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InvisibleWyoMX
I'm a teapot User Gallery
Registered: 07/06/15
Posts: 2,102
Loc: PNW
Re: Cultivation General Discussion [Re: Eugene Gesuale]
    #27348096 - 06/14/21 03:58 PM (2 years, 7 months ago)

I prefer the biggest one from a good cluster. I've taken some from some big chonk boys but have never been to impressed from them, would give me good size mushrooms but never got the pinset I've gotten when I clone clusters. I'm fairly new to cloning have only take and ran 15-20 so not super experienced but that's my 2cents.


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OfflineFailboat
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Re: Cultivation General Discussion [Re: WyoMX]
    #27348131 - 06/14/21 04:27 PM (2 years, 7 months ago)

Clone both, run em, keep what's your preference. Refrigerate the master culture before you lose it.


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InvisibleLenz
Misunderestimated
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Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: Cultivation General Discussion [Re: PrimalSoup]
    #27348604 - 06/14/21 11:47 PM (2 years, 7 months ago)

Quote:

PrimalSoup said:
Quote:

Lenz said:
Quote:

PrimalSoup said:
If the filters get wet through that's what can happen - mold germinates on the top of the filter and grows through to the inside where it releases spores.  Been there, done with that - cover the lids with foil to keep them from being saturated. :cookiemonster:




I thought the PTFE filters were hydrophobic? I wasn't using foil for a bit cause I heard it's not necessary with SFD's but went back to using it to see if that changed my contam rate at all. No such luck unfortunately.







Hydrophobic won't save your bacon if they're wet.  Especially if there's any sort of nutrient in them to draw the mold.  I was losing about half of my jars to this problem at one time before I figured it out, but what I actually changed was I went from a full filter under a cap with a hole to a small square RTVd on top of the cap.

Even with that I was still getting some mold occasionally, so I got some silicone gaskets for under the lids. THAT was the key to no infiltration.

IME foil isn't needed if you inoculate immediately but I rarely do that, and with the foil on keeping crap out they can sit around anywhere for weeks - won't dry out and will be cleaner when you go to innoc.  I've left jars sitting out for months with no problem. :lol:

Here's what I mean: 

You could do the lid with plastic wrap when you take it out of the PC, probably cheaper than foil but not as durable.

Anyway, because you say it starts from the top that means it's sneaking in from somewhere at the top - eliminate all the vectors. :shrug:




What did it look like when the mold was growing on your SFD? Was it very obvious? I've done the RTV'd SFDs before and still had similar issues, I switched to whole SFD's trying to fix this. It would somewhat explain the fact that jars that I've shaken once I removed them from the PC still have contamination start at the top but I don't think I've really noticed my SFDs being particularly moldy when my jars contaminate.

It does make more sense the more I think about it though for a few reasons.

1. I leave my jars to cool in the PC overnight after cooking them, which means that even if there is foil covering the SFD's they're basically getting soaked from being left in such a moist environment over such a time period.
2. I shake them once I remove them from the cooker which basically gets the nutritious grain water all over the entire area of the disk which I believe soaks in and suffuses the already wet disk readily.
3. They aren't able to dry out quickly enough to prevent mold growth because it's trapped under the lid of the jar and the only area of the disk exposed to open air is the small 1/4 inch hole drilled in the lid.

If there's any part of this line of reasoning that doesn't hold together please let me know, I think maybe point 3 is shaky since I thought SFDs are supposed to dry out pretty quickly but I really don't know. Otherwise I think at this point the only 2 possible options are either mold growth on the SFD or the lid itself letting something in. I think it's almost entirely impossible that a brand new stack of SFD's from a reputable source is faulty, so I'm going to rule that out for now.

It still doesn't answer the question of previous contamination with different lid setups, like unmodded or RTV'd cut to size SFDs but I'm going to take this one step at a time because at this point my head is fucking swirling with all the little possibilities and what ifs at this point.

