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OfflineLeafyClark
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Registered: 02/14/19
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Re: Cultivation General Discussion [Re: PreparationH]
    #27363315 - 06/25/21 07:53 PM (2 years, 7 months ago)

Quote:

PreparationH said:
This is a second transfer plate, best looking one of the bunch, I plan to transfer wedges into 9 jars tonight





you could make a quick an easy LI and inoculate 10 jars off that plate with a lot faster colonization times if ya wanted


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InvisiblemushboyMDiscord
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Registered: 04/24/05
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Re: Cultivation General Discussion [Re: LeafyClark]
    #27363328 - 06/25/21 08:00 PM (2 years, 7 months ago)

I'd take another transfer. I'd also inoculate some jars with the remaining myc:awesomenod:


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OfflinePreparationH
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Re: Cultivation General Discussion [Re: mushboy]
    #27363341 - 06/25/21 08:05 PM (2 years, 7 months ago)

Yea I will go to another plate too, I have 4 other plates that look similar too, I figured I'd just take 9 wedges to jars, use other plates to make more transfers and just keep it moving


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OfflineLeafyClark
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Re: Cultivation General Discussion [Re: PreparationH]
    #27363346 - 06/25/21 08:09 PM (2 years, 7 months ago)

wedge to grain is just so slow compared to LI, i just did a few the other day for the first time in forever since learning about LI and i just dont miss it. the one time shake is a deal breaker for me to :shrug:


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27363348 - 06/25/21 08:10 PM (2 years, 7 months ago)

Verum - Stir plate. How do you handle that anyway? Turn it on like 10 min b4 drawing a syringe, or does it grow with a spinning magnet?

Sockadin - That wud be a bummer supreme cuz these things are mostly shell. I was under the impression that the shells where a significant enough part of the food source. Hmm...

Thank you for responding, guyz.


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27363350 - 06/25/21 08:12 PM (2 years, 7 months ago)

I just came across black sfs today. They wanted $45 for 30 lbs. I think I will stick with the RGS for now.


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OfflineLeafyClark
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Re: Cultivation General Discussion [Re: BrownBear]
    #27363355 - 06/25/21 08:14 PM (2 years, 7 months ago)

wheat for the win, 13 smackaroos for 50 pounds. and its fast :thumbup:


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OfflinePreparationH
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Re: Cultivation General Discussion [Re: LeafyClark]
    #27363366 - 06/25/21 08:21 PM (2 years, 7 months ago)

I've done a blenderless LI before, I probably will do it with another batch but I feel like it's one more vector, I'm also not in any real rush. 

Once I am successful with this batch of jars I'll start fucking around more.  Honestly 9 quarts of spawn... No matter what... I will end up with too much stuff because I'll take one or two of these and g2g them.  Also I have 5 more plates going too lol.


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: LeafyClark]
    #27363395 - 06/25/21 08:36 PM (2 years, 7 months ago)

Quote:

LeafyClark said:
wheat for the win, 13 smackaroos for 50 pounds. and its fast :thumbup:




I wish I could get wheat consistently. I would have to travel over an hour for it though. On top of that, it isn't available all year around.

I am currently changing my process. I want to grow straight out of bags. I am going to be supplementing them with rice bran and only using the RGS to inoculate them. RGS has one of the, if not the most, inoculation points per gram of seed than any other that we use in this hobby. So a little bit goes a long way.


Edited by BrownBear (06/25/21 08:37 PM)


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: skinnylittleblonde]
    #27363460 - 06/25/21 09:26 PM (2 years, 7 months ago)

Quote:

skinnylittleblonde said:
Thank You!! I’ve read conflicting data & my gut was saying’ ‘leave it be’ but I didn’t want to screw the whole pooch Thanks Again!



lol. you gonna screw the pooch piecemeal?


