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OfflineSpaceBaby
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I'm a teapot User Gallery


Registered: 11/01/20
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Re: Cultivation General Discussion [Re: sandman420]
    #27370412 - 07/01/21 12:33 PM (2 years, 6 months ago)

Quote:

p9hu7 said:
I have a millet prep deal in my signature. .




And, duh, I've read your sigfile enough times. Well, seen it. Thanks p9. :bow2:
Quote:

sandman420 said:
I have great success with no-prep millet spawn bags

5lb recipe: 3.1 lb millet + 1.9 lb water mix and let sit for 12 hours or so to expand some. Mix again and PC. There is some clumping but it mixes up good and works great. Too bad millet has gone ridiculously expensive here, nearly a dollar a lb at the feed store since last few months.




Thanks. btw, you know the late musician Mark Sandman? Rockin' tuba, fpr realz.

I'm still doing jars.

cheers


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OfflineSpaceBaby
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Registered: 11/01/20
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Re: Cultivation General Discussion [Re: Josex]
    #27370518 - 07/01/21 02:12 PM (2 years, 6 months ago)

Quote:

Josex said:
Quote:

p9hu7 said:
I have a millet prep deal in my signature. .



:whathesaid:

That's the way I've always done it, although must admit I'm curious about flash preps with millet. Crackatoa and cocaine buffet have made teks on it iirc, keep telling myself I should try that shit, but then again  millet is ridiculously expensive where I am.

Also what sandman said.





you do? which?


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OfflineRunningboar026
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Registered: 03/13/20
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Re: Cultivation General Discussion [Re: PreparationH]
    #27370545 - 07/01/21 02:34 PM (2 years, 6 months ago)

those look like some really solid plates there man. good job


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27370564 - 07/01/21 02:49 PM (2 years, 6 months ago)

Quote:

p9hu7 said:
I'm an impatient son of a gun so I prefer LI over LC.




What is the difference? Are they not the same thing? Or are you talking about that slurry shit? I dnt really knw what that is either. Something to do with BRF.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27370568 - 07/01/21 02:52 PM (2 years, 6 months ago)

LI is mycelium suspended in sterile inert water, either by blending or agitation. No growth takes place and is ready to use immediately.

LC is a nutritional broth meant for the growth and expansion of mycelium within the broth which takes time to colonize.


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Offlineskinnylittleblonde
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Re: Cultivation General Discussion [Re: A.k.a]
    #27370581 - 07/01/21 02:59 PM (2 years, 6 months ago)

STRANGER your signature pics are amazing & a source of inspiration


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OfflineFailboat
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Re: Cultivation General Discussion [Re: skinnylittleblonde]
    #27370628 - 07/01/21 03:49 PM (2 years, 6 months ago)

I saw Brownbear mention tossing agar into nags. I might do a few rounds like this to speed up a few cultures I wanna fruit. APEU and Nats.

Got some rare pepper seeds today too, spicy :crazy2:


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: skinnylittleblonde]
    #27370645 - 07/01/21 04:00 PM (2 years, 6 months ago)

Oh. I must have researched it back when i was borderline retarded, cuz i jst looked it up and there it was lol

You must go through a metric shit ton of plates. I despised all that plate work cuz the sab frustrates me. It was like buttsex with a Brazilian ass-model when i learned i cud wait a week for a plate to colonize to dump it into one jar, or wait a week for an LC and impregnate the entire northern hemisphere. I mean, both transfers are done basically at the same time.

Variety is the spice of life. Everyone has their wayz. I like chatting with you, p9.


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OfflinePreparationH
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27370660 - 07/01/21 04:10 PM (2 years, 6 months ago)

Yea one of you guys were telling me to make LI instead of just transferring in 8 wedges into my 8 jars and I was wimping out because I didnt wanna take the time to run my pressure cooker for water sterilization for 30 minutes.  Now I am watching these jars go at typical agar transfer speed.  I could probably make more jars and do LI and they would be done before these actually. 




I still think I made the right choice to avoid any extra contamination vectors :snub:


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: PreparationH] * 1
    #27370663 - 07/01/21 04:12 PM (2 years, 6 months ago)

Agreed
:nodofunderstanding:


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Offlinegabbk
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Re: Cultivation General Discussion [Re: PreparationH]
    #27370675 - 07/01/21 04:23 PM (2 years, 6 months ago)

Quote:

PreparationH said:
Yea one of you guys were telling me to make LI instead of just transferring in 8 wedges into my 8 jars and I was wimping out because I didnt wanna take the time to run my pressure cooker for water sterilization for 30 minutes.  Now I am watching these jars go at typical agar transfer speed.  I could probably make more jars and do LI and they would be done before these actually. 




I still think I made the right choice to avoid any extra contamination vectors :snub:



Interesting, so you'd just PC a syringe with water and then suck the myc up from the agar?


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27370695 - 07/01/21 04:36 PM (2 years, 6 months ago)

Quote:

Feasoghorm said:
Oh. I must have researched it back when i was borderline retarded, cuz i jst looked it up and there it was lol

You must go through a metric shit ton of plates. I despised all that plate work cuz the sab frustrates me. It was like buttsex with a Brazilian ass-model when i learned i cud wait a week for a plate to colonize to dump it into one jar, or wait a week for an LC and impregnate the entire northern hemisphere. I mean, both transfers are done basically at the same time.

Variety is the spice of life. Everyone has their wayz. I like chatting with you, p9.




I'm not sure what you mean, with 1 plate I can make enough LI to inoculate 10-15 master jars (usually 10), each jar can then be used to inoculate 4 or 5 bags. (Usually 4)

That's pretty efficient considering I get 5-7 day colonization of those masters as apposed to ~2 weeks with a2g. The speed is the same as LC but without having to colonize the LC.

