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InvisibleLenz
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Re: Cultivation General Discussion [Re: sandman420]
    #27346675 - 06/13/21 10:49 AM (2 years, 7 months ago)

Yeah, I've been racking my brain for some kind of miniscule operating error I could be making and I've tweaked so many tiny things but at this point it has to just be the plastic lids. Gonna test out the filters on some metal lids, otherwise swear this'll be my last time posting about it until I've figured it out lol. Appreciate the patience everybody.

Roar, I'm running 2 23qt Prestos, I figured if my PC wasn't sterilizing properly or getting to the right temp I'd see bacteria more so than mold. And just to respond to your post in the other thread, I had that thought because I was keeping them on shelves in a room that had a good bit of air movement, air condition, lot of other stuff going on so my last two batches I put them straight into a tote from the PC but I still got mold.

That lid setup is fuckin great sandman, do you just move them straight from PC to the flow, inoculate then recap?

Thanks for the input as well AKA, Feaso, Mr Mushie, Crackatoa.


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:chems:


Edited by Lenz (06/13/21 11:11 AM)


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: Lenz]
    #27346700 - 06/13/21 11:22 AM (2 years, 7 months ago)

Yes I cool the cooker in the flow, and remove them in the flow and remove the caps and inoculate and then put the foil back on and slide the o-ring on. You do need to be careful not to puncture or tear the foil though.


--------------------
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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: Lenz] * 1
    #27346713 - 06/13/21 11:31 AM (2 years, 7 months ago)

Quote:

Lenz said:
What's yr lid setup like for LC? Thinking of springing for the Whatman filters.


Also: Super quick question for flowhood folks.

If you're opening a plate and need to set the lid down, would you put it face down on the elevated rack or face up or on a alc paper towel on the rack? Closest to the filter face of course. Feel like face up would be best but I don't know if contams could potentially get on the lid due to the air flowing around the sides of the lid which could have contams on them.



Why would you need to set the lid down ever?

I flame my scalpel, open the receiving dish lid with my left hand, cool scalpel and replace lid.

Then I take the donor dish lid off with my left hand and lift, pull it back,
then use the edge of the lid to push down to hold the donor plate still while cutting the transfer. Replace lid

Same with agar to grain. The lid never touches a surface and stays in my left
hand the entire time. I wouldnt set a lid down


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: Lenz]
    #27346728 - 06/13/21 11:50 AM (2 years, 7 months ago)

Quote:

Lenz said:
It's almost always dusty fuzzy green mold that pretty much always starts at the top of the jar. Very rarely is it some nasty yellow mold as well.

1-3 weeks after PCing, shows up without fail in at least 30% of my jars. Uninoculated, PC allowed to cool all the way down before opening, lids screwed on tight when removing from PC, then onto shelf or in a tote. I thought maybe keeping them in a tote could help somehow but nope.




If the filters get wet through that's what can happen - mold germinates on the top of the filter and grows through to the inside where it releases spores.  Been there, done with that - cover the lids with foil to keep them from being saturated. :cookiemonster:


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Invisiblesandman420
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Re: Cultivation General Discussion [Re: natedawgnow]
    #27346733 - 06/13/21 11:59 AM (2 years, 7 months ago)

Quote:

natedawgnow said:

Why would you need to set the lid down ever?






I have shaky hands and it helps to kinda prop them with my other hand sometimes.

Or I need my other hand to remove lids of LC jars or move bags around and don't want to put the lid on and off 100 times.

It's just a simple lift, move back and set down for me on the rack behind the plate. No problem. Nothing touches the lid anyway.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (06/13/21 12:00 PM)


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OfflinePitcherCrab
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Re: Cultivation General Discussion [Re: sandman420]
    #27346766 - 06/13/21 12:31 PM (2 years, 7 months ago)

I am with natedawg on this one. I never ever set down a petri lid. I've learned to hold it and use my over fingers to stabilize plates for cutting wedges etc. Putting down the lid is just too much of a vector for contams.


--------------------
PC's LAGM 2021 (TOC & TWC)
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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: sandman420]
    #27346779 - 06/13/21 12:42 PM (2 years, 7 months ago)

Quote:

sandman420 said:
Quote:

natedawgnow said:

Why would you need to set the lid down ever?






I have shaky hands and it helps to kinda prop them with my other hand sometimes.

