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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
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Re: Cultivation General Discussion [Re: sandman420]
#27369984 - 07/01/21 06:15 AM (2 years, 6 months ago) |
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Those are hemostats, not needle holders! But I'm sure they can also hold needles.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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That is a needle holder not a hemostat lol you can see the needle groove in the tip. It's for doing stitches and also the professional way to put sterile blades on a scalpel for surgeons.
Edited by sandman420 (07/01/21 06:27 AM)
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A.k.a
Stranger



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Re: Cultivation General Discussion [Re: King87]
#27369997 - 07/01/21 06:31 AM (2 years, 6 months ago) |
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Quote:
King87 said: Sorry for noob question but why is it if you have 2 clean agar plates it's still only 1 plate that you would use for spawn jars? Would putting 2 agar in 1 jar speed up the process?
It will but for some reason not as much as you’d think. One time I put half a plate in a jar and two whole plates in another and they still finished within a day or two of each other.
Makes no sense to me.
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LAGM2020     
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
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Re: Cultivation General Discussion [Re: sandman420]
#27370003 - 07/01/21 06:34 AM (2 years, 6 months ago) |
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Quote:
sandman420 said: That is a needle holder not a hemostat lol you can see the needle groove in the tip. It's for doing stitches and also the professional way to put sterile blades on a scalpel for surgeons.
I stand corrected. I thought it was a common thing rebranded as an specialty thing, like some importers or resellers do.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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I mean it's literally the same thing just with a groove in the tip and shorter jaws no worries I'd never even heard of one before too long ago. Makes it a snap to take blades on and off the handle, highly recommended.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: King87]
#27370013 - 07/01/21 06:46 AM (2 years, 6 months ago) |
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Quote:
King87 said: Sorry for noob question but why is it if you have 2 clean agar plates it's still only 1 plate that you would use for spawn jars? Would putting 2 agar in 1 jar speed up the process?
The only way to significantly gain any speed with agar cultures is to exponentially increase it's surface area, this can be done fastest by blending (or vigorously shaking) it into sterile water and using as liquid inoculant, or by further expanding it via liquid culture, but that takes more time to grow out.
I'm an impatient son of a gun so I prefer LI over LC.
Also: adding more than one plate to a jar can increase the risk of contamination, that's why it's suggested to use a wedge rather than the entire plate; not a big deal for someone with enough confidence in their culture work, but still a potential hazard especially for newer growers.
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SpaceBaby
alchemist



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Re: Cultivation General Discussion [Re: Josex]
#27370050 - 07/01/21 07:35 AM (2 years, 6 months ago) |
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Quote:
Josex said: Nah man, I get what he's saying, tweezers are not the right tool for that and in the past I also had the same issue he's describing.
Damn I need to make a thread on how I germinate swabs because it really gets you good results everytime.
Will also make a side experiment within that thread where I actually swab a fruit, step on the swab and then spit on it and let it dry open air... then see if I can do a grow with said swab. That should be fun to pull off.
re tool...ergo because WTF do I know. I haven't even read a swab tek.
I love the experiment.
I saw the butthurt Psilosopherr gifted you with. Meh. I like the way you wrote the JoPoke TEK, fwiw.
And fuck it, I'll ask here...I am having trouble locating a White Millet TEK. Would that be the same as a WBS TEK, or is there a link for the former someone could drop in here?
-------------------- SpaceBaby SPACEBABY'S LAGM22 THREAD MUSHBOY'S SHROOM TEA TEK Me as a cube
Another Day's Work:
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: SpaceBaby]
#27370051 - 07/01/21 07:36 AM (2 years, 6 months ago) |
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I have a millet prep deal in my signature. .
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370061 - 07/01/21 07:42 AM (2 years, 6 months ago) |
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I have great success with no-prep millet spawn bags
5lb recipe: 3.1 lb millet + 1.9 lb water mix and let sit for 12 hours or so to expand some. Mix again and PC. There is some clumping but it mixes up good and works great. Too bad millet has gone ridiculously expensive here, nearly a dollar a lb at the feed store since last few months.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27370064 - 07/01/21 07:44 AM (2 years, 6 months ago) |
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Quote:
p9hu7 said: I have a millet prep deal in my signature. .

