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InvisibleTedsDead
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Registered: 01/03/17
Posts: 4,998
Trusted Cultivator
Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27364247 - 06/26/21 02:42 PM (2 years, 7 months ago)

Quote:

gone-pear-shaped said:
When it comes to the upper part of the scalpel handle, do you get that red hot as well, or just kiss it with the flame?




Not red hot.  I keep an iso soaked rag on hand and whipe the scalpel down between uses.  The residual alcohol will burn when u flame it and that seems to do the trick.  I dont like bods cup method because the blade bends if u let it sit in the cup


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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OfflineLtLurker
Lost Sailor
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Registered: 01/03/18
Posts: 7,535
Loc: Borderlands Flag
Last seen: 6 days, 4 hours
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Re: Cultivation General Discussion [Re: Bonsella]
    #27364252 - 06/26/21 02:46 PM (2 years, 7 months ago)

Quote:

Bonsella said:
No I didn’t use agar, just bought liquid culture syringes online from what looked like a reputable vendor but might be the problem.



They shouldn't be lc's just a spore syringe. Spore to grain is risky. should do agar work for grains.
Quote:

Bonsella said:
I did 90 minutes but my PC only goes up to 11psi. I’ve been seeing some posts saying I should rather go for two hours.

My last run was with uncle bens and obviously no pressure cooker.

I’m also doing the bucket Tek for my substrate but consistently end up with green mold.



2hrs @15psi. You may not have gotten it sterile. Needs plenty longer.

Uncle Bens is trash. It's no surprise it failed.

Bucket tek is correct for coir, you got this bit right.
It's all about getting your spawn right.


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 9 hours
Re: Cultivation General Discussion [Re: LtLurker]
    #27364393 - 06/26/21 05:49 PM (2 years, 7 months ago)

Clean agar will take you far


--------------------
Willpower is the one true virtue



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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27364421 - 06/26/21 06:24 PM (2 years, 7 months ago)

Wow...well injecting LC may not be as quick as i'd hoped but i dnt gotta do it in a sab and i have not had one single jar contaminate so far. Pretty cool.

Verum - Do you put your LC on the stir plate some amount of time b4 you draw from it?
Or does it colonize with a spinning magnet in it?


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OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
Last seen: 4 hours, 1 minute
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27364444 - 06/26/21 07:06 PM (2 years, 7 months ago)

Yeah can someone familiarize me with these magnetic stirrers?  Do you put a magnet inside the jar with the LC?  Do you leave it on for hours and/or days?  I get a boner looking at new lab equipment on Amazon :stonedjerk:


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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: AtmozFear]
    #27364463 - 06/26/21 07:41 PM (2 years, 7 months ago)

Quote:

AtmozFear said:
Yeah can someone familiarize me with these magnetic stirrers?  Do you put a magnet inside the jar with the LC?  Do you leave it on for hours and/or days?  I get a boner looking at new lab equipment on Amazon :stonedjerk:




here ya go
don't waste the wood

https://www.amazon.com/gp/product/B07FP8J8YV/ref=ppx_yo_dt_b_asin_title_o09_s00?ie=UTF8&psc=1


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27364469 - 06/26/21 07:54 PM (2 years, 7 months ago)

Oh that’s a cool one!  So how are you supposed to keep an LC from getting contaminated cuz it looks like you have to open the top… and btw can you keep unused agar liquified with that particular stirrer?


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OfflineCocaineBuffet
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Registered: 08/29/19
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Re: Cultivation General Discussion [Re: AtmozFear]
    #27364486 - 06/26/21 08:03 PM (2 years, 7 months ago)

I have had LC sitting stagnant for months and it was clean when I pulled the syringe and tested it on agar. I wouldn't recommend it because it will get so thick it won't fit through the needle. In my experience if your LC is prepped correctly and it is inoculated with a clean inoculant you don't need to have it sitting on stir plate every day.


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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: CocaineBuffet]
    #27364506 - 06/26/21 08:22 PM (2 years, 7 months ago)

I’m at the GF’s. Dehydrator turns off in a couple hours. House is 47-50% humidity. Can I turn the dehydrator back on when I get home in the AM, or are they weakened from rehydrating to the point of tossing them? Ai’nt the end of the world. Thanks to your help I’ve pulled about 3 lbs wet this week and tubs are still producing. :tongue2:

tyia


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: AtmozFear]
    #27364553 - 06/26/21 09:18 PM (2 years, 7 months ago)

Quote:

AtmozFear said: I get a boner looking at new lab equipment on Amazon :stonedjerk:





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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: AtmozFear]
    #27364575 - 06/26/21 09:39 PM (2 years, 7 months ago)

Quote:

AtmozFear said:
Oh that’s a cool one!  So how are you supposed to keep an LC from getting contaminated cuz it looks like you have to open the top… and btw can you keep unused agar liquified with that particular stirrer?




I just got it the other day. I have to read the teks on using one for LC. I'm going to use it to prep my agar for Pcing. Fucker goes to over 700F. Planning not to have to stare into a fucking microwave with dits all over the glass.