Thank you for the input and excuse the wordy response, just trying to get this all sorted in my head.


--------------------
:chems:


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OnlineDERRAYLD
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Re: Cultivation General Discussion [Re: Lenz]
    #27348631 - 06/15/21 12:29 AM (2 years, 7 months ago)

I left some poured grain water, yeast, agar plates in the cupboard un-pc'ed  for a day while I got to them, that was a bad idea, slimy mess.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: DERRAYLD] * 1
    #27348699 - 06/15/21 03:11 AM (2 years, 7 months ago)

As soon as the pressure drops to zero i remove the lid, tighten the jar lids b4 i remove them, set them on my counter and remove all the foil. Then i let them sit overnight to absord most of the water. After that i shake em up. No problem.
I jst use them cheap sfd stickers off amazon. Stuck to top of lid over 1/4" hole.


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27348894 - 06/15/21 07:51 AM (2 years, 7 months ago)

Quote:

Feasoghorm said:
As soon as the pressure drops to zero i remove the lid, tighten the jar lids b4 i remove them, set them on my counter and remove all the foil. Then i let them sit overnight to absord most of the water. After that i shake em up. No problem.
I jst use them cheap sfd stickers off amazon. Stuck to top of lid over 1/4" hole.




Yeah, I've been getting some bacteria in almost every jar. 3/8-inch hole config, rubber side up, covered with SFD, ring screwed on loosely, then foil. The stack order with the SFD on top, though not the hole, are per RR grain vid. PCd and left to cool overninght. [I leave them overnight in the PC, partly because I finish the run after I should be asleep.]

I'm going to try not leaving them to cool overnight and immediately removing them, screwing the fuckers down and letting them sit on the counter overnight without the foil. I might also try a 1/4-inch hole if I still have problems, though I doubt that is the vector.

If that doesn't work, perhaps siliconed SFDs...


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OfflineSpaceBaby
alchemist
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27348897 - 06/15/21 07:53 AM (2 years, 7 months ago)

Quote:

DERRAYLD said:
I left some poured grain water, yeast, agar plates in the cupboard un-pc'ed  for a day while I got to them, that was a bad idea, slimy mess.




I had 11 jars of oats, so I didn't PC one right away and was going to make up another batch of oats. Didn't get to it for a couple of days, had to open the jar in bleach water and clean it out.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OnlineDERRAYLD
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27348898 - 06/15/21 07:53 AM (2 years, 7 months ago)

Are you venting?
Are you pc'ing for long enough?


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OfflineSpaceBaby
alchemist
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27348903 - 06/15/21 07:58 AM (2 years, 7 months ago)

Quote:

DERRAYLD said:
Are you venting?
Are you pc'ing for long enough?




Presto 23 qt
venting 15 minutes
PC run 2h15min [Not comfortable going much longer without an AA.]

:shrug:


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OnlineDERRAYLD
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27348924 - 06/15/21 08:20 AM (2 years, 7 months ago)

Odd check your filters in that case, also confirm your grain prep is good.


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27348965 - 06/15/21 08:52 AM (2 years, 7 months ago)

Quote:

DERRAYLD said:
Odd check your filters in that case, also confirm your grain prep is good.




SFDs from The Mycologist in Olympia WA - not a sponsor. I can't imagine they are not good.

I'm hearing get the fuck away from oats, but I do like the difficulty :rolleyes:
Definitely boiled right and dried well. I extended the drying on the most recent batch of jars and noccd them with clean [T3or4] Huatla agar wedges.

This last batch of oats I dried for even more hours, this time a thin layer over about a 15 sq ft area move about regularly on a tarp and then put in collanders in the fridge overnight.

That said, 30-quarts of oats colonizing not withstanding, I am way new at this.

Thought I might try another grain. Lots of WBS talk lately, but RR vid is on rye berries. Is his exact TEK in his grain-prep video still considered current or is it outdated?

On a general note, I love so many of the names we pick...


