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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370786 - 07/01/21 05:48 PM (2 years, 6 months ago) |
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Quote:
p9hu7 said: Yeah man, There's soo many options available for a cultivator that we can pick and choose whatever suits or particular skill level or desire, its pretty awesome.
I cut my plate in half and plop both halves into a 500ml media bottle with 250 ml sterile water inside, shake the shit out of it and then heavily inoculate my jars, or even sometimes bags. No blades or special lids/vessels. No syringes, just a simple pour.
LC is super awesome for stretching out one culture almost infinitely, One plate and some Josex poke action and you have infinite expansion.
I might actually try your method out when I decide to change things up again. Thanks for the idea.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 11 hours
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370788 - 07/01/21 05:53 PM (2 years, 6 months ago) |
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Do some mycelium break off from their colony just from coming into contact with water or is force always required to loosen them free?
-------------------- Willpower is the one true virtue
  
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 4 hours, 34 minutes
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Force is needed, shaken or blended
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: BrownBear]
#27370794 - 07/01/21 06:00 PM (2 years, 6 months ago) |
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Quote:
Iambrownbear said: I might actually try your method out when I decide to change things up again. Thanks for the idea.
I can't take credit, brother, that's all munch's technique.
Also I just realized that I lowered my water to 150ml, 250 was causing the issue that smalltalk was describing. So disregard that ratio
Edited by Stipe-n Cap (07/01/21 06:07 PM)
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370799 - 07/01/21 06:08 PM (2 years, 6 months ago) |
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I hope I am following this conversation correctly, but I think Smalltalk would have better results with Milo or Millet. Corn is hard to get a heavy inoculation on because of the larger kernel size. So you wouldn't see faster colonization and I am pretty sure the nutrition content is different. Corn works as a grain, but tends to be slower to colonize. That being said I have had great flushes off of corn.
I have to put my in here and say the Oyster Blender Slurry Eats made back in the day is still the best way to do LI or Slurry.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 27 minutes
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Re: Cultivation General Discussion [Re: BrownBear]
#27370801 - 07/01/21 06:10 PM (2 years, 6 months ago) |
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Quote:
Iambrownbear said:
Quote:
p9hu7 said: Yeah man, There's soo many options available for a cultivator that we can pick and choose whatever suits or particular skill level or desire, its pretty awesome.
I cut my plate in half and plop both halves into a 500ml media bottle with 250 ml sterile water inside, shake the shit out of it and then heavily inoculate my jars, or even sometimes bags. No blades or special lids/vessels. No syringes, just a simple pour.
LC is super awesome for stretching out one culture almost infinitely, One plate and some Josex poke action and you have infinite expansion.
I might actually try your method out when I decide to change things up again. Thanks for the idea.
So I think I will noc an LC with a small wedge and split the remaining plate between two bottles like that. Gotta go run the ol presto for media bottles.
I have an oster I could mod too...
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Sockadin]
#27370805 - 07/01/21 06:12 PM (2 years, 6 months ago) |
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Quote:
Sockadin said: I hope I am following this conversation correctly, but I think Smalltalk would have better results with Milo or Millet. Corn is hard to get a heavy inoculation on because of the larger kernel size. So you wouldn't see faster colonization and I am pretty sure the nutrition content is different. Corn works as a grain, but tends to be slower to colonize. That being said I have had great flushes off of corn.
I have to put my in here and say the Oyster Blender Slurry Eats made back in the day is still the best way to do LI or Slurry.
Yeah, good call.
Also I've never tried slurry but I bet it's pretty sweet. I would have run it if I had a reliable blender, but I do not : (
Just ignore that 250ml ratio, quark, it's too much water.