To keep agar at the right temp to pour, I have a SAB so I hit about 47.8 c I love my water barh:

https://www.amazon.com/VEVOR-300W%EF%BC%8CHeating-Thermostatic-3L%EF%BC%8CStainless-Bath%EF%BC%8CLaboratory/dp/B089Q4ZWSL/ref=mp_s_a_1_4?dchild=1&keywords=vevor+water+bath&qid=1624764989&sprefix=vevor+water+ba&sr=8-4


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 2 months, 25 days
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27364624 - 06/26/21 10:24 PM (2 years, 7 months ago)

Quote:

Feasoghorm said:
Wow...well injecting LC may not be as quick as i'd hoped but i dnt gotta do it in a sab and i have not had one single jar contaminate so far. Pretty cool.

Verum - Do you put your LC on the stir plate some amount of time b4 you draw from it?
Or does it colonize with a spinning magnet in it?




Verum doesn't use syringes anymore, he free pours. :hereyougo:


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Mr. Mushie]
    #27364639 - 06/26/21 10:45 PM (2 years, 7 months ago)

Quote:

Feasoghorm said:
Verum - DID you put your LC on the stir plate some amount of time b4 you draw from it?
Or does it colonize with a spinning magnet in it?





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OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
Last seen: 4 hours, 1 minute
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27364657 - 06/26/21 11:10 PM (2 years, 7 months ago)

:lmafo: I was away for six months, nice to see all u guys again!!  So many personalities on here


--------------------


Edited by AtmozFear (06/26/21 11:11 PM)


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OfflineBonsella
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Posts: 13
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Re: Cultivation General Discussion [Re: LtLurker]
    #27364741 - 06/27/21 12:30 AM (2 years, 6 months ago)

Thanks for the feed back. I’m going to follow your advice and order some spore syringes and premade agar plates.  Hopefully I’ll get closer to success 🤞🤞🤞🤞


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OfflinePreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 8 hours, 38 minutes
Re: Cultivation General Discussion [Re: Bonsella]
    #27364809 - 06/27/21 03:15 AM (2 years, 6 months ago)

Used this plate and transferred to 8 grain jars.  I had 9 jars but when I got done... I couldnt find the wedge in the 9th jar lol.  I dunno if I doubled one up, the wedge was hidden, or just totally skipped that one in general



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InvisibleWyoMX
I'm a teapot User Gallery
Registered: 07/06/15
Posts: 2,102
Loc: PNW
Re: Cultivation General Discussion [Re: SpaceBaby]
    #27364815 - 06/27/21 03:33 AM (2 years, 6 months ago)

Quote:

SpaceBaby said:
I’m at the GF’s. Dehydrator turns off in a couple hours. House is 47-50% humidity. Can I turn the dehydrator back on when I get home in the AM, or are they weakened from rehydrating to the point of tossing them? Ai’nt the end of the world. Thanks to your help I’ve pulled about 3 lbs wet this week and tubs are still producing. :tongue2:

tyia




You should be fine. My roommate unplugged the dehydrator when he left for work at 5am but didn't jar em up just left them on the trays. I woke up at around noon (I work into the morning hours I'm not just a lazy mofo I swear :lol: ) and plugged it back in for a couple hours and all was well for me. Didn't notice any potency issues when I ate that batch. My area is only around 30% humidity but I don't imagine that being a deal breaker.


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OfflineSockadin
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Registered: 01/03/10
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Re: Cultivation General Discussion [Re: WyoMX]
    #27364848 - 06/27/21 05:28 AM (2 years, 6 months ago)

Yeah it wont hurt them.

:whathesaid:

I have left mushrooms in the dehydrator for days. Still had good potency.


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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
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Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: Sockadin]
    #27364858 - 06/27/21 05:43 AM (2 years, 6 months ago)

Quote:

WyoMX said:
Quote:

SpaceBaby said:
I’m at the GF’s. Dehydrator turns off in a couple hours. House is 47-50% humidity. Can I turn the dehydrator back on when I get home in the AM, or are they weakened from rehydrating to the point of tossing them? Ai’nt the end of the world. Thanks to your help I’ve pulled about 3 lbs wet this week and tubs are still producing. :tongue2:

tyia




You should be fine. My roommate unplugged the dehydrator when he left for work at 5am but didn't jar em up just left them on the trays. I woke up at around noon (I work into the morning hours I'm not just a lazy mofo I swear :lol: ) and plugged it back in for a couple hours and all was well for me. Didn't notice any potency issues when I ate that batch. My area is only around 30% humidity but I don't imagine that being a deal breaker.



Quote:

Sockadin said:
Yeah it wont hurt them.

:whathesaid:

I have left mushrooms in the dehydrator for days. Still had good potency.




Thanks, you two. Great news. I hate tossing the babies.

:bow2:


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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Offlinerockyfungus
dirty
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Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 10 hours
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27364863 - 06/27/21 05:50 AM (2 years, 6 months ago)

Quote:

Feasoghorm said:
Wow...well injecting LC may not be as quick as i'd hoped but i dnt gotta do it in a sab and i have not had one single jar contaminate so far. Pretty cool.

Verum - Do you put your LC on the stir plate some amount of time b4 you draw from it?
Or does it colonize with a spinning magnet in it?




I give LC a swirl by hand every few days never a problem, you can also pour LC if you got steady hands. I throw my LC out after this... syringe is better if you want to reuse that LC.

GLC is another fun one to pour and play with.


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