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleMr Piggy
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27348973 - 06/15/21 08:57 AM (2 years, 7 months ago)

WBS has never failed me.  Very easy to prep and produced some AMAZING flushes.


--------------------
🅃🄴🄰🄼 🄵🄾🄸🄻


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OfflineEugene Gesuale
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27348975 - 06/15/21 08:57 AM (2 years, 7 months ago)

If I decapitate a few that are ready to print how long will the stipes survive before harvest


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: Mr Piggy]
    #27348979 - 06/15/21 08:59 AM (2 years, 7 months ago)

Quote:

Mr Piggy said:
WBS has never failed me.  Very easy to prep and produced some AMAZING flushes.




Thanks, Mr Piggy: Got a brand, source, URL?

I am moving to all organic everything.

I'm sure there are a fuckton of TEKs for WBS.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleMr Piggy
Big Dick Retard
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Re: Cultivation General Discussion [Re: Eugene Gesuale]
    #27348980 - 06/15/21 09:00 AM (2 years, 7 months ago)

:shrug:  I dunno, fuck around and find out?

Do it for the public knowledge!

:popcorn:


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InvisibleMr Piggy
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27348983 - 06/15/21 09:02 AM (2 years, 7 months ago)

I just grab the nine dollar big bag at our local market called bi-mart.

Not gonna lie, organic bird seed is going to be fucking expensive if you can even find it.  I'm an organics nut in most things in life, but grain spawn is just too damn expensive when organic.  I'm not going to tell you what to do, but personally I would not worry that much on it.  Myc destroys many things.


--------------------
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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27348988 - 06/15/21 09:04 AM (2 years, 7 months ago)

Quote:

SpaceBaby said:
Quote:

DERRAYLD said:
Odd check your filters in that case, also confirm your grain prep is good.




SFDs from The Mycologist in Olympia WA - not a sponsor. I can't imagine they are not good.

I'm hearing get the fuck away from oats, but I do like the difficulty :rolleyes:
Definitely boiled right and dried well. I extended the drying on the most recent batch of jars and noccd them with clean [T3or4] Huatla agar wedges.

This last batch of oats I dried for even more hours, this time a thin layer over about a 15 sq ft area move about regularly on a tarp and then put in collanders in the fridge overnight.

That said, 30-quarts of oats colonizing not withstanding, I am way new at this.

Thought I might try another grain. Lots of WBS talk lately, but RR vid is on rye berries. Is his exact TEK in his grain-prep video still considered current or is it outdated?

On a general note, I love so many of the names we pick...



SFDs are not fail safe. Been screwed before by them. Spent a year changing
every aspect of my cult and the filters were the last thing I checked
cause they were SFDs and I didnt think they could possibly be it.

Lotta work for a grain prep. Switch to wheat and accept wheats loving grace brother


--------------------


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: natedawgnow]
    #27348992 - 06/15/21 09:08 AM (2 years, 7 months ago)

ive had those sfd stickers fail on plastic lids/media bottle caps. they seem to work on metal lids so far but i stilled covered the sticker with a layer of mp tape.


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InvisibleJosex
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Re: Cultivation General Discussion [Re: mushboy]
    #27349001 - 06/15/21 09:13 AM (2 years, 7 months ago)

That's cuz glue doesn't belong on a lid.  :dancinghitler:

Willing to bet most cases of filter failure is actually old, defective or poorly applied silicone, glue or whatever you used.

Stuffed poly ftw, or else, whole sfd for 2 pieces lids.


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OfflinePreparationH
apply daily

Registered: 03/28/05
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Re: Cultivation General Discussion [Re: Josex]
    #27349005 - 06/15/21 09:17 AM (2 years, 7 months ago)

Ok so my control plate 7 days out has zero contamination and the one plate I snapped the spore swab off into is growing mycelium but it just looks different around the left edges of this photo, is this normal looking?  2 plates did contaminate so it's user error, I think I need a rack in my SAB because when I am working with the bottom few plates I am moving funny




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