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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 18 hours
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Re: Cultivation General Discussion [Re: tombosley8]
#24116801 - 02/24/17 05:53 PM (6 years, 10 months ago) |
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Quote:
tombosley8 said: Thanks so much for everyone's help with this... I ended up ordering this one https://www.globalindustrial.com/p/hvac/filters/airex-filters/airex-174-36-x48-x6-hepa-filter-micro-air-99-97-efficiency-metal-frame-high-quality but I accidentally ordered the wood frame instead of metal.
Any downside to this?
Don't think so, the wooden one might slide into place easier.
I wish I had room for a hood this size!
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 Amateur Mycologists United AMU Q&A
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: mushpunx]
#24117003 - 02/24/17 06:56 PM (6 years, 10 months ago) |
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Hey guys,
when you do G2G, what do you do with the agar piece in your master jar? Just don't care about if it gets into one of the receiving jars or do you try to tilt the jar a way the agar piece stays in the master jar?
I grain 2 grained the agar pieco into on of the receiving jars all the time but now I am worried if this is the correct way.
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24117011 - 02/24/17 06:59 PM (6 years, 10 months ago) |
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that's how I roll
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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yeah I thought it's not a problem at all, just wanted to ask.
Since the master jar should be clean and the receiving jar should be clean, the agar wedge won't do anything to the cleanliness of the process - but better be safe than sorry! :P
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24117055 - 02/24/17 07:11 PM (6 years, 10 months ago) |
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I would actually want the agar in one of the jars if it didn't take too much effort to put it in. The more the merrier.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 18 hours
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24117063 - 02/24/17 07:13 PM (6 years, 10 months ago) |
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Quote:
AlCapone2k said: Hey guys,
when you do G2G, what do you do with the agar piece in your master jar? Just don't care about if it gets into one of the receiving jars or do you try to tilt the jar a way the agar piece stays in the master jar?
I grain 2 grained the agar pieco into on of the receiving jars all the time but now I am worried if this is the correct way.
Regards
Yea its fine. When I spawn my tubs I pick it out if I see it, for no particular reason
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 Amateur Mycologists United AMU Q&A
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: mushpunx]
#24117069 - 02/24/17 07:15 PM (6 years, 10 months ago) |
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Quote:
mushpunx said:
Quote:
AlCapone2k said: Hey guys,
when you do G2G, what do you do with the agar piece in your master jar? Just don't care about if it gets into one of the receiving jars or do you try to tilt the jar a way the agar piece stays in the master jar?
I grain 2 grained the agar pieco into on of the receiving jars all the time but now I am worried if this is the correct way.
Regards
Yea its fine. When I spawn my tubs I pick it out if I see it, for no particular reason 
yeah, I'll do the same Just to have it done!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: AlCapone2k] 1
#24117162 - 02/24/17 07:58 PM (6 years, 10 months ago) |
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Is a random agar plate of spores ever gonna do better than a a clone?
I don't quite understand the rhyme or reason to transferring, cloning, etc. I mean, I'm doing it, and getting results, but am not sure why.
Am I wasting my time keeping this KSSS going for over 6 months, 7,8 transfers, vs a agar KSSS pin clone?
Are the genetics the same from the 6th transfer as the 8th?
My tub in sig was spawned with 5th gen transfer KSSS spores. I just spawned a tub the other day with an 8th gen transfer plate. Gonna be "similar" results, or quite different? Am I keeping the same shit going, good or bad, or does it change with each transfer, until I isolate?
Thanks for any help.
-------------------- KSSS And PE WBS.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Moabfighter] 1
#24117208 - 02/24/17 08:15 PM (6 years, 10 months ago) |
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Quote:
Moabfighter said: Is a random agar plate of spores ever gonna do better than a a clone?
I don't quite understand the rhyme or reason to transferring, cloning, etc. I mean, I'm doing it, and getting results, but am not sure why.
Am I wasting my time keeping this KSSS going for over 6 months, 7,8 transfers, vs a agar KSSS pin clone?
Are the genetics the same from the 6th transfer as the 8th?
My tub in sig was spawned with 5th gen transfer KSSS spores. I just spawned a tub the other day with an 8th gen transfer plate. Gonna be "similar" results, or quite different? Am I keeping the same shit going, good or bad, or does it change with each transfer, until I isolate?
Thanks for any help.
Yes, a random collection of spores can outperform a clone. A clone by nature is not an inherently better choice, it's simply limited genetics of whatever you cloned from. For instance, I can clone a shitty fruit and I'd likely have those shitty fruits in my next tub. But with MS, there's so many strains present, the chances of having all shitty genetics is super low.
The reason for transferring and cloning is that you can guarantee specific results. In my example above, I cloned a shitty fruit. In real practice, you'd clone something like the biggest mushroom from the first big cluster in a tub of MS. That's isolating towards proven fruiterers with desired genetics. That can very often outperform the crap shoot of MS.
An isolate is just a single strain of mushroom mycelium. It can be worth doing to find an amazing specimen, but time consuming. Many people just do MS because you're almost guaranteed results. If you start from a clone, your genetics are limited. Let's say you can see 10 sectors in the clone plate. If you fruit out all of those 10, some can be non-fruiting, some will do well, some may do amazing.
You're definitely limiting genetics with each transfer, but how much is hard to tell. There could still be many strains present. If your 5th transfer is giving you awesome results, I'd store that and just keep making plates from it. If your goal is to whittle down to isolates and see which fruits the best, keep going.