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: PreparationH]
    #27363471 - 06/25/21 09:40 PM (2 years, 7 months ago)

Oh i am the wheat fucking master. Jst wanted to do some sfs. But fuck it. Every time i fuck with something else it's a damn issue. i luv me some wheat :jah:


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Offlinegabbk
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27363558 - 06/25/21 11:46 PM (2 years, 7 months ago)


God fucking damnit:mad2::mad2::mad2::mad2::mad2:
I'm having a rough time with this shit😡😡
All my life I've been cooling down the scalpel after flaming red hot and now these days I'm getting this bacteria on the same slit on the agar (the pattern of the cut of the blade).
Now it makes me thing all my entire life Ive been carrying with this shit and realized it these days right when started I changing to more clear recipes
What should I do to fix this?
I'm pcing the agar 30 mins at 20psi and making really sure the blade is red hot before inoculating or transferring. Literally half of my agar sessions I'm with the arm up sterilizing the blade waiting for the blade at least five secs being red so this shit is getting me frustrated a lot 😡


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: gabbk]
    #27363562 - 06/25/21 11:58 PM (2 years, 7 months ago)

Are u wiping it with iso befpre flaming each time?


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


Edited by TedsDead (06/25/21 11:59 PM)


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: TedsDead]
    #27363763 - 06/26/21 06:38 AM (2 years, 7 months ago)

Are you flaming it down to the handle? That fixed a lot for me, I used to only flame to where it slides
On the handle.


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LAGM2020


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Offlinegabbk
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Re: Cultivation General Discussion [Re: A.k.a]
    #27363809 - 06/26/21 07:42 AM (2 years, 7 months ago)

Thanks for helping guys
Yes I start each session flaming the handle and then focusing just on the blade.. I mean I just do the handle the first transfer. Do you do it in between all transfers Aka?
@TedsDead: no Ted I've never cleaned it on alcohol in between uses.. although I saw it once on a bods video that he did have a glass of alcohol on hand to do it .. I didn't know it was necessary. I'll definitely start to use it and see if it improves, thanks for that tip:thumbup:

Last night I checked all my plates and interestingly what I've shown is present in almost every plate of the LAST batch, so I think it could be the this last particular sets of Petris maybe that perhaps came badly sterilized???
idk really, some of the plates of the previous batches (not this last one) also have this bacterial pattern on the way of the scalpel, and some others don't:confused:
Is there any possibility it could be the  the type of gas? I use a mix of butane and propane for the torch (I know it's silly but I just wanna make sure haha)

Maybe I'll also stop cooling down the blade on the agar. I learnt it from a video of RR and since then I did it always
.. do anyone bothers cooling down the blade? I'm afraid to kill the donor myc if I directly cut the agar without cooling it before. Any experiences?

I really dont know where to aim, where's the issue:mad2:


Edited by gabbk (06/26/21 07:57 AM)


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: gabbk]
    #27363821 - 06/26/21 07:55 AM (2 years, 7 months ago)

Not the handle but the part of the blade where it locks on. I usually iso the handle when I start and then just flame the entire blade piece between plates.



When I started out I was only flaming the solid part of the blade and had problems that went away once I started doing the entire part where it connects.


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Offlinegabbk
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Re: Cultivation General Discussion [Re: A.k.a]
    #27363831 - 06/26/21 08:02 AM (2 years, 7 months ago)

Ohh gotcha aka I'll stop bothering with the handle then :lol:

Also, is it necessary to cool down the blade on the agar prior to transfer? Maybe I'm adding vectors of contamination.. I just do it cause I'm afraid to kill the donor mycelium if I cut it right away


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27363857 - 06/26/21 08:26 AM (2 years, 7 months ago)

Alright, Feaso and all of you who poked me about oats. Oats may be kicking my ass, which sucks since I'd rather be the Top.

What is you favoirte grain, and why?


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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Offlinegone-pear-shaped
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Registered: 10/30/17
Posts: 822
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Re: Cultivation General Discussion [Re: A.k.a]
    #27363875 - 06/26/21 08:48 AM (2 years, 7 months ago)

When it comes to the upper part of the scalpel handle, do you get that red hot as well, or just kiss it with the flame?


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: gone-pear-shaped]
    #27363890 - 06/26/21 08:58 AM (2 years, 7 months ago)

Since this is quick Q&A

dafuq?

I remember you said the "little hats" were a common mute, but what's up with the fuzz
and more importantly is the word coloration thing s bad thing??





--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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