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OfflinePreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 11 hours, 32 minutes
Re: Cultivation General Discussion [Re: SpaceBaby]
    #27325842 - 05/27/21 08:46 PM (2 years, 7 months ago)

What exactly does phenotype mean in mycology?


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: SpaceBaby]
    #27325847 - 05/27/21 08:50 PM (2 years, 7 months ago)

New jars look much better, dude :thumbup:

Primal - Glad i cud be of service.

PrepH - It's physical characteristics.


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OfflinePreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 11 hours, 32 minutes
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27325855 - 05/27/21 08:59 PM (2 years, 7 months ago)

I'm debating some guy he is telling me "the phenotype of this mushroom when dried is cracked and weird looking.


That's every fucking mushroom, they're dried out...  Phenotype means moreso when they're growing right?


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: PreparationH]
    #27325875 - 05/27/21 09:23 PM (2 years, 7 months ago)

Correct


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OfflinePreparationH
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Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 11 hours, 32 minutes
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27325879 - 05/27/21 09:28 PM (2 years, 7 months ago)

Ok that's what I figured, wasting my.time talking to some goober making me.second guess myself, thanks.


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OfflineSpaceBaby
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 4 months, 17 days
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27325887 - 05/27/21 09:32 PM (2 years, 7 months ago)

Quote:

Feasoghorm said:
New jars look much better, dude :thumbup




I am extremely grateful. :bow:

Waiting to see what fruits from that "interesting" set of first jars that are covered in coir in the monotubs.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OfflinePreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 11 hours, 32 minutes
Re: Cultivation General Discussion [Re: SpaceBaby]
    #27326034 - 05/27/21 11:07 PM (2 years, 7 months ago)

I am funemployed for a few weeks, always done pasty plates but wanna start pouring agar in dishes.  What's the go to media container you guys like?  What about dishes?


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: PreparationH] * 1
    #27326040 - 05/27/21 11:14 PM (2 years, 7 months ago)

Verum turned me on to CellTreat with grip ring, by the case.
Wont use any other.


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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house Flag
Last seen: 6 hours, 7 minutes
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Re: Cultivation General Discussion [Re: PreparationH]
    #27326041 - 05/27/21 11:14 PM (2 years, 7 months ago)

Pyrex 500ml media bottles, mine came in a set of 3. Cell treat 100mm with the grip ring. Grip ring being the important part. I started with 60mm but tried 100s and never went back. That's what I use. I have been wanting bigger media bottles though.


--------------------

Crack's Tek's and Shit



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OfflineFailboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 13 hours, 19 seconds
Trusted Cultivator
Re: Cultivation General Discussion [Re: PreparationH]
    #27326042 - 05/27/21 11:19 PM (2 years, 7 months ago)

Just poured a sleeve of 20 today and took transfers. I prep like 3-6 bottles of agar at a time and pour sleeves as needed. I use and wrap with plastic wrap immediately  before labeling strain and date. I use 500ml media bottles, and I recommend paying extra for legit pyrex units. Plates are modestly affordable when bought in cases of 500. I like the 110mm.

For streaks I use like 3 plates with one containing the whole swab end. Take a couple quick transfers from clean vigorous growth and once ya got that uniform culture expand expand expand. 1 master plate has unlimited potential and I used to just discard my old plates after taking samples, so wasteful :facepalm:

I can do without grip rings, but tbh idk if my current plates have em or not. I often order based on size cost and primarily shipping. Free fast shipping with prime is often my go to as horrible as that may be.


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Cultivation General Discussion [Re: Crackatoa]
    #27326043 - 05/27/21 11:20 PM (2 years, 7 months ago)

1 liter of agar at 2% agar and 2% malt in a liquor bottle. Sterilized then poured into celltreat.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Crackatoa]
    #27326045 - 05/27/21 11:21 PM (2 years, 7 months ago)

I use a 1L media bottle and i kinda wish i got 500's. Wudda been better with mine fat irish paws.


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OfflinePreparationH
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Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 11 hours, 32 minutes
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27326056 - 05/27/21 11:32 PM (2 years, 7 months ago)



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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 2 months, 25 days
Re: Cultivation General Discussion [Re: PreparationH] * 1
    #27326081 - 05/27/21 11:58 PM (2 years, 7 months ago)

That's a solid deal on the Pyrex bottles Prep.

If I may suggest, consider getting plates from Kartersci. $45 for a 500ct of 90x15mm. They're good plates, and you can get bundle those 500ml Pyrex bottles for $9 each.

You'll see the shipping charge is fairly high but if you do the math, it's still the cheapest 500ct of plates that you'll find anywhere.

I've used 1000 or so thus far. No complaints besides the occasional cracked plate here and there.


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OfflinePreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 11 hours, 32 minutes
Re: Cultivation General Discussion [Re: Mr. Mushie]
    #27326099 - 05/28/21 12:23 AM (2 years, 7 months ago)

Hey fuck yea, saved me $8, worth it.  Just ordered the 2 500mL bottles and a 500 count of dishes.  Thanks, +5


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OfflineBrian Jones
Club 27
Male User Gallery


Registered: 12/18/12
Posts: 12,342
Loc: attending Snake Church
Last seen: 17 hours, 18 minutes
Re: Cultivation General Discussion [Re: Feasoghorm]
    #27326277 - 05/28/21 06:37 AM (2 years, 7 months ago)

Quote:

Feasoghorm said:
Quote:

DERRAYLD said:
It's  aaaagaaaa



Lol Derrayld is a new englander me thnks

"Core"... wtf AKA, it's 'coy-er'.




I never knew that but you're right. I always thought it was 'qwar'.


--------------------
"The Rolling Stones will break up over Brian Jones' dead body"    John Lennon

I don't want no commies in my car. No Christians either.

The worst thing about corruption is that it works so well,


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OfflineDERRAYLD
Constructus
Male User Gallery
Registered: 05/13/02
Posts: 9,289
Loc: South Africa
Last seen: 28 minutes, 59 seconds
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Re: Cultivation General Discussion [Re: Brian Jones]
    #27326286 - 05/28/21 06:42 AM (2 years, 7 months ago)

The fuck.... qwar?

Cooo aaar?

Or pronounce it like gwar?


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OfflineA.k.aM
Stranger
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 7 hours, 25 minutes
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Re: Cultivation General Discussion [Re: DERRAYLD]
    #27326298 - 05/28/21 06:49 AM (2 years, 7 months ago)

Like a church choir??


You’ll love pouring plates man. It’s the easiest part of cult imo. 30 seconds and you’ve got 20 plates with way better visibility than no pours.


--------------------
LAGM2020


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Cultivation General Discussion [Re: DERRAYLD]
    #27326307 - 05/28/21 06:52 AM (2 years, 7 months ago)

Ha. This cracks me up. Every time I meet someone who has read all these terms but never used them in day to day talk I hear the goofiest shit. Just say Quaw. Like squaw but with a french accent.


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OfflineDERRAYLD
Constructus
Male User Gallery
Registered: 05/13/02
Posts: 9,289
Loc: South Africa
Last seen: 28 minutes, 59 seconds
Trusted Cultivator
Re: Cultivation General Discussion [Re: verum subsequentis]
    #27326311 - 05/28/21 06:53 AM (2 years, 7 months ago)

Here's an idea..... call it coco.:grin:


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