But if you're just transferring along, not saving earlier plates, you're definitely limiting the gene pool. And you could easily wind up with the genetics that don't perform well. This is why people store proven cultures long-term and just go back, take a small piece of it, and grow a new plate with those genetics.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: stareatclouds] 1
#24117212 - 02/24/17 08:16 PM (6 years, 10 months ago) |
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Quote:
stareatclouds said:
Quote:
Moabfighter said: Is a random agar plate of spores ever gonna do better than a a clone?
I don't quite understand the rhyme or reason to transferring, cloning, etc. I mean, I'm doing it, and getting results, but am not sure why.
Am I wasting my time keeping this KSSS going for over 6 months, 7,8 transfers, vs a agar KSSS pin clone?
Are the genetics the same from the 6th transfer as the 8th?
My tub in sig was spawned with 5th gen transfer KSSS spores. I just spawned a tub the other day with an 8th gen transfer plate. Gonna be "similar" results, or quite different? Am I keeping the same shit going, good or bad, or does it change with each transfer, until I isolate?
Thanks for any help.
Yes, a random collection of spores can outperform a clone. A clone by nature is not an inherently better choice, it's simply limited genetics of whatever you cloned from. For instance, I can clone a shitty fruit and I'd likely have those shitty fruits in my next tub. But with MS, there's so many strains present, the chances of having all shitty genetics is super low.
The reason for transferring and cloning is that you can guarantee specific results. In my example above, I cloned a shitty fruit. In real practice, you'd clone something like the biggest mushroom from the first big cluster in a tub of MS. That's isolating towards proven fruiterers with desired genetics.
An isolate is just a single strain of mushroom mycelium. It can be worth doing to find an amazing specimen, but time consuming. Many people just do MS because you're almost guaranteed results.
You're definitely limiting genetics with each transfer, but how much is hard to tell. There could still be many strains present. If your 5th transfer is giving you awesome results, I'd store that and just keep making plates from it. If your goal is to whittle down to isolates and see which fruits the best, keep going.
Good ass reply that helped my wrap my head around it better. Thanks man.
-------------------- KSSS And PE WBS.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Cultivation General Discussion [Re: Moabfighter]
#24117235 - 02/24/17 08:23 PM (6 years, 10 months ago) |
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I second that. great reply
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: tombosley8]
#24118611 - 02/25/17 11:29 AM (6 years, 10 months ago) |
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Quote:
tombosley8 said: so does that .02% actually play a role? like out of every 10000 inoculations 2 would contam due to the filter?
Look up 99.97 vs 99.99
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: bodhisatta]
#24118685 - 02/25/17 12:04 PM (6 years, 10 months ago) |
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Two weeks old tomorrow. Is it safe to use these for LI tonight?
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Those look very close to isos, I would take transfers to new plates for further isolating before doing anything else with those
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Quote:
PinPornProducer said: Those look very close to isos, I would take transfers to new plates for further isolating before doing anything else with those
I have 4 more of each just like these. I have dishes ready for transfer. Right on. Thanks x3P
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 4 hours
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Re: Cultivation General Discussion [Re: bodhisatta]
#24119164 - 02/25/17 03:53 PM (6 years, 10 months ago) |
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Quote:
bodhisatta said:
Quote:
tombosley8 said: so does that .02% actually play a role? like out of every 10000 inoculations 2 would contam due to the filter?
Look up 99.97 vs 99.99 
I did and that was the gist of what I got out of it but I may be way off...
either way I ended up taking your advice and going with a 99.99% that was a fair amount cheaper on ebay. http://catalog.tfsi1.com/Documents/MICROGUARD%2099.pdf the 36 x 48 model. http://www.ebay.com/itm/361177874783 $350 with shipping! weird thing was I searched ebay for days and couldn't find anything and then accidentally stumbled upon it while looking up specs for the one I had decided on before. Again thanks for everyone's help with clarifying all the details and really making the decision a million times easier. That's priceless.
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Edited by tombosley8 (02/25/17 07:39 PM)
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 17 hours, 33 minutes
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Re: Cultivation General Discussion [Re: tombosley8]
#24119582 - 02/25/17 07:34 PM (6 years, 10 months ago) |
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Does anybody know if A+ Albino is a slow to fruit strain? I'm having trouble getting a test tub to fruit. I may have over watered it, but looking for feedback. Thank you.
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Shadowboxing the apocalypse and wandering the land
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Cultivation General Discussion [Re: Crispykoot]
#24119585 - 02/25/17 07:35 PM (6 years, 10 months ago) |
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Pretty aggressive fruiters imo
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Are wide mouth quart mason jars shorter than regular mouth? I've no way to check that, they don't sell those jars here.
I imported mason jars regular mouth from the land of freedom a while back, the thing is that I can't possibly fit as many jars in my pc as the manufacturer says, bummer.
Edited by Josex (02/25/17 08:54 PM)
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: Josex]
#24119724 - 02/25/17 08:53 PM (6 years, 10 months ago) |
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Dude every brand is a different size. I have like 4 or 5 different brands of mason jars and one random wide mouth.
I find bernardin to be the best for stuffing a PC
Golden harvest are too fat but a solid second contender and still fit. I got tall slender ones and even slightly taller ones than that (forget the names)
I think I have a ball brand but I'd have to check
I believe the wide mouth bernardin are the same height as the reg mouth tho
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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