Or I need my other hand to remove lids of LC jars or move bags around and don't want to put the lid on and off 100 times.

It's just a simple lift, move back and set down for me on the rack behind the plate. No problem. Nothing touches the lid anyway.



The rim of the lid will always have to go over the media in order to put the
lid back on the dish. So if you set the lid down on a surface where the rim
comes into contact with contams then you can potentially spread those contams
over your agar when replacing the lid.

Also pre-unscrewing lids is a great practice. I unscrew lids and leave them
seated on the jar right after wiping them down. That way you dont have
to fuck with lids when doing a2g or g2g.

If it works then thats good. I definitely wouldnt set down a receiving dish
lid. Maybe a transfer dish if I absolutely had to


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: natedawgnow]
    #27346839 - 06/13/21 01:38 PM (2 years, 7 months ago)

I should probably work on holding the lids but I almost always set it facing up on a stack of other plates. I usually like to hold the plate I’m cutting a wedge from almost sideways, no idea how that started.

Putting a transfer to a new plate I just crack the lid a tiny bit.



:shrug:  I hated cutting one handed in the SAB, love the hood.


--------------------
LAGM2020


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: A.k.a]
    #27346848 - 06/13/21 01:50 PM (2 years, 7 months ago)

For agar transfers I like to hold the lid of the dish on the rim of it to keep it from sliding around while cutting a wedge.


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



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InvisibleLenz
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Re: Cultivation General Discussion [Re: TedsDead]
    #27346882 - 06/13/21 02:34 PM (2 years, 7 months ago)

Quote:

PrimalSoup said:
If the filters get wet through that's what can happen - mold germinates on the top of the filter and grows through to the inside where it releases spores.  Been there, done with that - cover the lids with foil to keep them from being saturated. :cookiemonster:




I thought the PTFE filters were hydrophobic? I wasn't using foil for a bit cause I heard it's not necessary with SFD's but went back to using it to see if that changed my contam rate at all. No such luck unfortunately.

Re: setting lids down. I'm definitely sketch putting the lid down ever but I got shaky hands as well and haven't figured a good way to cut out a half circle of a plate or anything besides little transfers really without taking the lid off. And I do like picking up the plate for big wedges and stuff as well. Lid face up in first air on a stack of plates seems pretty safe, I would be a bit worried about the rim of the lid picking up stuff if placed face down on the rack but seems to work for people too.


--------------------
:chems:


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OfflineEugene Gesuale
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Re: Cultivation General Discussion [Re: roarkell]
    #27346966 - 06/13/21 03:58 PM (2 years, 7 months ago)

Quote:

roarkell said:
Quote:

Lenz said:
I strain off most of the sunflower seeds that come up during soak but I've read TC's say sunflower seeds aren't really a big deal and that they're not more likely to contam than anything else. Only thing I can think of is that maybe the sunflower seeds aren't getting hydrated fully and thus not getting sterilized properly? Even then any mold spores really should be dead as fuck after 2 hours at 18PSI sunflower seeds or not.

And yeah venting 15 minutes.

Bit of a head scratcher, idk if I'm missing something super obvious I've considered so many possibilities or if I'm just not meant to use plastic lids lol :shrug: Regardless thanks for the input




What kind of PC do you have? I had some issues with grain jar contams when I first switched from my presto to my AAs... I vent for a minimum of 30 minutes now and no longer have issues




I vent my AA until it reaches 5psi. Which can take anywhere from 5 min to 15 depending on the load. I’m sure if I had it packed full of bags it would need 30


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Offlineroarkell
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Re: Cultivation General Discussion [Re: Eugene Gesuale]
    #27347041 - 06/13/21 05:29 PM (2 years, 7 months ago)

Quote:

Adrenognome said:
Quote:

roarkell said:
Quote:

Lenz said:
I strain off most of the sunflower seeds that come up during soak but I've read TC's say sunflower seeds aren't really a big deal and that they're not more likely to contam than anything else. Only thing I can think of is that maybe the sunflower seeds aren't getting hydrated fully and thus not getting sterilized properly? Even then any mold spores really should be dead as fuck after 2 hours at 18PSI sunflower seeds or not.

And yeah venting 15 minutes.

