'm sure you're aware. You may even be picking up bits of paper towel.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Wall.E]
#27381496 - 07/10/21 06:59 AM (2 years, 6 months ago) |
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he will be soon enough
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Cultivation General Discussion [Re: mushboy]
#27381559 - 07/10/21 08:04 AM (2 years, 6 months ago) |
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Has anyone tried the 3” sterile swabs? I’m sure there’s a collective reason they aren’t more commonly employed out side the shorter length. I’m contemplating giving them a try, be nice for mailing.
Dukal Cotton Tipped Applicators 3 inch Pack of 200 Swabsticks Wood Shaft 100% Cotton tip Sterile swabsticks for Medical Applications Latex-Free Single use https://www.amazon.com/dp/B0842PHP9C/ref=cm_sw_r_cp_api_glt_i_WXHCW77FXWRHMHY4K7P3
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: multifractal]
#27381565 - 07/10/21 08:13 AM (2 years, 6 months ago) |
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Quote:
multifractal said: hey yall. I know this really isnt the place for it but I'm on the tail end of about 9 grams of cubes right now and just wanted to let you know that I really appreciate you guys putting up with me and all. i love you guys and we're all a part of that same, wisecracking and humorous in the face of imminent death, beautiful species. do yourself a favor and put your arms around yourself. tell you that you love you and that you just want whats best for you. remind you that you will always be there for you.
ok, probably gonna get banned for this but that's cool folks. im not here to rant and rave and make a distraction, just a blip in the midst of the usual chat and nothing more.
cya.
You can’t get banned for loving on the community.. that’s appropriate in any thread
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 4 hours, 27 minutes
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Re: Cultivation General Discussion [Re: D3_Myc]
#27381568 - 07/10/21 08:16 AM (2 years, 6 months ago) |
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Quote:
D3monic said: Has anyone tried the 3” sterile swabs? I’m sure there’s a collective reason they aren’t more commonly employed out side the shorter length. I’m contemplating giving them a try, be nice for mailing.
Dukal Cotton Tipped Applicators 3 inch Pack of 200 Swabsticks Wood Shaft 100% Cotton tip Sterile swabsticks for Medical Applications Latex-Free Single use https://www.amazon.com/dp/B0842PHP9C/ref=cm_sw_r_cp_api_glt_i_WXHCW77FXWRHMHY4K7P3
Just got a box yesterday. I don’t see why they’d be a problem, I break or get broken swabs all the time.
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LAGM2020     
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LeafyClark
Dr. Greenthumb



Registered: 02/14/19
Posts: 244
Loc: down yonder
Last seen: 2 years, 5 months
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Re: Cultivation General Discussion [Re: D3_Myc]
#27381576 - 07/10/21 08:23 AM (2 years, 6 months ago) |
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Quote:
D3monic said: Has anyone tried the 3” sterile swabs? I’m sure there’s a collective reason they aren’t more commonly employed out side the shorter length. I’m contemplating giving them a try, be nice for mailing.
Dukal Cotton Tipped Applicators 3 inch Pack of 200 Swabsticks Wood Shaft 100% Cotton tip Sterile swabsticks for Medical Applications Latex-Free Single use https://www.amazon.com/dp/B0842PHP9C/ref=cm_sw_r_cp_api_glt_i_WXHCW77FXWRHMHY4K7P3
i just pressure cook regular bamboo qtips in a half pint jar (like 50 at a time) and swab both sides and throw them in their own little bags, i got a 300 pack at the dollar tree for a dollar. best bang for your buck
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Drboomer
The lord magnificent


Registered: 09/22/19
Posts: 957
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Re: Cultivation General Discussion [Re: LeafyClark] 4
#27381579 - 07/10/21 08:27 AM (2 years, 6 months ago) |
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Ohhhh yeah
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: fahtster]
#27381581 - 07/10/21 08:29 AM (2 years, 6 months ago) |
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Quote:
fahtster said:
Quote:
multifractal said: hey yall. I know this really isnt the place for it but I'm on the tail end of about 9 grams of cubes right now and just wanted to let you know that I really appreciate you guys putting up with me and all. i love you guys and we're all a part of that same, wisecracking and humorous in the face of imminent death, beautiful species. do yourself a favor and put your arms around yourself. tell you that you love you and that you just want whats best for you. remind you that you will always be there for you.
ok, probably gonna get banned for this but that's cool folks. im not here to rant and rave and make a distraction, just a blip in the midst of the usual chat and nothing more.
cya.
You can’t get banned for loving on the community.. that’s appropriate in any thread 
I always feel so gay on the comedown, want to make a post giving thanks and telling people how awesome this medicine is. It's incredible how 24hrs later I'm my usual grumpy-fuck self. Then I tell myself it was just the drugz...
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LeafyClark
Dr. Greenthumb



Registered: 02/14/19
Posts: 244
Loc: down yonder
Last seen: 2 years, 5 months
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Re: Cultivation General Discussion [Re: Drboomer]
#27381582 - 07/10/21 08:29 AM (2 years, 6 months ago) |
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Quote:
Drboomer said:

Ohhhh yeah
you lucky bastard
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Drboomer]
#27381583 - 07/10/21 08:30 AM (2 years, 6 months ago) |
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Quote:
Drboomer said:

Ohhhh yeah
Fuck to the yes brutha.
You lucky bastard.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Josex]
#27381592 - 07/10/21 08:35 AM (2 years, 6 months ago) |
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Cultivation General Discussion [Re: A.k.a]
#27381598 - 07/10/21 08:44 AM (2 years, 6 months ago) |
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Quote:
A.k.a said:
Quote:
D3monic said: Has anyone tried the 3” sterile swabs? I’m sure there’s a collective reason they aren’t more commonly employed out side the shorter length. I’m contemplating giving them a try, be nice for mailing.
Dukal Cotton Tipped Applicators 3 inch Pack of 200 Swabsticks Wood Shaft 100% Cotton tip Sterile swabsticks for Medical Applications Latex-Free Single use https://www.amazon.com/dp/B0842PHP9C/ref=cm_sw_r_cp_api_glt_i_WXHCW77FXWRHMHY4K7P3
Just got a box yesterday. I don’t see why they’d be a problem, I break or get broken swabs all the time.
I’m giving them a shot, seems like they would mail a lot nicer.
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Drboomer
The lord magnificent


Registered: 09/22/19
Posts: 957
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Re: Cultivation General Discussion [Re: D3_Myc]
#27381621 - 07/10/21 09:05 AM (2 years, 6 months ago) |
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I truly am a lucky bastard. This thing is massive, way bigger than I've ever needed. Now I gotta buy a hood and a ton of bags
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Drboomer]
#27381629 - 07/10/21 09:12 AM (2 years, 6 months ago) |
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Besides my hood it was the best cult purchase I've made, then the sealer after that, then my bactizapper.
Congratulations on the upgrade
Edited by Stipe-n Cap (07/10/21 09:20 AM)
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monkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 24 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27381637 - 07/10/21 09:19 AM (2 years, 6 months ago) |
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the bactizappers are sexy just finished my fh build and couldn't be happier doing any type of sterile work is 10x more enjoyable and clean
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0ptiquest
Stranger


Registered: 07/05/20
Posts: 509
Last seen: 8 days, 13 hours
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my bactizapper is arriving home next week 
i bought this from aliexpress someone know if they are good?
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 10 hours
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Re: Cultivation General Discussion [Re: 0ptiquest]
#27381948 - 07/10/21 02:51 PM (2 years, 6 months ago) |
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Quote:
A.k.a said: Generally if the plate is clean after 4-5 days I’m good with it.
But, I hate testing lc. What I eventually settled on was using untested lc to make one or two grain jars and then if those are clean g2g. Plus I know the lc is good for next time.
Same MO for me, plenty times clean on agar and then fucked on 3-4 grain jars later.
2nd woodbag failure, after running bags upon bags, guess it's time to increase my PC to 4 hours. Rain for once and it's fucking me up. I usually go a squeeze above capacity.
Edit: Since tripping 4th and 5th, I can't get motivated and my cult is backing up, here's LM LC that hopefully is now a sterile print.
tupperware etched and bleached after years of cult
 Also agar->wood (sans grain), top is clean cyans, bottom is horribly dirty ovoids (best I could care to clean the print through depression)
Edited by rockyfungus (07/10/21 03:00 PM)
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 5 months, 17 days
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Re: Cultivation General Discussion [Re: 0ptiquest]
#27381960 - 07/10/21 03:01 PM (2 years, 6 months ago) |
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Can I mix two agar recipes?
People seem to have good experiences with MEA, but I want to optimize for leap off time on grains, so I'll add some grain soak water. (I think colonization also gets a slow start because I inoculate with too small a wedge.) But can I average two common recipes to get the following?
- 83 mL grain soak water (actually grain boil water)
- 3-4 g light malt extract
- 8.5 g agar
- water to 500 mL
Edit: This is called GLME by Frank in his recipe showdown, but he doesn't actually give a recipe for it.
And is grain boil water as nutritious as grain soak water? I assume it's basically the same, but if I should use more if it's less potent (if it's used at a higher ratio than 1/3 in a GSW agar recipe).
Edited by gone-pear-shaped (07/10/21 03:13 PM)
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 4 hours, 27 minutes
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Try out all kinds of mixtures and see what happens.
They’ll all work so it won’t be a waste and you might find a recipe that’s perfect.
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LAGM2020     
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27382040 - 07/10/21 03:56 PM (2 years, 6 months ago) |
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Quote:
p9hu7 said: Anything could be going on with those ships, they're vectors. You could be getting little bits of rubber in your needle, they're sanitized as apposed to sterile so your flame sterilized needle is no longer sterile as it pushes through the rubber, as I'm sure you're aware. You may even be picking up bits of paper towel.
Well, balls.
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