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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
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Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: QM33] * 1
    #27365943 - 06/28/21 07:59 AM (2 years, 6 months ago)

Quote:

Lenz said:
If your SAB is closed up there shouldn't really be much chance of plates contaminating. I feel like it's surprisingly uncommon for contams to pass under the lid and over the wall of the plate even when unwrapped-- unless you leave them somewhere super breezy. Regardless I've never really noticed an increase in contams leaving them undisturbed in an SAB for a bit.

And lumps suck but condensation isn't too big of a deal imo. I'd just pour a bit hotter to avoid lumps and leave them unwrapped in closed SAB for as long as you feel comfortable. I feel like keeping them somewhere warmer usually deals with condensation decently too. Or if you like you could store them upside down then dump out the pooled water on the lid as you open the plate in the SAB. I'd feel a little suspect doing that but you could test a few plates and see if they contam.

Either way hard to get absolutely no lumps and no condensation in an SAB without leaving them to evap a little bit IMO/E.

$300 is ridiculous though sheesh you must not be in the U.S.? They're $115 for me on Amazon.




Found a number of different iterations on celltreat site. Selected 100x15 $143/case.

i'm getting ever better results, but I'm still getting needed experience. I went for the celltreat over the karter sci at $45/case for 90x15 because I slip and open the regular ones too often while handling them.

I have been getting some beautiful plates and a fair number that contam. My solution has been to plan for attrition by doing more transfers than I need of any one thing. If I get no better results from celltreat, the next order will be the karter sci.

I'll up the temp on the water bath a smidge. With the water bath, even if the temp is set right, I gotta chek the water level to make sur it is as high as the agar or the part that sticks up cools.



--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27365958 - 06/28/21 08:14 AM (2 years, 6 months ago)

Look at this shit on reddit. His fancy hood didn't seem to help. "Psilocybin extraction research" lol.

https://www.reddit.com/r/PsilocybinMushrooms/comments/o95jnp/strain_modification_and_hybridization/?utm_medium=android_app&utm_source=share

Snake venom.


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InvisibleMelliferous
πŸŒ΅πŸ„πŸŒ΅πŸ„πŸŒ΅
 Unread Journal

Registered: 10/01/20
Posts: 1,053
Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27366007 - 06/28/21 09:01 AM (2 years, 6 months ago)

Quote:

p9hu7 said:
Look at this shit on reddit. His fancy hood didn't seem to help. "Psilocybin extraction research" lol.

https://www.reddit.com/r/PsilocybinMushrooms/comments/o95jnp/strain_modification_and_hybridization/?utm_medium=android_app&utm_source=share

Snake venom.



Snake venom and CONTAMS!  :puke:


--------------------


"No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Melliferous]
    #27366009 - 06/28/21 09:04 AM (2 years, 6 months ago)

That guy loves bacteria.
Let this serve as an example that fancy equipment is not a stopgap for poor technique and ignorance.

Poor technique and ignorance: the tools with which the reddit mycological community slay.


Edited by Stipe-n Cap (06/28/21 09:15 AM)


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InvisibleMelliferous
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27366034 - 06/28/21 09:22 AM (2 years, 6 months ago)

Quote:

p9hu7 said:
That guy loves bacteria.
Let this serve as an example that fancy equipment is not a stopgap for poor technique and ignorance.

Poor technique and ignorance: the tools with which the reddit mycological community slay.



98% of them at least, I've found a couple gems over there. "I'm a private freelancer. I usually subcontract research from larger firms. I primarily work in cannabis stem cell and psilocybin extraction research." <-- This is gold :rofl:


--------------------


"No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Melliferous]
    #27366035 - 06/28/21 09:23 AM (2 years, 6 months ago)

So full of shit, lol


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OfflineDERRAYLD
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27366037 - 06/28/21 09:26 AM (2 years, 6 months ago)

But that's the shit I produce all the time, can I get a grant or something?