I used to run way more plates when I was doing a2g to start my masters, which sucked serious balls.

Its always nice talking to you too man : )


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OfflinePreparationH
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Re: Cultivation General Discussion [Re: gabbk]
    #27370697 - 07/01/21 04:37 PM (2 years, 6 months ago)

No but you're on the right track, I have in the past pressure cooked like 150mL of water in a jar for 30 minutes then cut your colonized agar dish areas in a tic-tac-toe design and drop that into the sterile water.  Mix/swirl it rigorously then just pour that now mycelium laden water into sterile jars of grains.  I always tried to pour enough to wet a majority of the grain but never enough for the liquid to pool at the bottom, if that makes sense. 


Edited by PreparationH (07/01/21 04:45 PM)


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: PreparationH]
    #27370704 - 07/01/21 04:42 PM (2 years, 6 months ago)

Question:


Do LCs grow out faster than a plate? I'm talking growth speed in itself

If not:

Quote:

PreparationH said:
No but you're on the right track, I have in the past pressure cooked like 150mL of water in a jar for 30 minutes then cut your colonized agar dish areas in a tic-tac-toe design and drop that into the sterile water.  Mix/swirl it rigorously then just pour that now mycelium laden water into sterile jars of grains




Should we ditch growing LCs all together?
LI seems superior as there is the same amount of steps, but you are not relying on proper axenic colonization of the nutrient in the LC.

I'm struggling to see any kind of situation where I want to grow out a LC, over adding water to clean agar growth, or adding clean growth to water


--------------------
Willpower is the one true virtue



Edited by smalltalk_canceled (07/01/21 04:45 PM)


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27370727 - 07/01/21 04:59 PM (2 years, 6 months ago)

I like that there's one less step, no colonization of the inoculant.

LI is a quick and efficient/effective way to exponentially increase the surface area of your inoculation source without having to wait for it. Increased speed with one simple trick; )

Mostly a matter of preference though, LC has it's place for sure. LC expands the existing mycelium in a way that LI doesn't. I can make 250ml 150ml or so of Liquid from an entire plate, while 250 ml of LC can be made with a small biopsy.

It depends on what your aim is, and what your preferences are.



Edited by Stipe-n Cap (07/01/21 06:10 PM)


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27370758 - 07/01/21 05:23 PM (2 years, 6 months ago)

I will admit that I still like LC. I also love the speed of slurry. I just get bored with the same old routine. I like to change things up ever so often. It keeps me attentive of my sterile technique tbh.

I would never do what I am planning to do with petri dishes because I cannot just plop out the agar puck and it wouldn't be very cost efficient. I can buy a lot of condiment cups for very cheap. I like the idea of not having to cut wedges, sterilize syringes, blender lids on jars of water. Plain and simple, there are no other steps involved. I just squeeze the puck out of the condiment cup, seal, mix and done.

Besides, I am growing in supplemented bags of substrate now. I am using rgs as spawn and can stretch a 3 qt bag of rgs spawn a long way with those supplemented bags. The rgs is simply used as an inoculant for its high inoculation points and not as a dominant source of nutrition.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: BrownBear] * 1
    #27370765 - 07/01/21 05:32 PM (2 years, 6 months ago)

Yeah man, There's soo many options available for a cultivator that we can pick and choose whatever suits or particular skill level or desire, its pretty awesome.

I cut my plate in half and plop both halves into a 500ml media bottle with 250ml 150ml sterile water inside, shake the shit out of it and then heavily inoculate my jars, or even sometimes bags. No blades or special lids/vessels. No syringes, just a simple pour.

LC is super awesome for stretching out one culture almost infinitely, One plate and some Josex poke action and you have infinite expansion.


Edited by Stipe-n Cap (07/01/21 06:10 PM)


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27370775 - 07/01/21 05:40 PM (2 years, 6 months ago)

See the real lazy researcher named Smalltalk posted some posts about how to do inoculate for bags a page back or two
Had a lot of troubles with bag

Bags the reason I am messing with LI.
Because if you dont have a HOOD, you need a syringe and liquid inoculate



But see what I got for my troubles:

3 growth points

This is one TWC LI from agar wedge.
Where is the massive inoculate points from doing LI? Is like pieces of wedge itself was inoculated. Day 2 or 3 this is.

LI overrated! Natedog false salesman
Wedge was dropped in water, jar shaked, liquid drawn into syringe, flamed and inoculated with 5-6ml each

I'd expect more distrubted colonization, but hey at least this transfer to a 1kg+ corn bag looks clean so far

Moving up from jars only for sure


--------------------
Willpower is the one true virtue



Edited by smalltalk_canceled (07/01/21 05:48 PM)


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27370783 - 07/01/21 05:46 PM (2 years, 6 months ago)

What didn't work was just pouring water on a perfect GT Agar plate, and then shaking not enough to loosen the agar in any way.
(To distribute mycelium)

Then drawing the liquid with a syringe

This ended up not colonizing so far
Seems like you need a cut, or a harder shake?

Glad the traditional "drop wedge in water" worked so well


--------------------
Willpower is the one true virtue



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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27370784 - 07/01/21 05:47 PM (2 years, 6 months ago)

Because you probably have too much water to mycelium, and also because you probably haven't effectively suspended it in solution.

This is what happens before you get it dialed in. I've never used syringes so you may not be getting it down the needle.

Try using less water, use a full plate, and be sure to completely suspend the mycelium in the water while also breaking up the agar bits as much as possible. This isn't an issue with a blender lid but fuck that.

I had this exact problem when starting put with LI.

Also: you don't need a hood, munch has videos showing the process while using a SAB.

https://www.shroomery.org/forums/showflat.php/Number/22833314


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