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BrownBear
Warrior-Traveler



Registered: 06/05/20
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Re: Cultivation General Discussion [Re: Sockadin] 1
#27370806 - 07/01/21 06:14 PM (2 years, 6 months ago) |
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Slurry is hands down the best inoculant you can make. It is fast to colonize (3-5 days) and a little bit will go a long way. The trick is to make it muddy rather than watery so it sticks to the grain.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 27 minutes
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Re: Cultivation General Discussion [Re: BrownBear]
#27370809 - 07/01/21 06:16 PM (2 years, 6 months ago) |
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250ml is too much for half plate? How bout whole plate? Thats not much liquid for the ol 500ml bottle after all.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 11 hours
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Re: Cultivation General Discussion [Re: Failboat]
#27370816 - 07/01/21 06:21 PM (2 years, 6 months ago) |
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Ofc I'd have better results with millet
-------------------- Willpower is the one true virtue
  
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370821 - 07/01/21 06:26 PM (2 years, 6 months ago) |
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Quote:
p9hu7 said:
Quote:
Sockadin said: I hope I am following this conversation correctly, but I think Smalltalk would have better results with Milo or Millet. Corn is hard to get a heavy inoculation on because of the larger kernel size. So you wouldn't see faster colonization and I am pretty sure the nutrition content is different. Corn works as a grain, but tends to be slower to colonize. That being said I have had great flushes off of corn.
I have to put my in here and say the Oyster Blender Slurry Eats made back in the day is still the best way to do LI or Slurry.
Yeah, good call.
Also I've never tried slurry but I bet it's pretty sweet. I would have run it if I had a reliable blender, but I do not : (
Just ignore that 250ml ratio, quark, it's too much water.
You might be able to combine munch's method with a grain slurry. Fill your jar 1/3 full with millet or other tiny grain, let it colonize, pour in sterile water and shake vigorously. Not quite a slurry but it would be both a liquid inoculant and a solid inoculant in one.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Failboat] 1
#27370823 - 07/01/21 06:27 PM (2 years, 6 months ago) |
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Quote:
Quirkmeister92 said: 250ml is too much for half plate? How bout whole plate? Thats not much liquid for the ol 500ml bottle after all.
So I found that 250 ml to an entire plate made for sparse leap off, it was too dilute. Munch called for like 50 or 60 ml or something, which I thought to be too little. Anywhere between 100-150 was gtg for me. I think I even posted about it in my millet prep thread, in the comments. Colonization was too slow at 250.
Like I mentioned earlier I usually make 10 master jars, that's 15ml per jar if you use the entire thing which is a lot of liquid. I don't even use the entire 150 usually, so it's still plenty.
Edit* Seeing ol smalltalks issue with poor leap off made me remember, had to go back to my notes.
Edited by Stipe-n Cap (07/01/21 06:46 PM)
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370845 - 07/01/21 06:51 PM (2 years, 6 months ago) |
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Quote:
p9hu7 said: I'm not sure what you mean, with 1 plate I can make enough LI to inoculate 10-15 master jars (usually 10), each jar can then be used to inoculate 4 or 5 bags. (Usually 4) That's pretty efficient considering I get 5-7 day colonization of those masters as apposed to ~2 weeks with a2g. The speed is the same as LC but without having to colonize the LC. I used to run way more plates when I was doing a2g to start my masters, which sucked serious balls. Its always nice talking to you too man : )
Ohhh ok. Intriguing 
I really wish i didnt have such issues with bacteria. Every time i g2g i get a strait bacterial explosion. Constantly fighting that shit. I luv LC until it becomes baterial splooge, which in my lab, most certainly will at some point. Unbelievably sneaky shit, bacteria.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27370846 - 07/01/21 06:52 PM (2 years, 6 months ago) |
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Bacteria is a sneaky bitch.
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monkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 24 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370948 - 07/01/21 07:58 PM (2 years, 6 months ago) |
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the slurry sounds pretty interesting from brownbear's description lol - anyone got a tek they would like to share? I haven't broken my LC/LI cherry yet.. kind of embarrassing but I just go g2g most the time from master jars/plates would there be more myc by volume in a LC than an LI, given the LI is only nocced with half a plate or whatever it may be while the LC has grown out much more it would have more points of inoculation?
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370975 - 07/01/21 08:18 PM (2 years, 6 months ago) |
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I usually had no problems with ~250mL water:1 plate:10 jars for LI at least in terms of colonization speed, would usually finish around 7-10 days.
I am switching from to LI to LC though cause the Oster blade lid set up is just so finicky and unreliable for me. I've had times where it pulled a vacuum after PC and would suck in dirty air when I opened it in the SAB or most recently I had two jars spring tiny leaks thru the silicone during blending which just sucks when you dropped a nice ass plate in there 5 mins prior. Maybe I just suck at making these things but I prefer to keep things simple and reliable when it comes to stuff like this.