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OfflineAtmozFear
just a shade of myself
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Registered: 01/25/19
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27366039 - 06/28/21 09:27 AM (2 years, 6 months ago)

Quote:

p9hu7 said:
So full of shit, lol



Agree


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27366042 - 06/28/21 09:30 AM (2 years, 6 months ago)

Quote:

DERRAYLD said:
But that's the shit I produce all the time, can I get a grant or something?



lmk, I'll ship you a rattle snake so you can use the venom. :lol:


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OfflineDERRAYLD
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Re: Cultivation General Discussion [Re: LtLurker]
    #27366046 - 06/28/21 09:33 AM (2 years, 6 months ago)

Quote:

LtLurker said:
Quote:

DERRAYLD said:
But that's the shit I produce all the time, can I get a grant or something?



lmk, I'll ship you a rattle snake so you can use the venom. :lol:




Hell yes, I'll contaminate plates without the need for that fancy flow bench.


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OfflinePreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 6 hours, 43 minutes
Re: Cultivation General Discussion [Re: DERRAYLD]
    #27366064 - 06/28/21 09:51 AM (2 years, 6 months ago)

Quote:

p9hu7 said:
So full of shit, lol



Dude's pretty cringe, "I'm just an agent of nature, the mycelium is the miracle."

Lmao.


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OfflineB21
PeaceLoveShrooms
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Registered: 04/09/20
Posts: 871
Loc: Derby City Flag
Last seen: 3 months, 21 days
Re: Cultivation General Discussion [Re: A.k.a]
    #27366075 - 06/28/21 09:56 AM (2 years, 6 months ago)

Quote:

A.k.a said:
I don’t think there’s any scientific evidence but it’s pretty widely accepted that the longer a culture takes to mature the more potent it is.



Primal has a thread about experimenting with keeping colonized tubs from fruiting for a month or more to try and increase potency. Letting cakes and stone jars sit forever is common too.



Edit: found it. Thanks for letting us know about it


--------------------


Edited by B21 (06/28/21 09:58 AM)


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27366079 - 06/28/21 09:59 AM (2 years, 6 months ago)

Quote:

p9hu7 said:
Look at this shit on reddit. His fancy hood didn't seem to help. "Psilocybin extraction research" lol.

https://www.reddit.com/r/PsilocybinMushrooms/comments/o95jnp/strain_modification_and_hybridization/?utm_medium=android_app&utm_source=share

Snake venom.



you know reddit is in a bad spot when you post very clearly bacterial plates, and get nothing but a round of applause and pats on the back. I love reddit, but not for shrooms!


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Munchauzen]
    #27366092 - 06/28/21 10:11 AM (2 years, 6 months ago)

If i wasn't more concerned about the lack of anonimity, i'd be shit posting over there all the time. Everytime i peak I get sad at all the dumb shit those poor newbs are being told.


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OfflinePreparationH
apply daily

Registered: 03/28/05
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Re: Cultivation General Discussion [Re: LtLurker]
    #27366100 - 06/28/21 10:25 AM (2 years, 6 months ago)

the fb page might be worse.




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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: PreparationH]
    #27366205 - 06/28/21 12:14 PM (2 years, 6 months ago)

:lol:  I didn’t realize he’s in front of an oven.  Jesus, he’d freak out if he saw how I inoculate pf jars


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: PreparationH]
    #27366210 - 06/28/21 12:18 PM (2 years, 6 months ago)

God I love the look on that guys face. Laugh out fuckung loud classic.


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InvisibleLadysKnight
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Re: Cultivation General Discussion [Re: mushboy]
    #27366211 - 06/28/21 12:21 PM (2 years, 6 months ago)

He's dead fucking serious.


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
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Re: Cultivation General Discussion [Re: mushboy]
    #27366212 - 06/28/21 12:22 PM (2 years, 6 months ago)

Dude looks like Gru



--------------------
:chems:


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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: PreparationH]
    #27366215 - 06/28/21 12:27 PM (2 years, 6 months ago)

Quote:

PreparationH said:
the fb page might be worse.







Everything is worse on FB.

I know good equipment doesn't make good work. I make killer paintings with Lowes nylon brushes and a garden sprayer. :shrug:

My work is getting cleaner. I think they call it practice.

Speaking of which, is the 'softening' of the rhizomatic growth near the circumference okay? This plate is a clone directly from the stipe:


Here's a close-up



Reddit: The pinnacle of TEKs is uncle ben's. :rofl:


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Cultivation Supplies   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Autoflowering Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Mono Tub Substrate


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