That's the way I've always done it, although must admit I'm curious about flash preps with millet. Crackatoa and cocaine buffet have made teks on it iirc, keep telling myself I should try that shit, but then again millet is ridiculously expensive where I am.
Also what sandman said.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: Lenz] 2
#27370094 - 07/01/21 08:12 AM (2 years, 6 months ago) |
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Quote:
Lenz said: How the hell is one supposed to pull spore laden fibers off of a swab? Everytime I try it with tweezers the cotton is too matted and clumped together so I either don't get anything or I pull the entire top of the swab that has all the spores on it off.
Ive posted this before but if you have two of the same swab set I’ve always thought that if you put a drop of sterile water on one of the tips and then rub the two together, they’ll streak better because you dislodge them. You should still be able to use the second swab later even though you used it to rub against the other. I actually did a test on some white comp paper with a set of PE swabs doing just that and got a pretty noticeable streak:
Here is just rubbing the dry swabs together (obviously where the spores are and you’d do it over one agar plate) and flakes came off:

And here’s the streak after the drop was added and the swabs were again rubbed together.. it’s kinda hard to see but I started at the upper right hand corner and zig zagged to the bottom left hand corner:

I think the biggest thing is dislodging the spores from the swab fibers and rubbing them together definitely achieves that.
Who doesn’t love a good rub down? Obviously burying the tips in agar works but then you’re down a swab tip. Just some food for thought… and if you don’t have two swabs of the same set but have sterile swabs, you could just use a blank one to rub again aar the one you’re trying to streak. Hell, I might get into agar just so I can try this out lol
Faht
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: fahtster]
#27370126 - 07/01/21 08:39 AM (2 years, 6 months ago) |
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You know what I ended up do with some swabs I got last. Well first I tried to cut them with a razor ha,didn't work. But then I was going to try and do a cross with half a swab I had left. So I pretty much just put the swab and a tiny amount, the tiniest, pf the strain I wanted to cross. Like a Sq cm of spores or less ga. Anyway, put the quarte swab and minute spores off foil into a 2 oz jar with about 10cc of water in it. Shook it up, let it sit, shook it, so on while I was working in the labfor like 30 min. Then sucked up the solution with a sterilized syringe. And I've been growing it out. No fruit yet, stay posted for the cross.
But why nit just put a swab in water,shake it,then suck up the spores? Then you have a nice syringe, and you can dilute as you feel necessary, which is super helpful I think for selfing or breeding especially.
Say you put one swab in 10 cc water. You could inoculate 1000 plates probably!
-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum... There are known knowns, there are known unknowns, there are also unknown unknowns. With great privilege comes great responsibility.
  Quantom Qups PROOF AND Soft Drops Turn your Swab to a Syringe and Syringe to Multiple Syringes! No Pours (QuantomStyal)Magic Fruit Leather DMT for IandI
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fahtster
Now With 33%More Faht