I'll probably redo it at some point but getting all the silicone off the blade attachment and reattaching the gasket and siliconing the entire apparatus together and so on is just not my jam. Plus I don't need an endless stash of plates to keep making LI. I like that I can have a couple clean plates of each variety in the fridge at all times and then just pull it out take a tiny piece to LC then put it back in the fridge. Unless the cultures suck it's looking like it'll cut down on a lot of plate work.
Would be interested in giving blenderless LI another shot but I never liked having to prepare soft plates in advance just to be able to shake it up, I'd always be ending up with cultures I made on regular agar that I wanted to make LI out of and couldn't. My fault for not preparing properly or being patient and just taking another transfer but I like how flexible, simple, and expandable LC is so far.
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Vinci
I'm You



Registered: 08/26/19
Posts: 629
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I picked up 4 bags of wheat and my first bag of millet the other day. Millet was like $40 for 50 lbs The wheat is like $14. Anyways I get mouse issues if I keep grain bags in my house sometimes so I keep them in my van parked out front. Never had any bear issues (here there be bears) but a few days ago I found my van door wide open with practically no damage. All the bags looked intact but there was some loose millet lightly scattered. Wasn't sure what happened. Anyways, I found the millet bag elsewhere in my yard, totally ripped up and empty. Bear musta had a 50 lb, $40 meal on me. So now I have 2 reasons not to fuck with millet. I wonder why the bear murdered the millet and never touches the wheat.
Quote:
monkey_accident said: the slurry sounds pretty interesting from brownbear's description lol - anyone got a tek they would like to share? I haven't broken my LC/LI cherry yet.. kind of embarrassing but I just go g2g most the time from master jars/plates would there be more myc by volume in a LC than an LI, given the LI is only nocced with half a plate or whatever it may be while the LC has grown out much more it would have more points of inoculation?
Yes, there is more myc in LC than LI, although ime they both colonize around the same speed. Slurry on the other hand is fast as fuck. I've been making slurries with 3 parts canola oil to 1 part water in honor of starbones. He's the canola king. Haven't done a side by side comparison but it definitely munches through a hole grain jar in 2-3 days. Crazy stuff. Here's an eatyu link for your slurry hymen: https://www.shroomery.org/forums/showflat.php/Number/10648149
-------------------- Vinci loves you
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Speaking of bacteria, 100% of my RW jars have metabolites. Sneaky Motherfucker! I tested that lc on 3 separate plates and nothing.
Well, that LC was made from the first LC i fucked with, and that jar had a rough time. So hopefully my Melmak jars are solid.
This is really pissing me off. There is only 2 times in the life of a spawn jar in my lab where contamination cud enter. Either 2hrs @ 17psi is not sufficiently sterilizing 10qts of perfectly moisture content wheat, or that shit got in my LC.
No doubts about the effectiveness of the ships and sfd's. Guess my confusion is cuz I had these issues with a2g as well. Like 80% of the time there is at least one tiny metabolite in each jar. Damn..all i wanna do is melmak and ape on the smallest level and bacteria is jst dragging me over the coals.
Alright josex poke. When i go to make my Ape LC's i'll try to use the smallest bit of myc to inoculate them. I'll pull out all the sterile technique precautions like i always do. If the Melmak and the Ape come up bacterial and there are no reasonable theories, i will be stuck and mush cult will jst be draining me of capital i cud put into bitcoin or spend on hookers.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Lenz]
#27370989 - 07/01/21 08:37 PM (2 years, 6 months ago) |
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I think 250ml to a blended plate is different than a hand shaken 250 to a plate due to the effectiveness of the blade to blend the agar puck and suspend the myc.
Blenderless leaves relatively large chunks by comparison and doesn't suspended the myc in the solution as efficiently. Plus I didn't run soft agar, I just use the regular recipe.
I had leap off, it worked, but it was a bit wet, sloppy and slow. If I had a blade I'd use it but unfortunately I do not atm.
Limiting the amount of water helped with the myc concentration and gave me faster colonization.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370993 - 07/01/21 08:41 PM (2 years, 6 months ago) |
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Very true, glossed right over the fact that you're doing blenderless, makes sense that's a lotta water to try and bash up a plate effectively in
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