Bit of a head scratcher, idk if I'm missing something super obvious I've considered so many possibilities or if I'm just not meant to use plastic lids lol :shrug: Regardless thanks for the input




What kind of PC do you have? I had some issues with grain jar contams when I first switched from my presto to my AAs... I vent for a minimum of 30 minutes now and no longer have issues




I vent my AA until it reaches 5psi. Which can take anywhere from 5 min to 15 depending on the load. I’m sure if I had it packed full of bags it would need 30




My stove also has tiny baby balls... so it takes forever to get up to temp... it holds temp nicely tho... lol


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InvisibleFeasoghorm

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Posts: 4,384
Re: Cultivation General Discussion [Re: roarkell]
    #27347122 - 06/13/21 06:43 PM (2 years, 7 months ago)

Indeed. It's jst giving me clues. If your slack with your pcing then shit will be hidden in the sfs of wbs. Actually 2mrrw imma do a 100% sfs jar for fun. And i totally cut wedges holding the plate on its side. That way there isn't anything over the plate that cud drop nasties on it.

Hey Lenz, are you sure your flowhood isnt crapping out death spores?


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OfflineIrishmule169
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Re: Cultivation General Discussion [Re: Lenz]
    #27347160 - 06/13/21 07:22 PM (2 years, 7 months ago)

Quote:

Lenz said:
What's yr lid setup like for LC? Thinking of springing for the Whatman filters.
.






Here’s my LC and spawn jar/ lid set ups..copied ideas from others of course ..
Always cover my spawn jars with foil too during PC also ..


--------------------


Edited by Irishmule169 (06/13/21 07:28 PM)


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: Lenz]
    #27347220 - 06/13/21 08:17 PM (2 years, 7 months ago)

Quote:

Lenz said:
Quote:

PrimalSoup said:
If the filters get wet through that's what can happen - mold germinates on the top of the filter and grows through to the inside where it releases spores.  Been there, done with that - cover the lids with foil to keep them from being saturated. :cookiemonster:




I thought the PTFE filters were hydrophobic? I wasn't using foil for a bit cause I heard it's not necessary with SFD's but went back to using it to see if that changed my contam rate at all. No such luck unfortunately.







Hydrophobic won't save your bacon if they're wet.  Especially if there's any sort of nutrient in them to draw the mold.  I was losing about half of my jars to this problem at one time before I figured it out, but what I actually changed was I went from a full filter under a cap with a hole to a small square RTVd on top of the cap.

Even with that I was still getting some mold occasionally, so I got some silicone gaskets for under the lids. THAT was the key to no infiltration.

IME foil isn't needed if you inoculate immediately but I rarely do that, and with the foil on keeping crap out they can sit around anywhere for weeks - won't dry out and will be cleaner when you go to innoc.  I've left jars sitting out for months with no problem. :lol:

Here's what I mean: 

You could do the lid with plastic wrap when you take it out of the PC, probably cheaper than foil but not as durable.

Anyway, because you say it starts from the top that means it's sneaking in from somewhere at the top - eliminate all the vectors. :shrug:


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 


Edited by PrimalSoup (06/13/21 08:38 PM)


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OfflineSankhara
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Re: Cultivation General Discussion [Re: PrimalSoup]
    #27347985 - 06/14/21 01:49 PM (2 years, 7 months ago)

I just bought a 100 pack of 50mm petris because coming from no pours thats the diameter I was used to work with.


What do you people think? And why?

50mm or 90mm?


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OfflineBrownBear
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Re: Cultivation General Discussion [Re: Sankhara]
    #27348010 - 06/14/21 02:27 PM (2 years, 7 months ago)

I like the 60x15 celltreats with the grip. The 90's or 100's just seem wasteful to me. I don't even use the entire 60 mm plate when I make transfers.


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OfflineSankhara
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Re: Cultivation General Discussion [Re: BrownBear]
    #27348072 - 06/14/21 03:36 PM (2 years, 7 months ago)

Yeah that was my exact thought process!

But I wondered maybe they are more prone to contaminations or something. I guess not thought


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: BrownBear]
    #27348077 - 06/14/21 03:40 PM (2 years, 7 months ago)

I like the bigger ones. But i think it's really just a preference thing and you should just use whatever you're most comfortable with.


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OfflineEugene Gesuale
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Re: Cultivation General Discussion [Re: LtLurker]
    #27348078 - 06/14/21 03:41 PM (2 years, 7 months ago)

When you guys have to choose between taking a clone do you opt for a cluster or a big chonker that stands alone


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