Registered: 06/17/06
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Re: Cultivation General Discussion [Re: QM33]
#27370130 - 07/01/21 08:43 AM (2 years, 6 months ago) |
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Haha.. that pretty much exactly what I do.. that’s where the swab rub idea came from. I also have a few crosses I’m working on but I swab the spores on top of each other to the same swab and then rub them together in water to make SS. https://www.shroomery.org/forums/showflat.php/Number/26993868#26993868
Faht
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
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Re: Cultivation General Discussion [Re: fahtster]
#27370136 - 07/01/21 08:45 AM (2 years, 6 months ago) |
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I actually read that while preparing!
Thanks for all your efforts BTW brotha!
-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum... There are known knowns, there are known unknowns, there are also unknown unknowns. With great privilege comes great responsibility.
  Quantom Qups PROOF AND Soft Drops Turn your Swab to a Syringe and Syringe to Multiple Syringes! No Pours (QuantomStyal)Magic Fruit Leather DMT for IandI
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Failboat
Fuck Up
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Re: Cultivation General Discussion [Re: QM33]
#27370146 - 07/01/21 08:52 AM (2 years, 6 months ago) |
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I feel like super swabbin these Avery's and Melmac just to get more height out of Avery's. Harvesting a wall to wall flush is awful work with the AA vs Melmac
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A.k.a
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Re: Cultivation General Discussion [Re: Failboat]
#27370199 - 07/01/21 09:29 AM (2 years, 6 months ago) |
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I was thinking about trying the swab syringe thing the other day.
Do you have to use two or could you just swish one around? I was thinking maybe rub it with a loop or scalpel in the water too.
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LAGM2020     
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fahtster
Now With 33%More Faht



Registered: 06/17/06
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Re: Cultivation General Discussion [Re: A.k.a]
#27370220 - 07/01/21 09:36 AM (2 years, 6 months ago) |
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I’ve also just smashed it (when using one, but rubbing two works a lot better) against the glass.. just don’t use too much water
Edited by fahtster (07/01/21 09:37 AM)
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Cultivation General Discussion [Re: A.k.a]
#27370259 - 07/01/21 10:11 AM (2 years, 6 months ago) |
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You could just use one swab.? Two is for breeding.
You could also use a loop or tool, I also did this. But I think for handling and storage and for getting the most out of it, turning a swab into a syringe is the best bet. You could just put a couple of drops from the syring into a plate or something. I just put one drop onto a plate and smeared it with a scalpel. But unless your trying to breed something out I think a drop is enough ha.
-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum... There are known knowns, there are known unknowns, there are also unknown unknowns. With great privilege comes great responsibility.
  Quantom Qups PROOF AND Soft Drops Turn your Swab to a Syringe and Syringe to Multiple Syringes! No Pours (QuantomStyal)Magic Fruit Leather DMT for IandI
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: QM33] 1
#27370326 - 07/01/21 11:01 AM (2 years, 6 months ago) |
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Thanks to everyone for the advice, excuse me for not thanking everyone individually there was a lotta input hahaha but you know who you are. Time to stop spending minutes mangling swab tips now.
Definitely gonna try the sterile water dunk next time thanks Josex. And yeah as you stated the medical grade swabs are much more tightly woven together than regular q tips which is why tweezers have been pretty futile. I totally forgot that you've posted before about how you take swab pieces to BRF pucks like that and usually don't even have to transfer cause the growth is so clean IIRC. Pretty awesome stuff, shoulda remembered when I was tryna snag these fibers lol.
And great point on rubbing the swabs together Faht, I've also seen you describe this before and totally should've remembered when it counted lol. Burying the swab tip always works for me but yeah being down the whole swab isn't my favorite and I've occasionally had contam under the wood still attached to the swab (probably my fault). Gonna try this out for sure next time though.
Gonna try messing with some crosses soon as well and that method of double swabbing and then drying and then rubbing together after wetting seems perfect although I'll probably take it to agar instead of a syringe for now. Still crazy to me that you've done the things you've done without agar, really pushing the envelope, love to see it.
Shroomery is the shit, thanks again to everyone
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PreparationH
apply daily

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Re: Cultivation General Discussion [Re: Lenz]
#27370367 - 07/01/21 11:37 AM (2 years, 6 months ago) |
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Took 3 transfers from the top plate making this the 3rd transfer. Went a little later than I'd have liked so I didn't get the leading edge but Everything is looking good, have lots of colonized plates, 8 oat jars colonizing, and now 3 more transferred 3rd gen plates ready to play with soon.
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